Microscale immobilized enzyme reactors in proteomics: Latest developments
Safdar M, Sproß J, Jaenis J (2014)
Journal of Chromatography A 1324: 1-10.
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Autor*in
Safdar, Muhammad;
Sproß, JensUniBi;
Jaenis, Janne
Abstract / Bemerkung
Enzymatic digestion of proteins is one of the key steps in proteomic analyses. There has been a steady progress in the applied digestion protocols in the past, starting from conventional time-consuming in-solution or in-gel digestion protocols to rapid and efficient methods utilizing different types of microscale enzyme reactors. Application of such microreactors has been proven beneficial due to lower sample consumption, higher sensitivity and straightforward coupling with LC-MS set-ups. Novel stationary phases, immobilization techniques and device formats are being constantly developed and tested to optimize digestion efficiency of proteolytic enzymes. This review focuses on the latest developments associated with the preparation and application of microscale enzyme reactors for proteomics applications since 2008 onwards. A special attention has been paid to the discussion of different stationary phases applied for immobilization purposes. (C) 2013 Elsevier B.V. All rights reserved.
Stichworte
Monolith;
Miniaturization;
Enzyme immobilization;
Enzyme reactor;
Proteomics;
Microfluidics
Erscheinungsjahr
2014
Zeitschriftentitel
Journal of Chromatography A
Band
1324
Seite(n)
1-10
ISSN
0021-9673
Page URI
https://pub.uni-bielefeld.de/record/2660851
Zitieren
Safdar M, Sproß J, Jaenis J. Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A. 2014;1324:1-10.
Safdar, M., Sproß, J., & Jaenis, J. (2014). Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A, 1324, 1-10. doi:10.1016/j.chroma.2013.11.045
Safdar, Muhammad, Sproß, Jens, and Jaenis, Janne. 2014. “Microscale immobilized enzyme reactors in proteomics: Latest developments”. Journal of Chromatography A 1324: 1-10.
Safdar, M., Sproß, J., and Jaenis, J. (2014). Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A 1324, 1-10.
Safdar, M., Sproß, J., & Jaenis, J., 2014. Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A, 1324, p 1-10.
M. Safdar, J. Sproß, and J. Jaenis, “Microscale immobilized enzyme reactors in proteomics: Latest developments”, Journal of Chromatography A, vol. 1324, 2014, pp. 1-10.
Safdar, M., Sproß, J., Jaenis, J.: Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A. 1324, 1-10 (2014).
Safdar, Muhammad, Sproß, Jens, and Jaenis, Janne. “Microscale immobilized enzyme reactors in proteomics: Latest developments”. Journal of Chromatography A 1324 (2014): 1-10.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
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