A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis

Löhr B, Streitner C, Steffen A, Lange T, Staiger D (2014)
Molecular Biology Reports 41(1): 439-445.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The RNA-binding protein Arabidopsis thaliana glycine-rich RNA-binding protein 7 (AtGRP7) regulates the steady-state abundance of numerous target transcripts in A. thaliana. Here we show that the GA1 and GA2 transcripts encoding the first enzymes of the gibberellin biosynthetic pathway are expressed at reduced levels in transgenic plants ectopically over-expressing AtGRP7 (AtGRP7-ox plants). Furthermore, the levels of the bioactive phytohormone GA(4) as well as of several intermediates of the GA biosynthetic pathway are reduced in AtGRP7-ox plants. The transgenic plants show a reduced length of the vegetative stem. The application of exogenous GA largely reverses the phenotype by increasing the number of vegetative internodes. AtGRP7-ox plants flower with fewer leaves than wt plants, suggesting that the floral promotive effect of AtGRP7 bypasses the effect of a reduced GA level in AtGRP7-ox plants. Upon GA treatment, AtGRP7-ox plants flower only slightly earlier than wild type plants. Thus, exogenous GA has only a small additional effect in reducing the number of leaves at the onset of flowering in AtGRP7-ox plants.
Arabidopsis; RNA-binding protein; Gibberellin; Flowering time; Gene expression
Molecular Biology Reports
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Löhr B, Streitner C, Steffen A, Lange T, Staiger D. A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis. Molecular Biology Reports. 2014;41(1):439-445.
Löhr, B., Streitner, C., Steffen, A., Lange, T., & Staiger, D. (2014). A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis. Molecular Biology Reports, 41(1), 439-445. doi:10.1007/s11033-013-2878-7
Löhr, B., Streitner, C., Steffen, A., Lange, T., and Staiger, D. (2014). A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis. Molecular Biology Reports 41, 439-445.
Löhr, B., et al., 2014. A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis. Molecular Biology Reports, 41(1), p 439-445.
B. Löhr, et al., “A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis”, Molecular Biology Reports, vol. 41, 2014, pp. 439-445.
Löhr, B., Streitner, C., Steffen, A., Lange, T., Staiger, D.: A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis. Molecular Biology Reports. 41, 439-445 (2014).
Löhr, Bianca, Streitner, Corinna, Steffen, Alexander, Lange, T., and Staiger, Dorothee. “A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis”. Molecular Biology Reports 41.1 (2014): 439-445.

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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Cao X, Fan G, Dong Y, Zhao Z, Deng M, Wang Z, Liu W., Front Plant Sci 8(), 2017
PMID: 28344590
RNA-Binding Proteins Revisited - The Emerging Arabidopsis mRNA Interactome.
Köster T, Marondedze C, Meyer K, Staiger D., Trends Plant Sci 22(6), 2017
PMID: 28412036
Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7.
Meyer K, Köster T, Nolte C, Weinholdt C, Lewinski M, Grosse I, Staiger D., Genome Biol 18(1), 2017
PMID: 29084609
Identification of potential cargo proteins of transportin protein AtTRN1 in Arabidopsis thaliana.
Yan B, Wang X, Wang Z, Chen N, Mu C, Mao K, Han L, Zhang W, Liu H., Plant Cell Rep 35(3), 2016
PMID: 26650834
Cold-inducible proteins CIRP and RBM3, a unique couple with activities far beyond the cold.
Zhu X, Bührer C, Wellmann S., Cell Mol Life Sci 73(20), 2016
PMID: 27147467
Time to flower: interplay between photoperiod and the circadian clock.
Johansson M, Staiger D., J Exp Bot 66(3), 2015
PMID: 25371508
WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis.
Bakshi M, Vahabi K, Bhattacharya S, Sherameti I, Varma A, Yeh KW, Baldwin I, Johri AK, Oelmüller R., BMC Plant Biol 15(), 2015
PMID: 26718529
HnRNP-like proteins as post-transcriptional regulators.
Yeap WC, Namasivayam P, Ho CL., Plant Sci 227(), 2014
PMID: 25219311
Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis.
Köster T, Meyer K, Weinholdt C, Smith LM, Lummer M, Speth C, Grosse I, Weigel D, Staiger D., Nucleic Acids Res 42(15), 2014
PMID: 25104024

39 References

Daten bereitgestellt von Europe PubMed Central.

