Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells
Mazur L, Opydo-Chanek M, Wojcieszek K, Stojak M, Niemeyer, IIT-GmbH U (2012)
Folia Biologica 60(1): 35-40.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Mazur, Lidia;
Opydo-Chanek, Malgorzata;
Wojcieszek, Katarzyna;
Stojak, Marta;
Niemeyer, IIT-GmbH, UlfUniBi
Einrichtung
Abstract / Bemerkung
Mafosfamide cyclohexylamine salt (D-17272), 4-hydro-peroxy-cyclophosphamide (D-18864), and beta-D-glucose-isophosphoramide mustard (D-I 9575, glufosfamide) are three new generation oxazaphosphorine agents. The aim of the present study was to compare the cell response to the action of these three oxazaphosphorines. The experiments were performed in vitro on human acute myeloblastic leukemia ML-1 cells. After exposure of ML-1 cells to the oxazaphosphorines, the size, viability and count of these cells were determined. The research was conducted using the spectrophotometric MTT assay and the electronic Beckman Coulter method. The temporary changes in the ML-1 cell size, viability and count, were dependent on the oxazaphosphorine agent tested, its dose, and the time intervals after its application. Among the three oxazaphosphorine agents, D-18864 proved to be the most cytotoxic, and D-19575 was characterized by the lowest cytotoxicity. The results suggest the possibility of using the electronic sizing and counting method and the MTT assay as a rapid in vitro test for assessing leukemic cell sensitivity to the action of new potential chemotherapeutic agents.
Stichworte
viability;
count;
oxazaphosphorines;
Human leukemic ML-1 cells;
cell size;
cytotoxicity
Erscheinungsjahr
2012
Zeitschriftentitel
Folia Biologica
Band
60
Ausgabe
1
Seite(n)
35-40
ISSN
0015-5497
eISSN
1734-9168
Page URI
https://pub.uni-bielefeld.de/record/2474488
Zitieren
Mazur L, Opydo-Chanek M, Wojcieszek K, Stojak M, Niemeyer, IIT-GmbH U. Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica. 2012;60(1):35-40.
Mazur, L., Opydo-Chanek, M., Wojcieszek, K., Stojak, M., & Niemeyer, IIT-GmbH, U. (2012). Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica, 60(1), 35-40. doi:10.3409/fb60_1-2.35-40
Mazur, Lidia, Opydo-Chanek, Malgorzata, Wojcieszek, Katarzyna, Stojak, Marta, and Niemeyer, IIT-GmbH, Ulf. 2012. “Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells”. Folia Biologica 60 (1): 35-40.
Mazur, L., Opydo-Chanek, M., Wojcieszek, K., Stojak, M., and Niemeyer, IIT-GmbH, U. (2012). Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica 60, 35-40.
Mazur, L., et al., 2012. Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica, 60(1), p 35-40.
L. Mazur, et al., “Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells”, Folia Biologica, vol. 60, 2012, pp. 35-40.
Mazur, L., Opydo-Chanek, M., Wojcieszek, K., Stojak, M., Niemeyer, IIT-GmbH, U.: Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica. 60, 35-40 (2012).
Mazur, Lidia, Opydo-Chanek, Malgorzata, Wojcieszek, Katarzyna, Stojak, Marta, and Niemeyer, IIT-GmbH, Ulf. “Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells”. Folia Biologica 60.1 (2012): 35-40.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
1 Zitation in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
The chemomodulatory effects of glufosfamide on docetaxel cytotoxicity in prostate cancer cells.
Attia RT, Tolba MF, Trivedi R, Tadros MG, Arafa HM, Abdel-Naim AB., PeerJ 4(), 2016
PMID: 27413637
Attia RT, Tolba MF, Trivedi R, Tadros MG, Arafa HM, Abdel-Naim AB., PeerJ 4(), 2016
PMID: 27413637
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