The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores

Staiger D, Kappeler S, Müller M, Apel K (1994)
Planta 192(2): 221-231.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ;
Abstract / Bemerkung
By differential screening of a copy DNA (cDNA) library from flowering Sinapis alba L. apices against cDNAs from vegetative apices, two cDNA clones were isolated representing transcripts that are expressed transiently at an early stage of tapetum development. The Sa tap35 cDNA encodes a polypeptide with a predicted molecular weight of 12.7 kDa and an isoelectric point of 10.4. The Sa tap44 cDNA codes for a putative 12.4-kDa polypeptide with an isoelectric point of 7.5. The deduced amino-acid sequences display 76% sequence identity and contain an N-terminal stretch of hydrophobic amino acids which has characteristics of secretory signal sequences. In-vitro transcription of the cDNAs and translation of the resulting RNAs in the presence of canine pancreatic microsomes demonstrates that the two proteins are translocated into the microsomes and that the putative preproteins are proteolytically processed to the mature forms. By immunoelectron microscopy the Sa TAP35 and Sa TAP44 proteins were detected at the developing peritapetal membrane between the tapetal cytoplasm and the adjacent middle layer of the anther wall. Furthermore, labelling was observed within the locule in association with globules resembling pro-Ubisch bodies which appeared at the tetrad stage. During the early vacuolate stage of microspore development the young exine was strongly labelled. The exine and the peritapetal membrane both are composed of sporopollenin, and the pro-Ubisch bodies are thought to contain sporopollenin precursors. Thus, Sa TAP35 and Sa TAP44 might be involved in sporopollenin formation and/or deposition.
Erscheinungsjahr
Zeitschriftentitel
Planta
Band
192
Ausgabe
2
Seite(n)
221-231
ISSN
eISSN
PUB-ID

Zitieren

Staiger D, Kappeler S, Müller M, Apel K. The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores. Planta. 1994;192(2):221-231.
Staiger, D., Kappeler, S., Müller, M., & Apel, K. (1994). The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores. Planta, 192(2), 221-231. doi:10.1007/BF01089038
Staiger, D., Kappeler, S., Müller, M., and Apel, K. (1994). The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores. Planta 192, 221-231.
Staiger, D., et al., 1994. The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores. Planta, 192(2), p 221-231.
D. Staiger, et al., “The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores”, Planta, vol. 192, 1994, pp. 221-231.
Staiger, D., Kappeler, S., Müller, M., Apel, K.: The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores. Planta. 192, 221-231 (1994).
Staiger, Dorothee, Kappeler, Stefan, Müller, Martin, and Apel, Klaus. “The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores”. Planta 192.2 (1994): 221-231.

14 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Ontogeny of the Calliandra - massulae (Mimosaceae: Ingeae), and the associated viscin body
Greissl R., Flora; morphologie, geobotanik, oekophysiologie. 201(7), 2006
PMID: IND43867092
Isolation and characterisation of two wheat beta-expansin genes expressed during male gametophyte development.
Jin Y, Tashpulatov AS, Katholnigg H, Heberle-Bors E, Touraev A., Protoplasma 228(1-3), 2006
PMID: 16937050
The classical Ubisch bodies carry a sporophytically produced structural protein (RAFTIN) that is essential for pollen development.
Wang A, Xia Q, Xie W, Datla R, Selvaraj G., Proc Natl Acad Sci U S A 100(24), 2003
PMID: 14612572
Characterization of MZm3-3, a Zea mays tapetum-specific transcript.
Lauga B, Charbonnel-Campaa L, Combes D., Plant Sci 157(1), 2000
PMID: 10940470
Molecular characterization of rice genes specifically expressed in the anther tapetum.
Tsuchiya T, Toriyama K, Ejiri S, Hinata K., Plant Mol Biol 26(6), 1994
PMID: 7858214

53 References

Daten bereitgestellt von Europe PubMed Central.

Immunoprecipitation of proteins from cell-free translations.
Anderson DJ, Blobel G., Meth. Enzymol. 96(), 1983
PMID: 6361451

Antoniew, J. Gen. Virol. 47(), 1980
Nuclear dependency of chloroplast proteins in Acetabularia.
Apel K, Schweiger HG., Eur. J. Biochem. 25(2), 1972
PMID: 5039839

Ausubel, 1987

Bernier, Annu. Rev. Plant Physiol. Plant Mol. Biol. 39(), 1988

Bino, Theor. Appl. Genet. 69(), 1985
Purification of maize pollen exines and analysis of associated proteins.
Chay CH, Buehler EG, Thorn JM, Whelan TM, Bedinger PA., Plant Physiol. 100(2), 1992
PMID: 16653056

Dickinson, New Phytol. 69(), 1970

Echlin, 1971

Erickson, Am. J. Bot. 35(), 1948
Timing of callase activity and cytoplasmic male sterility in Petunia.
Frankel R, Izhar S, Nitsan J., Biochem. Genet. 3(5), 1969
PMID: 5358139
High resolution solid state C NMR spectroscopy of sporopollenins from different plant taxa.
Guilford WJ, Schneider DM, Labovitz J, Opella SJ., Plant Physiol. 86(1), 1988
PMID: 16665854

AUTHOR UNKNOWN, 0

Heslop-Harrison, Can. J. Bot. 47(), 1969

Heslop-Harrison, 1971

Heslop-Harrison, Annu. Rev. Plant Physiol. 26(), 1975

Hess, Planta 189(), 1993

Humbel, Beitr. Elektronenmikroskop. Direktabb. Oberfl. 16(), 1983

Kaul, 1988
Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development.
Koltunow AM, Truettner J, Cox KH, Wallroth M, Goldberg RB., Plant Cell 2(12), 1990
PMID: 12354953

Majewski, 1990

Mariani, Nature 347(), 1990

Mariani, Nature 357(), 1992

Murgia, Sex Plant Reprod. 4(), 1991

Nagahashi, Protoplasma 134(), 1986

Nave, J. Plant Physiol. 125(), 1986
The isolation and characterisation of the tapetum-specific Arabidopsis thaliana A9 gene.
Paul W, Hodge R, Smartt S, Draper J, Scott R., Plant Mol. Biol. 19(4), 1992
PMID: 1627774

Sawhney, J. Plant Physiol. 125(), 1986

Schulze-Osthoff, J. Plant Physiol. 131(), 1987
Patterns of gene expression in developing anthers of Brassica napus.
Scott R, Dagless E, Hodge R, Paul W, Soufleri I, Draper J., Plant Mol. Biol. 17(2), 1991
PMID: 1863773
Identification and characterization of stamen- and tapetum-specific genes from tomato.
Smith AG, Gasser CS, Budelier KA, Fraley RT., Mol. Gen. Genet. 222(1), 1990
PMID: 2233685

Southworth, 1990

AUTHOR UNKNOWN, 0
High pressure freezing comes of age.
Studer D, Michel M, Muller M., Scanning Microsc. Suppl. 3(), 1989
PMID: 2694271
A Brassica S locus gene promoter directs sporophytic expression in the anther tapetum of transgenic Arabidopsis.
Toriyama K, Thorsness MK, Nasrallah JB, Nasrallah ME., Dev. Biol. 143(2), 1991
PMID: 1991565
Patterns of amino acids near signal-sequence cleavage sites.
von Heijne G., Eur. J. Biochem. 133(1), 1983
PMID: 6852022

Heijne, Nucleic Acids Res. 11(), 1986

Wiermann, Int. Rev. Cytol. 140(), 1992

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 7764317
PubMed | Europe PMC

Suchen in

Google Scholar