Membrane and actin reorganization in electropulse-induced cell fusion

Gerisch G, Ecke M, Neujahr R, Prassler J, Stengl A, Hoffmann M, Schwarz US, Neumann E (2013)
Journal Of Cell Science 126(9): 2069-2078.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
When cells of Dictyostelium discoideum are exposed to electric pulses they are induced to fuse, yielding motile polykaryotic cells. By combining electron microscopy and direct recording of fluorescent cells, we have studied the emergence of fusion pores in the membranes and the localization of actin to the cell cortex. In response to electric pulsing, the plasma membranes of two contiguous cells are turned into tangles of highly bent and interdigitated membranes. Live-imaging of cells double-labeled for membranes and filamentous actin revealed that actin is induced to polymerize in the fusion zone to temporarily bridge the gaps in the vesiculating membrane. The diffusion of green fluorescent protein (GFP) from one fusion partner to the other was scored using spinning disc confocal microscopy. Fusion pores that allowed intercellular exchange of GFP were formed after a delay, which lasted up to 24 seconds after exposure of the cells to the electric field. These data indicate that the membranes persist in a fusogenic state before pores of about 3 nm diameter are formed.
Stichworte
Electric lipid distortion; Diffusion; Actin polymerization; Cell repair; Fusion pores; Membrane fusion
Erscheinungsjahr
2013
Zeitschriftentitel
Journal Of Cell Science
Band
126
Ausgabe
9
Seite(n)
2069-2078
ISSN
0021-9533
eISSN
1477-9137
Page URI
https://pub.uni-bielefeld.de/record/2613031

Zitieren

Gerisch G, Ecke M, Neujahr R, et al. Membrane and actin reorganization in electropulse-induced cell fusion. Journal Of Cell Science. 2013;126(9):2069-2078.
Gerisch, G., Ecke, M., Neujahr, R., Prassler, J., Stengl, A., Hoffmann, M., Schwarz, U. S., et al. (2013). Membrane and actin reorganization in electropulse-induced cell fusion. Journal Of Cell Science, 126(9), 2069-2078. doi:10.1242/jcs.124073
Gerisch, G., Ecke, M., Neujahr, R., Prassler, J., Stengl, A., Hoffmann, M., Schwarz, U. S., and Neumann, E. (2013). Membrane and actin reorganization in electropulse-induced cell fusion. Journal Of Cell Science 126, 2069-2078.
Gerisch, G., et al., 2013. Membrane and actin reorganization in electropulse-induced cell fusion. Journal Of Cell Science, 126(9), p 2069-2078.
G. Gerisch, et al., “Membrane and actin reorganization in electropulse-induced cell fusion”, Journal Of Cell Science, vol. 126, 2013, pp. 2069-2078.
Gerisch, G., Ecke, M., Neujahr, R., Prassler, J., Stengl, A., Hoffmann, M., Schwarz, U.S., Neumann, E.: Membrane and actin reorganization in electropulse-induced cell fusion. Journal Of Cell Science. 126, 2069-2078 (2013).
Gerisch, Guenther, Ecke, Mary, Neujahr, Ralph, Prassler, Jana, Stengl, Andreas, Hoffmann, Max, Schwarz, Ulrich S., and Neumann, Eberhard. “Membrane and actin reorganization in electropulse-induced cell fusion”. Journal Of Cell Science 126.9 (2013): 2069-2078.

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PMID: 23447671
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