Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6

Dilcher M, Kohler B, Fischer von Mollard G (2001)
JOURNAL OF BIOLOGICAL CHEMISTRY 276(37): 34537-34544.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Dilcher, M; Kohler, B; Fischer von Mollard, GabrieleUniBi
Abstract / Bemerkung
SNARE proteins are required for fusion of transport vesicles with target membranes. Previously, we found that the yeast Q-SNARE Vti1p is involved in transport to the cis-Golgi, to the prevacuole/late endosome, and to the vacuole. Here we identified a previously uncharacterized gene, VTS1, and the R-SNARE YKT6 both as multicopy and as low copy suppressors of the growth and vacuolar transport defect in uti1-2 cells. Ykt6p was known to function in retrograde traffic to the cis-Golgi and homotypic vacuolar fusion. We found that VTI1 and YKT6 also interacted in traffic to the prevacuole and vacuole, indicating that these SNARE complexes contain Ykt6p, Vti1p, plus Pep12p and Ykt6p, Vti1p, Vam3p, plus Vam7p, respectively. As Ykt6p was required for several transport steps, R-SNAREs cannot be the sole determinants of specificity. To study the role of the 0 layer in the SNARE motif, we introduced the mutations vti1-Q158R and ykt6-R165Q. SNARE complexes to which Ykt6p contributed a fourth glutamine residue in the 0 layer were nonfunctional, suggesting an essential function for arginine in the 0 layer of these complexes. vti1-Q158R cells had severe defects in several transport steps, indicating that the second arginine in the 0 layer interfered with function.
Erscheinungsjahr
2001
Zeitschriftentitel
JOURNAL OF BIOLOGICAL CHEMISTRY
Band
276
Ausgabe
37
Seite(n)
34537-34544
ISSN
0021-9258
eISSN
1083-351X
Page URI
https://pub.uni-bielefeld.de/record/2374331

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Dilcher M, Kohler B, Fischer von Mollard G. Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY. 2001;276(37):34537-34544.
Dilcher, M., Kohler, B., & Fischer von Mollard, G. (2001). Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY, 276(37), 34537-34544. https://doi.org/10.1074/jbc.M101551200
Dilcher, M, Kohler, B, and Fischer von Mollard, Gabriele. 2001. “Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6”. JOURNAL OF BIOLOGICAL CHEMISTRY 276 (37): 34537-34544.
Dilcher, M., Kohler, B., and Fischer von Mollard, G. (2001). Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY 276, 34537-34544.
Dilcher, M., Kohler, B., & Fischer von Mollard, G., 2001. Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY, 276(37), p 34537-34544.
M. Dilcher, B. Kohler, and G. Fischer von Mollard, “Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, 2001, pp. 34537-34544.
Dilcher, M., Kohler, B., Fischer von Mollard, G.: Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY. 276, 34537-34544 (2001).
Dilcher, M, Kohler, B, and Fischer von Mollard, Gabriele. “Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6”. JOURNAL OF BIOLOGICAL CHEMISTRY 276.37 (2001): 34537-34544.

