Hidden Multiple Bond Effects in Dynamic Force Spectroscopy

Getfert S, Reimann P (2012)
Biophysical Journal 102(5): 1184-1193.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
In dynamic force spectroscopy, a (bio-) molecular complex is subjected to a steadily increasing force until the chemical bond breaks. Repeating the same experiment many times results in a broad distribution of rupture forces, whose quantitative interpretation represents a formidable theoretical challenge. In this study we address the situation that more than a single molecular bond is involved in one experimental run, giving rise to multiple rupture events that are even more difficult to analyze and thus are usually eliminated as far as possible from the further evaluation of the experimental data. We develop and numerically solve a detailed model of a complete dynamic force spectroscopy experiment including a possible clustering of molecules on the substrate surface, the formation of bonds, their dissociation under load, and the postprocessing of the force extension curves. We show that the data, remaining after elimination of obvious multiple rupture events, may still contain a considerable number of hidden multiple bonds, which are experimentally indistinguishable from true single bonds, but which have considerable effects on the resulting rupture force statistics and its consistent theoretical interpretation.
Biophysical Journal
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Getfert S, Reimann P. Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal. 2012;102(5):1184-1193.
Getfert, S., & Reimann, P. (2012). Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal, 102(5), 1184-1193. doi:10.1016/j.bpj.2012.01.037
Getfert, Sebastian, and Reimann, Peter. 2012. “Hidden Multiple Bond Effects in Dynamic Force Spectroscopy”. Biophysical Journal 102 (5): 1184-1193.
Getfert, S., and Reimann, P. (2012). Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal 102, 1184-1193.
Getfert, S., & Reimann, P., 2012. Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal, 102(5), p 1184-1193.
S. Getfert and P. Reimann, “Hidden Multiple Bond Effects in Dynamic Force Spectroscopy”, Biophysical Journal, vol. 102, 2012, pp. 1184-1193.
Getfert, S., Reimann, P.: Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal. 102, 1184-1193 (2012).
Getfert, Sebastian, and Reimann, Peter. “Hidden Multiple Bond Effects in Dynamic Force Spectroscopy”. Biophysical Journal 102.5 (2012): 1184-1193.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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PMID: 29368695
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Dutta S, Tsiros C, Sundar SL, Athar H, Moore J, Nelson B, Gage MJ, Nishikawa K., Sci Rep 8(1), 2018
PMID: 30275509
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PMID: 22677392

49 References

Daten bereitgestellt von Europe PubMed Central.

