The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners

Kreykenbohm V, Wenzel D, Antonin W, Atlachkine V, Fischer von Mollard G (2002)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Kreykenbohm, V; Wenzel, D; Antonin, W; Atlachkine, V; Fischer von Mollard, GabrieleUniBi
Abstract / Bemerkung
Two mammalian proteins, vti1a and vti1b, are homologous to the yeast Q-SNARE Vti1p which is part of several SNARE complexes in different transport steps. In vitro experiments suggest distinct functions for vti1a and vti1b. Here we compared the subcellular localization of endogenous vti1a and vti1b by immunofluorescence and immuno-electron microscopy. Both proteins had a distinct but overlapping localization. vti1a was found predominantly on the Golgi and the TGN, vti1b mostly on tubules and vesicles in the TGN area and on endosomes. vti1a coimmunoprecipitated with VAMP-4, syntaxin 6, and syntaxin 16. These four SNAREs could assemble into a SNARE complex of conserved structure because one SNARE motif of each subgroup is present. vtila-beta, VAMP-4, syntaxin 6, and syntaxin 16 are coenriched with small synaptic vesicles and with clathrin-coated vesicles isolated from rat brain synaptosomes. Therefore, this SNARE complex may have a role in synaptic vesicle biogenesis or recycling.
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Kreykenbohm V, Wenzel D, Antonin W, Atlachkine V, Fischer von Mollard G. The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. EUROPEAN JOURNAL OF CELL BIOLOGY. 2002;81(5):273-280.
Kreykenbohm, V., Wenzel, D., Antonin, W., Atlachkine, V., & Fischer von Mollard, G. (2002). The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. EUROPEAN JOURNAL OF CELL BIOLOGY, 81(5), 273-280.
Kreykenbohm, V., Wenzel, D., Antonin, W., Atlachkine, V., and Fischer von Mollard, G. (2002). The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. EUROPEAN JOURNAL OF CELL BIOLOGY 81, 273-280.
Kreykenbohm, V., et al., 2002. The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. EUROPEAN JOURNAL OF CELL BIOLOGY, 81(5), p 273-280.
V. Kreykenbohm, et al., “The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners”, EUROPEAN JOURNAL OF CELL BIOLOGY, vol. 81, 2002, pp. 273-280.
Kreykenbohm, V., Wenzel, D., Antonin, W., Atlachkine, V., Fischer von Mollard, G.: The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. EUROPEAN JOURNAL OF CELL BIOLOGY. 81, 273-280 (2002).
Kreykenbohm, V, Wenzel, D, Antonin, W, Atlachkine, V, and Fischer von Mollard, Gabriele. “The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners”. EUROPEAN JOURNAL OF CELL BIOLOGY 81.5 (2002): 273-280.

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Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.
Rorsman P, Ashcroft FM., Physiol Rev 98(1), 2018
PMID: 29212789
Presynaptic origins of distinct modes of neurotransmitter release.
Chanaday NL, Kavalali ET., Curr Opin Neurobiol 51(), 2018
PMID: 29597140
Endosomal and Phagosomal SNAREs.
Dingjan I, Linders PTA, Verboogen DRJ, Revelo NH, Ter Beest M, van den Bogaart G., Physiol Rev 98(3), 2018
PMID: 29790818
The enigmatic endosome - sorting the ins and outs of endocytic trafficking.
Naslavsky N, Caplan S., J Cell Sci 131(13), 2018
PMID: 29980602
Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.
Emperador-Melero J, Huson V, van Weering J, Bollmann C, Fischer von Mollard G, Toonen RF, Verhage M., Nat Commun 9(1), 2018
PMID: 30143604
Selective molecular impairment of spontaneous neurotransmission modulates synaptic efficacy.
Crawford DC, Ramirez DM, Trauterman B, Monteggia LM, Kavalali ET., Nat Commun 8(), 2017
PMID: 28186166
A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes.
Tie HC, Mahajan D, Chen B, Cheng L, VanDongen AM, Lu L., Mol Biol Cell 27(5), 2016
PMID: 26764092
Platelet secretion: From haemostasis to wound healing and beyond.
Golebiewska EM, Poole AW., Blood Rev 29(3), 2015
PMID: 25468720
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW., J Biol Chem 290(3), 2015
PMID: 25404741
EARP is a multisubunit tethering complex involved in endocytic recycling.
Schindler C, Chen Y, Pu J, Guo X, Bonifacino JS., Nat Cell Biol 17(5), 2015
PMID: 25799061
Molecular underpinnings of synaptic vesicle pool heterogeneity.
Crawford DC, Kavalali ET., Traffic 16(4), 2015
PMID: 25620674
Single nucleotide polymorphisms in VTI1A gene contribute to the susceptibility of Chinese population to non-small cell lung cancer.
