Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production

Soares SC, Trost E, Ramos RTJ, Carneiro AR, Santos AR, Pinto AC, Barbosa E, Aburjaile F, Ali A, Diniz CAA, Hassan SS, et al. (2013)
Journal of Biotechnology 167(2): 135-141.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Soares, Siomar C.; Trost, EvaUniBi ; Ramos, Rommel T. J.; Carneiro, Adriana R.; Santos, Anderson R.; Pinto, Anne C.; Barbosa, Eudes; Aburjaile, Flávia; Ali, Amjad; Diniz, Carlos A. A.; Hassan, Syed S.; Fiaux, Karina
Abstract / Bemerkung
Corynebacterium pseudotuberculosis is the causative agent of several veterinary diseases in a broad range of economically important hosts, which can vary from caseous lymphadenitis in sheep and goats (biovar ovis) to ulcerative lymphangitis in cattle and horses (biovar equi). Existing vaccines against C. pseudotuberculosis are mainly intended for small ruminants and, even in these hosts, they still present remarkable limitations. In this study, we present the complete genome sequence of C. pseudotuberculosis biovar equi strain 258, isolated from a horse with ulcerative lymphangitis. The genome has a total size of 2,314,404bp and contains 2088 predicted protein-coding regions. Using in silico analysis, eleven pathogenicity islands were detected in the genome sequence of C. pseudotuberculosis 258. The application of a reverse vaccinology strategy identified 49 putative antigenic proteins, which can be used as candidate vaccine targets in future works.
Journal of Biotechnology
Page URI


Soares SC, Trost E, Ramos RTJ, et al. Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production. Journal of Biotechnology. 2013;167(2):135-141.
Soares, S. C., Trost, E., Ramos, R. T. J., Carneiro, A. R., Santos, A. R., Pinto, A. C., Barbosa, E., et al. (2013). Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production. Journal of Biotechnology, 167(2), 135-141. doi:10.1016/j.jbiotec.2012.11.003
Soares, S. C., Trost, E., Ramos, R. T. J., Carneiro, A. R., Santos, A. R., Pinto, A. C., Barbosa, E., Aburjaile, F., Ali, A., Diniz, C. A. A., et al. (2013). Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production. Journal of Biotechnology 167, 135-141.
Soares, S.C., et al., 2013. Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production. Journal of Biotechnology, 167(2), p 135-141.
S.C. Soares, et al., “Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production”, Journal of Biotechnology, vol. 167, 2013, pp. 135-141.
Soares, S.C., Trost, E., Ramos, R.T.J., Carneiro, A.R., Santos, A.R., Pinto, A.C., Barbosa, E., Aburjaile, F., Ali, A., Diniz, C.A.A., Hassan, S.S., Fiaux, K., Guimarães, L.C., Bakhtiar, S.M., Pereira, U., Almeida, S.S., Abreu, V.A.C., Rocha, F.S., Dorella, F.A., Miyoshi, A., Silva, A., Azevedo, V., Tauch, A.: Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production. Journal of Biotechnology. 167, 135-141 (2013).
Soares, Siomar C., Trost, Eva, Ramos, Rommel T. J., Carneiro, Adriana R., Santos, Anderson R., Pinto, Anne C., Barbosa, Eudes, Aburjaile, Flávia, Ali, Amjad, Diniz, Carlos A. A., Hassan, Syed S., Fiaux, Karina, Guimarães, Luis C., Bakhtiar, Syeda M., Pereira, Ulisses, Almeida, Sintia S., Abreu, Vinícius A. C., Rocha, Flávia S., Dorella, Fernanda A., Miyoshi, Anderson, Silva, Artur, Azevedo, Vasco, and Tauch, Andreas. “Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production”. Journal of Biotechnology 167.2 (2013): 135-141.

