Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge

Luesken FA, van Alen TA, van der Biezen E, Frijters C, Toonen G, Kampman C, Hendrickx TLG, Zeeman G, Temmink H, Strous M, den Camp HJMO, et al. (2011)
Applied Microbiology and Biotechnology 92(4): 845-854.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
Luesken, Francisca A.; van Alen, Theo A.; van der Biezen, Erwin; Frijters, Carla; Toonen, Ger; Kampman, Christel; Hendrickx, Tim L. G.; Zeeman, Grietje; Temmink, Hardy; Strous, MarcUniBi; den Camp, Huub J. M. Op; Jetten, Mike S. M.
Alle
Abstract / Bemerkung
Recently discovered microorganisms affiliated to the bacterial phylum NC10, named "Candidatus Methylomirabilis oxyfera", perform nitrite-dependent anaerobic methane oxidation. These microorganisms could be important players in a novel way of anaerobic wastewater treatment where ammonium and residual dissolved methane might be removed at the expense of nitrate or nitrite. To find suitable inocula for reactor startup, ten selected wastewater treatment plants (WWTPs) located in The Netherlands were screened for the endogenous presence of M. oxyfera using molecular diagnostic methods. We could identify NC10 bacteria with 98% similarity to M. oxyfera in nine out of ten WWTPs tested. Sludge from one selected WWTP was used to start a new enrichment culture of NC10 bacteria. This enrichment was monitored using specific pmoA primers and M. oxyfera cells were visualized with fluorescence oligonucleotide probes. After 112 days, the enrichment consumed up to 0.4 mM NO(2)(-) per day. The results of this study show that appropriate sources of biomass, enrichment strategies, and diagnostic tools existed to start and monitor pilot scale tests for the implementation of nitrite-dependent methane oxidation in wastewater treatment at ambient temperature.
Stichworte
Anaerobic oxidation; pmoA primers; Anaerobic wastewater treatment; Nitrogen removal; Denitrification; Methanotroph; Methylomirabilis oxyfera; NC10 phylum
Erscheinungsjahr
2011
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
92
Ausgabe
4
Seite(n)
845-854
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/2440575

Zitieren

Luesken FA, van Alen TA, van der Biezen E, et al. Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge. Applied Microbiology and Biotechnology. 2011;92(4):845-854.
Luesken, F. A., van Alen, T. A., van der Biezen, E., Frijters, C., Toonen, G., Kampman, C., Hendrickx, T. L. G., et al. (2011). Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge. Applied Microbiology and Biotechnology, 92(4), 845-854. doi:10.1007/s00253-011-3361-9
Luesken, F. A., van Alen, T. A., van der Biezen, E., Frijters, C., Toonen, G., Kampman, C., Hendrickx, T. L. G., Zeeman, G., Temmink, H., Strous, M., et al. (2011). Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge. Applied Microbiology and Biotechnology 92, 845-854.
Luesken, F.A., et al., 2011. Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge. Applied Microbiology and Biotechnology, 92(4), p 845-854.
F.A. Luesken, et al., “Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge”, Applied Microbiology and Biotechnology, vol. 92, 2011, pp. 845-854.
Luesken, F.A., van Alen, T.A., van der Biezen, E., Frijters, C., Toonen, G., Kampman, C., Hendrickx, T.L.G., Zeeman, G., Temmink, H., Strous, M., den Camp, H.J.M.O., Jetten, M.S.M.: Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge. Applied Microbiology and Biotechnology. 92, 845-854 (2011).
Luesken, Francisca A., van Alen, Theo A., van der Biezen, Erwin, Frijters, Carla, Toonen, Ger, Kampman, Christel, Hendrickx, Tim L. G., Zeeman, Grietje, Temmink, Hardy, Strous, Marc, den Camp, Huub J. M. Op, and Jetten, Mike S. M. “Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge”. Applied Microbiology and Biotechnology 92.4 (2011): 845-854.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 21667086
PubMed | Europe PMC

Suchen in

Google Scholar