Tracking and quantitation of fluorescent HIV during cell-to-cell transmission

Dale BM, McNerney GP, Hübner W, Huser TR, Chen BK (2011)
Methods (San Diego, Calif.) 53(1): 20-26.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The green fluorescent protein (GFP) is a powerful genetic marking tool that has enabled virologists to monitor and track viral proteins during HIV infection. Expression-optimized Gag-GFP constructs have been used to study virus-like particle (VLP) assembly and localization in cell types that are easily transfected. The development of HIV-1 variants carrying GFP within the context of the viral genome has facilitated the study of infection and has been particularly useful in monitoring the transfer of virus between cells following virological synapse formation. HIV Gag-iGFP, a viral clone that contains GFP inserted between the matrix (MA) and capsid (CA) domains of Gag, is the first replication competent molecular clone that generates fluorescent infectious particles. Here, we discuss some methods that exploit HIV Gag-iGFP to quantify cell-to-cell transmission of virus by flow cytometry and to track the proteins during assembly and transmission using live-cell imaging.
Stichworte
Jurkat Cells; Humans; Host-Pathogen Interactions; HIV-1/genetics; HIV Infections/virology; Green Fluorescent Proteins/genetics; Viral; Genome; Flow Cytometry; Viral; Cell Tracking/methods; Cytopathogenic Effect; Microscopy; Fluorescence; Organisms; Genetically Modified; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/genetics; gag Gene Products; Human Immunodeficiency Virus/biosynthesis; gag Gene Products; Human Immunodeficiency Virus/genetics
Erscheinungsjahr
2011
Zeitschriftentitel
Methods (San Diego, Calif.)
Band
53
Ausgabe
1
Seite(n)
20-26
ISSN
1046-2023
Page URI
https://pub.uni-bielefeld.de/record/2576752

Zitieren

Dale BM, McNerney GP, Hübner W, Huser TR, Chen BK. Tracking and quantitation of fluorescent HIV during cell-to-cell transmission. Methods (San Diego, Calif.). 2011;53(1):20-26.
Dale, B. M., McNerney, G. P., Hübner, W., Huser, T. R., & Chen, B. K. (2011). Tracking and quantitation of fluorescent HIV during cell-to-cell transmission. Methods (San Diego, Calif.), 53(1), 20-26. doi:10.1016/j.ymeth.2010.06.018
Dale, B. M., McNerney, G. P., Hübner, W., Huser, T. R., and Chen, B. K. (2011). Tracking and quantitation of fluorescent HIV during cell-to-cell transmission. Methods (San Diego, Calif.) 53, 20-26.
Dale, B.M., et al., 2011. Tracking and quantitation of fluorescent HIV during cell-to-cell transmission. Methods (San Diego, Calif.), 53(1), p 20-26.
B.M. Dale, et al., “Tracking and quantitation of fluorescent HIV during cell-to-cell transmission”, Methods (San Diego, Calif.), vol. 53, 2011, pp. 20-26.
Dale, B.M., McNerney, G.P., Hübner, W., Huser, T.R., Chen, B.K.: Tracking and quantitation of fluorescent HIV during cell-to-cell transmission. Methods (San Diego, Calif.). 53, 20-26 (2011).
Dale, Benjamin M, McNerney, Gregory P, Hübner, Wolfgang, Huser, Thomas R, and Chen, Benjamin K. “Tracking and quantitation of fluorescent HIV during cell-to-cell transmission”. Methods (San Diego, Calif.) 53.1 (2011): 20-26.

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PMID: 20627127
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