19 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Putative vaccine candidates and drug targets identified by reverse vaccinology and subtractive genomics approaches to control Haemophilus ducreyi, the causative agent of chancroid.
de Sarom A, Kumar Jaiswal A, Tiwari S, de Castro Oliveira L, Barh D, Azevedo V, Jose Oliveira C, de Castro Soares S., J R Soc Interface 15(142), 2018
PMID: 29792307
Immune-Informatic Analysis and Design of Peptide Vaccine From Multi-epitopes Against Corynebacterium pseudotuberculosis.
Droppa-Almeida D, Franceschi E, Padilha FF., Bioinform Biol Insights 12(), 2018
PMID: 29780242
Rapidly evolving changes and gene loss associated with host switching in Corynebacterium pseudotuberculosis.
Viana MVC, Sahm A, Góes Neto A, Figueiredo HCP, Wattam AR, Azevedo V., PLoS One 13(11), 2018
PMID: 30419061
Complete genome sequence of Vibrio mimicus strain SCCF01 with potential application in fish vaccine development.
Yu Z, Geng Y, Wang K, Chen D, Huang X, Ou Y, Peng G., Virulence 8(6), 2017
PMID: 27763808
Genomic analysis of four strains of Corynebacterium pseudotuberculosis bv. Equi isolated from horses showing distinct signs of infection.
Baraúna RA, Ramos RTJ, Veras AAO, de Sá PHCG, Guimarães LC, das Graças DA, Carneiro AR, Edman JM, Spier SJ, Azevedo V, Silva A., Stand Genomic Sci 12(), 2017
PMID: 28163825
Exploration of Nitrate Reductase Metabolic Pathway in Corynebacterium pseudotuberculosis.
Almeida S, Sousa C, Abreu V, Diniz C, Dorneles EM, Lage AP, Barh D, Azevedo V., Int J Genomics 2017(), 2017
PMID: 28316974
Analyses of the probiotic property and stress resistance-related genes of Lactococcus lactis subsp. lactis NCDO 2118 through comparative genomics and in vitro assays.
Oliveira LC, Saraiva TD, Silva WM, Pereira UP, Campos BC, Benevides LJ, Rocha FS, Figueiredo HC, Azevedo V, Soares SC., PLoS One 12(4), 2017
PMID: 28384209
Whole-Genome Sequence of Corynebacterium pseudotuberculosis PA04, Isolated from the Lymph Node of a Sheep in the Amazon, Brazil.
Costa WLO, Alves JTC, Dias LM, Araújo CLA, Morais E, Silva AGM, Andrade SS, Ramos RTJ, Silva A, Folador ARC., Genome Announc 5(16), 2017
PMID: 28428301
Comparative genomic analysis between Corynebacterium pseudotuberculosis strains isolated from buffalo.
Viana MVC, Figueiredo H, Ramos R, Guimarães LC, Pereira FL, Dorella FA, Selim SAK, Salaheldean M, Silva A, Wattam AR, Azevedo V., PLoS One 12(4), 2017
