Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination

Dichtl B, Blank D, Sadowski M, Hübner W, Weiser S, Keller W (2002)
The EMBO journal 21(15): 4125-4135.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Dichtl, Bernhard; Blank, Diana; Sadowski, Martin; Hübner, WolfgangUniBi ; Weiser, Stefan; Keller, Walter
Abstract / Bemerkung
RNA polymerase II (pol II) transcription termination requires co-transcriptional recognition of a functional polyadenylation signal, but the molecular mechanisms that transduce this signal to pol II remain unclear. We show that Yhh1p/Cft1p, the yeast homologue of the mammalian AAUAAA interacting protein CPSF 160, is an RNA-binding protein and provide evidence that it participates in poly(A) site recognition. Interestingly, RNA binding is mediated by a central domain composed of predicted beta-propeller-forming repeats, which occurs in proteins of diverse cellular functions. We also found that Yhh1p/Cft1p bound specifically to the phosphorylated C-terminal domain (CTD) of pol II in vitro and in a two-hybrid test in vivo. Furthermore, transcriptional run-on analysis demonstrated that yhh1 mutants were defective in transcription termination, suggesting that Yhh1p/Cft1p functions in the coupling of transcription and 3'-end formation. We propose that direct interactions of Yhh1p/Cft1p with both the RNA transcript and the CTD are required to communicate poly(A) site recognition to elongating pol II to initiate transcription termination.
Recombinant Fusion Proteins/physiology; RNA-Binding Proteins/physiology; RNA-Binding Proteins/genetics; RNA-Binding Proteins/chemistry; RNA; Messenger/metabolism; Fungal/metabolism; RNA; RNA Polymerase II/physiology; Tertiary; Protein Structure; Poly A/metabolism; Translational/physiology; Peptide Chain Termination; Translational; Peptide Chain Elongation; Molecular Sequence Data; Macromolecular Substances; Escherichia coli; Binding Sites; Actins; Amino Acid Sequence; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/physiology; Sequence Alignment; Sequence Homology; Amino Acid; Transcription; Genetic; Two-Hybrid System Techniques; mRNA Cleavage and Polyadenylation Factors
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Dichtl B, Blank D, Sadowski M, Hübner W, Weiser S, Keller W. Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. The EMBO journal. 2002;21(15):4125-4135.
Dichtl, B., Blank, D., Sadowski, M., Hübner, W., Weiser, S., & Keller, W. (2002). Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. The EMBO journal, 21(15), 4125-4135. doi:10.1093/emboj/cdf390
Dichtl, Bernhard, Blank, Diana, Sadowski, Martin, Hübner, Wolfgang, Weiser, Stefan, and Keller, Walter. 2002. “Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination”. The EMBO journal 21 (15): 4125-4135.
Dichtl, B., Blank, D., Sadowski, M., Hübner, W., Weiser, S., and Keller, W. (2002). Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. The EMBO journal 21, 4125-4135.
Dichtl, B., et al., 2002. Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. The EMBO journal, 21(15), p 4125-4135.
B. Dichtl, et al., “Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination”, The EMBO journal, vol. 21, 2002, pp. 4125-4135.
Dichtl, B., Blank, D., Sadowski, M., Hübner, W., Weiser, S., Keller, W.: Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. The EMBO journal. 21, 4125-4135 (2002).
Dichtl, Bernhard, Blank, Diana, Sadowski, Martin, Hübner, Wolfgang, Weiser, Stefan, and Keller, Walter. “Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination”. The EMBO journal 21.15 (2002): 4125-4135.

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