REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS

JENA BP, GUMKOWSKI FD, KONIECZKO EM, Fischer von Mollard G, JAHN R, JAMIESON JD (1994)
JOURNAL OF CELL BIOLOGY 124(1): 43-53.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
JENA, BP; GUMKOWSKI, FD; KONIECZKO, EM; Fischer von Mollard, GabrieleUniBi; JAHN, R; JAMIESON, JD
Abstract / Bemerkung
Regulated secretion from pancreatic acinar cells occurs by exocytosis of zymogen granules (ZG) at the apical plasmalemma. ZGs originate from the TGN and undergo prolonged maturation and condensation. After exocytosis, the zymogen granule membrane (ZGM) is retrieved from the plasma membrane and ultimately reaches the TGN. In this study, we analyzed the fate of a low M(r) GTP-binding protein during induced exocytosis and membrane retrieval using immunoblots as well as light and electron microscopic immunocytochemistry. This 27-kD protein, identified by a monoclonal antibody that recognizes rab3A and B, may be a novel rab3 isoform. In resting acinar cells, the rab3-like protein was detected primarily on the cytoplasmic face of ZGs, with little labeling of the Golgi complex and no significant labeling of the apical plasmalemma or any other intracellular membranes. Stimulation of pancreatic lobules in vitro by carbamylcholine for 15 min, resulted in massive exocytosis that led to a near doubling of the area of the apical plasma membrane. However, no relocation of the rab3-like protein to the apical plasmalemma was seen. After 3 h of induced exocytosis, during which time approximately 90% of the ZGs is released, the rab3-like protein appeared to translocate to small vesicles and newly forming secretory granules in the TGN. No significant increase of the rab3-like protein was found in the cytosolic fraction at any time during stimulation. Since the protein is not detected on the apical plasmalemma after stimulation, we conclude that recycling may involve a membrane dissociation-association cycle that accompanies regulated exocytosis.
Erscheinungsjahr
1994
Zeitschriftentitel
JOURNAL OF CELL BIOLOGY
Band
124
Ausgabe
1
Seite(n)
43-53
ISSN
0021-9525
eISSN
1540-8140
Page URI
https://pub.uni-bielefeld.de/record/2374422

Zitieren

JENA BP, GUMKOWSKI FD, KONIECZKO EM, Fischer von Mollard G, JAHN R, JAMIESON JD. REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY. 1994;124(1):43-53.
JENA, B. P., GUMKOWSKI, F. D., KONIECZKO, E. M., Fischer von Mollard, G., JAHN, R., & JAMIESON, J. D. (1994). REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY, 124(1), 43-53. https://doi.org/10.1083/jcb.124.1.43
JENA, B. P., GUMKOWSKI, F. D., KONIECZKO, E. M., Fischer von Mollard, G., JAHN, R., and JAMIESON, J. D. (1994). REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY 124, 43-53.
JENA, B.P., et al., 1994. REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY, 124(1), p 43-53.
B.P. JENA, et al., “REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS”, JOURNAL OF CELL BIOLOGY, vol. 124, 1994, pp. 43-53.
JENA, B.P., GUMKOWSKI, F.D., KONIECZKO, E.M., Fischer von Mollard, G., JAHN, R., JAMIESON, J.D.: REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS. JOURNAL OF CELL BIOLOGY. 124, 43-53 (1994).
JENA, BP, GUMKOWSKI, FD, KONIECZKO, EM, Fischer von Mollard, Gabriele, JAHN, R, and JAMIESON, JD. “REDISTRIBUTION OF A RAB3-LIKE GTP-BINDING PROTEIN FROM SECRETORY GRANULES TO THE GOLGI-COMPLEX IN PANCREATIC ACINAR-CELLS DURING REGULATED EXOCYTOSIS”. JOURNAL OF CELL BIOLOGY 124.1 (1994): 43-53.