Su WM, Chen ZH, Zhang XC, Su J, Xie Z, Yan HH, Yang JJ, Chen HJ, Zhou Q, Zhong WZ, Guo WB, Chen SL, Wu YL., Int J Biol Markers 30(3), 2015
PMID: 25744365
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, Sørensen JB., EMBO J 33(15), 2014
PMID: 24902738
The eukaryotic signal sequence, YGRL, targets the chlamydial inclusion.
Kabeiseman EJ, Cichos KH, Moore ER., Front Cell Infect Microbiol 4(), 2014
PMID: 25309881
The small molecule dispergo tubulates the endoplasmic reticulum and inhibits export.
Lu L, Hannoush RN, Goess BC, Varadarajan S, Shair MD, Kirchhausen T., Mol Biol Cell 24(7), 2013
PMID: 23389632
Molecular characterization and functional significance of the Vti family of SNARE proteins in tick salivary glands.
Villarreal AM, Adamson SW, Browning RE, Budachetri K, Sajid MS, Karim S., Insect Biochem Mol Biol 43(5), 2013
PMID: 23499931
New breast cancer risk variant discovered at 10q25 in East Asian women.
Shi J, Sung H, Zhang B, Lu W, Choi JY, Xiang YB, Kim MK, Iwasaki M, Long J, Ji BT, Park SK, Zheng Y, Tsugane S, Yoo KY, Wang W, Noh DY, Han W, Kim SW, Lee MH, Lee JW, Lee JY, Shen CY, Matsuo K, Ahn SH, Gao YT, Shu XO, Cai Q, Kang D, Zheng W., Cancer Epidemiol Biomarkers Prev 22(7), 2013
PMID: 23677579
Vesicle-associated membrane protein 4 and syntaxin 6 interactions at the chlamydial inclusion.
Kabeiseman EJ, Cichos K, Hackstadt T, Lucas A, Moore ER., Infect Immun 81(9), 2013
PMID: 23798538
Vti1a identifies a vesicle pool that preferentially recycles at rest and maintains spontaneous neurotransmission.
Ramirez DM, Khvotchev M, Trauterman B, Kavalali ET., Neuron 73(1), 2012
PMID: 22243751
The role of non-canonical SNAREs in synaptic vesicle recycling.
Ramirez DM, Kavalali ET., Cell Logist 2(1), 2012
PMID: 22645707
Regulation of integrin endocytic recycling and chemotactic cell migration by syntaxin 6 and VAMP3 interaction.
Riggs KA, Hasan N, Humphrey D, Raleigh C, Nevitt C, Corbin D, Hu C., J Cell Sci 125(pt 16), 2012
PMID: 22573826
Lack of the endosomal SNAREs vti1a and vti1b led to significant impairments in neuronal development.
Kunwar AJ, Rickmann M, Backofen B, Browski SM, Rosenbusch J, Schöning S, Fleischmann T, Krieglstein K, Fischer von Mollard G., Proc Natl Acad Sci U S A 108(6), 2011
PMID: 21262811
Proteomic investigations of the synaptic vesicle interactome.
Barth J, Volknandt W., Expert Rev Proteomics 8(2), 2011
PMID: 21501014
Epsin N-terminal homology domains bind on opposite sides of two SNAREs.
Wang J, Gossing M, Fang P, Zimmermann J, Li X, von Mollard GF, Niu L, Teng M., Proc Natl Acad Sci U S A 108(30), 2011
PMID: 21746902
Genomic sequencing of colorectal adenocarcinomas identifies a recurrent VTI1A-TCF7L2 fusion.
Bass AJ, Lawrence MS, Brace LE, Ramos AH, Drier Y, Cibulskis K, Sougnez C, Voet D, Saksena G, Sivachenko A, Jing R, Parkin M, Pugh T, Verhaak RG, Stransky N, Boutin AT, Barretina J, Solit DB, Vakiani E, Shao W, Mishina Y, Warmuth M, Jimenez J, Chiang DY, Signoretti S, Kaelin WG, Spardy N, Hahn WC, Hoshida Y, Ogino S, DePinho RA, Chin L, Garraway LA, Fuchs CS, Baselga J, Tabernero J, Gabriel S, Lander ES, Getz G, Meyerson M., Nat Genet 43(10), 2011
PMID: 21892161
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann U, Schäfer C, Riedel D, Jahn R, Rizzoli SO., Microsc Res Tech 73(6), 2010
PMID: 19937745
Neuronal Ca2+ sensor VILIP-1 leads to the upregulation of functional alpha4beta2 nicotinic acetylcholine receptors in hippocampal neurons.
Zhao CJ, Noack C, Brackmann M, Gloveli T, Maelicke A, Heinemann U, Anand R, Braunewell KH., Mol Cell Neurosci 40(2), 2009
PMID: 19063970
Differential effects of depletion of ARL1 and ARFRP1 on membrane trafficking between the trans-Golgi network and endosomes.
Nishimoto-Morita K, Shin HW, Mitsuhashi H, Kitamura M, Zhang Q, Johannes L, Nakayama K., J Biol Chem 284(16), 2009
PMID: 19224922
Endosomal SNARE proteins regulate CFTR activity and trafficking in epithelial cells.
