The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night

Staiger D, Apel K, Trepp G (1999)
Plant Molecular Biology 40(5): 873-882.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Staiger, DorotheeUniBi; Apel, Klaus; Trepp, Gian
Abstract / Bemerkung
In Arabidopsis thaliana, steady-state abundance of the Atger3 transcript encoding a germin-like cell wall protein follows a circadian rhythm, reaching its highest level at the beginning of the night. As a first step towards dissecting the molecular mechanisms underlying these transcript oscillations, the Atger3 genomic locus was characterised. Transcriptional fusions of 1.8 kb and 967 bp Atger3 promoter fragments to the beta-glucuronidase (GUS) reporter gene mediate high-amplitude circadian oscillations of the GUS transcript in transgenic Arabidopsis. 5' deletion to -490 greatly reduces overall transcript abundance while retaining a basal oscillation. Further deletion to -299 abolishes preferential GUS expression in the evening. Taken together, these data indicate that clock-response elements contributing to high-amplitude Atger3 oscillations largely reside between -299 and -967. Histochemical staining for GUS activity indicates that the Atger3 promoter is active in cotyledons, young leaves, petioles, the inflorescence axis, pedicels, sepals, ovary, style and siliques but not in roots, petals and anthers.
promoter; germin-like protein; circadian rhythm; Arabidopsis thaliana
Plant Molecular Biology
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Staiger D, Apel K, Trepp G. The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night. Plant Molecular Biology. 1999;40(5):873-882.
Staiger, D., Apel, K., & Trepp, G. (1999). The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night. Plant Molecular Biology, 40(5), 873-882.
Staiger, D., Apel, K., and Trepp, G. (1999). The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night. Plant Molecular Biology 40, 873-882.
Staiger, D., Apel, K., & Trepp, G., 1999. The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night. Plant Molecular Biology, 40(5), p 873-882.
D. Staiger, K. Apel, and G. Trepp, “The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night”, Plant Molecular Biology, vol. 40, 1999, pp. 873-882.
Staiger, D., Apel, K., Trepp, G.: The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night. Plant Molecular Biology. 40, 873-882 (1999).
Staiger, Dorothee, Apel, Klaus, and Trepp, Gian. “The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night”. Plant Molecular Biology 40.5 (1999): 873-882.

