Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells

Chan JW, Taylor DS, Zwerdling T, Lane SM, Ihara K, Huser T (2006)
Biophysical Journal 90(2): 648-656.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Chan, J. W.; Taylor, D. S.; Zwerdling, T.; Lane, S. M.; Ihara, K.; Huser, ThomasUniBi
Abstract / Bemerkung
Current methods for identifying neoplastic cells and discerning them from their normal counterparts are often nonspecific, slow, biologically perturbing, or a combination thereof. Here, we show that single-cell micro-Raman spectroscopy averts these shortcomings and can be used to discriminate between unfixed normal human lymphocytes and transformed Jurkat and Raji lymphocyte cell lines based on their biomolecular Raman signatures. We demonstrate that single-cell Raman spectra provide a highly reproducible biomolecular fingerprint of each cell type. Characteristic peaks, mostly due to different DNA and protein concentrations, allow for discerning normal lymphocytes from transformed lymphocytes with high confidence (p << 0.05). Spectra are also compared and analyzed by principal component analysis to demonstrate that normal and transformed cells form distinct clusters that can be defined using just two principal components. The method is shown to have a sensitivity of 98.3% for cancer detection, with 97.2% of the cells being correctly classified as belonging to the normal or transformed type. These results demonstrate the potential application of confocal micro-Raman spectroscopy as a clinical tool for single cancer cell detection based on intrinsic biomolecular signatures, therefore eliminating the need for exogenous fluorescent labeling.
Biophysical Journal
Page URI


Chan JW, Taylor DS, Zwerdling T, Lane SM, Ihara K, Huser T. Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. Biophysical Journal. 2006;90(2):648-656.
Chan, J. W., Taylor, D. S., Zwerdling, T., Lane, S. M., Ihara, K., & Huser, T. (2006). Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. Biophysical Journal, 90(2), 648-656. https://doi.org/10.1529/biophysj.105.066761
Chan, J. W., Taylor, D. S., Zwerdling, T., Lane, S. M., Ihara, K., and Huser, Thomas. 2006. “Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells”. Biophysical Journal 90 (2): 648-656.
Chan, J. W., Taylor, D. S., Zwerdling, T., Lane, S. M., Ihara, K., and Huser, T. (2006). Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. Biophysical Journal 90, 648-656.
Chan, J.W., et al., 2006. Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. Biophysical Journal, 90(2), p 648-656.
J.W. Chan, et al., “Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells”, Biophysical Journal, vol. 90, 2006, pp. 648-656.
Chan, J.W., Taylor, D.S., Zwerdling, T., Lane, S.M., Ihara, K., Huser, T.: Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. Biophysical Journal. 90, 648-656 (2006).
Chan, J. W., Taylor, D. S., Zwerdling, T., Lane, S. M., Ihara, K., and Huser, Thomas. “Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells”. Biophysical Journal 90.2 (2006): 648-656.

181 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Detection of hydroquinone by Raman spectroscopy in patients with melasma before and after treatment.
Cabrera-Alonso R, Guevara E, Ramírez-Elías MG, Moncada B, González FJ., Skin Res Technol 25(1), 2019
PMID: 29790609
Diagnosis in a Preclinical Model of Bladder Pain Syndrome Using a Au/ZnO Nanorod-based SERS Substrate.
Lee S, Namgoong JM, Yu HY, Jue M, Kim G, Jeon S, Shin DM, Choo MS, Joo J, Pack CG, Kim JK., Nanomaterials (Basel) 9(2), 2019
PMID: 30736472
Waveguiding and SERS Simplified Raman Spectroscopy on Biological Samples.
Valpapuram I, Candeloro P, Coluccio ML, Parrotta EI, Giugni A, Das G, Cuda G, Di Fabrizio E, Perozziello G., Biosensors (Basel) 9(1), 2019
PMID: 30832416
Optimization of ZnO Nanorod-Based Surface Enhanced Raman Scattering Substrates for Bio-Applications.
