Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1

Hübner W, Chen P, Del Portillo A, Liu Y, Gordon RE, Chen BK (2007)
Journal of virology 81(22): 12596-12607.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
The assembly of infectious human immunodeficiency virus (HIV) requires that Gag transport and oligomerization be coordinated with its association with other viral proteins, viral RNAs, and cellular membranes. We have developed a replication-competent HIV type 1 molecular clone that carries a Gag-internal or interdomain green fluorescent protein (iGFP) fusion to reveal a physiologically accurate temporal sequence of Gag localization and oligomerization during the formation of infectious HIV. This recombinant HIV is as infectious as native HIV in single-round infectivity assays, validating its use for trafficking studies. It replicates robustly in permissive MT4 cells and is infectious, yet it spreads poorly in other T-cell lines. Immunofluorescence of Gag-iGFP showed a pattern very similar to that of native Gag. However, the intense plasma membrane Gag-iGFP fluorescence contrasts markedly with its immunofluorescence at this site, indicating that many Gag epitopes can be masked by oligomerization. Consistent with this, fluorescence resonance energy transfer studies visualized intense Gag oligomerization at the plasma membrane and weaker oligomerization at cytoplasmic sites. Four-dimensional, time-lapse confocal imaging reveals a temporal progression of Gag distribution over hours in which Gag is initially diffusely localized within the cytoplasm. Plasma membrane signals then accumulate as Gag levels increase and vesicular association appears late, only after plasma membrane site signals have reached high intensity. Lastly, the cell rounds up and HIV protease activation induces diffuse fluorescence throughout the cell. These distinct phases reveal a natural progression of Gag trafficking during the viral gene expression program. HIV Gag-iGFP is a useful tool for dissecting mechanisms of viral assembly and transmission.
Erscheinungsjahr
Zeitschriftentitel
Journal of virology
Band
81
Ausgabe
22
Seite(n)
12596-12607
ISSN
PUB-ID

Zitieren

Hübner W, Chen P, Del Portillo A, Liu Y, Gordon RE, Chen BK. Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1. Journal of virology. 2007;81(22):12596-12607.
Hübner, W., Chen, P., Del Portillo, A., Liu, Y., Gordon, R. E., & Chen, B. K. (2007). Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1. Journal of virology, 81(22), 12596-12607. doi:10.1128/JVI.01088-07
Hübner, W., Chen, P., Del Portillo, A., Liu, Y., Gordon, R. E., and Chen, B. K. (2007). Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1. Journal of virology 81, 12596-12607.
Hübner, W., et al., 2007. Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1. Journal of virology, 81(22), p 12596-12607.
W. Hübner, et al., “Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1”, Journal of virology, vol. 81, 2007, pp. 12596-12607.
Hübner, W., Chen, P., Del Portillo, A., Liu, Y., Gordon, R.E., Chen, B.K.: Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1. Journal of virology. 81, 12596-12607 (2007).
Hübner, Wolfgang, Chen, Ping, Del Portillo, Armando, Liu, Yuxin, Gordon, Ronald E, and Chen, Benjamin K. “Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1”. Journal of virology 81.22 (2007): 12596-12607.

97 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Sequential trafficking of Env and Gag to HIV-1 T cell virological synapses revealed by live imaging.
Wang L, Izadmehr S, Kamau E, Kong XP, Chen BK., Retrovirology 16(1), 2019
PMID: 30646921
Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy.
Inamdar K, Floderer C, Favard C, Muriaux D., Viruses 11(1), 2019
PMID: 30654596
HIV-1 Infection of Primary CD4+ T Cells Regulates the Expression of Specific Human Endogenous Retrovirus HERV-K (HML-2) Elements.
Young GR, Terry SN, Manganaro L, Cuesta-Dominguez A, Deikus G, Bernal-Rubio D, Campisi L, Fernandez-Sesma A, Sebra R, Simon V, Mulder LCF., J Virol 92(1), 2018
PMID: 29046457
Live-Cell Imaging of Early Steps of Single HIV-1 Infection.
Francis AC, Melikyan GB., Viruses 10(5), 2018
PMID: 29783762
NK-cell activation is associated with increased HIV transcriptional activity following allogeneic hematopoietic cell transplantation.