A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8.
Schmidt F, Marnef A, Cheung MK, Wilson I, Hancock J, Staiger D, Ladomery M., Mol. Biol. Rep. 37(2), 2009
PMID: 19672695
Structure function analysis of an ADP-ribosyltransferase type III effector and its RNA-binding target in plant immunity.
Jeong BR, Lin Y, Joe A, Guo M, Korneli C, Yang H, Wang P, Yu M, Cerny RL, Staiger D, Alfano JR, Xu Y., J. Biol. Chem. 286(50), 2011
PMID: 22013065
A type III effector ADP-ribosylates RNA-binding proteins and quells plant immunity.
Fu ZQ, Guo M, Jeong BR, Tian F, Elthon TE, Cerny RL, Staiger D, Alfano JR., Nature 447(7142), 2007
PMID: 17450127
Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7.
Nicaise V, Joe A, Jeong BR, Korneli C, Boutrot F, Westedt I, Staiger D, Alfano JR, Zipfel C., EMBO J. 32(5), 2013
PMID: 23395902
Different roles of glycine-rich RNA-binding protein7 in plant defense against Pectobacterium carotovorum, Botrytis cinerea, and tobacco mosaic viruses.
Lee HJ, Kim JS, Yoo SJ, Kang EY, Han SH, Yang KY, Kim YC, McSpadden Gardener B, Kang H., Plant Physiol. Biochem. 60(), 2012
PMID: 22902796
Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7.
Streitner C, Hennig L, Korneli C, Staiger D., BMC Plant Biol. 10(), 2010
PMID: 20946635
Salicylic acid-dependent and -independent impact of an RNA-binding protein on plant immunity.
Hackmann C, Korneli C, Kutyniok M, Koster T, Wiedenlubbert M, Muller C, Staiger D., Plant Cell Environ. 37(3), 2013
PMID: 23961939
The small glycine-rich RNA binding protein AtGRP7 promotes floral transition in Arabidopsis thaliana.
Streitner C, Danisman S, Wehrle F, Schoning JC, Alfano JR, Staiger D., Plant J. 56(2), 2008
PMID: 18573194
The genetic basis of flowering responses to seasonal cues.
Andres F, Coupland G., Nat. Rev. Genet. 13(9), 2012
PMID: 22898651
Regulation of flowering time: all roads lead to Rome.
Srikanth A, Schmid M., Cell. Mol. Life Sci. 68(12), 2011
PMID: 21611891
An hnRNP-like RNA-binding protein affects alternative splicing by in vivo interaction with transcripts in Arabidopsis thaliana.
Streitner C, Koster T, Simpson CG, Shaw P, Danisman S, Brown JW, Staiger D., Nucleic Acids Res. 40(22), 2012
PMID: 23042250
Small changes in ambient temperature affect alternative splicing in Arabidopsis thaliana.
Streitner C, Simpson CG, Shaw P, Danisman S, Brown JW, Staiger D., Plant Signal Behav 8(7), 2013
PMID: 23656882
Gibberellin metabolism and its regulation.
Yamaguchi S., Annu Rev Plant Biol 59(), 2008
PMID: 18173378
Gibberellin as a factor in floral regulatory networks.
Mutasa-Gottgens E, Hedden P., J. Exp. Bot. 60(7), 2009
PMID: 19264752
Gibberellin control of stamen development: a fertile field.
Plackett AR, Thomas SG, Wilson ZA, Hedden P., Trends Plant Sci. 16(10), 2011
PMID: 21824801

C, Front Plant Physiol 2(), 2012
DELLAs control plant immune responses by modulating the balance of jasmonic acid and salicylic acid signaling.
Navarro L, Bari R, Achard P, Lison P, Nemri A, Harberd NP, Jones JD., Curr. Biol. 18(9), 2008
PMID: 18450451
Gibberellin biosynthesis and the regulation of plant development.
Pimenta Lange MJ, Lange T., Plant Biol (Stuttg) 8(3), 2006
PMID: 16807819
High levels of jasmonic acid antagonize the biosynthesis of gibberellins and inhibit the growth of Nicotiana attenuata stems.
Heinrich M, Hettenhausen C, Lange T, Wunsche H, Fang J, Baldwin IT, Wu J., Plant J. 73(4), 2012
PMID: 23190261
GID1-mediated gibberellin signaling in plants.
Hirano K, Ueguchi-Tanaka M, Matsuoka M., Trends Plant Sci. 13(4), 2008
PMID: 18337155
Gibberellins promote flowering of arabidopsis by activating the LEAFY promoter
Blazquez MA, Green R, Nilsson O, Sussman MR, Weigel D., Plant Cell 10(5), 1998
PMID: 9596637
Gibberellin Is Required for Flowering in Arabidopsis thaliana under Short Days.
Wilson RN, Heckman JW, Somerville CR., Plant Physiol. 100(1), 1992
PMID: 16652976
Characterisation of a T-DNA-tagged gene of Arabidopsis thaliana that regulates gibberellin metabolism and flowering time.
Svensson M, Lundh D, Bergman P, Mandal A., Functional plant biology : FPB. 32(10), 2005
PMID: IND43756865
AtGRP7, a nuclear RNA-binding protein as a component of a circadian-regulated negative feedback loop in Arabidopsis thaliana.
Heintzen C, Nater M, Apel K, Staiger D., Proc. Natl. Acad. Sci. U.S.A. 94(16), 1997
PMID: 9238008
The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycle.
Rieu I, Ruiz-Rivero O, Fernandez-Garcia N, Griffiths J, Powers SJ, Gong F, Linhartova T, Eriksson S, Nilsson O, Thomas SG, Phillips AL, Hedden P., Plant J. 53(3), 2007
PMID: 18069939
Gibberellin biosynthesis in developing pumpkin seedlings.
Lange T, Kappler J, Fischer A, Frisse A, Padeffke T, Schmidtke S, Lange MJ., Plant Physiol. 139(1), 2005
PMID: 16126862
AtGRP7 is involved in the regulation of abscisic acid and stress responses in Arabidopsis.
Cao S, Jiang L, Song S, Jing R, Xu G., Cell. Mol. Biol. Lett. 11(4), 2006
PMID: 17001447
Arabidopsis transportin1 is the nuclear import receptor for the circadian clock-regulated RNA-binding protein AtGRP7.
Ziemienowicz A, Haasen D, Staiger D, Merkle T., Plant Mol. Biol. 53(1-2), 2003
PMID: 14756317
Reversible photoswitchable DRONPA-s monitors nucleocytoplasmic transport of an RNA-binding protein in transgenic plants.
Lummer M, Humpert F, Steuwe C, Caesar K, Schuttpelz M, Sauer M, Staiger D., Traffic 12(6), 2011
PMID: 21453442


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