Dystroglycan is associated to the disulfide isomerase ERp57

Sciandra F, Angelucci E, Altieri F, Ricci D, Hübner W, Petrucci TC, Giardina B, Brancaccio A, Bozzi M (2012)
Experimental cell research 318(19): 2460-2469.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ; ; ; ;
Abstract / Bemerkung
Dystroglycan (DG) is an extracellular receptor composed of two subunits, α-DG and β-DG, connected through the α-DG C-terminal domain and the β-DG N-terminal domain. We report an alanine scanning of all DG cysteine residues performed on DG-GFP constructs overexpressed in 293-Ebna cells, demonstrating that Cys-669 and Cys-713, both located within the β-DG N-terminal domain, are key residues for the DG precursor cleavage and trafficking, but not for the interaction between the two DG subunits. In addition, we have used immunprecipitation and confocal microscopy showing that ERp57, a member of the disulfide isomerase family involved in glycoprotein folding, is associated and colocalizes immunohistochemically with β-DG in the ER and at the plasma membrane of 293-Ebna cells. The β-DG-ERp57 complex also included α-DG. DG mutants, unable to undergo the precursor cleavage, were still associated to ERp57. β-DG and ERp57 were also co-immunoprecipitated in rat heart and kidney tissues. In vitro, a mutant ERp57, mimicking the reduced form of the wild-type protein, interacts directly with the recombinant N-terminal domain of both α-DG and β-DG with apparent dissociation constant values in the micromolar range. ERp57 is likely to be involved in the DG processing/maturation pathway, but its association to the mature DG complex might also suggest some further functional role that needs to be investigated.
Erscheinungsjahr
Zeitschriftentitel
Experimental cell research
Band
318
Ausgabe
19
Seite(n)
2460-2469
ISSN
PUB-ID

Zitieren

Sciandra F, Angelucci E, Altieri F, et al. Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research. 2012;318(19):2460-2469.
Sciandra, F., Angelucci, E., Altieri, F., Ricci, D., Hübner, W., Petrucci, T. C., Giardina, B., et al. (2012). Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research, 318(19), 2460-2469. doi:10.1016/j.yexcr.2012.07.006
Sciandra, F., Angelucci, E., Altieri, F., Ricci, D., Hübner, W., Petrucci, T. C., Giardina, B., Brancaccio, A., and Bozzi, M. (2012). Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research 318, 2460-2469.
Sciandra, F., et al., 2012. Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research, 318(19), p 2460-2469.
F. Sciandra, et al., “Dystroglycan is associated to the disulfide isomerase ERp57”, Experimental cell research, vol. 318, 2012, pp. 2460-2469.
Sciandra, F., Angelucci, E., Altieri, F., Ricci, D., Hübner, W., Petrucci, T.C., Giardina, B., Brancaccio, A., Bozzi, M.: Dystroglycan is associated to the disulfide isomerase ERp57. Experimental cell research. 318, 2460-2469 (2012).
Sciandra, Francesca, Angelucci, Emanuela, Altieri, Fabio, Ricci, Daniela, Hübner, Wolfgang, Petrucci, Tamara C, Giardina, Bruno, Brancaccio, Andrea, and Bozzi, Manuela. “Dystroglycan is associated to the disulfide isomerase ERp57”. Experimental cell research 318.19 (2012): 2460-2469.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

A dystroglycan mutation (p.Cys667Phe) associated to muscle-eye-brain disease with multicystic leucodystrophy results in ER-retention of the mutant protein.
Signorino G, Covaceuszach S, Bozzi M, Hübner W, Mönkemöller V, Konarev PV, Cassetta A, Brancaccio A, Sciandra F., Hum Mutat 39(2), 2018
PMID: 29134705
Quantification, 2DE analysis and identification of enriched glycosylated proteins from mouse muscles: Difficulties and alternatives.
de Fátima MenegociEugênio P, Assunção NA, Sciandra F, Aquino A, Brancaccio A, Carrilho E., Electrophoresis 37(2), 2016
PMID: 26542084
Increased levels of expression of dystroglycan may protect the heart.
Brancaccio A., Neuromuscul Disord 23(11), 2013
PMID: 23911074
Insertion of a myc-tag within α-dystroglycan domains improves its biochemical and microscopic detection.
Morlacchi S, Sciandra F, Bigotti MG, Bozzi M, Hübner W, Galtieri A, Giardina B, Brancaccio A., BMC Biochem 13(), 2012
PMID: 22835149

36 References

Daten bereitgestellt von Europe PubMed Central.

