Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum

Heider S, Wolf N, Hofemeier A, Peters-Wendisch P, Wendisch VF (2014)
Frontiers in Bioengineering and Biotechnology 2: 28.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The biotechnologically relevant bacterium Corynebacterium glutamicum, currently used for the million ton-scale production of amino acids for the food and feed industries, is pigmented due to synthesis of the rare cyclic C50 carotenoid decaprenoxanthin and its glucosides. The precursors of carotenoid biosynthesis, isopenthenyl pyrophosphate (IPP) and its isomer dimethylallyl pyrophosphate, are synthesized in this organism via the methylerythritol phosphate (MEP) or non-mevalonate pathway. Terminal pathway engineering in recombinant C. glutamicum permitted the production of various non-native C50 and C40 carotenoids. Here, the role of engineering isoprenoid precursor supply for lycopene production by C. glutamicum was characterized. Overexpression of dxs encoding the enzyme that catalyzes the first committed step of the MEP-pathway by chromosomal promoter exchange in a prophage-cured, genome-reduced C. glutamicum strain improved lycopene formation. Similarly, an increased IPP supply was achieved by chromosomal integration of two artificial operons comprising MEP pathway genes under the control of a constitutive promoter. Combined overexpression of dxs and the other six MEP pathways genes in C. glutamicum strain LYC3-MEP was not synergistic with respect to improving lycopene accumulation. Based on C. glutamicum strain LYC3-MEP, astaxanthin could be produced in the milligrams per gram cell dry weight range when the endogenous genes crtE, crtB, and crtI for conversion of geranylgeranyl pyrophosphate to lycopene were coexpressed with the genes for lycopene cyclase and β-carotene hydroxylase from Pantoea ananatis and carotene C(4) oxygenase from Brevundimonas aurantiaca.
MEP pathway; astaxanthin; carotenoid production; genome-reduced Corynebacterium glutamicum; synthetic operons
Frontiers in Bioengineering and Biotechnology
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
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Heider S, Wolf N, Hofemeier A, Peters-Wendisch P, Wendisch VF. Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology. 2014;2: 28.
Heider, S., Wolf, N., Hofemeier, A., Peters-Wendisch, P., & Wendisch, V. F. (2014). Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology, 2, 28. doi:10.3389/fbioe.2014.00028
Heider, Sabine, Wolf, Natalie, Hofemeier, Arne, Peters-Wendisch, Petra, and Wendisch, Volker F. 2014. “Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum”. Frontiers in Bioengineering and Biotechnology 2: 28.
Heider, S., Wolf, N., Hofemeier, A., Peters-Wendisch, P., and Wendisch, V. F. (2014). Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology 2:28.
Heider, S., et al., 2014. Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology, 2: 28.
S. Heider, et al., “Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum”, Frontiers in Bioengineering and Biotechnology, vol. 2, 2014, : 28.
Heider, S., Wolf, N., Hofemeier, A., Peters-Wendisch, P., Wendisch, V.F.: Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology. 2, : 28 (2014).
Heider, Sabine, Wolf, Natalie, Hofemeier, Arne, Peters-Wendisch, Petra, and Wendisch, Volker F. “Optimization of the IPP precursor supply for the production of lycopene, decaprenoxanthin and astaxanthin by Corynebacterium glutamicum”. Frontiers in Bioengineering and Biotechnology 2 (2014): 28.
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Schwentner A, Feith A, Münch E, Stiefelmaier J, Lauer I, Favilli L, Massner C, Öhrlein J, Grund B, Hüser A, Takors R, Blombach B., Biotechnol Biofuels 12(), 2019
PMID: 30962820
Identifying and engineering the ideal microbial terpenoid production host.
Moser S, Pichler H., Appl Microbiol Biotechnol 103(14), 2019
PMID: 31129740
Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum.
Henke NA, Wichmann J, Baier T, Frohwitter J, Lauersen KJ, Risse JM, Peters-Wendisch P, Kruse O, Wendisch VF., Genes (Basel) 9(4), 2018
PMID: 29673223
Transport and metabolic engineering of the cell factory Corynebacterium glutamicum.
Pérez-García F, Wendisch VF., FEMS Microbiol Lett 365(16), 2018
PMID: 29982619
Synthetic biology approaches to access renewable carbon source utilization in Corynebacterium glutamicum.
Zhao N, Qian L, Luo G, Zheng S., Appl Microbiol Biotechnol 102(22), 2018
PMID: 30218378
Fermentative production of L-pipecolic acid from glucose and alternative carbon sources.
Pérez-García F, Max Risse J, Friehs K, Wendisch VF., Biotechnol J 12(7), 2017
PMID: 28169491
Insight of Genus Corynebacterium: Ascertaining the Role of Pathogenic and Non-pathogenic Species.
Oliveira A, Oliveira LC, Aburjaile F, Benevides L, Tiwari S, Jamal SB, Silva A, Figueiredo HCP, Ghosh P, Portela RW, De Carvalho Azevedo VA, Wattam AR., Front Microbiol 8(), 2017
PMID: 29075239
Synthetic and systems biology for microbial production of commodity chemicals.
Chubukov V, Mukhopadhyay A, Petzold CJ, Keasling JD, Martín HG., NPJ Syst Biol Appl 2(), 2016
PMID: 28725470
Updates on industrial production of amino acids using Corynebacterium glutamicum.
Wendisch VF, Jorge JMP, Pérez-García F, Sgobba E., World J Microbiol Biotechnol 32(6), 2016
PMID: 27116971
Spatial organization of heterologous metabolic system in vivo based on TALE.
Zhu LY, Qiu XY, Zhu LY, Wu XM, Zhang Y, Zhu QH, Fan DY, Zhu CS, Zhang DY., Sci Rep 6(), 2016
PMID: 27184291
Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum.
Henke NA, Heider SA, Peters-Wendisch P, Wendisch VF., Mar Drugs 14(7), 2016
PMID: 27376307
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum.
Binder D, Frohwitter J, Mahr R, Bier C, Grünberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger KE, Wendisch VF, Drepper T., Appl Environ Microbiol 82(20), 2016
PMID: 27520809
Editorial - Synthetic Biology: Engineering Complexity and Refactoring Cell Capabilities.
Ceroni F, Carbonell P, François JM, Haynes KA., Front Bioeng Biotechnol 3(), 2015
PMID: 26347864
Developments in the tools and methodologies of synthetic biology.
Kelwick R, MacDonald JT, Webb AJ, Freemont P., Front Bioeng Biotechnol 2(), 2014
PMID: 25505788

