Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance

Ritzefeld M, Sewald N (2012)
Journal of Amino Acids 2012: 1-19.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Several proteins, like transcription factors, bind to certain DNA sequences, thereby regulating biochemical pathways that determine the fate of the corresponding cell. Due to these key positions, it is indispensable to analyze protein-DNA interactions and to identify their mode of action. Surface plasmon resonance is a label-free method that facilitates the elucidation of real-time kinetics of biomolecular interactions. In this article, we focus on this biosensor-based method and provide a detailed guide how SPR can be utilized to study binding of proteins to oligonucleotides. After a description of the physical phenomenon and the instrumental realization including fiber-optic-based SPR and SPR imaging, we will continue with a survey of immobilization methods. Subsequently, we will focus on the optimization of the experiment, expose pitfalls, and introduce how data should be analyzed and published. Finally, we summarize several interesting publications of the last decades dealing with protein-DNA and RNA interaction analysis by SPR.
Journal of Amino Acids
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Ritzefeld M, Sewald N. Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of Amino Acids. 2012;2012:1-19.
Ritzefeld, M., & Sewald, N. (2012). Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of Amino Acids, 2012, 1-19. doi:10.1155/2012/816032
Ritzefeld, Markus, and Sewald, Norbert. 2012. “Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance”. Journal of Amino Acids 2012: 1-19.
Ritzefeld, M., and Sewald, N. (2012). Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of Amino Acids 2012, 1-19.
Ritzefeld, M., & Sewald, N., 2012. Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of Amino Acids, 2012, p 1-19.
M. Ritzefeld and N. Sewald, “Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance”, Journal of Amino Acids, vol. 2012, 2012, pp. 1-19.
Ritzefeld, M., Sewald, N.: Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of Amino Acids. 2012, 1-19 (2012).
Ritzefeld, Markus, and Sewald, Norbert. “Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance”. Journal of Amino Acids 2012 (2012): 1-19.

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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