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Molecular mechanism to target the endosomal Mon1-Ccz1 GEF complex to the pre-autophagosomal structure.
Gao J, Langemeyer L, Kümmel D, Reggiori F, Ungermann C., Elife 7(), 2018
PMID: 29446751
Non-canonical role of the SNARE protein Ykt6 in autophagosome-lysosome fusion.
Takáts S, Glatz G, Szenci G, Boda A, Horváth GV, Hegedűs K, Kovács AL, Juhász G., PLoS Genet 14(4), 2018
PMID: 29694367
Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17.
Matsui T, Jiang P, Nakano S, Sakamaki Y, Yamamoto H, Mizushima N., J Cell Biol 217(8), 2018
PMID: 29789439
Cargo trafficking from the trans-Golgi network towards the endosome.
Kim K, Gadila SKG., Biol Cell 108(8), 2016
PMID: 27061938
The Ccz1-Mon1-Rab7 module and Rab5 control distinct steps of autophagy.
Hegedűs K, Takáts S, Boda A, Jipa A, Nagy P, Varga K, Kovács AL, Juhász G., Mol Biol Cell 27(20), 2016
PMID: 27559127
A voltage-gated calcium channel regulates lysosomal fusion with endosomes and autophagosomes and is required for neuronal homeostasis.
Tian X, Gala U, Zhang Y, Shang W, Nagarkar Jaiswal S, di Ronza A, Jaiswal M, Yamamoto S, Sandoval H, Duraine L, Sardiello M, Sillitoe RV, Venkatachalam K, Fan H, Bellen HJ, Tong C., PLoS Biol 13(3), 2015
PMID: 25811491
Functional homologies in vesicle tethering.
Kuhlee A, Raunser S, Ungermann C., FEBS Lett 589(19 pt a), 2015
PMID: 26072291
Endocytosis and autophagy: exploitation or cooperation?
Tooze SA, Abada A, Elazar Z., Cold Spring Harb Perspect Biol 6(5), 2014
PMID: 24789822
Route to destruction: autophagosomes SNARE lysosomes.
Krämer H., J Cell Biol 201(4), 2013
PMID: 23671308
Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in Drosophila.
Takáts S, Nagy P, Varga Á, Pircs K, Kárpáti M, Varga K, Kovács AL, Hegedűs K, Juhász G., J Cell Biol 201(4), 2013
PMID: 23671310
Autophagy at sea.
Martens S, Rusten TE, Kraft C., Autophagy 9(9), 2013
PMID: 23917436
[NSI+] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes.
Nizhnikov AA, Magomedova ZM, Rubel AA, Kondrashkina AM, Inge-Vechtomov SG, Galkin AP., Curr Genet 58(1), 2012
PMID: 22215010
Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex.
Lobingier BT, Merz AJ., Mol Biol Cell 23(23), 2012
PMID: 23051737
The Caenorhabditis elegans GARP complex contains the conserved Vps51 subunit and is required to maintain lysosomal morphology.
Luo L, Hannemann M, Koenig S, Hegermann J, Ailion M, Cho MK, Sasidharan N, Zweckstetter M, Rensing SA, Eimer S., Mol Biol Cell 22(14), 2011
PMID: 21613545
Involvement of Vts1, a structure-specific RNA-binding protein, in Okazaki fragment processing in yeast.
Lee CH, Shin YK, Phung TT, Bae JS, Kang YH, Nguyen TA, Kim JH, Kim DH, Kang MJ, Bae SH, Seo YS., Nucleic Acids Res 38(5), 2010
PMID: 20007605
Lipid-Induced conformational switch controls fusion activity of longin domain SNARE Ykt6.
Wen W, Yu J, Pan L, Wei Z, Weng J, Wang W, Ong YS, Tran TH, Hong W, Zhang M., Mol Cell 37(3), 2010
PMID: 20159557
Farnesylation of the SNARE protein Ykt6 increases its stability and helical folding.
Pylypenko O, Schönichen A, Ludwig D, Ungermann C, Goody RS, Rak A, Geyer M., J Mol Biol 377(5), 2008
PMID: 18329045
Depalmitoylation of Ykt6 prevents its entry into the multivesicular body pathway.
Meiringer CT, Auffarth K, Hou H, Ungermann C., Traffic 9(9), 2008
PMID: 18541004
The NMR and X-ray structures of the Saccharomyces cerevisiae Vts1 SAM domain define a surface for the recognition of RNA hairpins.
Aviv T, Amborski AN, Zhao XS, Kwan JJ, Johnson PE, Sicheri F, Donaldson LW., J Mol Biol 356(2), 2006
PMID: 16375924
Sequence-specific recognition of RNA hairpins by the SAM domain of Vts1p.
Aviv T, Lin Z, Ben-Ari G, Smibert CA, Sicheri F., Nat Struct Mol Biol 13(2), 2006
PMID: 16429151
RNA recognition by the Vts1p SAM domain.
Johnson PE, Donaldson LW., Nat Struct Mol Biol 13(2), 2006
PMID: 16429155
Shape-specific recognition in the structure of the Vts1p SAM domain with RNA.
Oberstrass FC, Lee A, Stefl R, Janis M, Chanfreau G, Allain FH., Nat Struct Mol Biol 13(2), 2006
PMID: 16429156
Genetic and functional interaction between Ryh1 and Ypt3: two Rab GTPases that function in S. pombe secretory pathway.
He Y, Sugiura R, Ma Y, Kita A, Deng L, Takegawa K, Matsuoka K, Shuntoh H, Kuno T., Genes Cells 11(3), 2006
PMID: 16483310
Extracting sequence motifs and the phylogenetic features of SNARE-dependent membrane traffic.
Yoshizawa AC, Kawashima S, Okuda S, Fujita M, Itoh M, Moriya Y, Hattori M, Kanehisa M., Traffic 7(8), 2006
PMID: 16882042
Probing protein palmitoylation at the yeast vacuole.
Meiringer CT, Ungermann C., Methods 40(2), 2006
PMID: 17012029
Protein transport from the late Golgi to the vacuole in the yeast Saccharomyces cerevisiae.
Bowers K, Stevens TH., Biochim Biophys Acta 1744(3), 2005
PMID: 15913810
The SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion.
Dietrich LE, Gurezka R, Veit M, Ungermann C., EMBO J 23(1), 2004
PMID: 14685280
Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation.
Fukasawa M, Varlamov O, Eng WS, Söllner TH, Rothman JE., Proc Natl Acad Sci U S A 101(14), 2004
PMID: 15044687
Mammalian ykt6 is a neuronal SNARE targeted to a specialized compartment by its profilin-like amino terminal domain.
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PMID: 12589064
Membrane fusion.
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PMID: 12600315
The RNA-binding SAM domain of Smaug defines a new family of post-transcriptional regulators.
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PMID: 12858164
Current awareness on yeast.
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PMID: 11816036
Membrane fusion.
Jahn R, Grubmüller H., Curr Opin Cell Biol 14(4), 2002
PMID: 12383801

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