90 References

Daten bereitgestellt von Europe PubMed Central.

Taxonomical studies on glutamic acid producing bacteria
Abe S., Takayarna K., Kinoshita S.., 1967
Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter.
Abraham WR, Strompl C, Meyer H, Lindholst S, Moore ER, Christ R, Vancanneyt M, Tindall BJ, Bennasar A, Smit J, Tesar M., Int. J. Syst. Bacteriol. 49 Pt 3(), 1999
PMID: 10425763
Biosynthesis of terpenes: studies on 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase.
Adam P, Hecht S, Eisenreich W, Kaiser J, Grawert T, Arigoni D, Bacher A, Rohdich F., Proc. Natl. Acad. Sci. U.S.A. 99(19), 2002
PMID: 12198182
Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli.
Ajikumar PK, Xiao WH, Tyo KE, Wang Y, Simeon F, Leonard E, Mucha O, Phon TH, Pfeifer B, Stephanopoulos G., Science 330(6000), 2010
PMID: 20929806
Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 for use as a platform strain for basic research and industrial biotechnology.
Baumgart M, Unthan S, Ruckert C, Sivalingam J, Grunberger A, Kalinowski J, Bott M, Noack S, Frunzke J., Appl. Environ. Microbiol. 79(19), 2013
PMID: 23892752

Thiamin biosynthesis in prokaryotes.
Begley TP, Downs DM, Ealick SE, McLafferty FW, Van Loon AP, Taylor S, Campobasso N, Chiu HJ, Kinsland C, Reddick JJ, Xi J., Arch. Microbiol. 171(5), 1999
PMID: 10382260
Microbial xanthophylls.
Bhosale P, Bernstein PS., Appl. Microbiol. Biotechnol. 68(4), 2005
PMID: 16001255
From crude glycerol to carotenoids by using a Rhodotorula glutinis mutant
Cutzu R, Coi A, Rosso F, Bardi L, Ciani M, Budroni M, Zara G, Zara S, Mannazzu I., World J. Microbiol. Biotechnol. 29(6), 2013
PMID: IND500664511
An update on microbial carotenoid production: application of recent metabolic engineering tools.
Das A, Yoon SH, Lee SH, Kim JY, Oh DK, Kim SW., Appl. Microbiol. Biotechnol. 77(3), 2007
PMID: 17912511
Colouring our foods in the last and next millennium.
Downham A, Collins P., International journal of food science and technology. 35(1), 2000
PMID: IND22073425

