ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76

Tegetmeyer H, Jones SCP, Langford PR, Baltes N (2008)
Veterinary Microbiology 128(3-4): 342-353.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Tegetmeyer, HalinaUniBi ; Jones, Sophie CP; Langford, Paul R; Baltes, Nina
Abstract / Bemerkung
Actinobacillus pleuropneumoniae, a gram-negative rod of the Pasteurellaceae family, causes pleuropneumonia in pigs. Establishing A. pleuropneumoniae free herds is difficult due to the occurrence of persistently infected animals. The ApxIV toxin is expressed by A. pleuropneumoniae in vivo and an ELISA based on the toxin is used to detect infection and to differentiate between infected and vaccinated animals. In this study, we have identified a 1070bp insertion element of the IS30 family, designated ISApl1, in the A. pleuropneumoniae serotype 7 strain AP76. ISApl1 contains a 924bp ORF encoding a transposase, which is flanked by 27bp inverted repeats showing six mismatches. We investigated the occurrence of ISApl1 in other A. pleuropneumoniae strains, and its possible interference with virulence associated factors. Four insertion sites were identified in AP76: within the apxIVA toxin ORF, within a putative autotransporter adhesin ORF, upstream of a capsular polysaccharide biosynthesis gene cluster, and downstream of a beta-lactamase gene. ISApl1 is also present in some serotype 7 field isolates, but not in reference or field strains of other serotypes. In A. pleuropneumoniae AP76, the transposase gene is transcribed in vitro. The insertion in the apxIVA toxin gene remains stable after animal passage. Since this insertion should disrupt toxin expression, we tested 7 pigs infected with AP76 at day 21 post-infection. All were negative in the ApxIV ELISA but four out of seven were positive in an ApxII toxin ELISA. These results show that insertion elements can affect the detection of A. pleuropneumoniae infected animals.
Veterinary Microbiology
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Tegetmeyer H, Jones SCP, Langford PR, Baltes N. ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76. Veterinary Microbiology. 2008;128(3-4):342-353.
Tegetmeyer, H., Jones, S. C. P., Langford, P. R., & Baltes, N. (2008). ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76. Veterinary Microbiology, 128(3-4), 342-353. doi:10.1016/j.vetmic.2007.10.025
Tegetmeyer, H., Jones, S. C. P., Langford, P. R., and Baltes, N. (2008). ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76. Veterinary Microbiology 128, 342-353.
Tegetmeyer, H., et al., 2008. ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76. Veterinary Microbiology, 128(3-4), p 342-353.
H. Tegetmeyer, et al., “ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76”, Veterinary Microbiology, vol. 128, 2008, pp. 342-353.
Tegetmeyer, H., Jones, S.C.P., Langford, P.R., Baltes, N.: ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76. Veterinary Microbiology. 128, 342-353 (2008).
Tegetmeyer, Halina, Jones, Sophie CP, Langford, Paul R, and Baltes, Nina. “ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76”. Veterinary Microbiology 128.3-4 (2008): 342-353.
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36 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