PMID: 28445543
Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains.
Silva WM, Folador EL, Soares SC, Souza GHMF, Santos AV, Sousa CS, Figueiredo H, Miyoshi A, Le Loir Y, Silva A, Azevedo V., BMC Genomics 18(1), 2017
PMID: 28595597
Quantitative Proteomic Analysis Reveals Changes in the Benchmark Corynebacterium pseudotuberculosis Biovar Equi Exoproteome after Passage in a Murine Host.
Silva WM, Carvalho RDO, Dorella FA, Folador EL, Souza GHMF, Pimenta AMC, Figueiredo HCP, Le Loir Y, Silva A, Azevedo V., Front Cell Infect Microbiol 7(), 2017
PMID: 28791255
Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi.
Almeida S, Dorneles EMS, Diniz C, Abreu V, Sousa C, Alves J, Carneiro A, Bagano P, Spier S, Barh D, Lage AP, Figueiredo H, Azevedo V., BMC Vet Res 13(1), 2017
PMID: 28946887
Recombinant CP40 from Corynebacterium pseudotuberculosis confers protection in mice after challenge with a virulent strain.
Droppa-Almeida D, Vivas WL, Silva KK, Rezende AF, Simionatto S, Meyer R, Lima-Verde IB, Delagostin O, Borsuk S, Padilha FF., Vaccine 34(8), 2016
PMID: 26796140
SIMBA: a web tool for managing bacterial genome assembly generated by Ion PGM sequencing technology.
Mariano DC, Pereira FL, Aguiar EL, Oliveira LC, Benevides L, Guimarães LC, Folador EL, Sousa TJ, Ghosh P, Barh D, Figueiredo HC, Silva A, Ramos RT, Azevedo VA., BMC Bioinformatics 17(suppl 18), 2016
PMID: 28105921
Corynebacterium pseudotuberculosis Pneumonia in a Veterinary Student Infected During Laboratory Work.
Heggelund L, Gaustad P, Håvelsrud OE, Blom J, Borgen L, Sundset A, Sørum H, Frøland SS., Open Forum Infect Dis 2(2), 2015
PMID: 26380345
An integrated structural proteomics approach along the druggable genome of Corynebacterium pseudotuberculosis species for putative druggable targets.
Radusky LG, Hassan S, Lanzarotti E, Tiwari S, Jamal S, Ali J, Ali A, Ferreira R, Barh D, Silva A, Turjanski AG, Azevedo VA., BMC Genomics 16 Suppl 5(), 2015
PMID: 26041381
Genome Sequence of Corynebacterium pseudotuberculosis MB20 bv. equi Isolated from a Pectoral Abscess of an Oldenburg Horse in California.
Baraúna RA, Guimarães LC, Veras AA, de Sá PH, Graças DA, Pinheiro KC, Silva AS, Folador EL, Benevides LJ, Viana MV, Carneiro AR, Schneider MP, Spier SJ, Edman JM, Ramos RT, Azevedo V, Silva A., Genome Announc 2(6), 2014
PMID: 25395628