Eggeling L., Reyes O.., 2005
Production of the sesquiterpene (+)-valencene by metabolically engineered Corynebacterium glutamicum.
Frohwitter J, Heider SA, Peters-Wendisch P, Beekwilder J, Wendisch VF., J. Biotechnol. 191(), 2014
PMID: 24910970
Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions.
Fukui K, Koseki C, Yamamoto Y, Nakamura J, Sasahara A, Yuji R, Hashiguchi K, Usuda Y, Matsui K, Kojima H, Abe K., J. Biotechnol. 154(1), 2011
PMID: 21420450
Enzymatic assembly of DNA molecules up to several hundred kilobases.
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO., Nat. Methods 6(5), 2009
PMID: 19363495
Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum.
Gopinath V, Meiswinkel TM, Wendisch VF, Nampoothiri KM., Appl. Microbiol. Biotechnol. 92(5), 2011
PMID: 21796382
Biochemistry of the non-mevalonate isoprenoid pathway.
Grawert T, Groll M, Rohdich F, Bacher A, Eisenreich W., Cell. Mol. Life Sci. 68(23), 2011
PMID: 21744068
IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis.
Grawert T, Kaiser J, Zepeck F, Laupitz R, Hecht S, Amslinger S, Schramek N, Schleicher E, Weber S, Haslbeck M, Buchner J, Rieder C, Arigoni D, Bacher A, Eisenreich W, Rohdich F., J. Am. Chem. Soc. 126(40), 2004
PMID: 15469281
Studies on transformation of Escherichia coli with plasmids.
Hanahan D., J. Mol. Biol. 166(4), 1983
PMID: 6345791
Novel approaches and achievements in biosynthesis of functional isoprenoids in Escherichia coli.
Harada H, Misawa N., Appl. Microbiol. Biotechnol. 84(6), 2009
PMID: 19672590
Production and glucosylation of C50 and C 40 carotenoids by metabolically engineered Corynebacterium glutamicum.
Heider SA, Peters-Wendisch P, Netzer R, Stafnes M, Brautaset T, Wendisch VF., Appl. Microbiol. Biotechnol. 98(3), 2013
PMID: 24270893
Metabolic engineering for the microbial production of carotenoids and related products with a focus on the rare C50 carotenoids.
Heider SA, Peters-Wendisch P, Wendisch VF, Beekwilder J, Brautaset T., Appl. Microbiol. Biotechnol. 98(10), 2014
PMID: 24687754
Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum.
Heider SA, Peters-Wendisch P, Wendisch VF., BMC Microbiol. 12(), 2012
PMID: 22963379
Astaxanthin: a review of its chemistry and applications.
Higuera-Ciapara I, Felix-Valenzuela L, Goycoolea FM., Crit Rev Food Sci Nutr 46(2), 2006
PMID: 16431409
The biogenetic anatomy of vitamin B6. A 13C NMR investigation of the biosynthesis of pyridoxol in Escherichia coli.
Hill RE, Himmeldirk K, Kennedy IA, Pauloski RM, Sayer BG, Wolf E, Spenser ID., J. Biol. Chem. 271(48), 1996
PMID: 8940007
Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation.
Holatko J, Elisakova V, Prouza M, Sobotka M, Nesvera J, Patek M., J. Biotechnol. 139(3), 2008
PMID: 19121344
The chemistry of novel xanthophyll carotenoids.
Jackson H, Braun CL, Ernst H., Am. J. Cardiol. 101(10A), 2008
PMID: 18474275