ERp57 is essential for efficient folding of glycoproteins sharing common structural domains.
Jessop CE, Chakravarthi S, Garbi N, Hammerling GJ, Lovell S, Bulleid NJ., EMBO J. 26(1), 2006
PMID: 17170699
Concerted mutation of Phe residues belonging to the beta-dystroglycan ectodomain strongly inhibits the interaction with alpha-dystroglycan in vitro.
Bozzi M, Sciandra F, Ferri L, Torreri P, Pavoni E, Petrucci TC, Giardina B, Brancaccio A., FEBS J. 273(21), 2006
PMID: 17018058
SEA domain proteolysis determines the functional composition of dystroglycan.
Akhavan A, Crivelli SN, Singh M, Lingappa VR, Muschler JL., FASEB J. 22(2), 2007
PMID: 17905726
Muscular dystrophies due to glycosylation defects.
Muntoni F, Torelli S, Brockington M., Neurotherapeutics 5(4), 2008
PMID: 19019316
Substrate specificity of the oxidoreductase ERp57 is determined primarily by its interaction with calnexin and calreticulin.
Jessop CE, Tavender TJ, Watkins RH, Chambers JE, Bulleid NJ., J. Biol. Chem. 284(4), 2008
PMID: 19054761
Functional diversity of dystroglycan.
Bozzi M, Morlacchi S, Bigotti MG, Sciandra F, Brancaccio A., Matrix Biol. 28(4), 2009
PMID: 19303439
Mutagenesis at the alpha-beta interface impairs the cleavage of the dystroglycan precursor.
Sciandra F, Bozzi M, Morlacchi S, Galtieri A, Giardina B, Brancaccio A., FEBS J. 276(17), 2009
PMID: 19694806
Role of ERp57 in the signaling and transcriptional activity of STAT3 in a melanoma cell line.
Chichiarelli S, Gaucci E, Ferraro A, Grillo C, Altieri F, Cocchiola R, Arcangeli V, Turano C, Eufemi M., Arch. Biochem. Biophys. 494(2), 2009
PMID: 19995546
A second Ig-like domain identified in dystroglycan by molecular modelling and dynamics.
De Rosa MC, Pirolli D, Bozzi M, Sciandra F, Giardina B, Brancaccio A., J. Mol. Graph. Model. 29(8), 2011
PMID: 21605994
ERp57/GRP58: a protein with multiple functions.
Turano C, Gaucci E, Grillo C, Chichiarelli S., Cell. Mol. Biol. Lett. 16(4), 2011
PMID: 21837552
The platelet-surface thiol isomerase enzyme ERp57 modulates platelet function.
Holbrook LM, Sasikumar P, Stanley RG, Simmonds AD, Bicknell AB, Gibbins JM., J. Thromb. Haemost. 10(2), 2012
PMID: 22168334
Insertion of a myc-tag within α-dystroglycan domains improves its biochemical and microscopic detection.
Morlacchi S, Sciandra F, Bigotti MG, Bozzi M, Hubner W, Galtieri A, Giardina B, Brancaccio A., BMC Biochem. 13(), 2012
PMID: 22835149
Structural and functional analysis of the N-terminal extracellular region of beta-dystroglycan.
Di Stasio E, Sciandra F, Maras B, Di Tommaso F, Petrucci TC, Giardina B, Brancaccio A., Biochem. Biophys. Res. Commun. 266(1), 1999
PMID: 10581202
Biosynthesis of dystroglycan: processing of a precursor propeptide.
Holt KH, Crosbie RH, Venzke DP, Campbell KP., FEBS Lett. 468(1), 2000
PMID: 10683445
Physical proximity and functional association of glycoprotein 1balpha and protein-disulfide isomerase on the platelet plasma membrane.
Burgess JK, Hotchkiss KA, Suter C, Dudman NP, Szollosi J, Chesterman CN, Chong BH, Hogg PJ., J. Biol. Chem. 275(13), 2000
PMID: 10734129
The complexities of dystroglycan.
Winder SJ., Trends Biochem. Sci. 26(2), 2001
PMID: 11166570
