Phosphate transport and apoplastic phosphate homeostasis in barley leaves

Mimura T, Yin ZH, Wirth E, Dietz K-J (1992)
Plant and Cell Physiology 33(5): 563-568.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Levels of apoplastic inorganic phosphate (P(i)) in leaves and P(i)-transport activities of mesophyll cells were measured in situ in control and P(i)-deficient plants. When detached leaves were fed a solution that contained 10 mM P(i), the apoplastic P(i) levels, as measured by an infiltration method, remained almost constant. When the leaves were immersed in pure water, the apoplastic P(i) level gradually decreased. With 50 mM P(i) in the feeding solution, the level increased dramatically. The apoplastic P(i) levels in P(i)-deficient leaves were somewhat, but not very much lower than those in controls. When the immersion medium was changed to pure water 60 min after feeding with 10 mM P(i), the apoplastic P(i) levels started to decrease and then returned to the initial level. It is suggested that intracellular P(i) may be transported back to the apoplast to maintain the apoplastic P(i) levels at a constant value. Changes in cytoplasmic pH were measured during feeding of P(i) to the leaves by use of the pH-sensitive fluorescent dye, pyranine after Yin et al. (1990a, b). On feeding of P(i), the cytoplasmic pH decreased in P(i)-deficient plants as a result of co-transport of P(i) and protons in situ. After removal of P(i) from the immersion medium, the cytoplasmic pH returned to the original value.
Stichworte
PLASMA MEMBRANE; CYTOPLASMIC PH; APOPLAST; HORDEUM-VULGARE; INORGANIC PHOSPHATE; PHOSPHATE HOMEOSTASIS
Erscheinungsjahr
1992
Zeitschriftentitel
Plant and Cell Physiology
Band
33
Ausgabe
5
Seite(n)
563-568
ISSN
0032-0781
Page URI
https://pub.uni-bielefeld.de/record/2408077

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Mimura T, Yin ZH, Wirth E, Dietz K-J. Phosphate transport and apoplastic phosphate homeostasis in barley leaves. Plant and Cell Physiology. 1992;33(5):563-568.
Mimura, T., Yin, Z. H., Wirth, E., & Dietz, K. - J. (1992). Phosphate transport and apoplastic phosphate homeostasis in barley leaves. Plant and Cell Physiology, 33(5), 563-568.
Mimura, T., Yin, Z. H., Wirth, E., and Dietz, K. - J. (1992). Phosphate transport and apoplastic phosphate homeostasis in barley leaves. Plant and Cell Physiology 33, 563-568.
Mimura, T., et al., 1992. Phosphate transport and apoplastic phosphate homeostasis in barley leaves. Plant and Cell Physiology, 33(5), p 563-568.
T. Mimura, et al., “Phosphate transport and apoplastic phosphate homeostasis in barley leaves”, Plant and Cell Physiology, vol. 33, 1992, pp. 563-568.
Mimura, T., Yin, Z.H., Wirth, E., Dietz, K.-J.: Phosphate transport and apoplastic phosphate homeostasis in barley leaves. Plant and Cell Physiology. 33, 563-568 (1992).
Mimura, T., Yin, Z. H., Wirth, E., and Dietz, Karl-Josef. “Phosphate transport and apoplastic phosphate homeostasis in barley leaves”. Plant and Cell Physiology 33.5 (1992): 563-568.