Jue M, Lee S, Paulson B, Namgoong JM, Yu HY, Kim G, Jeon S, Shin DM, Choo MS, Joo J, Moon Y, Pack CG, Kim JK., Nanomaterials (Basel) 9(3), 2019
PMID: 30884889
Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging.
Daum R, Brauchle EM, Berrio DAC, Jurkowski TP, Schenke-Layland K., Sci Rep 9(1), 2019
PMID: 31065074
Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers.
Duś-Szachniewicz K, Drobczyński S, Woźniak M, Zduniak K, Ostasiewicz K, Ziółkowski P, Korzeniewska AK, Agrawal AK, Kołodziej P, Walaszek K, Bystydzieński Z, Rymkiewicz G., Sci Rep 9(1), 2019
PMID: 31285461
A droplet-based microfluidic chip as a platform for leukemia cell lysate identification using surface-enhanced Raman scattering.
Hassoun M, Rüger J, Kirchberger-Tolstik T, Schie IW, Henkel T, Weber K, Cialla-May D, Krafft C, Popp J., Anal Bioanal Chem 410(3), 2018
PMID: 28905087
Structural features of α-synuclein amyloid fibrils revealed by Raman spectroscopy.
Flynn JD, McGlinchey RP, Walker RL, Lee JC., J Biol Chem 293(3), 2018
PMID: 29191831
Raman detection and identification of normal and leukemic hematopoietic cells.
Managò S, Mirabelli P, Napolitano M, Zito G, De Luca AC., J Biophotonics 11(5), 2018
PMID: 29239116
Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies.
Shiramizu B, Oda R, Kamada N, Garcia MA, Shieh T, Maeda TA, Choi SY, Lim E, Misra A., J Biol Med Sci 2(1), 2018
PMID: 29962510
Laser stimulating ST36 with optical fiber induce blood component changes in mice: a Raman spectroscopy study.
Zhang H, Chen Z, Wu J, Chen N, Xu W, Li T, Liu S., J Biophotonics 11(6), 2018
PMID: 29446873
Discrimination of Single Living Rat Pancreatic α, β, δ, and Pancreatic Polypeptide (PP) Cells Using Raman Spectroscopy.
Rong X, Wei F, Luo RQ, Yuan X, Kuang QQ, Yin DP, Huang SS, Jiang YM, Liu H., Appl Spectrosc 72(5), 2018
PMID: 29350550
Label-free spectrochemical probe for determination of hemoglobin glycation in clinical blood samples.
Pandey R, Singh SP, Zhang C, Horowitz GL, Lue N, Galindo L, Dasari RR, Barman I., J Biophotonics 11(10), 2018
PMID: 29726123
Assessment of the radiotherapy effect for nasopharyngeal cancer using plasma surface-enhanced Raman spectroscopy technology.
Wu Q, Qiu S, Yu Y, Chen W, Lin H, Lin D, Feng S, Chen R., Biomed Opt Express 9(7), 2018
PMID: 29984106
Development of a Nanostructured Platform for Identifying HER2-Heterogeneity of Breast Cancer Cells by Surface-Enhanced Raman Scattering.
Téllez-Plancarte A, Haro-Poniatowski E, Picquart M, Morales-Méndez JG, Lara-Cruz C, Jiménez-Salazar JE, Damián-Matsumura P, Escobar-Alarcón L, Batina N., Nanomaterials (Basel) 8(7), 2018
PMID: 30036967
Resistance and Raman spectroscopy analysis of Parageobacillus thermantarcticus spores after γ-ray exposure.
Romano I, De Angelis A, Poli A, Ragni P, Lilla L, Zito G, Nicolaus B, De Luca AC, Di Donato P., Extremophiles 22(6), 2018
PMID: 30120597
Anti-cancer effect of bee venom on human MDA-MB-231 breast cancer cells using Raman spectroscopy.