Hogan LE, Körner C, Hobbs K, Simoneau CR, Thanh C, Gibson EA, Palmer CD, Pandit A, Marty FM, Kuritzkes DR, Jost S, Ritz J, Henrich TJ., Blood Adv 2(12), 2018
PMID: 29921650
Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis.
Márquez CL, Lau D, Walsh J, Shah V, McGuinness C, Wong A, Aggarwal A, Parker MW, Jacques DA, Turville S, Böcking T., Elife 7(), 2018
PMID: 29877795
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion.
Esposito AM, Soare AY, Patel F, Satija N, Chen BK, Swartz TH., J Vis Exp (138), 2018
PMID: 30176017
Interferon-inducible LY6E Protein Promotes HIV-1 Infection.
Yu J, Liang C, Liu SL., J Biol Chem 292(11), 2017
PMID: 28130445
Controlling Multicycle Replication of Live-Attenuated HIV-1 Using an Unnatural Genetic Switch.
Yuan Z, Wang N, Kang G, Niu W, Li Q, Guo J., ACS Synth Biol 6(4), 2017
PMID: 28106981
Early cytoplasmic uncoating is associated with infectivity of HIV-1.
Mamede JI, Cianci GC, Anderson MR, Hope TJ., Proc Natl Acad Sci U S A 114(34), 2017
PMID: 28784755
FRET analysis of HIV-1 Gag and GagPol interactions.
Takagi S, Momose F, Morikawa Y., FEBS Open Bio 7(11), 2017
PMID: 29123989
A high throughput Cre-lox activated viral membrane fusion assay identifies pharmacological inhibitors of HIV entry.
Esposito AM, Cheung P, Swartz TH, Li H, Tsibane T, Durham ND, Basler CF, Felsenfeld DP, Chen BK., Virology 490(), 2016
PMID: 26803470
TREX1 Knockdown Induces an Interferon Response to HIV that Delays Viral Infection in Humanized Mice.
Wheeler LA, Trifonova RT, Vrbanac V, Barteneva NS, Liu X, Bollman B, Onofrey L, Mulik S, Ranjbar S, Luster AD, Tager AM, Lieberman J., Cell Rep 15(8), 2016
PMID: 27184854
Labeling of virus components for advanced, quantitative imaging analyses.
Sakin V, Paci G, Lemke EA, Müller B., FEBS Lett 590(13), 2016
PMID: 26987299
Time-Resolved Imaging of Single HIV-1 Uncoating In Vitro and in Living Cells.
Francis AC, Marin M, Shi J, Aiken C, Melikyan GB., PLoS Pathog 12(6), 2016
PMID: 27322072
Optical manipulation of a single human virus for study of viral-cell interactions.
Hou X, DeSantis MC, Tian C, Cheng W., Proc SPIE Int Soc Opt Eng 9922(), 2016
PMID: 27746582
Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling.
Mekdad HE, Boutant E, Karnib H, Biedma ME, Sharma KK, Malytska I, Laumond G, Roy M, Réal E, Paillart JC, Moog C, Darlix JL, Mély Y, de Rocquigny H., Retrovirology 13(1), 2016
PMID: 27515235
Access of HIV-2 to CD169-dependent dendritic cell-mediated trans infection pathway is attenuated.
Kijewski SDG, Akiyama H, Feizpour A, Miller CM, Ramirez NP, Reinhard BM, Gummuluru S., Virology 497(), 2016
PMID: 27521724
M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner.
Wang Q, Zhang X, Han Y, Wang X, Gao G., Sci Rep 6(), 2016
PMID: 27604950
Where in the Cell Are You? Probing HIV-1 Host Interactions through Advanced Imaging Techniques.
Dirk BS, Van Nynatten LR, Dikeakos JD., Viruses 8(10), 2016
PMID: 27775563
Purinergic Receptors: Key Mediators of HIV-1 Infection and Inflammation.
Swartz TH, Dubyak GR, Chen BK., Front Immunol 6(), 2015
PMID: 26635799
A novel modified peptide derived from membrane-proximal external region of human immunodeficiency virus type 1 envelope significantly enhances retrovirus infection.