47 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.
Zhang X, Jiang S, Mitok KA, Li L, Attie AD, Martin TFJ., J Cell Biol 216(7), 2017
PMID: 28626000
Aquaporin-assisted and ER-mediated mitochondrial fission: a hypothesis.
Lee JS, Hou X, Bishop N, Wang S, Flack A, Cho WJ, Chen X, Mao G, Taatjes DJ, Sun F, Zhang K, Jena BP., Micron 47(), 2013
PMID: 23416165
Rab3D regulates amylase levels, not agonist-induced amylase release, in AR42J cells.
Limi S, Ojakian G, Raffaniello R., Cell Mol Biol Lett 17(2), 2012
PMID: 22367855
3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex.
Wang S, Lee JS, Bishop N, Jeremic A, Cho WJ, Chen X, Mao G, Taatjes DJ, Jena BP., Histochem Cell Biol 137(6), 2012
PMID: 22527693
Tctex-1, a novel interaction partner of Rab3D, is required for osteoclastic bone resorption.
Pavlos NJ, Cheng TS, Qin A, Ng PY, Feng HT, Ang ES, Carrello A, Sung CH, Jahn R, Zheng MH, Xu J., Mol Cell Biol 31(7), 2011
PMID: 21262767
Vesicle-associated membrane protein (VAMP) cleavage by a new metalloprotease from the Brazilian scorpion Tityus serrulatus.
Fletcher PL, Fletcher MD, Weninger K, Anderson TE, Martin BM., J Biol Chem 285(10), 2010
PMID: 20026600
ApRab3, a biosynthetic Rab protein, accumulates on the maturing phagosomes and symbiosomes in the tropical sea anemone, Aiptasia pulchella.
Hong MC, Huang YS, Lin WW, Fang LS, Chen MC., Comp Biochem Physiol B Biochem Mol Biol 152(3), 2009
PMID: 19110066
Small G proteins as key regulators of pancreatic digestive enzyme secretion.
Williams JA, Chen X, Sabbatini ME., Am J Physiol Endocrinol Metab 296(3), 2009
PMID: 19088252
Novel localization of Rab3D in rat intestinal goblet cells and Brunner's gland acinar cells suggests a role in early Golgi trafficking.
Valentijn JA, van Weeren L, Ultee A, Koster AJ., Am J Physiol Gastrointest Liver Physiol 293(1), 2007
PMID: 17395899
Rab3D redistribution and function in rat parotid acini.
Nguyen D, Jones A, Ojakian GK, Raffaniello RD., J Cell Physiol 197(3), 2003
PMID: 14566969
Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles.
Cho SJ, Sattar AK, Jeong EH, Satchi M, Cho JA, Dash S, Mayes MS, Stromer MH, Jena BP., Proc Natl Acad Sci U S A 99(7), 2002
PMID: 11917120
Bisperoxovanadium complex promotes dopamine exocytosis in PC12 cells.
Bieger S, Morinville A, Maysinger D., Neurochem Int 40(4), 2002
PMID: 11792460
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R., Mol Cell Biol 22(18), 2002
PMID: 12192047
Rab3a and SNARE proteins: potential regulators of melanosome movement.
Scott G, Zhao Q., J Invest Dermatol 116(2), 2001
PMID: 11180007
Supramaximal cholecystokinin displaces Munc18c from the pancreatic acinar basal surface, redirecting apical exocytosis to the basal membrane.
Gaisano HY, Lutz MP, Leser J, Sheu L, Lynch G, Tang L, Tamori Y, Trimble WS, Salapatek AM., J Clin Invest 108(11), 2001
PMID: 11733555
Actin coating of secretory granules during regulated exocytosis correlates with the release of rab3D.
Valentijn JA, Valentijn K, Pastore LM, Jamieson JD., Proc Natl Acad Sci U S A 97(3), 2000
PMID: 10655489
Mist1 expression is a common link among serous exocrine cells exhibiting regulated exocytosis.
Pin CL, Bonvissuto AC, Konieczny SF., Anat Rec 259(2), 2000
PMID: 10820318
Real-time studies of zymogen granule exocytosis in intact rat pancreatic acinar cells.
Campos-Toimil M, Edwardson JM, Thomas P., J Physiol 528 Pt 2(), 2000
PMID: 11034621