Jung GB, Huh JE, Lee HJ, Kim D, Lee GJ, Park HK, Lee JD., Biomed Opt Express 9(11), 2018
PMID: 30460157
Broadband hyperspectral stimulated Raman scattering microscopy with a parabolic fiber amplifier source.
Figueroa B, Fu W, Nguyen T, Shin K, Manifold B, Wise F, Fu D., Biomed Opt Express 9(12), 2018
PMID: 31065417
Raman spectroscopic investigation on the molecular structure of apatite and collagen in osteoporotic cortical bone.
Pezzotti G, Rondinella A, Marin E, Zhu W, Aldini NN, Ulian G, Valdrè G., J Mech Behav Biomed Mater 65(), 2017
PMID: 27608424
Hyperspectral Raman imaging of human prostatic cells: An attempt to differentiate normal and malignant cell lines by univariate and multivariate data analysis.
Musto P, Calarco A, Pannico M, La Manna P, Margarucci S, Tafuri A, Peluso G., Spectrochim Acta A Mol Biomol Spectrosc 173(), 2017
PMID: 27718451
Effect of trehalose as an additive to dimethyl sulfoxide solutions on ice formation, cellular viability, and metabolism.
Solocinski J, Osgood Q, Wang M, Connolly A, Menze MA, Chakraborty N., Cryobiology 75(), 2017
PMID: 28063960
Raman Spectroscopy of Blood and Blood Components.
Atkins CG, Buckley K, Blades MW, Turner RFB., Appl Spectrosc 71(5), 2017
PMID: 28398071
Detection of early osteogenic commitment in primary cells using Raman spectroscopy.
Smith SJ, Emery R, Pitsillides A, Clarkin CE, Mahajan S., Analyst 142(11), 2017
PMID: 28503694
Raman spectroscopy and regenerative medicine: a review.
Ember KJI, Hoeve MA, McAughtrie SL, Bergholt MS, Dwyer BJ, Stevens MM, Faulds K, Forbes SJ, Campbell CJ., NPJ Regen Med 2(), 2017
PMID: 29302348
Raman spectroscopy differentiates between sensitive and resistant multiple myeloma cell lines.
Franco D, Trusso S, Fazio E, Allegra A, Musolino C, Speciale A, Cimino F, Saija A, Neri F, Nicolò MS, Guglielmino SPP., Spectrochim Acta A Mol Biomol Spectrosc 187(), 2017
PMID: 28645097
Leukemia cells detection based on electroporation assisted surface-enhanced Raman scattering.
Yu Y, Lin J, Lin D, Feng S, Chen W, Huang Z, Huang H, Chen R., Biomed Opt Express 8(9), 2017
PMID: 28966850
Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes.
Heraud P, Marzec KM, Zhang QH, Yuen WS, Carroll J, Wood BR., Sci Rep 7(1), 2017
PMID: 28827720
Raman endoscopy for monitoring the anticancer drug treatment of colorectal tumors in live mice.
Taketani A, Andriana BB, Matsuyoshi H, Sato H., Analyst 142(19), 2017
PMID: 28837186
Two sides of the same coin? Unraveling subtle differences between human embryonic and induced pluripotent stem cells by Raman spectroscopy.
Parrotta E, De Angelis MT, Scalise S, Candeloro P, Santamaria G, Paonessa M, Coluccio ML, Perozziello G, De Vitis S, Sgura A, Coluzzi E, Mollace V, Di Fabrizio EM, Cuda G., Stem Cell Res Ther 8(1), 2017
PMID: 29183402
Distinguishing Cancerous Liver Cells Using Surface-Enhanced Raman Spectroscopy.
Huang J, Liu S, Chen Z, Chen N, Pang F, Wang T., Technol Cancer Res Treat 15(1), 2016
PMID: 25432931
Raman spectroscopy as a diagnostic tool for monitoring acute nephritis.
Li J, Du Y, Qi J, Sneha R, Chang A, Mohan C, Shih WC., J Biophotonics 9(3), 2016
PMID: 25996441
Spectral pre and post processing for infrared and Raman spectroscopy of biological tissues and cells.
