Desensitization and Internalization of Endothelin Receptor A IMPACT OF G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2)-MEDIATED PHOSPHORYLATION

Gaertner F, Seidel T, Schulz U, Gummert J, Milting H (2013)
Journal of Biological Chemistry 288(45): 32138-32148.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Autor/in
Gaertner, Florian; Seidel, ThorstenUniBi; Schulz, Uwe; Gummert, Jan; Milting, Hendrik
Abstract / Bemerkung
Endothelin receptor A (ETA), a G protein-coupled receptor, mediates endothelin signaling, which is regulated by GRK2. Three Ser and seven Thr residues recently proven to be phosphoacceptor sites are located in the C-terminal extremity (CTE) of the receptor following its palmitoylation site. We created various phosphorylation-deficient ETA mutants. The phospholipase C activity of mutant receptors in HEK-293 cells was analyzed during continuous endothelin stimulation to investigate the impact of phosphorylation sites on ETA desensitization. Total deletion of phosphoacceptor sites in the CTE affected proper receptor regulation. However, proximal and distal phosphoacceptor sites both turned out to be sufficient to induce WT-like desensitization. Overexpression of the G(q) coupling-deficient mutant GRK2-D110A suppressed ETA-WT signaling but failed to decrease phospholipase C activity mediated by the phosphorylation-deficient mutant ETA-6PD. In contrast, GRK2-WT acted on both receptors, whereas the kinase-inactive mutant GRK2-D110A/K220R failed to inhibit signaling of ETA-WT and ETA-6PD. This demonstrates that ETA desensitization involves at least two autonomous GRK2-mediated components: 1) a phosphorylation-independent signal decrease mediated by blocking of G(q) and 2) a mechanism involving phosphorylation of Ser and Thr residues in the CTE of the receptor in a redundant fashion, able to incorporate either proximal or distal phosphoacceptor sites. High level transfection of GRK2 variants influenced signaling of ETA-WT and ETA-6PD and hints at an additional phosphorylation-independent regulatory mechanism. Furthermore, internalization of mRuby-tagged receptors was observed with ETA-WT and the phosphorylation-deficient mutant ETA-14PD (lacking 14 phosphoacceptor sites) and turned out to be based on a phosphorylation-independent mechanism.
Stichworte
Desensitization; Receptor; Phosphorylation; Endothelin; G Protein-coupled Receptor (GPCR); Receptor Endocytosis; Receptor Regulation
Erscheinungsjahr
2013
Zeitschriftentitel
Journal of Biological Chemistry
Band
288
Ausgabe
45
Seite(n)
32138-32148
ISSN
0021-9258
eISSN
1083-351X
Page URI
https://pub.uni-bielefeld.de/record/2650926

Zitieren

Gaertner F, Seidel T, Schulz U, Gummert J, Milting H. Desensitization and Internalization of Endothelin Receptor A IMPACT OF G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2)-MEDIATED PHOSPHORYLATION. Journal of Biological Chemistry. 2013;288(45):32138-32148.
Gaertner, F., Seidel, T., Schulz, U., Gummert, J., & Milting, H. (2013). Desensitization and Internalization of Endothelin Receptor A IMPACT OF G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2)-MEDIATED PHOSPHORYLATION. Journal of Biological Chemistry, 288(45), 32138-32148. doi:10.1074/jbc.M113.461566
Gaertner, F., Seidel, T., Schulz, U., Gummert, J., and Milting, H. (2013). Desensitization and Internalization of Endothelin Receptor A IMPACT OF G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2)-MEDIATED PHOSPHORYLATION. Journal of Biological Chemistry 288, 32138-32148.
Gaertner, F., et al., 2013. Desensitization and Internalization of Endothelin Receptor A IMPACT OF G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2)-MEDIATED PHOSPHORYLATION. Journal of Biological Chemistry, 288(45), p 32138-32148.
F. Gaertner, et al., “Desensitization and Internalization of Endothelin Receptor A IMPACT OF G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2)-MEDIATED PHOSPHORYLATION”, Journal of Biological Chemistry, vol. 288, 2013, pp. 32138-32148.
Gaertner, F., Seidel, T., Schulz, U., Gummert, J., Milting, H.: Desensitization and Internalization of Endothelin Receptor A IMPACT OF G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2)-MEDIATED PHOSPHORYLATION. Journal of Biological Chemistry. 288, 32138-32148 (2013).
Gaertner, Florian, Seidel, Thorsten, Schulz, Uwe, Gummert, Jan, and Milting, Hendrik. “Desensitization and Internalization of Endothelin Receptor A IMPACT OF G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2)-MEDIATED PHOSPHORYLATION”. Journal of Biological Chemistry 288.45 (2013): 32138-32148.

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