Novel platform for DNA alignment and studying DNA-protein interaction in realtime

Venkatesh A (2011)
Bielefeld: Universität.

Bielefelder E-Dissertation| Englisch
 
Download
OA
Betreuer
Reiss, Günter
Abstract / Bemerkung
Recreating DNA-protein interaction in vitro could unravel the relationship between their structure and function, which necessarily requires transforming the DNA from its coiled state to a linear form. In this work, two main techniques were developed to realize this aim namely, orientation-defined DNA alignment and realtime monitoring of polymerase movement. Orientation-defined alignment of DNA was performed by bi- functionalization of DNA with two different pairs of chemical groups namely, thiol and biotin or thiol and silane at the termini. The terminal functionalized DNA was dropped on a nano-fabricated electrode and aligned across the gold and silicon dioxide surface using electrokinetic force. The Movement of polymerase in realtime was monitored us- ing molecular-beacons as reporters. The molecular beacons bind at regular intervals on a DNA that contains highly repeated sequences called homopolymer DNA. The homopolymer DNA will act as a template for the polymerase. The total fluorescence intensity decreases as the polymerase copies the second strand of DNA, due to removal and closing of molecular beacons. E. coli DNA polymerase I, T7 DNA polymerase, T7 RNA polymerase, and N4 virion RNA polymerase were used to validate this new technique.
Stichworte
DNA alignment; dielectrophoresis; DNA stretching
Jahr
2011
Seite(n)
117
Page URI
https://pub.uni-bielefeld.de/record/2472968

Zitieren

Venkatesh A. Novel platform for DNA alignment and studying DNA-protein interaction in realtime. Bielefeld: Universität; 2011.
Venkatesh, A. (2011). Novel platform for DNA alignment and studying DNA-protein interaction in realtime. Bielefeld: Universität.
Venkatesh, A. (2011). Novel platform for DNA alignment and studying DNA-protein interaction in realtime. Bielefeld: Universität.
Venkatesh, A., 2011. Novel platform for DNA alignment and studying DNA-protein interaction in realtime, Bielefeld: Universität.
A. Venkatesh, Novel platform for DNA alignment and studying DNA-protein interaction in realtime, Bielefeld: Universität, 2011.
Venkatesh, A.: Novel platform for DNA alignment and studying DNA-protein interaction in realtime. Universität, Bielefeld (2011).
Venkatesh, Alagarswamy. Novel platform for DNA alignment and studying DNA-protein interaction in realtime. Bielefeld: Universität, 2011.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T09:17:59Z
MD5 Prüfsumme
770dcd0037dbecbf3de7f416a3e926c7

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Suchen in

Google Scholar