Zhang L, Jiang C, Zhang H, Gong X, Yang L, Miao L, Shi Y, Zhang Y, Kong W, Zhang C, Shan Y., J Pept Sci 20(1), 2014
PMID: 24254845
Interrelationship between cytoplasmic retroviral Gag concentration and Gag-membrane association.
Fogarty KH, Berk S, Grigsby IF, Chen Y, Mansky LM, Mueller JD., J Mol Biol 426(7), 2014
PMID: 24316368
Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits.
Van Engelenburg SB, Shtengel G, Sengupta P, Waki K, Jarnik M, Ablan SD, Freed EO, Hess HF, Lippincott-Schwartz J., Science 343(6171), 2014
PMID: 24436186
HIV-1 entry and trans-infection of astrocytes involves CD81 vesicles.
Gray LR, Turville SG, Hitchen TL, Cheng WJ, Ellett AM, Salimi H, Roche MJ, Wesselingh SL, Gorry PR, Churchill MJ., PLoS One 9(2), 2014
PMID: 24587404
ROCK1 and LIM kinase modulate retrovirus particle release and cell-cell transmission events.
Wen X, Ding L, Wang JJ, Qi M, Hammonds J, Chu H, Chen X, Hunter E, Spearman P., J Virol 88(12), 2014
PMID: 24696479
Second generation imaging of nuclear/cytoplasmic HIV-1 complexes.
Francis AC, Di Primio C, Quercioli V, Valentini P, Boll A, Girelli G, Demichelis F, Arosio D, Cereseto A., AIDS Res Hum Retroviruses 30(7), 2014
PMID: 24798748
Live Cell Imaging of Retroviral Entry.
Hulme AE, Hope TJ., Annu Rev Virol 1(1), 2014
PMID: 26958731
Role of the nucleocapsid region in HIV-1 Gag assembly as investigated by quantitative fluorescence-based microscopy.
de Rocquigny H, El Meshri SE, Richert L, Didier P, Darlix JL, Mély Y., Virus Res 193(), 2014
PMID: 25016037
In COS cells Vpu can both stabilize tetherin expression and counteract its antiviral activity.
Waheed AA, Kuruppu ND, Felton KL, D'Souza D, Freed EO., PLoS One 9(10), 2014
PMID: 25360760
Unclosed HIV-1 capsids suggest a curled sheet model of assembly.
Yu Z, Dobro MJ, Woodward CL, Levandovsky A, Danielson CM, Sandrin V, Shi J, Aiken C, Zandi R, Hope TJ, Jensen GJ., J Mol Biol 425(1), 2013
PMID: 23079241
Mechanisms of enhanced HIV spread through T-cell virological synapses.
Dale BM, Alvarez RA, Chen BK., Immunol Rev 251(1), 2013
PMID: 23278744
Efavirenz enhances HIV-1 gag processing at the plasma membrane through Gag-Pol dimerization.
Sudo S, Haraguchi H, Hirai Y, Gatanaga H, Sakuragi J, Momose F, Morikawa Y., J Virol 87(6), 2013
PMID: 23302874
A cis-acting element in retroviral genomic RNA links Gag-Pol ribosomal frameshifting to selective viral RNA encapsidation.
Chamanian M, Purzycka KJ, Wille PT, Ha JS, McDonald D, Gao Y, Le Grice SF, Arts EJ., Cell Host Microbe 13(2), 2013
PMID: 23414758
Revising the Role of Myeloid cells in HIV Pathogenesis.
Aggarwal A, McAllery S, Turville SG., Curr HIV/AIDS Rep 10(1), 2013
PMID: 23242701
Dynamics of HIV-containing compartments in macrophages reveal sequestration of virions and transient surface connections.
Gaudin R, Berre S, Cunha de Alencar B, Decalf J, Schindler M, Gobert FX, Jouve M, Benaroch P., PLoS One 8(7), 2013
PMID: 23922713
Postintegration HIV-1 infection of cervical epithelial cells mediates contact-dependent productive infection of T cells.
Micsenyi AM, Zony C, Alvarez RA, Durham ND, Chen BK, Klotman ME., J Infect Dis 208(11), 2013
PMID: 23908485
Fusion of mature HIV-1 particles leads to complete release of a gag-GFP-based content marker and raises the intraviral pH.