26 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7.
Meyer K, Köster T, Nolte C, Weinholdt C, Lewinski M, Grosse I, Staiger D., Genome Biol 18(1), 2017
PMID: 29084609
Transgenic Analysis Reveals 5' Abbreviated OsRGLP2 Promoter(s) as Responsive to Abiotic Stresses.
Shah SH, Noureen A, Deeba F, Sultana T, Dukowic-Schulze S, Chen C, Naqvi SMS., Mol Biotechnol 59(11-12), 2017
PMID: 28801881
Transcriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes in leaves and fruits.
Yuyama PM, Reis Júnior O, Ivamoto ST, Domingues DS, Carazzolle MF, Pereira GA, Charmetant P, Leroy T, Pereira LF., Mol Genet Genomics 291(1), 2016
PMID: 26334613
Do Cupins Have a Function Beyond Being Seed Storage Proteins?
Gábrišová D, Klubicová K, Danchenko M, Gömöry D, Berezhna VV, Skultety L, Miernyk JA, Rashydov N, Hajduch M., Front Plant Sci 6(), 2015
PMID: 26793203
Daytime soybean transcriptome fluctuations during water deficit stress.
Rodrigues FA, Fuganti-Pagliarini R, Marcolino-Gomes J, Nakayama TJ, Molinari HB, Lobo FP, Harmon FG, Nepomuceno AL., BMC Genomics 16(), 2015
PMID: 26149272
A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis.
Löhr B, Streitner C, Steffen A, Lange T, Staiger D., Mol Biol Rep 41(1), 2014
PMID: 24281950
Localization of tobacco germin-like protein 1 in leaf intercellular space.
Kishi-Kaboshi M, Muto H, Takeda A, Murata T, Hasebe M, Watanabe Y., Plant Physiol Biochem 85(), 2014
PMID: 25394794
An hnRNP-like RNA-binding protein affects alternative splicing by in vivo interaction with transcripts in Arabidopsis thaliana.
Streitner C, Köster T, Simpson CG, Shaw P, Danisman S, Brown JW, Staiger D., Nucleic Acids Res 40(22), 2012
PMID: 23042250
Molecular cloning and characterization of novel Morus alba germin-like protein gene which encodes for a silkworm gut digestion-resistant antimicrobial protein.
Patnaik BB, Kim DH, Oh SH, Song YS, Chanh ND, Kim JS, Jung WJ, Saha AK, Bindroo BB, Han YS., PLoS One 7(12), 2012
PMID: 23284650
Regulation of two germin-like protein genes during plum fruit development.
El-Sharkawy I, Mila I, Bouzayen M, Jayasankar S., J Exp Bot 61(6), 2010
PMID: 20202999
Regulation of output from the plant circadian clock.
Yakir E, Hilman D, Harir Y, Green RM., FEBS J 274(2), 2007
PMID: 17229141
Auto-regulation of the circadian slave oscillator component AtGRP7 and regulation of its targets is impaired by a single RNA recognition motif point mutation.
Schöning JC, Streitner C, Page DR, Hennig S, Uchida K, Wolf E, Furuya M, Staiger D., Plant J 52(6), 2007
PMID: 17924945
Diurnal regulation of plant growth.
Nozue K, Maloof JN., Plant Cell Environ 29(3), 2006
PMID: 17080594
Cloning of a pine germin-like protein (GLP) gene promoter and analysis of its activity in transgenic tobacco Bright Yellow 2 cells.
Mathieu M, Neutelings G, Hawkins S, Grenier E, David H., Physiol Plant 117(3), 2003
PMID: 12654044
The Arabidopsis circadian system.
McClung CR, Salomé PA, Michael TP., Arabidopsis Book 1(), 2002
PMID: 22303209
Phase-specific circadian clock regulatory elements in Arabidopsis.
Michael TP, McClung CR., Plant Physiol 130(2), 2002
PMID: 12376630
Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis.
Schaffer R, Landgraf J, Accerbi M, Simon V, Larson M, Wisman E., Plant Cell 13(1), 2001
PMID: 11158533
McClung CR., Annu Rev Plant Physiol Plant Mol Biol 52(), 2001
PMID: 11337395
RNA-binding proteins and circadian rhythms in Arabidopsis thaliana.
Staiger D., Philos Trans R Soc Lond B Biol Sci 356(1415), 2001
PMID: 11710982

46 References

Daten bereitgestellt von Europe PubMed Central.

