The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import

Fahrenkrog B, Hübner W, Mandinova A, Panté N, Keller W, Aebi U (2000)
Molecular biology of the cell 11(11): 3885-3896.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Fahrenkrog, B; Hübner, WolfgangUniBi ; Mandinova, A; Panté, N; Keller, W; Aebi, U
Einrichtung
Fakultät für Physik > AG Biomolekulare Photonik
Abstract / Bemerkung
The bidirectional nucleocytoplasmic transport of macromolecules is mediated by the nuclear pore complex (NPC) which, in yeast, is composed of approximately 30 different proteins (nucleoporins). Pre-embedding immunogold-electron microscopy revealed that Nic96p, an essential yeast nucleoporin, is located about the cytoplasmic and the nuclear periphery of the central channel, and near or at the distal ring of the yeast NPC. Genetic approaches further implicated Nic96p in nuclear protein import. To more specifically explore the potential role of Nic96p in nuclear protein import, we performed a two-hybrid screen with NIC96 as the bait against a yeast genomic library to identify transport factors and/or nucleoporins involved in nuclear protein import interacting with Nic96p. By doing so, we identified the yeast nucleoporin Nup53p, which also exhibits multiple locations within the yeast NPC and colocalizes with Nic96p in all its locations. Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export.
Stichworte
Nuclear Proteins/genetics; Nuclear Pore Complex Proteins; Nuclear Localization Signals; Mutation; Immunoelectron; Microscopy; Membrane Proteins; Gene Deletion; Fungal Proteins/metabolism; Fungal Proteins/genetics; Cell Nucleus/ultrastructure; Cell Nucleus; Active Transport; Cell Nucleus/metabolism; Nuclear Proteins/metabolism; Porins/genetics; Porins/metabolism; Saccharomyces cerevisiae Proteins; Two-Hybrid System Techniques; Yeasts/genetics; Yeasts/metabolism; beta Karyopherins
Erscheinungsjahr
2000
Zeitschriftentitel
Molecular biology of the cell
Band
11
Ausgabe
11
Seite(n)
3885-3896
ISSN
1059-1524
Page URI
https://pub.uni-bielefeld.de/record/2577057

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Fahrenkrog B, Hübner W, Mandinova A, Panté N, Keller W, Aebi U. The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import. Molecular biology of the cell. 2000;11(11):3885-3896.
Fahrenkrog, B., Hübner, W., Mandinova, A., Panté, N., Keller, W., & Aebi, U. (2000). The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import. Molecular biology of the cell, 11(11), 3885-3896. doi:10.1091/mbc.11.11.3885
Fahrenkrog, B, Hübner, Wolfgang, Mandinova, A, Panté, N, Keller, W, and Aebi, U. 2000. “The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import”. Molecular biology of the cell 11 (11): 3885-3896.
Fahrenkrog, B., Hübner, W., Mandinova, A., Panté, N., Keller, W., and Aebi, U. (2000). The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import. Molecular biology of the cell 11, 3885-3896.
Fahrenkrog, B., et al., 2000. The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import. Molecular biology of the cell, 11(11), p 3885-3896.
B. Fahrenkrog, et al., “The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import”, Molecular biology of the cell, vol. 11, 2000, pp. 3885-3896.
Fahrenkrog, B., Hübner, W., Mandinova, A., Panté, N., Keller, W., Aebi, U.: The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import. Molecular biology of the cell. 11, 3885-3896 (2000).
Fahrenkrog, B, Hübner, Wolfgang, Mandinova, A, Panté, N, Keller, W, and Aebi, U. “The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import”. Molecular biology of the cell 11.11 (2000): 3885-3896.

16 Zitationen in Europe PMC

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