Padilla-Parra S, Marin M, Gahlaut N, Suter R, Kondo N, Melikyan GB., PLoS One 8(8), 2013
PMID: 23951066
Macrophage internal HIV-1 is protected from neutralizing antibodies.
Koppensteiner H, Banning C, Schneider C, Hohenberg H, Schindler M., J Virol 86(5), 2012
PMID: 22205742
Mucosal transmission of human immunodeficiency virus.
Tebit DM, Ndembi N, Weinberg A, Quiñones-Mateu ME., Curr HIV Res 10(1), 2012
PMID: 22264040
In vivo imaging of virological synapses.
Sewald X, Gonzalez DG, Haberman AM, Mothes W., Nat Commun 3(), 2012
PMID: 23271654
Cannabinoid receptor 2-mediated attenuation of CXCR4-tropic HIV infection in primary CD4+ T cells.
Costantino CM, Gupta A, Yewdall AW, Dale BM, Devi LA, Chen BK., PLoS One 7(3), 2012
PMID: 22448282
Neutralization resistance of virological synapse-mediated HIV-1 Infection is regulated by the gp41 cytoplasmic tail.
Durham ND, Yewdall AW, Chen P, Lee R, Zony C, Robinson JE, Chen BK., J Virol 86(14), 2012
PMID: 22553332
Quantitative live-cell imaging of human immunodeficiency virus (HIV-1) assembly.
Baumgärtel V, Müller B, Lamb DC., Viruses 4(5), 2012
PMID: 22754649
Mobilization of HIV spread by diaphanous 2 dependent filopodia in infected dendritic cells.
Aggarwal A, Iemma TL, Shih I, Newsome TP, McAllery S, Cunningham AL, Turville SG., PLoS Pathog 8(6), 2012
PMID: 22685410
Critical role for the kinesin KIF3A in the HIV life cycle in primary human macrophages.
Gaudin R, de Alencar BC, Jouve M, Bèrre S, Le Bouder E, Schindler M, Varthaman A, Gobert FX, Benaroch P., J Cell Biol 199(3), 2012
PMID: 23091068
Tracking and quantitation of fluorescent HIV during cell-to-cell transmission.
Dale BM, McNerney GP, Hübner W, Huser TR, Chen BK., Methods 53(1), 2011
PMID: 20627127
FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ.
Kumar S, Alibhai D, Margineanu A, Laine R, Kennedy G, McGinty J, Warren S, Kelly D, Alexandrov Y, Munro I, Talbot C, Stuckey DW, Kimberly C, Viellerobe B, Lacombe F, Lam EW, Taylor H, Dallman MJ, Stamp G, Murray EJ, Stuhmeier F, Sardini A, Katan M, Elson DS, Elson DS, Neil MA, Dunsby C, French PM., Chemphyschem 12(3), 2011
PMID: 21337485
Virological synapses allow HIV-1 uptake and gene expression in renal tubular epithelial cells.
Chen P, Chen BK, Mosoian A, Hays T, Ross MJ, Klotman PE, Klotman ME., J Am Soc Nephrol 22(3), 2011
PMID: 21335514
Protein transduction by pseudotyped lentivirus-like nanoparticles.
Aoki T, Miyauchi K, Urano E, Ichikawa R, Komano J., Gene Ther 18(9), 2011
PMID: 21451580
Multiploid inheritance of HIV-1 during cell-to-cell infection.
Del Portillo A, Tripodi J, Najfeld V, Wodarz D, Levy DN, Chen BK., J Virol 85(14), 2011
PMID: 21543479
New insights into HTLV-1 particle structure, assembly, and Gag-Gag interactions in living cells.
Fogarty KH, Zhang W, Grigsby IF, Johnson JL, Chen Y, Mueller JD, Mansky LM., Viruses 3(6), 2011
PMID: 21994753
Visualizing HIV-1 assembly.
Jouvenet N, Simon SM, Bieniasz PD., J Mol Biol 410(4), 2011
PMID: 21762796
A SNAP-tagged derivative of HIV-1--a versatile tool to study virus-cell interactions.
Eckhardt M, Anders M, Muranyi W, Heilemann M, Krijnse-Locker J, Müller B., PLoS One 6(7), 2011
PMID: 21799764
New insights into HIV assembly and trafficking.