Jacobson G., Jolly S., Sedmak J., Skatrud T., Wasileski J.., 1999
Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum.
Jensen JV, Wendisch VF., Microb. Cell Fact. 12(), 2013
PMID: 23806148
Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs.
Jomaa H, Wiesner J, Sanderbrand S, Altincicek B, Weidemeyer C, Hintz M, Turbachova I, Eberl M, Zeidler J, Lichtenthaler HK, Soldati D, Beck E., Science 285(5433), 1999
PMID: 10477522
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Julsing MK, Rijpkema M, Woerdenbag HJ, Quax WJ, Kayser O., Appl. Microbiol. Biotechnol. 75(6), 2007
PMID: 17458547
Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli.
Kajiwara S, Fraser PD, Kondo K, Misawa N., Biochem. J. 324 ( Pt 2)(), 1997
PMID: 9182699
Detailed biosynthetic pathway to decaprenoxanthin diglucoside in Corynebacterium glutamicum and identification of novel intermediates.
Krubasik P, Takaichi S, Maoka T, Kobayashi M, Masamoto K, Sandmann G., Arch. Microbiol. 176(3), 2001
PMID: 11511870
Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes.
Lange BM, Rujan T, Martin W, Croteau R., Proc. Natl. Acad. Sci. U.S.A. 97(24), 2000
PMID: 11078528
Metabolic engineering towards biotechnological production of carotenoids in microorganisms.
Lee PC, Schmidt-Dannert C., Appl. Microbiol. Biotechnol. 60(1-2), 2002
PMID: 12382037
Commercial potential for Haematococcus microalgae as a natural source of astaxanthin.
Lorenz RT, Cysewski GR., Trends Biotechnol. 18(4), 2000
PMID: 10740262
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids.
Martin VJ, Pitera DJ, Withers ST, Newman JD, Keasling JD., Nat. Biotechnol. 21(7), 2003
PMID: 12778056
Engineering of Corynebacterium glutamicum for growth and L-lysine and lycopene production from N-acetyl-glucosamine.
Matano C, Uhde A, Youn JW, Maeda T, Clermont L, Marin K, Kramer R, Wendisch VF, Seibold GM., Appl. Microbiol. Biotechnol. 98(12), 2014
PMID: 24668244
Crude glycerol-based production of amino acids and putrescine by Corynebacterium glutamicum.
Meiswinkel TM, Rittmann D, Lindner SN, Wendisch VF., Bioresour. Technol. 145(), 2013
PMID: 23562176
Biological functions and activities of animal carotenoids
Miki W.., 1991
Metabolic engineering of Corynebacterium glutamicum for cadaverine fermentation.
Mimitsuka T, Sawai H, Hatsu M, Yamada K., Biosci. Biotechnol. Biochem. 71(9), 2007
PMID: 17895539
Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli.
Misawa N, Nakagawa M, Kobayashi K, Yamano S, Izawa Y, Nakamura K, Harashima K., J. Bacteriol. 172(12), 1990
PMID: 2254247
Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly.
Nowroozi FF, Baidoo EE, Ermakov S, Redding-Johanson AM, Batth TS, Petzold CJ, Keasling JD., Appl. Microbiol. Biotechnol. 98(4), 2013
PMID: 24257840
Identification and microbial production of a terpene-based advanced biofuel.
Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS., Nat Commun 2(), 2011
PMID: 21952217
Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum.
Peters-Wendisch PG, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ., J. Mol. Microbiol. Biotechnol. 3(2), 2001
PMID: 11321586
fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis.
Puan KJ, Wang H, Dairi T, Kuzuyama T, Morita CT., FEBS Lett. 579(17), 2005
PMID: 15978585
High-cell-density cultivation of microorganisms.
Riesenberg D, Guthke R., Appl. Microbiol. Biotechnol. 51(4), 1999
PMID: 10341426
Engineering of a glycerol utilization pathway for amino acid production by Corynebacterium glutamicum.
Rittmann D, Lindner SN, Wendisch VF., Appl. Environ. Microbiol. 74(20), 2008
PMID: 18757581
Xanthophyllomyces dendrorhous for the industrial production of astaxanthin.
Rodriguez-Saiz M, de la Fuente JL, Barredo JL., Appl. Microbiol. Biotechnol. 88(3), 2010
PMID: 20711573
Carotenoid accumulation in bacteria with enhanced supply of isoprenoid precursors by upregulation of exogenous or endogenous pathways.
Rodriguez-Villalon A, Perez-Gil J, Rodriguez-Concepcion M., J. Biotechnol. 135(1), 2008
PMID: 18417238
Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein.
Rohdich F, Hecht S, Gartner K, Adam P, Krieger C, Amslinger S, Arigoni D, Bacher A, Eisenreich W., Proc. Natl. Acad. Sci. U.S.A. 99(3), 2002
PMID: 11818558
Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate.
Rohmer M, Knani M, Simonin P, Sutter B, Sahm H., Biochem. J. 295 ( Pt 2)(), 1993
PMID: 8240251
Effect of lignocellulose-derived inhibitors on growth of and ethanol production by growth-arrested Corynebacterium glutamicum R.
Sakai S, Tsuchida Y, Nakamoto H, Okino S, Ichihashi O, Kawaguchi H, Watanabe T, Inui M, Yukawa H., Appl. Environ. Microbiol. 73(7), 2007
PMID: 17277203

