Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography

Staiger D, Becker F, Schell J, Koncz C, Palme K (1991)
European Journal of Biochemistry 199(3): 519-527.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
DOI
Autor*in
Staiger, DorotheeUniBi; Becker, Frank; Schell, Jeff; Koncz, Csaba; Palme, Klaus
Einrichtung
Fakultät für Biologie > RNA Biologie und Molekulare Physiologie
Abstract / Bemerkung
The activity of various light-regulated and developmentally regulated plant gene promoters critically depends upon the presence of a conserved sequence with a central CACGTG motif. Using band-shift assays, we have identified nuclear factor(s) from Nicotiana tabacum, termed CG-1, that specifically recognize(s) this transcriptional element in the ultraviolet-light-regulated Antirrhinum majus chalcone synthase promoter. CG-1 activity is constitutively expressed in tobacco seedlings grown in the absence of ultraviolet light as well as in seedlings induced for chalcone synthase gene expression by ultraviolet light irradiation. CG-1 activity has also been detected in flower tissue. DNA-protein cross-linking studies identified three polypeptides with apparent molecular masses of 20, 32 and 42 kDa binding to the CACGTG motif. Proteins interacting with the CACGTG motif were purified from N. tabacum seedlings using differential sequence-specific DNA affinity chromatography employing wild-type and mutated CG-1-binding sites. Denaturing polyacrylamide gel electrophoresis revealed major polypeptides of approximately 20, 30 and 40 kDa which are highly enriched in the affinity-purified fractions binding specifically to the CACGTG motif.
Erscheinungsjahr
1991
Zeitschriftentitel
European Journal of Biochemistry
Band
199
Ausgabe
3
Seite(n)
519-527
ISSN
0014-2956
eISSN
1432-1033
Page URI
https://pub.uni-bielefeld.de/record/2439191

Zitieren

Staiger D, Becker F, Schell J, Koncz C, Palme K. Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography. European Journal of Biochemistry. 1991;199(3):519-527.
Staiger, D., Becker, F., Schell, J., Koncz, C., & Palme, K. (1991). Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography. European Journal of Biochemistry, 199(3), 519-527. https://doi.org/10.1111/j.1432-1033.1991.tb16150.x
Staiger, Dorothee, Becker, Frank, Schell, Jeff, Koncz, Csaba, and Palme, Klaus. 1991. “Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography”. European Journal of Biochemistry 199 (3): 519-527.
Staiger, D., Becker, F., Schell, J., Koncz, C., and Palme, K. (1991). Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography. European Journal of Biochemistry 199, 519-527.
Staiger, D., et al., 1991. Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography. European Journal of Biochemistry, 199(3), p 519-527.
D. Staiger, et al., “Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography”, European Journal of Biochemistry, vol. 199, 1991, pp. 519-527.
Staiger, D., Becker, F., Schell, J., Koncz, C., Palme, K.: Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography. European Journal of Biochemistry. 199, 519-527 (1991).
Staiger, Dorothee, Becker, Frank, Schell, Jeff, Koncz, Csaba, and Palme, Klaus. “Purification of tobacco nuclear proteins binding to a CACGTG motif of the chalcone synthase promoter by DNA affinity chromatography”. European Journal of Biochemistry 199.3 (1991): 519-527.