Balasubramaniam M, Freed EO., Physiology (Bethesda) 26(4), 2011
PMID: 21841072
Cell-to-cell transfer of HIV-1 via virological synapses leads to endosomal virion maturation that activates viral membrane fusion.
Dale BM, McNerney GP, Thompson DL, Hubner W, de Los Reyes K, Chuang FY, Huser T, Chen BK., Cell Host Microbe 10(6), 2011
PMID: 22177560
HIV-1 Vpr oligomerization but not that of Gag directs the interaction between Vpr and Gag.
Fritz JV, Dujardin D, Godet J, Didier P, De Mey J, Darlix JL, Mély Y, de Rocquigny H., J Virol 84(3), 2010
PMID: 19923179
Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1.
McNerney GP, Hübner W, Chen BK, Huser T., J Biophotonics 3(4), 2010
PMID: 20301121
Protein transduction from retroviral Gag precursors.
Voelkel C, Galla M, Maetzig T, Warlich E, Kuehle J, Zychlinski D, Bode J, Cantz T, Schambach A, Baum C., Proc Natl Acad Sci U S A 107(17), 2010
PMID: 20385817
EASY-HIT: HIV full-replication technology for broad discovery of multiple classes of HIV inhibitors.
Kremb S, Helfer M, Heller W, Hoffmann D, Wolff H, Kleinschmidt A, Cepok S, Hemmer B, Durner J, Brack-Werner R., Antimicrob Agents Chemother 54(12), 2010
PMID: 20876377
Visualizing cell-to-cell transfer of HIV using fluorescent clones of HIV and live confocal microscopy.
Dale B, McNerney GP, Thompson DL, Hübner W, Huser T, Chen BK., J Vis Exp (44), 2010
PMID: 20972408
HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components.
Krementsov DN, Rassam P, Margeat E, Roy NH, Schneider-Schaulies J, Milhiet PE, Thali M., Traffic 11(11), 2010
PMID: 20727121
Quantitative 3D video microscopy of HIV transfer across T cell virological synapses.
Hübner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FY, Li XD, Asmuth DM, Huser T, Chen BK., Science 323(5922), 2009
PMID: 19325119
Evidence that productive human immunodeficiency virus type 1 assembly can occur in an intracellular compartment.
Joshi A, Ablan SD, Soheilian F, Nagashima K, Freed EO., J Virol 83(11), 2009
PMID: 19297499
Formation of syncytia is repressed by tetraspanins in human immunodeficiency virus type 1-producing cells.
Weng J, Krementsov DN, Khurana S, Roy NH, Thali M., J Virol 83(15), 2009
PMID: 19458002
Tetraspanins regulate cell-to-cell transmission of HIV-1.
Krementsov DN, Weng J, Lambelé M, Roy NH, Thali M., Retrovirology 6(), 2009
PMID: 19602278
Recent developments of biological reporter technology for detecting gene expression.
Jiang T, Xing B, Rao J., Biotechnol Genet Eng Rev 25(), 2008
PMID: 21412349
Cellular proteins detected in HIV-1.
Ott DE., Rev Med Virol 18(3), 2008
PMID: 18265424
Imaging the biogenesis of individual HIV-1 virions in live cells.
Jouvenet N, Bieniasz PD, Simon SM., Nature 454(7201), 2008
PMID: 18500329
Species barrier of HIV-1 and its jumping by virus engineering.
Nomaguchi M, Doi N, Kamada K, Adachi A., Rev Med Virol 18(4), 2008
PMID: 18386279
The T body, a new cytoplasmic RNA granule in Saccharomyces cerevisiae.
Malagon F, Jensen TH., Mol Cell Biol 28(19), 2008
PMID: 18678648
Mouse mammary tumor virus uses mouse but not human transferrin receptor 1 to reach a low pH compartment and infect cells.
Wang E, Obeng-Adjei N, Ying Q, Meertens L, Dragic T, Davey RA, Ross SR., Virology 381(2), 2008
PMID: 18829060
Live cell imaging of the HIV-1 life cycle.
Campbell EM, Hope TJ., Trends Microbiol 16(12), 2008
PMID: 18977142

57 References

Daten bereitgestellt von Europe PubMed Central.