Force spectroscopy on single passive biomolecules and single biomolecular bonds
Merkel R.., 2001
Adhesion forces between individual ligand-receptor pairs.
Florin EL, Moy VT, Gaub HE., Science 264(5157), 1994
PMID: 8153628
Specific antigen/antibody interactions measured by force microscopy.
Dammer U, Hegner M, Anselmetti D, Wagner P, Dreier M, Huber W, Guntherodt HJ., Biophys. J. 70(5), 1996
PMID: 9172770
Detection and localization of individual antibody-antigen recognition events by atomic force microscopy.
Hinterdorfer P, Baumgartner W, Gruber HJ, Schilcher K, Schindler H., Proc. Natl. Acad. Sci. U.S.A. 93(8), 1996
PMID: 8622961
Single-molecule experiments in synthetic biology: an approach to the affinity ranking of DNA-binding peptides.
Eckel R, Wilking SD, Becker A, Sewald N, Ros R, Anselmetti D., Angew. Chem. Int. Ed. Engl. 44(25), 2005
PMID: 15906400
Single-molecule force spectroscopy of supramolecular heterodimeric capsules.
Schroder T, Geisler T, Walhorn V, Schnatwinkel B, Anselmetti D, Mattay J., Phys Chem Chem Phys 12(36), 2010
PMID: 20661519
Dynamic strength of molecular adhesion bonds.
Evans E, Ritchie K., Biophys. J. 72(4), 1997
PMID: 9083660
Models for the specific adhesion of cells to cells.
Bell GI., Science 200(4342), 1978
PMID: 347575
Kinetics from nonequilibrium single-molecule pulling experiments.
Hummer G, Szabo A., Biophys. J. 85(1), 2003
PMID: 12829459
Breaking bonds in the atomic force microscope: theory and analysis.
Hanke F, Kreuzer HJ., Phys Rev E Stat Nonlin Soft Matter Phys 74(3 Pt 1), 2006
PMID: 17025669
Intrinsic rates and activation free energies from single-molecule pulling experiments.
Dudko OK, Hummer G, Szabo A., Phys. Rev. Lett. 96(10), 2006
PMID: 16605793
Dynamic response of adhesion complexes: beyond the single-path picture
Bartolo D., Derényi I., Ajdari A.., 2002
Dynamic single-molecule force spectroscopy: bond rupture analysis with variable spacer length
Friedsam C., Wehle A.K., Gaub H.E.., 2003
Quantifying the effects of contact duration, loading rate, and approach velocity on P-selectin-PSGL-1 interactions using AFM
Lü S., Ye Z., Long M.., 2006
Dissociation kinetics of an enzyme-inhibitor system using single-molecule force measurements.
Mayyas E, Bernardo M, Runyan L, Sohail A, Subba-Rao V, Pantea M, Fridman R, Hoffmann PM., Biomacromolecules 11(12), 2010
PMID: 20973496
Kinetic barriers in RNA unzipping
Imparato A., Peliti L.., 2004
Theoretical analysis of single-molecule force spectroscopy experiments: heterogeneity of chemical bonds.
Raible M, Evstigneev M, Bartels FW, Eckel R, Nguyen-Duong M, Merkel R, Ros R, Anselmetti D, Reimann P., Biophys. J. 90(11), 2006
PMID: 16513778
Adhesion clusters under shared linear loading: a stochastic analysis
Erdmann T., Schwarz U.S.., 2004
Impact of receptor-ligand distance on adhesion cluster stability.
Erdmann T, Schwarz US., Eur Phys J E Soft Matter 22(2), 2007
PMID: 17347787
Extracting kinetics from single-molecule force spectroscopy: nanopore unzipping of DNA hairpins.
Dudko OK, Mathe J, Szabo A, Meller A, Hummer G., Biophys. J. 92(12), 2007
PMID: 17384066
Theory, analysis, and interpretation of single-molecule force spectroscopy experiments.
Dudko OK, Hummer G, Szabo A., Proc. Natl. Acad. Sci. U.S.A. 105(41), 2008
PMID: 18852468
Atomic force microscopy reveals the mechanical design of a modular protein.
Li H, Oberhauser AF, Fowler SB, Clarke J, Fernandez JM., Proc. Natl. Acad. Sci. U.S.A. 97(12), 2000
PMID: 10823913
Hidden complexity in the mechanical properties of titin.
Williams PM, Fowler SB, Best RB, Toca-Herrera JL, Scott KA, Steward A, Clarke J., Nature 422(6930), 2003
PMID: 12660787
Dynamic force spectroscopy of protein-DNA interactions by unzipping DNA.
Koch SJ, Wang MD., Phys. Rev. Lett. 91(2), 2003
PMID: 12906513
Anisotropic deformation response of single protein molecules.
Dietz H, Berkemeier F, Bertz M, Rief M., Proc. Natl. Acad. Sci. U.S.A. 103(34), 2006
PMID: 16908850
Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy.
Morfill J, Blank K, Zahnd C, Luginbuhl B, Kuhner F, Gottschalk KE, Pluckthun A, Gaub HE., Biophys. J. 93(10), 2007
PMID: 17675348
Rupture of multiple parallel molecular bonds under dynamic loading.
Seifert U., Phys. Rev. Lett. 84(12), 2000
PMID: 11017316
Multi-bead-and-spring model to interpret protein detachment studied by AFM force spectroscopy.
Gergely C, Hemmerle J, Schaaf P, Horber JK, Voegel JC, Senger B., Biophys. J. 83(2), 2002
PMID: 12124258
Analytical descriptions of dynamic force spectroscopy: behavior of multiple connections
Williams P.M.., 2003
Nonlinearly additive forces in multivalent ligand binding to a single protein revealed with force spectroscopy.
Ratto TV, Rudd RE, Langry KC, Balhorn RL, McElfresh MW., Langmuir 22(4), 2006
PMID: 16460101
Strength of multiple parallel biological bonds.
Sulchek T, Friddle RW, Noy A., Biophys. J. 90(12), 2006
PMID: 16581843
Dynamic force spectroscopy on multiple bonds: experiments and model
Erdmann T., Pierrat W., Schwarz U.S.., 2008
Multiple receptors involved in human rhinovirus attachment to live cells.
Rankl C, Kienberger F, Wildling L, Wruss J, Gruber HJ, Blaas D, Hinterdorfer P., Proc. Natl. Acad. Sci. U.S.A. 105(46), 2008
PMID: 18997008
Effects of multiple-bond ruptures in force spectroscopy measurements of interactions between fullerene C molecules in water
Gu C., Kirkpatrick A., Ray C., Akhremitchev B.B.., 2008
Impact of polymer tether length on multiple ligand-receptor bond formation.
Jeppesen C, Wong JY, Kuhl TL, Israelachvili JN, Mullah N, Zalipsky S, Marques CM., Science 293(5529), 2001
PMID: 11463908
Association kinetics from single molecule force spectroscopy measurements.
Guo S, Lad N, Ray C, Akhremitchev BB., Biophys. J. 96(8), 2009
PMID: 19383484
Scaling exponent and Kuhn length of pinned polymers by single molecule force spectroscopy.
Kuhner F, Erdmann M, Gaub HE., Phys. Rev. Lett. 97(21), 2006
PMID: 17155778
Exact stochastic simulation of coupled chemical reactions
Gillespie D.T.., 1977
Configurations of polymers attached to probes
Bubis R., Kantor Y., Kardar M.., 2009
Detecting molecular fingerprints in single molecule force spectroscopy using pattern recognition
Dietz H., Rief M.., 2007
Measuring two-dimensional receptor-ligand binding kinetics by micropipette.
Chesla SE, Selvaraj P, Zhu C., Biophys. J. 75(3), 1998
PMID: 9726957

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