Expression and localization of rab escort protein isoforms in parotid acinar cells from rat.
Chan D, Lin J, Raffaniello RD., J Cell Physiol 185(3), 2000
PMID: 11056004
Expression and localization of Rab3D in rat parotid gland.
Raffaniello RD, Lin J, Schwimmer R, Ojakian GK., Biochim Biophys Acta 1450(3), 1999
PMID: 10395946
Secretory vesicle-specific antibodies in the confocal study of exo-endocytosis dynamics.
Slembrouck D, Partoens P, Annaert W, De Potter WP., Methods 18(4), 1999
PMID: 10491276
Role of actin in regulated exocytosis and compensatory membrane retrieval: insights from an old acquaintance.
Valentijn K, Valentijn JA, Jamieson JD., Biochem Biophys Res Commun 266(3), 1999
PMID: 10603303
Expression of Rab3D N135I inhibits regulated secretion of ACTH in AtT-20 cells.
Baldini G, Wang G, Weber M, Zweyer M, Bareggi R, Witkin JW, Martelli AM., J Cell Biol 140(2), 1998
PMID: 9442106
On the role of rab GTPases: what can be learned from the developing pancreas.
Valentijn JA, Jamieson JD., Biochem Biophys Res Commun 243(2), 1998
PMID: 9480809
Surface dynamics in living acinar cells imaged by atomic force microscopy: identification of plasma membrane structures involved in exocytosis.
Schneider SW, Sritharan KC, Geibel JP, Oberleithner H, Jena BP., Proc Natl Acad Sci U S A 94(1), 1997
PMID: 8990206
Establishment and immunocharacterization of an immortalized pancreatic cell line derived from the H-2Kb-tsA58 transgenic mouse.
Blouin R, Grondin G, Beaudoin J, Arita Y, Daigle N, Talbot BG, Lebel D, Morisset J., In Vitro Cell Dev Biol Anim 33(9), 1997
PMID: 9358288
Gi regulation of secretory vesicle swelling examined by atomic force microscopy.
Jena BP, Schneider SW, Geibel JP, Webster P, Oberleithner H, Sritharan KC., Proc Natl Acad Sci U S A 94(24), 1997
PMID: 9371843
Cloning of novel transcripts of the human guanine-nucleotide-exchange factor Mss4: in situ chromosomal mapping and expression in pancreatic cancer.
Müller-Pillasch F, Zimmerhackl F, Lacher U, Schultz N, Hameister H, Varga G, Friess H, Büchler M, Adler G, Gress TM., Genomics 46(3), 1997
PMID: 9441742
Possible involvement of Rab11 p24, a Ras-like small GTP-binding protein, in intracellular vesicular transport of isolated pancreatic acini.
Hori Y, Takeyama Y, Hiroyoshi M, Ueda T, Maeda A, Ohyanagi H, Saitoh Y, Kaibuchi K, Takai Y., Dig Dis Sci 41(1), 1996
PMID: 8565746
Effect of morphine on secretion of amylase from isolated parotid acini.
Miwa Y, Saeki M, Yamaji A, Maeda S, Saito K., Life Sci 59(21), 1996
PMID: 8937508
Rab proteins in gastric parietal cells: evidence for the membrane recycling hypothesis.
Calhoun BC, Goldenring JR., Yale J Biol Med 69(1), 1996
PMID: 9041684
Expression of Rab3D in dispersed chief cells from guinea pig stomach.
Raffaniello RD, Lin J, Wang F, Raufman JP., Biochim Biophys Acta 1311(2), 1996
PMID: 8630328
Co-operative regulation of endocytosis by three Rab5 isoforms.
Bucci C, Lütcke A, Steele-Mortimer O, Olkkonen VM, Dupree P, Chiariello M, Bruni CB, Simons K, Zerial M., FEBS Lett 366(1), 1995
PMID: 7789520
Molecular mechanisms in exocytosis.
Edwardson JM, Marciniak SJ., J Membr Biol 146(2), 1995
PMID: 7473682
Molecular mechanisms of bone resorption.
Teitelbaum SL, Abu-Amer Y, Ross FP., J Cell Biochem 59(1), 1995
PMID: 8530528
Rab proteins in regulated exocytosis.
Fischer von Mollard G, Stahl B, Li C, Südhof TC, Jahn R., Trends Biochem Sci 19(4), 1994
PMID: 8016866