Inhibition of viral assembly in murine cells by HIV-1 matrix.
Hubner W, Chen BK., Virology 352(1), 2006
PMID: 16750235
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A., Nat. Biotechnol. 20(1), 2002
PMID: 11753368
Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.
Zacharias DA, Violin JD, Newton AC, Tsien RY., Science 296(5569), 2002
PMID: 11988576
Human macrophages accumulate HIV-1 particles in MHC II compartments.
Raposo G, Moore M, Innes D, Leijendekker R, Leigh-Brown A, Benaroch P, Geuze H., Traffic 3(10), 2002
PMID: 12230470
FRET or no FRET: a quantitative comparison.
Berney C, Danuser G., Biophys. J. 84(6), 2003
PMID: 12770904
Spectral imaging and its applications in live cell microscopy.
Zimmermann T, Rietdorf J, Pepperkok R., FEBS Lett. 546(1), 2003
PMID: 12829241
Retroviral genomic RNAs are transported to the plasma membrane by endosomal vesicles.
Basyuk E, Galli T, Mougel M, Blanchard JM, Sitbon M, Bertrand E., Dev. Cell 5(1), 2003
PMID: 12852860
Infectious HIV-1 assembles in late endosomes in primary macrophages.
Pelchen-Matthews A, Kramer B, Marsh M., J. Cell Biol. 162(3), 2003
PMID: 12885763
HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein.
Pornillos O, Higginson DS, Stray KM, Fisher RD, Garrus JE, Payne M, He GP, Wang HE, Morham SG, Sundquist WI., J. Cell Biol. 162(3), 2003
PMID: 12900394
Visualization of retroviral replication in living cells reveals budding into multivesicular bodies.
Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W., Traffic 4(11), 2003
PMID: 14617360
HIV-1 egress is gated through late endosomal membranes.
Nydegger S, Foti M, Derdowski A, Spearman P, Thali M., Traffic 4(12), 2003
PMID: 14617353
Viral protein U counteracts a human host cell restriction that inhibits HIV-1 particle production.
Varthakavi V, Smith RM, Bour SP, Strebel K, Spearman P., Proc. Natl. Acad. Sci. U.S.A. 100(25), 2003
PMID: 14657387
Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-II by the AP-1 gamma-sigma1 and AP-3 delta-sigma3 hemicomplexes.
Janvier K, Kato Y, Boehm M, Rose JR, Martina JA, Kim BY, Venkatesan S, Bonifacino JS., J. Cell Biol. 163(6), 2003
PMID: 14691137
HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse.
Jolly C, Kashefi K, Hollinshead M, Sattentau QJ., J. Exp. Med. 199(2), 2004
PMID: 14734528
An improved cyan fluorescent protein variant useful for FRET.
Rizzo MA, Springer GH, Granada B, Piston DW., Nat. Biotechnol. 22(4), 2004
PMID: 14990965
Retroviral mRNA nuclear export elements regulate protein function and virion assembly.
Swanson CM, Puffer BA, Ahmad KM, Doms RW, Malim MH., EMBO J. 23(13), 2004
PMID: 15201866
Human immunodeficiency virus type 1 matrix inhibits and confers cooperativity on gag precursor-membrane interactions.
Perez-Caballero D, Hatziioannou T, Martin-Serrano J, Bieniasz PD., J. Virol. 78(17), 2004
PMID: 15308748
The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding.
Stuchell MD, Garrus JE, Muller B, Stray KM, Ghaffarian S, McKinnon R, Krausslich HG, Morham SG, Sundquist WI., J. Biol. Chem. 279(34), 2004
PMID: 15218037
Dangerous liaisons at the virological synapse.
Piguet V, Sattentau Q., J. Clin. Invest. 114(5), 2004
PMID: 15343375
Construction and characterization of a fluorescently labeled infectious human immunodeficiency virus type 1 derivative.
Muller B, Daecke J, Fackler OT, Dittmar MT, Zentgraf H, Krausslich HG., J. Virol. 78(19), 2004
PMID: 15367647
Retrovirus budding.
Morita E, Sundquist WI., Annu. Rev. Cell Dev. Biol. 20(), 2004
PMID: 15473846
Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.
