Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1

McNerney GP, Hübner W, Chen BK, Huser T (2010)
Journal of biophotonics 3(4): 216-223.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Cell-cell interactions through direct contact are very important for cellular communication and coordination - especially for immune cells. The human immunodeficiency virus type I (HIV-1) induces immune cell interactions between CD4(+) cells to shuttle between T cells via a virological synapse. A goal to understand the process of cell-cell transmission through virological synapses is to determine the cellular states that allow a chance encounter between cells to become a stable cell-cell adhesion. We demonstrate the use of optical tweezers to manipulate uninfected primary CD4(+) T cells near HIV Gag-iGFP transfected Jurkat T cells to probe the determinants that induce stable adhesion. When combined with fast 4D confocal fluorescence microscopy, optical tweezers can be utilized not only to facilitate cell-cell contact, but also to simultaneously track the formation of a virological synapse, and ultimately to probe the events that precede virus transfer.
Stichworte
Microscopy; Confocal/methods; Microscopy; Confocal/instrumentation; Microscopy; Jurkat Cells; Intercellular Junctions/virology; Intercellular Junctions/immunology; Humans; HIV-1/physiology; Green Fluorescent Proteins/metabolism; Green Fluorescent Proteins/genetics; Cultured; Cells; Cell Polarity/immunology; Cell Adhesion/immunology; CD4-Positive T-Lymphocytes/virology; CD4-Positive T-Lymphocytes/cytology; CD4-Positive T-Lymphocytes/radiation effects; Fluorescence/instrumentation; Microscopy; Fluorescence/methods; Optical Tweezers; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Transduction; Genetic; gag Gene Products; Human Immunodeficiency Virus/genetics; gag Gene Products; Human Immunodeficiency Virus/metabolism
Erscheinungsjahr
2010
Zeitschriftentitel
Journal of biophotonics
Band
3
Ausgabe
4
Seite(n)
216-223
ISSN
1864-063X
eISSN
1864-0648
Page URI
https://pub.uni-bielefeld.de/record/2576759

Zitieren

McNerney GP, Hübner W, Chen BK, Huser T. Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of biophotonics. 2010;3(4):216-223.
McNerney, G. P., Hübner, W., Chen, B. K., & Huser, T. (2010). Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of biophotonics, 3(4), 216-223. doi:10.1002/jbio.200900102
McNerney, G. P., Hübner, W., Chen, B. K., and Huser, T. (2010). Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of biophotonics 3, 216-223.
McNerney, G.P., et al., 2010. Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of biophotonics, 3(4), p 216-223.
G.P. McNerney, et al., “Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”, Journal of biophotonics, vol. 3, 2010, pp. 216-223.
McNerney, G.P., Hübner, W., Chen, B.K., Huser, T.: Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of biophotonics. 3, 216-223 (2010).
McNerney, Gregory P, Hübner, Wolfgang, Chen, Benjamin K, and Huser, Thomas. “Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”. Journal of biophotonics 3.4 (2010): 216-223.

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