Bilan F, Nacfer M, Fresquet F, Norez C, Melin P, Martin-Berge A, Costa de Beauregard MA, Becq F, Kitzis A, Thoreau V., Exp Cell Res 314(11-12), 2008
PMID: 18570918
Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin.
Kanwar N, Fayyazi A, Backofen B, Nitsche M, Dressel R, von Mollard GF., Cell Tissue Res 334(2), 2008
PMID: 18923845
Dynamics of endogenous ATP7A (Menkes protein) in intestinal epithelial cells: copper-dependent redistribution between two intracellular sites.
Nyasae L, Bustos R, Braiterman L, Eipper B, Hubbard A., Am J Physiol Gastrointest Liver Physiol 292(4), 2007
PMID: 17158254
The PEROXIN11 protein family controls peroxisome proliferation in Arabidopsis.
Orth T, Reumann S, Zhang X, Fan J, Wenzel D, Quan S, Hu J., Plant Cell 19(1), 2007
PMID: 17220199
Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis.
Sanmartín M, Ordóñez A, Sohn EJ, Robert S, Sánchez-Serrano JJ, Surpin MA, Raikhel NV, Rojo E., Proc Natl Acad Sci U S A 104(9), 2007
PMID: 17360696
G1 phase-dependent nucleolar accumulation of human histone H1x.
Stoldt S, Wenzel D, Schulze E, Doenecke D, Happel N., Biol Cell 99(10), 2007
PMID: 17868027
Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity.
Brandhorst D, Zwilling D, Rizzoli SO, Lippert U, Lang T, Jahn R., Proc Natl Acad Sci U S A 103(8), 2006
PMID: 16469845
Molecular basis of pregnancy-induced breast cancer protection.
Russo J, Balogh GA, Heulings R, Mailo DA, Moral R, Russo PA, Sheriff F, Vanegas J, Russo IH., Eur J Cancer Prev 15(4), 2006
PMID: 16835503
Functionally different pools of Shiga toxin receptor, globotriaosyl ceramide, in HeLa cells.
Falguières T, Römer W, Amessou M, Afonso C, Wolf C, Tabet JC, Lamaze C, Johannes L., FEBS J 273(22), 2006
PMID: 17059464
The cargo in vacuolar storage protein transport vesicles is stratified.
Wenzel D, Schauermann G, von Lüpke A, Hinz G., Traffic 6(1), 2005
PMID: 15569244
Suicide attempt and basic mechanisms in neural conduction: relationships to the SCN8A and VAMP4 genes.
Wasserman D, Geijer T, Rozanov V, Wasserman J., Am J Med Genet B Neuropsychiatr Genet 133B(1), 2005
PMID: 15635639
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Nakamura N, Fukuda H, Kato A, Hirose S., Mol Biol Cell 16(4), 2005
PMID: 15689499
Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis.
Niihama M, Uemura T, Saito C, Nakano A, Sato MH, Tasaka M, Morita MT., Curr Biol 15(6), 2005
PMID: 15797025
Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct.
Barile M, Pisitkun T, Yu MJ, Chou CL, Verbalis MJ, Shen RF, Knepper MA., Mol Cell Proteomics 4(8), 2005
PMID: 15905145
Trans-Golgi network syntaxin 10 functions distinctly from syntaxins 6 and 16.
Wang Y, Tai G, Lu L, Johannes L, Hong W, Tang BL., Mol Membr Biol 22(4), 2005
PMID: 16154903
Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells.
Kuliawat R, Kalinina E, Bock J, Fricker L, McGraw TE, Kim SR, Zhong J, Scheller R, Arvan P., Mol Biol Cell 15(4), 2004
PMID: 14742717
Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling.
Blondeau F, Ritter B, Allaire PD, Wasiak S, Girard M, Hussain NK, Angers A, Legendre-Guillemin V, Roy L, Boismenu D, Kearney RE, Bell AW, Bergeron JJ, McPherson PS., Proc Natl Acad Sci U S A 101(11), 2004
PMID: 15007177
GLUT4 recycles via a trans-Golgi network (TGN) subdomain enriched in Syntaxins 6 and 16 but not TGN38: involvement of an acidic targeting motif.
Shewan AM, van Dam EM, Martin S, Luen TB, Hong W, Bryant NJ, James DE., Mol Biol Cell 14(3), 2003
PMID: 12631717
Biochemical sub-fractionation of the mammalian Golgi apparatus.
Taguchi T, Pypaert M, Warren G., Traffic 4(5), 2003
PMID: 12713662
Deletion of the SNARE vti1b in mice results in the loss of a single SNARE partner, syntaxin 8.
Atlashkin V, Kreykenbohm V, Eskelinen EL, Wenzel D, Fayyazi A, Fischer von Mollard G., Mol Cell Biol 23(15), 2003
PMID: 12861006
Characterization of the in vitro retrograde transport of MPR46.
Medigeshi GR, Schu P., Traffic 4(11), 2003
PMID: 14617361


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