Adachi A, Gendelman HE, Koenig S, Folks T, Willey R, Rabson A, Martin MA., J. Virol. 59(2), 1986
PMID: 3016298
Dissociation of gp120 from HIV-1 virions induced by soluble CD4.
Moore JP, McKeating JA, Weiss RA, Sattentau QJ., Science 250(4984), 1990
PMID: 2251501
Human immunodeficiency virus envelope protein determines the site of virus release in polarized epithelial cells.
Owens RJ, Dubay JW, Hunter E, Compans RW., Proc. Natl. Acad. Sci. U.S.A. 88(9), 1991
PMID: 2023946
The intracytoplasmic domain of gp41 mediates polarized budding of human immunodeficiency virus type 1 in MDCK cells.
Lodge R, Gottlinger H, Gabuzda D, Cohen EA, Lemay G., J. Virol. 68(8), 1994
PMID: 8035484
Specific interactions between retrovirus Env and Gag proteins in rat neurons.
Weclewicz K, Ekstrom M, Kristensson K, Garoff H., J. Virol. 72(4), 1998
PMID: 9525603
Leucine-specific, functional interactions between human immunodeficiency virus type 1 Nef and adaptor protein complexes.
Coleman SH, Van Damme N, Day JR, Noviello CM, Hitchin D, Madrid R, Benichou S, Guatelli JC., J. Virol. 79(4), 2005
PMID: 15681409
AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assembly.
Dong X, Li H, Derdowski A, Ding L, Burnett A, Chen X, Peters TR, Dermody TS, Woodruff E, Wang JJ, Spearman P., Cell 120(5), 2005
PMID: 15766529
Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling.
Rudner L, Nydegger S, Coren LV, Nagashima K, Thali M, Ott DE., J. Virol. 79(7), 2005
PMID: 15767407
Involvement of HIV-1 protease in virus-induced cell killing.
Ventoso I, Navarro J, Munoz MA, Carrasco L., Antiviral Res. 66(1), 2005
PMID: 15781132
HIV Nef-mediated CD4 down-regulation is adaptor protein complex 2 dependent.
Jin YJ, Cai CY, Zhang X, Zhang HT, Hirst JA, Burakoff SJ., J. Immunol. 175(5), 2005
PMID: 16116206
Plasma membrane is the site of productive HIV-1 particle assembly.
Jouvenet N, Neil SJ, Bess C, Johnson MC, Virgen CA, Simon SM, Bieniasz PD., PLoS Biol. 4(12), 2006
PMID: 17147474
In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53.
Deneka M, Pelchen-Matthews A, Byland R, Ruiz-Mateos E, Marsh M., J. Cell Biol. 177(2), 2007
PMID: 17438075
HIV-1 buds predominantly at the plasma membrane of primary human macrophages.
Welsch S, Keppler OT, Habermann A, Allespach I, Krijnse-Locker J, Krausslich HG., PLoS Pathog. 3(3), 2007
PMID: 17381240
Visualization of retrovirus budding with correlated light and electron microscopy.
Larson DR, Johnson MC, Webb WW, Vogt VM., Proc. Natl. Acad. Sci. U.S.A. 102(43), 2005
PMID: 16230638
The pericentriolar recycling endosome plays a key role in Vpu-mediated enhancement of HIV-1 particle release.
Varthakavi V, Smith RM, Martin KL, Derdowski A, Lapierre LA, Goldenring JR, Spearman P., Traffic 7(3), 2006
PMID: 16497224
Vpu and Tsg101 regulate intracellular targeting of the human immunodeficiency virus type 1 core protein precursor Pr55gag.
Harila K, Prior I, Sjoberg M, Salminen A, Hinkula J, Suomalainen M., J. Virol. 80(8), 2006
PMID: 16571793
Fanciful FRET.
Vogel SS, Thaler C, Koushik SV., Sci. STKE 2006(331), 2006
PMID: 16622184
HIV-1 Vpu promotes release and prevents endocytosis of nascent retrovirus particles from the plasma membrane.
Neil SJ, Eastman SW, Jouvenet N, Bieniasz PD., PLoS Pathog. 2(5), 2006
PMID: 16699598

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17728233
PubMed | Europe PMC

Suchen in

Google Scholar