Nanofluidic devices for dielectrophoretic mobility shift assays by soft lithography

Viefhues M, Regtmeier J, Anselmetti D (2012)
Journal Of Micromechanics And Microengineering 22(11): 115024.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
We report development and application of 3D structured nano-microfluidic devices that were produced via soft lithography with poly(dimethylsiloxane). The procedure does not rely on hazardous or time-consuming production steps. Here, the nanochannels were created by channel-spanning ridges that reduce the flow height of the microchannel. Several realizations of the ridge layout and nanochannel height are demonstrated, depicting the high potential of this technique. The nanochannels proved to be stable even for width-to-height aspect ratios of 873:1. Additionally, an application of these submicrometer structures is presented with a new technique of a dielectrophoretic mobility shift assay (DEMSA). The DEMSA was used to detect different DNA variants, e.g. protein-DNA-complexes, via a shift in (dielectrophoretically retarded) migration velocities within an array of nanoslits.
Erscheinungsjahr
2012
Zeitschriftentitel
Journal Of Micromechanics And Microengineering
Band
22
Ausgabe
11
Art.-Nr.
115024
ISSN
0960-1317
eISSN
1361-6439
Page URI
https://pub.uni-bielefeld.de/record/2544108

Zitieren

Viefhues M, Regtmeier J, Anselmetti D. Nanofluidic devices for dielectrophoretic mobility shift assays by soft lithography. Journal Of Micromechanics And Microengineering. 2012;22(11): 115024.
Viefhues, M., Regtmeier, J., & Anselmetti, D. (2012). Nanofluidic devices for dielectrophoretic mobility shift assays by soft lithography. Journal Of Micromechanics And Microengineering, 22(11), 115024. doi:10.1088/0960-1317/22/11/115024
Viefhues, M., Regtmeier, J., and Anselmetti, D. (2012). Nanofluidic devices for dielectrophoretic mobility shift assays by soft lithography. Journal Of Micromechanics And Microengineering 22:115024.
Viefhues, M., Regtmeier, J., & Anselmetti, D., 2012. Nanofluidic devices for dielectrophoretic mobility shift assays by soft lithography. Journal Of Micromechanics And Microengineering, 22(11): 115024.
M. Viefhues, J. Regtmeier, and D. Anselmetti, “Nanofluidic devices for dielectrophoretic mobility shift assays by soft lithography”, Journal Of Micromechanics And Microengineering, vol. 22, 2012, : 115024.
Viefhues, M., Regtmeier, J., Anselmetti, D.: Nanofluidic devices for dielectrophoretic mobility shift assays by soft lithography. Journal Of Micromechanics And Microengineering. 22, : 115024 (2012).
Viefhues, Martina, Regtmeier, Jan, and Anselmetti, Dario. “Nanofluidic devices for dielectrophoretic mobility shift assays by soft lithography”. Journal Of Micromechanics And Microengineering 22.11 (2012): 115024.

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