Identification and characterization of the channel-forming protein in the cell wall of Corynebacterium amycolatum

Soltan Mohammadi N, Mafakheri S, Abdali N, Bárcena-Uribarri I, Tauch A, Benz R (2013)
Biochimica et biophysica acta 1828(11): 2574-2582.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Soltan Mohammadi, Nafiseh; Mafakheri, Samaneh; Abdali, Narges; Bárcena-Uribarri, Iván; Tauch, AndreasUniBi; Benz, Roland
Abstract / Bemerkung
The mycolic-acid layer of certain gram-positive bacteria, the mycolata, represents an additional permeability barrier for the permeation of small water-soluble solutes. Consequently, it was shown in recent years that the mycolic acid layer of individual bacteria of the group mycolata contains pores, called porins, for the passage of hydrophilic solutes. Corynebacterium amycolatum, a pathogenic Corynebacterium species, belongs to the Corynebacteriaceae family but it lacks corynomycolic acids in its cell wall. Despite the absence of corynomycolic acids the cell wall of C. amycolatum contains a cation-selective cell wall channel, which may be responsible for the limited permeability of the cell wall of C. amycolatum. Based on partial sequencing of the protein responsible for channel formation derived from C. amycolatum ATCC 49368 we were able to identify the gene coram0001_1986 within the known genome sequence of C. amycolatum SK46 that codes for the cell wall channel. The corresponding gene of C. amycolatum ATCC 49368 was cloned into the plasmid pXHis for its expression in Corynebacterium glutamicum ∆porA∆porH. Biophysical characterization of the purified protein (PorAcoram) suggested that coram0001_1986 is indeed the gene coding for the pore-forming protein PorAcoram in C. amycolatum ATCC 49368. The protein belongs to the DUF (Domains of Unknown Function) 3068 superfamily of proteins, mainly found in bacteria from the family Corynebacteriaceae. The nearest relative to PorAcoram within this family is an ORF which codes for PorAcres, which was also recognized in reconstitution experiments as a channel-forming protein in Corynebacterium resistens.
Erscheinungsjahr
2013
Zeitschriftentitel
Biochimica et biophysica acta
Band
1828
Ausgabe
11
Seite(n)
2574-2582
ISSN
0005-2736
Page URI
https://pub.uni-bielefeld.de/record/2634035

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Soltan Mohammadi N, Mafakheri S, Abdali N, Bárcena-Uribarri I, Tauch A, Benz R. Identification and characterization of the channel-forming protein in the cell wall of Corynebacterium amycolatum. Biochimica et biophysica acta. 2013;1828(11):2574-2582.
Soltan Mohammadi, N., Mafakheri, S., Abdali, N., Bárcena-Uribarri, I., Tauch, A., & Benz, R. (2013). Identification and characterization of the channel-forming protein in the cell wall of Corynebacterium amycolatum. Biochimica et biophysica acta, 1828(11), 2574-2582. doi:10.1016/j.bbamem.2013.06.024
Soltan Mohammadi, N., Mafakheri, S., Abdali, N., Bárcena-Uribarri, I., Tauch, A., and Benz, R. (2013). Identification and characterization of the channel-forming protein in the cell wall of Corynebacterium amycolatum. Biochimica et biophysica acta 1828, 2574-2582.
Soltan Mohammadi, N., et al., 2013. Identification and characterization of the channel-forming protein in the cell wall of Corynebacterium amycolatum. Biochimica et biophysica acta, 1828(11), p 2574-2582.
N. Soltan Mohammadi, et al., “Identification and characterization of the channel-forming protein in the cell wall of Corynebacterium amycolatum”, Biochimica et biophysica acta, vol. 1828, 2013, pp. 2574-2582.
Soltan Mohammadi, N., Mafakheri, S., Abdali, N., Bárcena-Uribarri, I., Tauch, A., Benz, R.: Identification and characterization of the channel-forming protein in the cell wall of Corynebacterium amycolatum. Biochimica et biophysica acta. 1828, 2574-2582 (2013).
Soltan Mohammadi, Nafiseh, Mafakheri, Samaneh, Abdali, Narges, Bárcena-Uribarri, Iván, Tauch, Andreas, and Benz, Roland. “Identification and characterization of the channel-forming protein in the cell wall of Corynebacterium amycolatum”. Biochimica et biophysica acta 1828.11 (2013): 2574-2582.

8 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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