Inhibition of viral assembly in murine cells by HIV-1 matrix

Hübner W, Chen BK (2006)
Virology 352(1): 27-38.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Hübner, WolfgangUniBi ; Chen, Benjamin K
Abstract / Bemerkung
In human cells, the N-terminal matrix (MA) domain of the human immunodeficiency virus type 1 (HIV-1) Gag targets assembly to specific membrane compartments. In murine fibroblasts, membrane targeting of Gag and assembly of HIV-1 are inefficient. These deficiencies are relieved by replacement of HIV-1 MA with murine leukemia virus (MLV) MA in chimeric proviruses. In this study, we examined chimeric HIV-1 carrying tandem MLV and HIV-1 MA domains and found that the addition of MLV MA to the N-terminus of HIV-1 Gag enhanced membrane binding in murine cells, but was not sufficient to stimulate virus production. Removal of HIV MA was required to observe more efficient Gag processing and increased virus production in murine cells. Deletion of the globular head of MA also alleviated the blocks to membrane binding and Gag processing in murine cells, yet did not lead to increased virus production. These MA-dependent, cell-type-specific phenotypes suggest that host factors interact with the globular head of MA to regulate membrane binding and additional membrane-independent step(s) required for assembly.
Murine/genetics; Leukemia Virus; Humans; HIV-1/pathogenicity; HIV-1/metabolism; HIV-1/genetics; HIV-1/chemistry; HIV Antigens/physiology; HIV Antigens/genetics; HIV Antigens/chemistry; gag/physiology; Gene Products; gag/genetics; Gene Products; gag/chemistry; Gene Products; Viral; Gene Expression Regulation; Gene Deletion; Chimera; Cell Membrane/virology; Cell Membrane/metabolism; Cell Line; 3T3 Cells; Animals; Leukemia Virus; Murine/metabolism; Mice; Viral Matrix Proteins/physiology; Viral Proteins/chemistry; Viral Proteins/genetics; Viral Proteins/physiology; Virus Assembly/drug effects; gag Gene Products; Human Immunodeficiency Virus
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Hübner W, Chen BK. Inhibition of viral assembly in murine cells by HIV-1 matrix. Virology. 2006;352(1):27-38.
Hübner, W., & Chen, B. K. (2006). Inhibition of viral assembly in murine cells by HIV-1 matrix. Virology, 352(1), 27-38. doi:10.1016/j.virol.2006.04.024
Hübner, W., and Chen, B. K. (2006). Inhibition of viral assembly in murine cells by HIV-1 matrix. Virology 352, 27-38.
Hübner, W., & Chen, B.K., 2006. Inhibition of viral assembly in murine cells by HIV-1 matrix. Virology, 352(1), p 27-38.
W. Hübner and B.K. Chen, “Inhibition of viral assembly in murine cells by HIV-1 matrix”, Virology, vol. 352, 2006, pp. 27-38.
Hübner, W., Chen, B.K.: Inhibition of viral assembly in murine cells by HIV-1 matrix. Virology. 352, 27-38 (2006).
Hübner, Wolfgang, and Chen, Benjamin K. “Inhibition of viral assembly in murine cells by HIV-1 matrix”. Virology 352.1 (2006): 27-38.

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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