The ISApl1 2 Dimer Circular Intermediate Participates in mcr-1 Transposition.
He YZ, Li XP, Miao YY, Lin J, Sun RY, Wang XP, Guo YY, Liao XP, Liu YH, Feng Y, Sun J., Front Microbiol 10(), 2019
PMID: 30723461
Update on Actinobacillus pleuropneumoniae-knowledge, gaps and challenges.
Sassu EL, Bossé JT, Tobias TJ, Gottschalk M, Langford PR, Hennig-Pauka I., Transbound Emerg Dis 65 Suppl 1(), 2018
PMID: 29083117
Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant.
Ito H, Takahashi S, Asai T, Tamura Y, Yamamoto K., J Vet Diagn Invest 30(1), 2018
PMID: 29145759
Detection of mcr-1 Gene among Escherichia coli Isolates from Farmed Fish and Characterization of mcr-1-Bearing IncP Plasmids.
Lv L, Cao Y, Yu P, Huang R, Wang J, Wen Q, Zhi C, Zhang Q, Liu JH., Antimicrob Agents Chemother 62(3), 2018
PMID: 29311062
The global distribution and spread of the mobilized colistin resistance gene mcr-1.
Wang R, van Dorp L, Shaw LP, Bradley P, Wang Q, Wang X, Jin L, Zhang Q, Liu Y, Rieux A, Dorai-Schneiders T, Weinert LA, Iqbal Z, Didelot X, Wang H, Balloux F., Nat Commun 9(1), 2018
PMID: 29563494
Monitoring Colistin Resistance in Food Animals, An Urgent Threat.
Liu Y, Liu JH., Expert Rev Anti Infect Ther 16(6), 2018
PMID: 29865881
Mobile Genetic Elements Associated with Antimicrobial Resistance.
Partridge SR, Kwong SM, Firth N, Jensen SO., Clin Microbiol Rev 31(4), 2018
PMID: 30068738
Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant.
Li R, Xie M, Zhang J, Yang Z, Liu L, Liu X, Zheng Z, Chan EW, Chen S., J Antimicrob Chemother 72(2), 2017
PMID: 28073961
IncP Plasmid Carrying Colistin Resistance Gene mcr-1 in Klebsiella pneumoniae from Hospital Sewage.
Zhao F, Feng Y, Lü X, McNally A, Zong Z., Antimicrob Agents Chemother 61(2), 2017
PMID: 27895009
Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition.
Sun J, Fang LX, Wu Z, Deng H, Yang RS, Li XP, Li SM, Liao XP, Feng Y, Liu YH., Sci Rep 7(1), 2017
PMID: 28336940
Complete genome sequencing revealed novel genetic contexts of the mcr-1 gene in Escherichia coli strains.
Yu CY, Ang GY, Chong TM, Chin PS, Ngeow YF, Yin WF, Chan KG., J Antimicrob Chemother 72(4), 2017
PMID: 28031273
Analysis of Serial Isolates of mcr-1-Positive Escherichia coli Reveals a Highly Active ISApl1 Transposon.
Snesrud E, Ong AC, Corey B, Kwak YI, Clifford R, Gleeson T, Wood S, Whitman TJ, Lesho EP, Hinkle M, McGann P., Antimicrob Agents Chemother 61(5), 2017
PMID: 28223389
Moraxella Species as Potential Sources of MCR-Like Polymyxin Resistance Determinants.
Kieffer N, Nordmann P, Poirel L., Antimicrob Agents Chemother 61(6), 2017
PMID: 28320720
Study of mcr-1 Gene-Mediated Colistin Resistance in Enterobacteriaceae Isolated from Humans and Animals in Different Countries.
Hadjadj L, Riziki T, Zhu Y, Li J, Diene SM, Rolain JM., Genes (Basel) 8(12), 2017
PMID: 29257080
Complete Nucleotide Sequence of an IncI2 Plasmid Coharboring blaCTX-M-55 and mcr-1.
Sun J, Li XP, Yang RS, Fang LX, Huo W, Li SM, Jiang P, Liao XP, Liu YH., Antimicrob Agents Chemother 60(8), 2016
PMID: 27216063
A Model for Transposition of the Colistin Resistance Gene mcr-1 by ISApl1.
Snesrud E, He S, Chandler M, Dekker JP, Hickman AB, McGann P, Dyda F., Antimicrob Agents Chemother 60(11), 2016
PMID: 27620479
Novel blaROB-1-bearing plasmid conferring resistance to β-lactams in Haemophilus parasuis isolates from healthy weaning pigs.
Moleres J, Santos-López A, Lázaro I, Labairu J, Prat C, Ardanuy C, González-Zorn B, Aragon V, Garmendia J., Appl Environ Microbiol 81(9), 2015
PMID: 25747001
A BOX-SCAR fragment for the identification of Actinobacillus pleuropneumoniae.
Rossi CC, Pereira MF, Langford PR, Bazzolli DM., FEMS Microbiol Lett 352(1), 2014
PMID: 24372642
Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.
Bossé JT, Li Y, Angen Ø, Weinert LA, Chaudhuri RR, Holden MT, Williamson SM, Maskell DJ, Tucker AW, Wren BW, Rycroft AN, Langford PR, BRaDP1T consortium., J Clin Microbiol 52(7), 2014
PMID: 24759717
The genetic analysis of the flp locus of Actinobacillus pleuropneumoniae.
Li T, Xu Z, Zhang T, Li L, Chen H, Zhou R., Arch Microbiol 194(3), 2012
PMID: 21847536
ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: analysis of the regions that comprise 12 antimicrobial resistance genes.
Michael GB, Kadlec K, Sweeney MT, Brzuszkiewicz E, Liesegang H, Daniel R, Murray RW, Watts JL, Schwarz S., J Antimicrob Chemother 67(1), 2012
PMID: 22001175
Haemophilus influenzae clinical isolates with plasmid pB1000 bearing blaROB-1: fitness cost and interspecies dissemination.
San Millan A, Garcia-Cobos S, Escudero JA, Hidalgo L, Gutierrez B, Carrilero L, Campos J, Gonzalez-Zorn B., Antimicrob Agents Chemother 54(4), 2010
PMID: 20086141
Population-based analysis of Actinobacillus pleuropneumoniae ApxIVA for use as a DIVA antigen.
O'Neilla C, Jones SCP, Bossé JT, Watson CM, Williamson SM, Rycroft AN, Simon Kroll J, Hartley HM, Langford PR., Vaccine 28(31), 2010
PMID: 20483193
Screening of strain-specific Actinobacillus pleuropneumoniae genes using a combination method.
Lei L, Du C, Yang P, Xie F, Ou P, Han W, Wang J., J Microbiol Methods 77(2), 2009
PMID: 19318057
Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity.
Vanden Bergh PG, Zecchinon LL, Fett T, Desmecht D., Vet Res 40(4), 2009
PMID: 19356397
Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids.
San Millan A, Escudero JA, Gutierrez B, Hidalgo L, Garcia N, Llagostera M, Dominguez L, Gonzalez-Zorn B., Antimicrob Agents Chemother 53(8), 2009
PMID: 19528282
Multiplex PCR that can distinguish between immunologically cross- reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains.
Zhou L, Jones SC, Angen Ø, Bossé JT, Nash JH, Frey J, Zhou R, Chen HC, Kroll JS, Rycroft AN, Langford PR., J Clin Microbiol 46(2), 2008
PMID: 18094137
Characterization of ISApl1, an insertion element identified from Actinobacillus pleuropneumoniae field isolate in China.
Liu J, Tan C, Li J, Chen H, Xu P, He Q, Bei W, Chen H., Vet Microbiol 132(3-4), 2008
PMID: 18632228