A light-entrained circadian clock controls transcription of several plant genes.
Giuliano G, Hoffman NE, Ko K, Scolnik PA, Cashmore AR., EMBO J. 7(12), 1988
PMID: 3208743
Simplified high throughput protocol for northern hybridization.
Yang H, McLeese J, Weisbart M, Dionne JL, Lemaire I, Aubin RA., Nucleic Acids Res. 21(14), 1993
PMID: 8341618
The circadian system of Arabidopsis thaliana: forward and reverse genetic approaches.
Staiger D, Heintzen C., Chronobiol. Int. 16(1), 1999
PMID: 10023572
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
Jefferson RA, Burgess SM, Hirsh D., Proc. Natl. Acad. Sci. U.S.A. 83(22), 1986
PMID: 3534890
Significance of circadian gene expression in higher plants.
Beator J, Kloppstech K., Chronobiol. Int. 13(5), 1996
PMID: 8957584
Expression of the maize Cat3 catalase gene is under the influence of a circadian rhythm.
Redinbaugh MG, Sabre M, Scandalios JG., Proc. Natl. Acad. Sci. U.S.A. 87(17), 1990
PMID: 2395878
The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo.
Gallie DR, Sleat DE, Watts JW, Turner PC, Wilson TM., Nucleic Acids Res. 15(8), 1987
PMID: 3575095
A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.
Wang ZY, Kenigsbuch D, Sun L, Harel E, Ong MS, Tobin EM., Plant Cell 9(4), 1997
PMID: 9144958
Binary Agrobacterium vectors for plant transformation.
Bevan M., Nucleic Acids Res. 12(22), 1984
PMID: 6095209
AtGRP7, a nuclear RNA-binding protein as a component of a circadian-regulated negative feedback loop in Arabidopsis thaliana.
Heintzen C, Nater M, Apel K, Staiger D., Proc. Natl. Acad. Sci. U.S.A. 94(16), 1997
PMID: 9238008
Circadian Stomatal Rhythms in Epidermal Peels from Vicia faba.
Gorton HL, Williams WE, Binns ME, Gemmell CN, Leheny EA, Shepherd AC., Plant Physiol. 90(4), 1989
PMID: 16666931
DNA sequencing with chain-terminating inhibitors.
Sanger F, Nicklen S, Coulson AR., Proc. Natl. Acad. Sci. U.S.A. 74(12), 1977
PMID: 271968
Germin Gene Expression Is Induced in Wheat Leaves by Powdery Mildew Infection.
Hurkman WJ, Tanaka CK., Plant Physiol. 111(3), 1996
PMID: 12226325
Transcriptional regulation by a circadian rhythm.
Taylor WC., Plant Cell 1(2), 1989
PMID: 2535546
Characterization of a polyubiquitin gene from Arabidopsis thaliana.
Burke TJ, Callis J, Vierstra RD., Mol. Gen. Genet. 213(2-3), 1988
PMID: 2460733
Structure, expression and localization of a germin-like protein in barley (Hordeum vulgare L.) that is insolubilized in stressed leaves.
Vallelian-Bindschedler L, Mosinger E, Metraux JP, Schweizer P., Plant Mol. Biol. 37(2), 1998
PMID: 9617802
A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants.
Fejes E, Pay A, Kanevsky I, Szell M, Adam E, Kay S, Nagy F., Plant Mol. Biol. 15(6), 1990
PMID: 2103481
Molecular characterization of the oxalate oxidase involved in the response of barley to the powdery mildew fungus.
Zhou F, Zhang Z, Gregersen PL, Mikkelsen JD, de Neergaard E, Collinge DB, Thordal-Christensen H., Plant Physiol. 117(1), 1998
PMID: 9576772
A set of plant expression vectors for transcriptional and translational fusions.
Topfer R, Matzeit V, Gronenborn B, Schell J, Steinbiss HH., Nucleic Acids Res. 15(14), 1987
PMID: 3615207
Circadian and light-regulated expression of nitrate reductase in Arabidopsis.
Pilgrim ML, Caspar T, Quail PH, McClung CR., Plant Mol. Biol. 23(2), 1993
PMID: 8219070
Identification of tomato Lhc promoter regions necessary for circadian expression.
Piechulla B, Merforth N, Rudolph B., Plant Mol. Biol. 38(4), 1998
PMID: 9747810
Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.
Valvekens D, Van Montagu M, Van Lijsebettens M., Proc. Natl. Acad. Sci. U.S.A. 85(15), 1988
PMID: 16593964
Germin, a protein marker of early plant development, is an oxalate oxidase.
Lane BG, Dunwell JM, Ray JA, Schmitt MR, Cuming AC., J. Biol. Chem. 268(17), 1993
PMID: 8509360
cDNA sequence, genomic organization and differential expression of three Arabidopsis genes for germin/oxalate oxidase-like proteins.
Membre N, Berna A, Neutelings G, David A, David H, Staiger D, Saez Vasquez J, Raynal M, Delseny M, Bernier F., Plant Mol. Biol. 35(4), 1997
PMID: 9349269


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