56 References

Daten bereitgestellt von Europe PubMed Central.

Corynebacterium pseudotuberculosis infection in horses: 538 cases (1982-1993).
Aleman M, Spier SJ, Wilson WD, Doherr M., J. Am. Vet. Med. Assoc. 209(4), 1996
PMID: 8756884
BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons.
Alikhan NF, Petty NK, Ben Zakour NL, Beatson SA., BMC Genomics 12(), 2011
PMID: 21824423
Overcoming the heme paradox: heme toxicity and tolerance in bacterial pathogens.
Anzaldi LL, Skaar EP., Infect. Immun. 78(12), 2010
PMID: 20679437
Prevalence of and carcass condemnation from maedi-visna, paratuberculosis and caseous lymphadenitis in culled sheep from Quebec, Canada.
Arsenault J, Girard C, Dubreuil P, Daignault D, Galarneau JR, Boisclair J, Simard C, Belanger D., Prev. Vet. Med. 59(1-2), 2003
PMID: 12719018
Two serotypes of Corynebacterium pseudotuberculosis isolated from different animal species
Barakat, Revue Scientifique et Technique de l’OIE 3(), 1984
A novel comparative genomics analysis for common drug and vaccine targets in Corynebacterium pseudotuberculosis and other CMN group of human pathogens.
Barh D, Jain N, Tiwari S, Parida BP, D'Afonseca V, Li L, Ali A, Santos AR, Guimaraes LC, de Castro Soares S, Miyoshi A, Bhattacharjee A, Misra AN, Silva A, Kumar A, Azevedo V., Chem Biol Drug Des 78(1), 2011
PMID: 21443692
Prediction of surface exposed proteins in Streptococcus pyogenes, with a potential application to other Gram-positive bacteria.
Barinov A, Loux V, Hammani A, Nicolas P, Langella P, Ehrlich D, Maguin E, van de Guchte M., Proteomics 9(1), 2009
PMID: 19053137
Two biotypes of Corynebacterium pseudotuberculosis.
Biberstein EL, Knight HD, Jang S., Vet. Rec. 89(26), 1971
PMID: 5168555
Effect of muramyl dipeptide on immunogenicity of Corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs.
Brogden KA, Chedid L, Cutlip RC, Lehmkuhl HD, Sacks J., Am. J. Vet. Res. 51(2), 1990
PMID: 2301830
ACT: the Artemis comparison tool
Carver, Bioinformatics 16(), 2005
Rapid hybrid de novo assembly of a microbial genome using only short reads: Corynebacterium pseudotuberculosis I19 as a case study.
Cerdeira LT, Carneiro AR, Ramos RT, de Almeida SS, D'Afonseca V, Schneider MP, Baumbach J, Tauch A, McCulloch JA, Azevedo VA, Silva A., J. Microbiol. Methods 86(2), 2011
PMID: 21620904
Complete genome sequence of Corynebacterium pseudotuberculosis strain CIP 52.97, isolated from a horse in Kenya.
Cerdeira LT, Schneider MP, Pinto AC, de Almeida SS, dos Santos AR, Barbosa EG, Ali A, Aburjaile FF, de Abreu VA, Guimaraes LC, Soares Sde C, Dorella FA, Rocha FS, Bol E, Gomes de Sa PH, Lopes TS, Barbosa MS, Carneiro AR, Juca Ramos RT, Coimbra NA, Lima AR, Barh D, Jain N, Tiwari S, Raja R, Zambare V, Ghosh P, Trost E, Tauch A, Miyoshi A, Azevedo V, Silva A., J. Bacteriol. 193(24), 2011
PMID: 22123771
The structure of the ferric siderophore binding protein FhuD complexed with gallichrome
Clarke, Natural Structural Biology 7(), 2000
Antigens of Corynebacterium pseudotuberculosis and prospects for vaccine development.
Dorella FA, Pacheco LG, Seyffert N, Portela RW, Meyer R, Miyoshi A, Azevedo V., Expert Rev Vaccines 8(2), 2009
PMID: 19196200
Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence.
Dorella FA, Pacheco LG, Oliveira SC, Miyoshi A, Azevedo V., Vet. Res. 37(2), 2006
PMID: 16472520
Penicillin-binding proteins in bacteria.
Georgopapadakou NH, Liu FY., Antimicrob. Agents Chemother. 18(1), 1980
PMID: 7416741
Physiological functions of D-alanine carboxypeptidases in Escherichia coli.
Ghosh AS, Chowdhury C, Nelson DE., Trends Microbiol. 16(7), 2008
PMID: 18539032
Vaxign: the first web-based vaccine design program for reverse vaccinology and applications for vaccine development
He, Journal of Biomedicine and Biotechnology 2010(), 2010
De novo bacterial genome sequencing: millions of very short reads assembled on a desktop computer.
Hernandez D, Francois P, Farinelli L, Osteras M, Schrenzel J., Genome Res. 18(5), 2008
PMID: 18332092
Efficacy of an ovine caseous lymphadenitis vaccine formulated using a genetically inactive form of the Corynebacterium pseudotuberculosis phospholipase D.
Hodgson AL, Carter K, Tachedjian M, Krywult J, Corner LA, McColl M, Cameron A., Vaccine 17(7-8), 1999
PMID: 10067685

