Dystroglycan is associated to the disulfide isomerase ERp57

Sciandra F, Angelucci E, Altieri F, Ricci D, Hübner W, Petrucci TC, Giardina B, Brancaccio A, Bozzi M (2012)
Experimental cell research 318(19): 2460-2469.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ; ; ; ;
Abstract / Bemerkung
Dystroglycan (DG) is an extracellular receptor composed of two subunits, α-DG and β-DG, connected through the α-DG C-terminal domain and the β-DG N-terminal domain. We report an alanine scanning of all DG cysteine residues performed on DG-GFP constructs overexpressed in 293-Ebna cells, demonstrating that Cys-669 and Cys-713, both located within the β-DG N-terminal domain, are key residues for the DG precursor cleavage and trafficking, but not for the interaction between the two DG subunits. In addition, we have used immunprecipitation and confocal microscopy showing that ERp57, a member of the disulfide isomerase family involved in glycoprotein folding, is associated and colocalizes immunohistochemically with β-DG in the ER and at the plasma membrane of 293-Ebna cells. The β-DG-ERp57 complex also included α-DG. DG mutants, unable to undergo the precursor cleavage, were still associated to ERp57. β-DG and ERp57 were also co-immunoprecipitated in rat heart and kidney tissues. In vitro, a mutant ERp57, mimicking the reduced form of the wild-type protein, interacts directly with the recombinant N-terminal domain of both α-DG and β-DG with apparent dissociation constant values in the micromolar range. ERp57 is likely to be involved in the DG processing/maturation pathway, but its association to the mature DG complex might also suggest some further functional role that needs to be investigated.
Stichworte
Protein Binding/genetics; Mutation; Kidney/physiology; Kidney/metabolism; Humans; Heart/physiology; HEK293 Cells; Glycosylation; Dystroglycans/metabolism; Dystroglycans/genetics; Cultured; Cells; Cell Membrane/metabolism; Animals; Cell Membrane/genetics; Protein Binding/physiology; Protein Disulfide-Isomerases/metabolism; Protein Structure; Tertiary; Protein Subunits; Protein Transport/physiology; Rats
Erscheinungsjahr
2012
Zeitschriftentitel
Experimental cell research
Band
318
Ausgabe
19
Seite(n)
2460-2469
ISSN
0014-4827
Page URI
https://pub.uni-bielefeld.de/record/2576808

Zitieren

Sciandra F, Angelucci E, Altieri F, et al. Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research. 2012;318(19):2460-2469.
Sciandra, F., Angelucci, E., Altieri, F., Ricci, D., Hübner, W., Petrucci, T. C., Giardina, B., et al. (2012). Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research, 318(19), 2460-2469. doi:10.1016/j.yexcr.2012.07.006
Sciandra, F., Angelucci, E., Altieri, F., Ricci, D., Hübner, W., Petrucci, T. C., Giardina, B., Brancaccio, A., and Bozzi, M. (2012). Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research 318, 2460-2469.
Sciandra, F., et al., 2012. Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research, 318(19), p 2460-2469.
F. Sciandra, et al., “Dystroglycan is associated to the disulfide isomerase ERp57”, Experimental cell research, vol. 318, 2012, pp. 2460-2469.
Sciandra, F., Angelucci, E., Altieri, F., Ricci, D., Hübner, W., Petrucci, T.C., Giardina, B., Brancaccio, A., Bozzi, M.: Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research. 318, 2460-2469 (2012).
Sciandra, Francesca, Angelucci, Emanuela, Altieri, Fabio, Ricci, Daniela, Hübner, Wolfgang, Petrucci, Tamara C, Giardina, Bruno, Brancaccio, Andrea, and Bozzi, Manuela. “Dystroglycan is associated to the disulfide isomerase ERp57”. Experimental cell research 318.19 (2012): 2460-2469.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 22814252
PubMed | Europe PMC

Suchen in

Google Scholar