24 References

Daten bereitgestellt von Europe PubMed Central.

Basic local alignment search tool.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ., J. Mol. Biol. 215(3), 1990
PMID: 2231712
Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response.
Baltes N, Tonpitak W, Gerlach GF, Hennig-Pauka I, Hoffmann-Moujahid A, Ganter M, Rothkotter HJ., Infect. Immun. 69(1), 2001
PMID: 11119539
Proposal of a new serovar of Actinobacillus pleuropneumoniae: serovar 15.
Blackall PJ, Klaasen HL, van den Bosch H, Kuhnert P, Frey J., Vet. Microbiol. 84(1-2), 2002
PMID: 11731158
Use of recombinant ApxIV in serodiagnosis of Actinobacillus pleuropneumoniae infections, development and prevalidation of the ApxIV ELISA.
Dreyfus A, Schaller A, Nivollet S, Segers RP, Kobisch M, Mieli L, Soerensen V, Hussy D, Miserez R, Zimmermann W, Inderbitzin F, Frey J., Vet. Microbiol. 99(3-4), 2004
PMID: 15066725
Porcine pleuropneumonia.
Fenwick B, Henry S., J. Am. Vet. Med. Assoc. 204(9), 1994
PMID: 8050966
Insertion sequence 1515 in the ply gene of a type 1 clinical isolate of Streptococcus pneumoniae abolishes pneumolysin expression.
Garnier F, Janapatla RP, Charpentier E, Masson G, Grelaud C, Stach JF, Denis F, Ploy MC., J. Clin. Microbiol. 45(7), 2007
PMID: 17494718
Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.
Haesebrouck F, Chiers K, Van Overbeke I, Ducatelle R., Vet. Microbiol. 58(2-4), 1997
PMID: 9453134
Modulation of cell surface sialic acid expression in Neisseria meningitidis via a transposable genetic element.
Hammerschmidt S, Hilse R, van Putten JP, Gerardy-Schahn R, Unkmeir A, Frosch M., EMBO J. 15(1), 1996
PMID: 8598202
Insertion sequences.
Mahillon J, Chandler M., Microbiol. Mol. Biol. Rev. 62(3), 1998
PMID: 9729608
Serological characterization of Actinobacillus pleuropneumoniae biotype 2 strains isolated from pigs in two Danish herds.
Nielsen R, Andresen LO, Plambeck T, Nielsen JP, Krarup LT, Jorsal SE., Vet. Microbiol. 54(1), 1997
PMID: 9050169
Target specificity of insertion element IS30.
Olasz F, Kiss J, Konig P, Buzas Z, Stalder R, Arber W., Mol. Microbiol. 28(4), 1998
PMID: 9643538
Haemophilus pleuropneumoniae serotyping.
Rosendal S, Boyd DA., J. Clin. Microbiol. 16(5), 1982
PMID: 7153336

Sambrook, 1989
Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae.
Schaller A, Kuhn R, Kuhnert P, Nicolet J, Anderson TJ, MacInnes JI, Segers RP, Frey J., Microbiology (Reading, Engl.) 145 ( Pt 8)(), 1999
PMID: 10463177
ISfinder: the reference centre for bacterial insertion sequences.
Siguier P, Perochon J, Lestrade L, Mahillon J, Chandler M., Nucleic Acids Res. 34(Database issue), 2006
PMID: 16381877


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