Jones, 1986
RNAmmer: consistent and rapid annotation of ribosomal RNA genes.
Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW., Nucleic Acids Res. 35(9), 2007
PMID: 17452365
Host cell adhesion to Schistosoma mansoni larvae in the peritoneal cavity of naive mice. Histological and scanning electron microscopic studies
Melo, Revista do Instituto de Medicina Tropical de São Paulo 35(1), 1993
Prevalence of caseous lymphadenitis and usage of caseous lymphadenitis vaccines in sheep flocks.
Paton MW, Walker SB, Rose IR, Watt GF., Aust. Vet. J. 81(1-2), 2003
PMID: 15084020
The ABCDs of periplasmic copper trafficking.
Puig S, Rees EM, Thiele DJ., Structure 10(10), 2002
PMID: 12377116
Analysis of quality raw data of second generation sequencers with Quality Assessment Software.
Ramos RT, Carneiro AR, Baumbach J, Azevedo V, Schneider MP, Silva A., BMC Res Notes 4(), 2011
PMID: 21501521
Potential of Mycobacterium tuberculosis resuscitation-promoting factors as antigens in novel tuberculosis sub-unit vaccines.
Romano M, Aryan E, Korf H, Bruffaerts N, Franken CL, Ottenhoff TH, Huygen K., Microbes Infect. 14(1), 2011
PMID: 21920450
Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
Ruiz JC, D'Afonseca V, Silva A, Ali A, Pinto AC, Santos AR, Rocha AA, Lopes DO, Dorella FA, Pacheco LG, Costa MP, Turk MZ, Seyffert N, Moraes PM, Soares SC, Almeida SS, Castro TL, Abreu VA, Trost E, Baumbach J, Tauch A, Schneider MP, McCulloch J, Cerdeira LT, Ramos RT, Zerlotini A, Dominitini A, Resende DM, Coser EM, Oliveira LM, Pedrosa AL, Vieira CU, Guimaraes CT, Bartholomeu DC, Oliveira DM, Santos FR, Rabelo EM, Lobo FP, Franco GR, Costa AF, Castro IM, Dias SR, Ferro JA, Ortega JM, Paiva LV, Goulart LR, Almeida JF, Ferro MI, Carneiro NP, Falcao PR, Grynberg P, Teixeira SM, Brommonschenkel S, Oliveira SC, Meyer R, Moore RJ, Miyoshi A, Oliveira GC, Azevedo V., PLoS ONE 6(4), 2011
PMID: 21533164
Artemis: sequence visualization and annotation.
Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA, Barrell B., Bioinformatics 16(10), 2000
PMID: 11120685
Phospholipase D: enzymology, functionality, and chemical modulation.
Selvy PE, Lavieri RR, Lindsley CW, Brown HA., Chem. Rev. 111(10), 2011
PMID: 21936578
MalE of group A Streptococcus participates in the rapid transport of maltotriose and longer maltodextrins.
Shelburne SA 3rd, Fang H, Okorafor N, Sumby P, Sitkiewicz I, Keith D, Patel P, Austin C, Graviss EA, Musser JM, Chow DC., J. Bacteriol. 189(7), 2007
PMID: 17259319
PIPS: pathogenicity island prediction software.
Soares SC, Abreu VA, Ramos RT, Cerdeira L, Silva A, Baumbach J, Trost E, Tauch A, Hirata R Jr, Mattos-Guaraldi AL, Miyoshi A, Azevedo V., PLoS ONE 7(2), 2012
PMID: 22355329
Ulcerative lymphangitis and coronet lesions in an Israeli dairy herd infected with Corynebacterium pseudotuberculosis
Steinman, Veterinary Record 145(), 1999
Comparative analysis of two complete Corynebacterium ulcerans genomes and detection of candidate virulence factors.
Trost E, Al-Dilaimi A, Papavasiliou P, Schneider J, Viehoever P, Burkovski A, Soares SC, Almeida SS, Dorella FA, Miyoshi A, Azevedo V, Schneider MP, Silva A, Santos CS, Santos LS, Sabbadini P, Dias AA, Hirata R Jr, Mattos-Guaraldi AL, Tauch A., BMC Genomics 12(), 2011
PMID: 21801446
The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence.
Trost E, Ott L, Schneider J, Schroder J, Jaenicke S, Goesmann A, Husemann P, Stoye J, Dorella FA, Rocha FS, Soares Sde C, D'Afonseca V, Miyoshi A, Ruiz J, Silva A, Azevedo V, Burkovski A, Guiso N, Join-Lambert OF, Kayal S, Tauch A., BMC Genomics 11(), 2010
PMID: 21192786
Deletion of the Mycobacterium tuberculosis resuscitation-promoting factor Rv1009 gene results in delayed reactivation from chronic tuberculosis.
Tufariello JM, Mi K, Xu J, Manabe YC, Kesavan AK, Drumm J, Tanaka K, Jacobs WR Jr, Chan J., Infect. Immun. 74(5), 2006
PMID: 16622237
Caseous lymphadenitis in small ruminants
Williamson, Veterinary Clinics of North America: Food Animal Practice 17(), 2001
Control of caseous lymphadenitis
Windsor, Veterinary Clinics of North America: Food Animal Practice 27(), 2011
Corynebacterium pseudotuberculosis infection in Israeli dairy cattle.
Yeruham I, Elad D, Friedman S, Perl S., Epidemiol. Infect. 131(2), 2003
PMID: 14596537
A herd level analysis of a Corynebacterium pseudotuberculosis outbreak in a dairy cattle herd.
Yeruham I, Friedman S, Perl S, Elad D, Berkovich Y, Kalgard Y., Vet. Dermatol. 15(5), 2004
PMID: 15500484
Velvet: algorithms for de novo short read assembly using de Bruijn graphs.
Zerbino DR, Birney E., Genome Res. 18(5), 2008
PMID: 18349386


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 23201561
PubMed | Europe PMC

Suchen in

Google Scholar