New highly polymorphic microsatellite loci for the Galapagos marine iguana, Amblyrhynchus cristatus

MacLeod A, Koch V, Garcia-Parra C, Trillmich F, Steinfartz S (2012)
Amphibia-Reptilia 33(3-4): 533-536.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor/in
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Abstract / Bemerkung
We describe the development and characterisation of six new dinucleotide motif microsatellite loci for populations of marine iguanas (Amblyrhynchus cristatus), endemic to the Galapagos archipelago. Primers were based on microsatellite-bearing sequences and initially developed using universally labelled primers. When analysed across 5 populations (representing 150 individuals), new loci displayed, on average, high levels of genetic diversity (range: 2-13 alleles, mean: 5.73) and values of heterozygosity (range: 0.0-0.906, mean: 0.605). No consistent deviations from Hardy-Weinberg equilibrium or significant linkage disequilibrium were observed, and all loci were shown to be free of common microsatellite errors. Utilising the 13 previously available microsatellite loci for this species, we describe here four multiplex combinations for the successful amplification of 19 microsatellite loci for marine iguanas. This powerful set of highly polymorphic markers will allow researchers to explore future questions regarding the ecology, evolution, and conservation of this unique species.
Stichworte
island; Population genetics; genetic effective population size; primer development; populations
Erscheinungsjahr
2012
Zeitschriftentitel
Amphibia-Reptilia
Band
33
Ausgabe
3-4
Seite(n)
533-536
ISSN
0173-5373
eISSN
1568-5381
Page URI
https://pub.uni-bielefeld.de/record/2553397

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MacLeod A, Koch V, Garcia-Parra C, Trillmich F, Steinfartz S. New highly polymorphic microsatellite loci for the Galapagos marine iguana, Amblyrhynchus cristatus. Amphibia-Reptilia. 2012;33(3-4):533-536.
MacLeod, A., Koch, V., Garcia-Parra, C., Trillmich, F., & Steinfartz, S. (2012). New highly polymorphic microsatellite loci for the Galapagos marine iguana, Amblyrhynchus cristatus. Amphibia-Reptilia, 33(3-4), 533-536. doi:10.1163/15685381-00002854
MacLeod, A., Koch, V., Garcia-Parra, C., Trillmich, F., and Steinfartz, S. (2012). New highly polymorphic microsatellite loci for the Galapagos marine iguana, Amblyrhynchus cristatus. Amphibia-Reptilia 33, 533-536.
MacLeod, A., et al., 2012. New highly polymorphic microsatellite loci for the Galapagos marine iguana, Amblyrhynchus cristatus. Amphibia-Reptilia, 33(3-4), p 533-536.
A. MacLeod, et al., “New highly polymorphic microsatellite loci for the Galapagos marine iguana, Amblyrhynchus cristatus”, Amphibia-Reptilia, vol. 33, 2012, pp. 533-536.
MacLeod, A., Koch, V., Garcia-Parra, C., Trillmich, F., Steinfartz, S.: New highly polymorphic microsatellite loci for the Galapagos marine iguana, Amblyrhynchus cristatus. Amphibia-Reptilia. 33, 533-536 (2012).
MacLeod, Amy, Koch, Volker, Garcia-Parra, Carolina, Trillmich, Fritz, and Steinfartz, Sebastian. “New highly polymorphic microsatellite loci for the Galapagos marine iguana, Amblyrhynchus cristatus”. Amphibia-Reptilia 33.3-4 (2012): 533-536.