Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy
Hagen S, Baumann T, Wagner HJ, Morath V, Kaufmann B, Fischer A, Bergmann S, Schindler P, Arndt KM, Müller K (2014)
Scientific Reports 4(1): 3759.
Zeitschriftenaufsatz
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Autor*in
Hagen, Sven;
Baumann, Tobias;
Wagner, Hanna J.;
Morath, Volker;
Kaufmann, Beate;
Fischer, Adrian;
Bergmann, Stefan;
Schindler, Patrick;
Arndt, Katja M.;
Müller, KristianUniBi 
Abstract / Bemerkung
The pre-clinical and clinical development of viral vehicles for gene transfer increased in recent years, and a recombinant adeno-associated virus (rAAV) drug took center stage upon approval in the European Union. However, lack of standardization, inefficient purification methods and complicated retargeting limit general usability. We address these obstacles by fusing rAAV-2 capsids with two modular targeting molecules (DARPin or Affibody) specific for a cancer cell-surface marker (EGFR) while simultaneously including an affinity tag (His-tag) in a surface-exposed loop. Equipping these particles with genes coding for prodrug converting enzymes (thymidine kinase or cytosine deaminase) we demonstrate tumor marker specific transduction and prodrug-dependent apoptosis of cancer cells. Coding terminal and loop modifications in one gene enabled specific and scalable purification. Our genetic parts for viral production adhere to a standardized cloning strategy facilitating rapid prototyping of virus directed enzyme prodrug therapy (VDEPT).
Erscheinungsjahr
2014
Zeitschriftentitel
Scientific Reports
Band
4
Ausgabe
1
Art.-Nr.
3759
ISSN
2045-2322
eISSN
2045-2322
Page URI
https://pub.uni-bielefeld.de/record/2656744
Zitieren
Hagen S, Baumann T, Wagner HJ, et al. Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy. Scientific Reports. 2014;4(1): 3759.
Hagen, S., Baumann, T., Wagner, H. J., Morath, V., Kaufmann, B., Fischer, A., Bergmann, S., et al. (2014). Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy. Scientific Reports, 4(1), 3759. doi:10.1038/srep03759
Hagen, Sven, Baumann, Tobias, Wagner, Hanna J., Morath, Volker, Kaufmann, Beate, Fischer, Adrian, Bergmann, Stefan, Schindler, Patrick, Arndt, Katja M., and Müller, Kristian. 2014. “Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy”. Scientific Reports 4 (1): 3759.
Hagen, S., Baumann, T., Wagner, H. J., Morath, V., Kaufmann, B., Fischer, A., Bergmann, S., Schindler, P., Arndt, K. M., and Müller, K. (2014). Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy. Scientific Reports 4:3759.
Hagen, S., et al., 2014. Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy. Scientific Reports, 4(1): 3759.
S. Hagen, et al., “Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy”, Scientific Reports, vol. 4, 2014, : 3759.
Hagen, S., Baumann, T., Wagner, H.J., Morath, V., Kaufmann, B., Fischer, A., Bergmann, S., Schindler, P., Arndt, K.M., Müller, K.: Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy. Scientific Reports. 4, : 3759 (2014).
Hagen, Sven, Baumann, Tobias, Wagner, Hanna J., Morath, Volker, Kaufmann, Beate, Fischer, Adrian, Bergmann, Stefan, Schindler, Patrick, Arndt, Katja M., and Müller, Kristian. “Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy”. Scientific Reports 4.1 (2014): 3759.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
5 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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PMID: 15893564
Recruitment of single-stranded recombinant adeno-associated virus vector genomes and intermolecular recombination are responsible for stable transduction of liver in vivo.
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PMID: 11000214
Nakai H, Storm TA, Kay MA., J. Virol. 74(20), 2000
PMID: 11000214
Genetic analysis of hyperproduction of epidermal growth factor receptors in human epidermoid carcinoma A431 cells.
Shimizu N, Kondo I, Gamou S, Behzadian MA, Shimizu Y., Somat. Cell Mol. Genet. 10(1), 1984
PMID: 6322359
Shimizu N, Kondo I, Gamou S, Behzadian MA, Shimizu Y., Somat. Cell Mol. Genet. 10(1), 1984
PMID: 6322359
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