32 References

Daten bereitgestellt von Europe PubMed Central.

rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles.
Fischer von Mollard G, Mignery GA, Baumert M, Perin MS, Hanson TJ, Burger PM, Jahn R, Sudhof TC., Proc. Natl. Acad. Sci. U.S.A. 87(5), 1990
PMID: 2155429
Sequential dissociation of the exocrine pancreas into lobules, acini, and individual cells.
Amsterdam A, Solomon TE, Jamieson JD., Methods Cell Biol. 20(), 1978
PMID: 692437
A common spectrum of polypeptides occurs in secretion granule membranes of different exocrine glands.
Cameron RS, Cameron PL, Castle JD., J. Cell Biol. 103(4), 1986
PMID: 3533952
Constitutive and regulated secretion of proteins.
Burgess TL, Kelly RB., Annu. Rev. Cell Biol. 3(), 1987
PMID: 3318877
Tissue distribution of smg p25A, a ras p21-like GTP-binding protein, studied by use of a specific monoclonal antibody.
Mizoguchi A, Kim S, Ueda T, Takai Y., Biochem. Biophys. Res. Commun. 162(3), 1989
PMID: 2504158
Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments.
Chavrier P, Parton RG, Hauri HP, Simons K, Zerial M., Cell 62(2), 1990
PMID: 2115402
Protein tyrosine phosphatase stimulates Ca(2+)-dependent amylase secretion from pancreatic acini.
Jena BP, Padfield PJ, Ingebritsen TS, Jamieson JD., J. Biol. Chem. 266(27), 1991
PMID: 1717440
Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic.
Walworth NC, Goud B, Kabcenell AK, Novick PJ., EMBO J. 8(6), 1989
PMID: 2504585
The cellular functions of small GTP-binding proteins.
Hall A., Science 249(4969), 1990
PMID: 2116664
Low molecular weight GTP-binding proteins associated with zymogen granule membranes from rat pancreas.
Padfield PJ, Jamieson JD., Biochem. Biophys. Res. Commun. 174(2), 1991
PMID: 1899570
GTP-binding proteins in intracellular transport.
Pfeffer SR., Trends Cell Biol. 2(2), 1992
PMID: 14731525
Membrane glycolipid trafficking in living, polarized pancreatic acinar cells: assessment by confocal microscopy.
Cornell-Bell AH, Otake LR, Sadler K, Thomas PG, Lawrence S, Olsen K, Gumkowski F, Peterson JR, Jamieson JD., Methods Cell Biol. 38(), 1993
PMID: 8246783
Small GTP-binding proteins in vesicular transport.
Balch WE., Trends Biochem. Sci. 15(12), 1990
PMID: 2077687
Regulation of vesicular traffic by a GTP-binding protein on the cytoplasmic surface of secretory vesicles in yeast.
Novick PJ, Goud B, Salminen A, Walworth NC, Nair J, Potenza M., Cold Spring Harb. Symp. Quant. Biol. 53 Pt 2(), 1988
PMID: 3151180
A small GTP-binding protein dissociates from synaptic vesicles during exocytosis.
Fischer von Mollard G, Sudhof TC, Jahn R., Nature 349(6304), 1991
PMID: 1845915
GE: a GTP-binding protein mediating exocytosis.
Gomperts BD., Annu. Rev. Physiol. 52(), 1990
PMID: 2109965
Condensation-sorting events in the rough endoplasmic reticulum of exocrine pancreatic cells.
Tooze J, Kern HF, Fuller SD, Howell KE., J. Cell Biol. 109(1), 1989
PMID: 2745555
Association of Rab3A with synaptic vesicles at late stages of the secretory pathway.
Matteoli M, Takei K, Cameron R, Hurlbut P, Johnston PA, Sudhof TC, Jahn R, De Camilli P., J. Cell Biol. 115(3), 1991
PMID: 1655810
Small GTP-binding proteins.
Takai Y, Kaibuchi K, Kikuchi A, Kawata M., Int. Rev. Cytol. 133(), 1992
PMID: 1577587
Cloning of a Rab3 isotype predominantly expressed in adipocytes.
Baldini G, Hohl T, Lin HY, Lodish HF., Proc. Natl. Acad. Sci. U.S.A. 89(11), 1992
PMID: 1594612
A synthetic peptide of the rab3a effector domain stimulates amylase release from permeabilized pancreatic acini.
Padfield PJ, Balch WE, Jamieson JD., Proc. Natl. Acad. Sci. U.S.A. 89(5), 1992
PMID: 1371881
Molecular cloning of YPT1/SEC4-related cDNAs from an epithelial cell line.
Chavrier P, Vingron M, Sander C, Simons K, Zerial M., Mol. Cell. Biol. 10(12), 1990
PMID: 2123294

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 8294505
PubMed | Europe PMC

Suchen in

Google Scholar