Nuclear localization and DNA interaction of protein disulfide isomerase ERp57 in mammalian cells.
Coppari S, Altieri F, Ferraro A, Chichiarelli S, Eufemi M, Turano C., J. Cell. Biochem. 85(2), 2002
PMID: 11948688
Formation and transfer of disulphide bonds in living cells.
Sevier CS, Kaiser CA., Nat. Rev. Mol. Cell Biol. 3(11), 2002
PMID: 12415301
Disulfide bonds as switches for protein function.
Hogg PJ., Trends Biochem. Sci. 28(4), 2003
PMID: 12713905
Inhibition of dystroglycan cleavage causes muscular dystrophy in transgenic mice.
Jayasinha V, Nguyen HH, Xia B, Kammesheidt A, Hoyte K, Martin PT., Neuromuscul. Disord. 13(5), 2003
PMID: 12798792
The effects of post-translational processing on dystroglycan synthesis and trafficking.
Esapa CT, Bentham GR, Schroder JE, Kroger S, Blake DJ., FEBS Lett. 555(2), 2003
PMID: 14644417
Ribozyme knockdown functionally links a 1,25(OH)2D3 membrane binding protein (1,25D3-MARRS) and phosphate uptake in intestinal cells.
Nemere I, Farach-Carson MC, Rohe B, Sterling TM, Norman AW, Boyan BD, Safford SE., Proc. Natl. Acad. Sci. U.S.A. 101(19), 2004
PMID: 15123837
ERp57 is present in STAT3-DNA complexes.
Eufemi M, Coppari S, Altieri F, Grillo C, Ferraro A, Turano C., Biochem. Biophys. Res. Commun. 323(4), 2004
PMID: 15451439
Amino acid determinants that drive heparan sulfate assembly in a proteoglycan.
Zhang L, Esko JD., J. Biol. Chem. 269(30), 1994
PMID: 8034692
Interaction of the thiol-dependent reductase ERp57 with nascent glycoproteins.
Oliver JD, van der Wal FJ, Bulleid NJ, High S., Science 275(5296), 1997
PMID: 8974399
Scanning and escape during protein-disulfide isomerase-assisted protein folding.
Walker KW, Gilbert HF., J. Biol. Chem. 272(14), 1997
PMID: 9082998
The N-terminal region of alpha-dystroglycan is an autonomous globular domain.
Brancaccio A, Schulthess T, Gesemann M, Engel J., Eur. J. Biochem. 246(1), 1997
PMID: 9210479
A single disulfide bridge (Cys182-Cys264) is crucial for alpha-dystroglycan N-terminal domain stability.
Brancaccio A, Jeno P, Engel J., Ann. N. Y. Acad. Sci. 857(), 1998
PMID: 9917844
Cellular physiology of STAT3: Where's the cytoplasmic monomer?
Ndubuisi MI, Guo GG, Fried VA, Etlinger JD, Sehgal PB., J. Biol. Chem. 274(36), 1999
PMID: 10464281
A role for the thiol isomerase protein ERP5 in platelet function.
Jordan PA, Stevens JM, Hubbard GP, Barrett NE, Sage T, Authi KS, Gibbins JM., Blood 105(4), 2004
PMID: 15466936
Dystroglycan: from biosynthesis to pathogenesis of human disease.
Barresi R, Campbell KP., J. Cell. Sci. 119(Pt 2), 2006
PMID: 16410545
Extracellular disulfide exchange and the regulation of cellular function.
Jordan PA, Gibbins JM., Antioxid. Redox Signal. 8(3-4), 2006
PMID: 16677077
Cooperative activity of Ref-1/APE and ERp57 in reductive activation of transcription factors.
Grillo C, D'Ambrosio C, Scaloni A, Maceroni M, Merluzzi S, Turano C, Altieri F., Free Radic. Biol. Med. 41(7), 2006
PMID: 16962936
Cys669-Cys713 disulfide bridge formation is a key to dystroglycan cleavage and subunit association.
Watanabe N, Sasaoka T, Noguchi S, Nishino I, Tanaka T., Genes Cells 12(1), 2007
PMID: 17212656

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 22814252
PubMed | Europe PMC

Suchen in

Google Scholar