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Computational exploration of cis-regulatory modules in rhythmic expression data using the "Exploration of Distinctive CREs and CRMs" (EDCC) and "CRM Network Generator" (CNG) programs.
Bekiaris PS, Tekath T, Staiger D, Danisman S., PLoS One 13(1), 2018
PMID: 29298348
Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation.
Zhu Z, Wang H, Wang Y, Guan S, Wang F, Tang J, Zhang R, Xie L, Lu Y., J Exp Bot 66(13), 2015
PMID: 25911741
Methods for transcription factor separation.
Moxley RA, Jarrett HW, Mitra S., J Chromatogr B Analyt Technol Biomed Life Sci 797(1-2), 2003
PMID: 14630155
Two bZIP proteins from Antirrhinum flowers preferentially bind a hybrid C-box/G-box motif and help to define a new sub-family of bZIP transcription factors.
Martínez-García JF, Moyano E, Alcocer MJ, Martin C., Plant J 13(4), 1998
PMID: 9680995
Cis elements and potential trans-acting factors for the developmental regulation of the Phaseolus vulgaris CHS15 promoter.
Hotter GS, Kooter J, Dubery IA, Lamb CJ, Dixon RA, Harrison MJ., Plant Mol Biol 28(6), 1995
PMID: 7548834
Transcriptional regulation of environmentally inducible genes in plants by an evolutionary conserved family of G-box binding factors.
de Vetten NC, Ferl RJ., Int J Biochem 26(9), 1994
PMID: 7988731
DNA-protein interactions on a cis-DNA element essential for ethylene regulation.
Meller Y, Sessa G, Eyal Y, Fluhr R., Plant Mol Biol 23(3), 1993
PMID: 8219081
Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins.
Schindler U, Menkens AE, Beckmann H, Ecker JR, Cashmore AR., EMBO J 11(4), 1992
PMID: 1373374
Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding.
Williams ME, Foster R, Chua NH., Plant Cell 4(4), 1992
PMID: 1498606
Homodimeric and heterodimeric leucine zipper proteins and nuclear factors from parsley recognize diverse promoter elements with ACGT cores.
Armstrong GA, Weisshaar B, Hahlbrock K., Plant Cell 4(5), 1992
PMID: 1498607
Two G-box-related sequences confer different expression patterns in transgenic tobacco.
Salinas J, Oeda K, Chua NH., Plant Cell 4(12), 1992
PMID: 1467649

45 References

Daten bereitgestellt von Europe PubMed Central.

Genetic interactions underlying flower color patterns in Antirrhinum majus.
Almeida J, Carpenter R, Robbins TP, Martin C, Coen ES., Genes Dev. 3(11), 1989
PMID: 2558047
Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
Bohmann D, Bos TJ, Admon A, Nishimura T, Vogt PK, Tjian R., Science 238(4832), 1987
PMID: 2825349
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Bradford MM., Anal. Biochem. 72(), 1976
PMID: 942051

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
A liver-specific factor essential for albumin transcription differs between differentiated and dedifferentiated rat hepatoma cells.
Cereghini S, Blumenfeld M, Yaniv M., Genes Dev. 2(8), 1988
PMID: 3169549
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ., Biochemistry 18(24), 1979
PMID: 518835
Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus.
Coen ES, Carpenter R, Martin C., Cell 47(2), 1986
PMID: 3021338

Dangl, Cell Cult. Somatic Cell Genet. Plants 6(), 1989
Characterization of the Arabidopsis Adh G-box binding factor.
DeLisle AJ, Ferl RJ., Plant Cell 2(6), 1990
PMID: 2152176
Mutation of either G box or I box sequences profoundly affects expression from the Arabidopsis rbcS-1A promoter.
Donald RG, Cashmore AR., EMBO J. 9(6), 1990
PMID: 2347304
The plant G box promoter sequence activates transcription in Saccharomyces cerevisiae and is bound in vitro by a yeast activity similar to GBF, the plant G box binding factor.
Donald RG, Schindler U, Batschauer A, Cashmore AR., EMBO J. 9(6), 1990
PMID: 2161333

Drews, J. Cell. Biol. 12(), 1988
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
Fried M, Crothers DM., Nucleic Acids Res. 9(23), 1981
PMID: 6275366
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
Garner MM, Revzin A., Nucleic Acids Res. 9(13), 1981
PMID: 6269071
An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.
Giuliano G, Pichersky E, Malik VS, Timko MP, Scolnik PA, Cashmore AR., Proc. Natl. Acad. Sci. U.S.A. 85(19), 1988
PMID: 2902624

AUTHOR UNKNOWN, 0
Highly purified "flavanone synthase" from parsley catalyzes the formation of naringenin chalcone.
Heller W, Hahlbrock K., Arch. Biochem. Biophys. 200(2), 1980
PMID: 7436427
Affinity purification of sequence-specific DNA binding proteins.
Kadonaga JT, Tjian R., Proc. Natl. Acad. Sci. U.S.A. 83(16), 1986
PMID: 3461465
Light-induced expression of the chimeric chalcone synthase-NPTII gene in tobacco cells.
Kaulen H, Schell J, Kreuzaler F., EMBO J. 5(1), 1986
PMID: 16453661
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Laemmli UK., Nature 227(5259), 1970
PMID: 5432063
Purification of a set of cellular polypeptides that bind to the purine-rich cis-regulatory element of herpes simplex virus immediate early genes.
LaMarco KL, McKnight SL., Genes Dev. 3(9), 1989
PMID: 2558055
Signals and transduction mechanisms for activation of plant defenses against microbial attack.
Lamb CJ, Lawton MA, Dron M, Dixon RA., Cell 56(2), 1989
PMID: 2643475
UV-inducible transient expression in parsley protoplasts identifies regulatory cis-elements of a chimeric Antirrhinum majus chalcone synthase gene.
Lipphardt S, Brettschneider R, Kreuzaler F, Schell J, Dangl JL., EMBO J. 7(13), 1988
PMID: 16453865

