Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6
Dilcher M, Kohler B, Fischer von Mollard G (2001)
JOURNAL OF BIOLOGICAL CHEMISTRY 276(37): 34537-34544.
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Autor*in
Dilcher, M;
Kohler, B;
Fischer von Mollard, GabrieleUniBi
Einrichtung
Abstract / Bemerkung
SNARE proteins are required for fusion of transport vesicles with target membranes. Previously, we found that the yeast Q-SNARE Vti1p is involved in transport to the cis-Golgi, to the prevacuole/late endosome, and to the vacuole. Here we identified a previously uncharacterized gene, VTS1, and the R-SNARE YKT6 both as multicopy and as low copy suppressors of the growth and vacuolar transport defect in uti1-2 cells. Ykt6p was known to function in retrograde traffic to the cis-Golgi and homotypic vacuolar fusion. We found that VTI1 and YKT6 also interacted in traffic to the prevacuole and vacuole, indicating that these SNARE complexes contain Ykt6p, Vti1p, plus Pep12p and Ykt6p, Vti1p, Vam3p, plus Vam7p, respectively. As Ykt6p was required for several transport steps, R-SNAREs cannot be the sole determinants of specificity. To study the role of the 0 layer in the SNARE motif, we introduced the mutations vti1-Q158R and ykt6-R165Q. SNARE complexes to which Ykt6p contributed a fourth glutamine residue in the 0 layer were nonfunctional, suggesting an essential function for arginine in the 0 layer of these complexes. vti1-Q158R cells had severe defects in several transport steps, indicating that the second arginine in the 0 layer interfered with function.
Erscheinungsjahr
2001
Zeitschriftentitel
JOURNAL OF BIOLOGICAL CHEMISTRY
Band
276
Ausgabe
37
Seite(n)
34537-34544
ISSN
0021-9258
eISSN
1083-351X
Page URI
https://pub.uni-bielefeld.de/record/2374331
Zitieren
Dilcher M, Kohler B, Fischer von Mollard G. Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY. 2001;276(37):34537-34544.
Dilcher, M., Kohler, B., & Fischer von Mollard, G. (2001). Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY, 276(37), 34537-34544. https://doi.org/10.1074/jbc.M101551200
Dilcher, M, Kohler, B, and Fischer von Mollard, Gabriele. 2001. “Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6”. JOURNAL OF BIOLOGICAL CHEMISTRY 276 (37): 34537-34544.
Dilcher, M., Kohler, B., and Fischer von Mollard, G. (2001). Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY 276, 34537-34544.
Dilcher, M., Kohler, B., & Fischer von Mollard, G., 2001. Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY, 276(37), p 34537-34544.
M. Dilcher, B. Kohler, and G. Fischer von Mollard, “Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, 2001, pp. 34537-34544.
Dilcher, M., Kohler, B., Fischer von Mollard, G.: Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY. 276, 34537-34544 (2001).
Dilcher, M, Kohler, B, and Fischer von Mollard, Gabriele. “Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6”. JOURNAL OF BIOLOGICAL CHEMISTRY 276.37 (2001): 34537-34544.
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5 Einträge gefunden, die diesen Artikel zitieren
Synaptobrevin homolog YKT6 (UNIPROT: P36015)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Protein VTS1 (UNIPROT: Q08831)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Syntaxin PEP12 (UNIPROT: P32854)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Vacuolar morphogenesis protein 7 (UNIPROT: P32912)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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t-SNARE VTI1 (UNIPROT: Q04338)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Download in FASTA format
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