Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s)

Muller M, Baniahmad C, Kaltschmidt C, Renkawitz R (1991)
Mol Endocrinol 5(10): 1498-503.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Muller, M.; Baniahmad, C.; Kaltschmidt, ChristianUniBi; Renkawitz, R.
Abstract / Bemerkung
Steroid induction of responsive genes functions through the synergistic activity of steroid receptor-binding sequences with adjacent transcription factor-binding sites. To analyze the mechanism of synergy we tested different human glucocorticoid receptor mutants for synergistic function with another transcription factor in comparison with intrinsic trans-activation obtained with a single receptor binding site (glucocorticoid response element). Multiple domains were found to be involved in synergistic activity of the glucocorticoid receptor with the CACCC box factor. Deletions within the N-terminal receptor half affected simultaneously intrinsic trans-activation and synergism. However, deletion of the hormone-binding domain mainly impaired synergism rather than intrinsic trans-activation, clearly showing that this domain synergizes by a mechanism independent of intrinsic activation. A chimeric protein where the DNA-binding domain of the glucocorticoid receptor was replaced by that of the yeast GAL4 protein also showed functional synergism. These data suggest that some of the receptor domains outside the DNA-binding domain synergize by their intrinsic trans-activating property, but the hormone-binding domain contributes to synergism by a different mechanism.
Stichworte
Receptors; Plasmids; Site-Directed; Mutagenesis; Molecular Sequence Data; Kinetics; Genetic Vectors; Fungal Proteins/genetics/metabolism; DNA-Binding Proteins/*metabolism; Chromosome Deletion; Cell Line; Binding Sites; Animals; Base Sequence; Glucocorticoid/genetics/*metabolism; Recombinant Fusion Proteins/metabolism; *Regulatory Sequences; Nucleic Acid; *Saccharomyces cerevisiae Proteins; Transcription Factors/*metabolism
Erscheinungsjahr
1991
Zeitschriftentitel
Mol Endocrinol
Band
5
Ausgabe
10
Seite(n)
1498-503
ISSN
0888-8809 (Print) 0888-8809 (Linking)
Page URI
https://pub.uni-bielefeld.de/record/2398790

Zitieren

Muller M, Baniahmad C, Kaltschmidt C, Renkawitz R. Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s). Mol Endocrinol. 1991;5(10):1498-503.
Muller, M., Baniahmad, C., Kaltschmidt, C., & Renkawitz, R. (1991). Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s). Mol Endocrinol, 5(10), 1498-503.
Muller, M., Baniahmad, C., Kaltschmidt, Christian, and Renkawitz, R. 1991. “Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s)”. Mol Endocrinol 5 (10): 1498-503.
Muller, M., Baniahmad, C., Kaltschmidt, C., and Renkawitz, R. (1991). Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s). Mol Endocrinol 5, 1498-503.
Muller, M., et al., 1991. Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s). Mol Endocrinol, 5(10), p 1498-503.
M. Muller, et al., “Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s)”, Mol Endocrinol, vol. 5, 1991, pp. 1498-503.
Muller, M., Baniahmad, C., Kaltschmidt, C., Renkawitz, R.: Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s). Mol Endocrinol. 5, 1498-503 (1991).
Muller, M., Baniahmad, C., Kaltschmidt, Christian, and Renkawitz, R. “Multiple domains of the glucocorticoid receptor involved in synergism with the CACCC box factor(s)”. Mol Endocrinol 5.10 (1991): 1498-503.

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