The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection

Savidor A, Teper D, Gartemann K-H, Eichenlaub R, Chalupowicz L, Manulis-Sasson S, Barash I, Tews H, Mayer K, Giannone RJ, Hettich RL, et al. (2012)
Journal of Proteome Research 11(2): 736-750.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Savidor, Alon; Teper, Doron; Gartemann, Karl-HeinzUniBi ; Eichenlaub, RudolfUniBi; Chalupowicz, Laura; Manulis-Sasson, Shulamit; Barash, Isaac; Tews, Helena; Mayer, KerstinUniBi; Giannone, Richard J.; Hettich, Robert L.; Sessa, Guido
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Abstract / Bemerkung
The Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) causes wilt and canker disease of tomato (Solanum lycopersicum). Mechanisms of Cmm pathogenicity and tomato response to Cmm infection are not well understood. To explore the interaction between Cmm and tomato, multidimensional protein identification technology (MudPIT) and tandem mass spectrometry were used to analyze in vitro and in planta generated samples. The results show that during infection Cmm senses the plant environment, transmits signals, induces, and then secretes multiple hydrolytic enzymes, including serine proteases of the Pat-1, Ppa, and Sbt familes, the CelA, XysA, and NagA glycosyl hydrolases, and other cell wall-degrading enzymes. Tomato induction of pathogenesis-related (PR) proteins, LOX1, and other defense-related proteins during infection indicates that the plant senses the invading bacterium and mounts a basal defense response, although partial with some suppressed components including class III peroxidases and a secreted serine peptidase. The tomato ethylene-synthesizing enzyme ACC-oxidase was induced during infection with the wild-type Cmm but not during infection with an endophytic Cmm strain, identifying Cmm-triggered host synthesis of ethylene as an important factor in disease symptom development. The proteomic data were also used to improve Cmm genome annotation, and thousands of Cmm gene models were confirmed or expanded.
Stichworte
secretion; mass spectrometry; technology (MudPIT); multidimensional protein identification; proteomics; michiganensis (Cmm); Solanum lycopersicum (tomato); Clavibacter michiganensis subsp; annotation; comparative analysis; interactome; NSAF
Erscheinungsjahr
2012
Zeitschriftentitel
Journal of Proteome Research
Band
11
Ausgabe
2
Seite(n)
736-750
ISSN
1535-3893
eISSN
1535-3907
Page URI
https://pub.uni-bielefeld.de/record/2489391

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Savidor A, Teper D, Gartemann K-H, et al. The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection. Journal of Proteome Research. 2012;11(2):736-750.
Savidor, A., Teper, D., Gartemann, K. - H., Eichenlaub, R., Chalupowicz, L., Manulis-Sasson, S., Barash, I., et al. (2012). The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection. Journal of Proteome Research, 11(2), 736-750. doi:10.1021/pr200646a
Savidor, Alon, Teper, Doron, Gartemann, Karl-Heinz, Eichenlaub, Rudolf, Chalupowicz, Laura, Manulis-Sasson, Shulamit, Barash, Isaac, et al. 2012. “The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection”. Journal of Proteome Research 11 (2): 736-750.
Savidor, A., Teper, D., Gartemann, K. - H., Eichenlaub, R., Chalupowicz, L., Manulis-Sasson, S., Barash, I., Tews, H., Mayer, K., Giannone, R. J., et al. (2012). The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection. Journal of Proteome Research 11, 736-750.
Savidor, A., et al., 2012. The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection. Journal of Proteome Research, 11(2), p 736-750.
A. Savidor, et al., “The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection”, Journal of Proteome Research, vol. 11, 2012, pp. 736-750.
Savidor, A., Teper, D., Gartemann, K.-H., Eichenlaub, R., Chalupowicz, L., Manulis-Sasson, S., Barash, I., Tews, H., Mayer, K., Giannone, R.J., Hettich, R.L., Sessa, G.: The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection. Journal of Proteome Research. 11, 736-750 (2012).
Savidor, Alon, Teper, Doron, Gartemann, Karl-Heinz, Eichenlaub, Rudolf, Chalupowicz, Laura, Manulis-Sasson, Shulamit, Barash, Isaac, Tews, Helena, Mayer, Kerstin, Giannone, Richard J., Hettich, Robert L., and Sessa, Guido. “The Clavibacter michiganensis subsp michiganensis-Tomato Interactome Reveals the Perception of Pathogen by the Host and Suggests Mechanisms of Infection”. Journal of Proteome Research 11.2 (2012): 736-750.

14 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Plant-like bacterial expansins play contrasting roles in two tomato vascular pathogens.
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