Analysis of DNA interactions using single-molecule force spectroscopy

Ritzefeld M, Walhorn V, Anselmetti D, Sewald N (2013)
Amino acids 44(6): 1457-1475.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Abstract / Bemerkung
Protein-DNA interactions are involved in many biochemical pathways and determine the fate of the corresponding cell. Qualitative and quantitative investigations on these recognition and binding processes are of key importance for an improved understanding of biochemical processes and also for systems biology. This review article focusses on atomic force microscopy (AFM)-based single-molecule force spectroscopy and its application to the quantification of forces and binding mechanisms that lead to the formation of protein-DNA complexes. AFM and dynamic force spectroscopy are exciting tools that allow for quantitative analysis of biomolecular interactions. Besides an overview on the method and the most important immobilization approaches, the physical basics of the data evaluation is described. Recent applications of AFM-based force spectroscopy to investigate DNA intercalation, complexes involving DNA aptamers and peptide- and protein-DNA interactions are given.
Erscheinungsjahr
2013
Zeitschriftentitel
Amino acids
Band
44
Ausgabe
6
Seite(n)
1457-1475
ISSN
0939-4451
eISSN
1438-2199
Page URI
https://pub.uni-bielefeld.de/record/2584415

Zitieren

Ritzefeld M, Walhorn V, Anselmetti D, Sewald N. Analysis of DNA interactions using single-molecule force spectroscopy. Amino acids. 2013;44(6):1457-1475.
Ritzefeld, M., Walhorn, V., Anselmetti, D., & Sewald, N. (2013). Analysis of DNA interactions using single-molecule force spectroscopy. Amino acids, 44(6), 1457-1475. doi:10.1007/s00726-013-1474-4
Ritzefeld, M., Walhorn, V., Anselmetti, D., and Sewald, N. (2013). Analysis of DNA interactions using single-molecule force spectroscopy. Amino acids 44, 1457-1475.
Ritzefeld, M., et al., 2013. Analysis of DNA interactions using single-molecule force spectroscopy. Amino acids, 44(6), p 1457-1475.
M. Ritzefeld, et al., “Analysis of DNA interactions using single-molecule force spectroscopy”, Amino acids, vol. 44, 2013, pp. 1457-1475.
Ritzefeld, M., Walhorn, V., Anselmetti, D., Sewald, N.: Analysis of DNA interactions using single-molecule force spectroscopy. Amino acids. 44, 1457-1475 (2013).
Ritzefeld, Markus, Walhorn, Volker, Anselmetti, Dario, and Sewald, Norbert. “Analysis of DNA interactions using single-molecule force spectroscopy”. Amino acids 44.6 (2013): 1457-1475.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 23468137
PubMed | Europe PMC

Suchen in

Google Scholar