Maniatis, 1982
In vivo and in vitro characterization of protein interactions with the dyad G-box of the Arabidopsis Adh gene.
McKendree WL, Paul AL, DeLisle AJ, Ferl RJ., Plant Cell 2(3), 1990
PMID: 2152112
A yeast homolog of the human UEF stimulates transcription from the adenovirus 2 major late promoter in yeast and in mammalian cell-free systems.
Moncollin V, Stalder R, Verdier JM, Sentenac A, Egly JM., Nucleic Acids Res. 18(16), 1990
PMID: 2204028
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
Morrissey JH., Anal. Biochem. 117(2), 1981
PMID: 6172996
A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes.
Peters NK, Frost JW, Long SR., Science 233(4767), 1986
PMID: 3738520
Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro.
Rauscher FJ 3rd, Voulalas PJ, Franza BR Jr, Curran T., Genes Dev. 2(12B), 1988
PMID: 2467839
An ecdysone response element in the Drosophila hsp27 promoter.
Riddihough G, Pelham HR., EMBO J. 6(12), 1987
PMID: 16453813
Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
Sawadogo M, Roeder RG., Cell 43(1), 1985
PMID: 4075392
Photoregulated gene expression may involve ubiquitous DNA binding proteins.
Schindler U, Cashmore AR., EMBO J. 9(11), 1990
PMID: 2209551
In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves.
Schmelzer E, Jahnen W, Hahlbrock K., Proc. Natl. Acad. Sci. U.S.A. 85(9), 1988
PMID: 16578833
Inducible in vivo DNA footprints define sequences necessary for UV light activation of the parsley chalcone synthase gene.
Schulze-Lefert P, Dangl JL, Becker-Andre M, Hahlbrock K, Schulz W., EMBO J. 8(3), 1989
PMID: 2566481
Functional architecture of the light-responsive chalcone synthase promoter from parsley.
Schulze-Lefert P, Becker-Andre M, Schulz W, Hahlbrock K, Dangl JL., Plant Cell 1(7), 1989
PMID: 2535519
Purification of intercalator-released p67, a polypeptide that interacts specifically with the c-fos serum response element.
Schroter H, Shaw PE, Nordheim A., Nucleic Acids Res. 15(24), 1987
PMID: 3320962
Positive and negative regulation of the gene for transcription factor IIIA in Xenopus laevis oocytes.
Scotto KW, Kaulen H, Roeder RG., Genes Dev. 3(5), 1989
PMID: 2744458

AUTHOR UNKNOWN, 0
Purification and characterization of a heat-shock element binding protein from yeast.
Sorger PK, Pelham HR., EMBO J. 6(10), 1987
PMID: 3319580

Staiger, Proc. Natl Acad. Sci. USA 86(), 1989
A Nuclear Factor Recognizing a Positive Regulatory Upstream Element of the Antirrhinum majus Chalcone Synthase Promoter.
Staiger D, Kaulen H, Schell J., Plant Physiol. 93(4), 1990
PMID: 16667623

AUTHOR UNKNOWN, 0
Promoter analysis of the chalcone synthase (chsA) gene of Petunia hybrida: a 67 bp promoter region directs flower-specific expression.
van der Meer IM, Spelt CE, Mol JN, Stuitje AR., Plant Mol. Biol. 15(1), 1990
PMID: 2103446
Purification and properties of Drosophila heat shock activator protein.
Wu C, Wilson S, Walker B, Dawid I, Paisley T, Zimarino V, Ueda H., Science 238(4831), 1987
PMID: 3685975
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 1714388
PubMed | Europe PMC

Suchen in

Google Scholar