Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins α(V)β (3) and α (5)β (1)

Conradi J, Huber S, Gaus K, Mertink F, Royo Gracia S, Strijowski U, Backert S, Sewald N (2012)
Amino Acids 43(1): 219-232.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Conradi, JensUniBi; Huber, Sylwia; Gaus, Katharina; Mertink, FelixUniBi; Royo Gracia, Soledad; Strijowski, Ulf; Backert, Steffen; Sewald, NorbertUniBi
Abstract / Bemerkung
The human pathogen Helicobacter pylori that may cause different gastric diseases exploits integrins for infection of gastric cells. The H. pylori protein CagL present on the outer region of the type IV secretion pilus contains an RGD sequence (-Arg-Gly-Asp-) that enables binding to cells presenting integrins α(5)β(1) and α(V)β(3). This interaction can be inhibited with conformationally designed cyclic RGD peptides derived from the CagL epitope -Ala-Leu-Arg-Gly-Asp-Leu-Ala-. The inhibition of the CagL-α(V)β(3) interaction by different RGD peptides strongly suggests the importance of the RGD motif for CagL binding. CagL point mutants (RAD, RGA) show decreased affinity to integrin α(V)β(3). Furthermore, structure-activity relationship studies with cyclic RGD peptides in a spatial screening approach show the distinct influence of the three-dimensional arrangement of RGD motif on the ability to interfere with this interaction. Resulting from these studies, similar structural requirements for the CagL epitope as previously suggested for other ligands of integrin α(V)β(3) are proposed.
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Conradi J, Huber S, Gaus K, et al. Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins α(V)β (3) and α (5)β (1). Amino Acids. 2012;43(1):219-232.
Conradi, J., Huber, S., Gaus, K., Mertink, F., Royo Gracia, S., Strijowski, U., Backert, S., et al. (2012). Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins α(V)β (3) and α (5)β (1). Amino Acids, 43(1), 219-232. doi:10.1007/s00726-011-1066-0
Conradi, J., Huber, S., Gaus, K., Mertink, F., Royo Gracia, S., Strijowski, U., Backert, S., and Sewald, N. (2012). Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins α(V)β (3) and α (5)β (1). Amino Acids 43, 219-232.
Conradi, J., et al., 2012. Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins α(V)β (3) and α (5)β (1). Amino Acids, 43(1), p 219-232.
J. Conradi, et al., “Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins α(V)β (3) and α (5)β (1)”, Amino Acids, vol. 43, 2012, pp. 219-232.
Conradi, J., Huber, S., Gaus, K., Mertink, F., Royo Gracia, S., Strijowski, U., Backert, S., Sewald, N.: Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins α(V)β (3) and α (5)β (1). Amino Acids. 43, 219-232 (2012).
Conradi, Jens, Huber, Sylwia, Gaus, Katharina, Mertink, Felix, Royo Gracia, Soledad, Strijowski, Ulf, Backert, Steffen, and Sewald, Norbert. “Cyclic RGD peptides interfere with binding of the Helicobacter pylori protein CagL to integrins α(V)β (3) and α (5)β (1)”. Amino Acids 43.1 (2012): 219-232.

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