Sambrook J., Russell D.., 2001
Identification of lethal mutations in Escherichia coli genes encoding enzymes of the methylerythritol phosphate pathway.
Sauret-Gueto S, Ramos-Valdivia A, Ibanez E, Boronat A, Rodriguez-Concepcion M., Biochem. Biophys. Res. Commun. 307(2), 2003
PMID: 12859972
Acetyl-CoA synthetase (ADP forming) in archaea, a novle enzyme involved in acetate formation and ATP synthesis
Schäfer T., Selig M., Schönheit P.., 1993
Biotechnological production of astaxanthin with Phaffia rhodozyma/Xanthophyllomyces dendrorhous.
Schmidt I, Schewe H, Gassel S, Jin C, Buckingham J, Humbelin M, Sandmann G, Schrader J., Appl. Microbiol. Biotechnol. 89(3), 2010
PMID: 21046372
Putrescine production by engineered Corynebacterium glutamicum.
Schneider J, Wendisch VF., Appl. Microbiol. Biotechnol. 88(4), 2010
PMID: 20661733
Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: growth and lysine production.
Seibold G, Auchter M, Berens S, Kalinowski J, Eikmanns BJ., J. Biotechnol. 124(2), 2006
PMID: 16488498
Evidence of isoprenoid precursor toxicity in Bacillus subtilis.
Sivy TL, Fall R, Rosenstiel TN., Biosci. Biotechnol. Biochem. 75(12), 2011
PMID: 22146731
Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol.
Sprenger GA, Schorken U, Wiegert T, Grolle S, de Graaf AA, Taylor SV, Begley TP, Bringer-Meyer S, Sahm H., Proc. Natl. Acad. Sci. U.S.A. 94(24), 1997
PMID: 9371765
Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production.
Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF., Appl. Environ. Microbiol. 71(10), 2005
PMID: 16204505
Production of rubber-like polymers by microorganisms.
Steinbuchel A., Curr. Opin. Microbiol. 6(3), 2003
PMID: 12831902
The methylerythritol phosphate pathway and its significance as a novel drug target.
Testa CA, Brown MJ., Curr Pharm Biotechnol 4(4), 2003
PMID: 14529427
Glutamate production from β-glucan using endoglucanase-secreting Corynebacterium glutamicum.
Tsuchidate T, Tateno T, Okai N, Tanaka T, Ogino C, Kondo A., Appl. Microbiol. Biotechnol. 90(3), 2011
PMID: 21305281
Glucosamine as carbon source for amino acid-producing Corynebacterium glutamicum.
Uhde A, Youn JW, Maeda T, Clermont L, Matano C, Kramer R, Wendisch VF, Seibold GM, Marin K., Appl. Microbiol. Biotechnol. 97(4), 2012
PMID: 22854894
Citrate synthase in Corynebacterium glutamicum is encoded by two gltA transcripts which are controlled by RamA, RamB, and GlxR.
van Ooyen J, Emer D, Bussmann M, Bott M, Eikmanns BJ, Eggeling L., J. Biotechnol. 154(2-3), 2010
PMID: 20630483
Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity.
Withers ST, Gottlieb SS, Lieu B, Newman JD, Keasling JD., Appl. Environ. Microbiol. 73(19), 2007
PMID: 17693564
Crystal structure of 1-deoxy-D-xylulose 5-phosphate synthase, a crucial enzyme for isoprenoids biosynthesis.
Xiang S, Usunow G, Lange G, Busch M, Tong L., J. Biol. Chem. 282(4), 2006
PMID: 17135236
Spanning high-dimensional expression space using ribosome-binding site combinatorics.
Zelcbuch L, Antonovsky N, Bar-Even A, Levin-Karp A, Barenholz U, Dayagi M, Liebermeister W, Flamholz A, Noor E, Amram S, Brandis A, Bareia T, Yofe I, Jubran H, Milo R., Nucleic Acids Res. 41(9), 2013
PMID: 23470993
Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway.
Zhao Y, Yang J, Qin B, Li Y, Sun Y, Su S, Xian M., Appl. Microbiol. Biotechnol. 90(6), 2011
PMID: 21468716
In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli.
Zhu F, Zhong X, Hu M, Lu L, Deng Z, Liu T., Biotechnol. Bioeng. 111(7), 2014
PMID: 24473754

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