Byrne HJ, Knief P, Keating ME, Bonnier F., Chem Soc Rev 45(7), 2016
PMID: 26463830
A grid matrix-based Raman spectroscopic method to characterize different cell milieu in biopsied axillary sentinel lymph nodes of breast cancer patients.
Som D, Tak M, Setia M, Patil A, Sengupta A, Chilakapati CM, Srivastava A, Parmar V, Nair N, Sarin R, Badwe R., Lasers Med Sci 31(1), 2016
PMID: 26552923
Raman technologies in cancer diagnostics.
Austin LA, Osseiran S, Evans CL., Analyst 141(2), 2016
PMID: 26539569
Automated analysis of single cells using Laser Tweezers Raman Spectroscopy.
Casabella S, Scully P, Goddard N, Gardner P., Analyst 141(2), 2016
PMID: 26587766
Use of Raman spectroscopy to identify active spermatogenesis and Sertoli-cell-only tubules in mice.
Liu YF, Di L, Osterberg EC, He L, Li PS, Li Z., Andrologia 48(10), 2016
PMID: 26804545
Assessment of conjunctival microvilli abnormality by micro-Raman analysis - by G. Rusciano et al.
Rusciano G, Zito G, Pesce G, Del Prete S, Cennamo G, Sasso A., J Biophotonics 9(5), 2016
PMID: 26876569
Direct detection of malaria infected red blood cells by surface enhanced Raman spectroscopy.
Chen F, Flaherty BR, Cohen CE, Peterson DS, Zhao Y., Nanomedicine 12(6), 2016
PMID: 27015769
Raman spectroscopy: an evolving technique for live cell studies.
Smith R, Wright KL, Ashton L., Analyst 141(12), 2016
PMID: 27072718
A Stimulated Raman Scattering CMOS Pixel Using a High-Speed Charge Modulator and Lock-in Amplifier.
Lioe de X, Mars K, Kawahito S, Yasutomi K, Kagawa K, Yamada T, Hashimoto M., Sensors (Basel) 16(4), 2016
PMID: 27089339
A reliable Raman-spectroscopy-based approach for diagnosis, classification and follow-up of B-cell acute lymphoblastic leukemia.
Managò S, Valente C, Mirabelli P, Circolo D, Basile F, Corda D, De Luca AC., Sci Rep 6(), 2016
PMID: 27089853
Raman spectroscopy of endoscopic colonic biopsies from patients with ulcerative colitis to identify mucosal inflammation and healing.
Addis J, Mohammed N, Rotimi O, Magee D, Jha A, Subramanian V., Biomed Opt Express 7(5), 2016
PMID: 27231640
Label-free biomarkers of human embryonic stem cell differentiation to hepatocytes.
Tsikritsis D, Shi H, Wang Y, Velugotla S, Sršeň V, Elfick A, Downes A., Cytometry A 89(6), 2016
PMID: 27214589
Spectroscopic studies of anthracyclines: Structural characterization and in vitro tracking.
Szafraniec E, Majzner K, Farhane Z, Byrne HJ, Lukawska M, Oszczapowicz I, Chlopicki S, Baranska M., Spectrochim Acta A Mol Biomol Spectrosc 169(), 2016
PMID: 27372511
Molecular imaging with CARS micro-spectroscopy.
Cicerone M., Curr Opin Chem Biol 33(), 2016
PMID: 27400394
Brain tumor imaging of rat fresh tissue using terahertz spectroscopy.
Yamaguchi S, Fukushi Y, Kubota O, Itsuji T, Ouchi T, Yamamoto S., Sci Rep 6(), 2016
PMID: 27456312
Label-free optical sensor based on red blood cells laser tweezers Raman spectroscopy analysis for ABO blood typing.
Lin D, Zheng Z, Wang Q, Huang H, Huang Z, Yu Y, Qiu S, Wen C, Cheng M, Feng S., Opt Express 24(21), 2016
PMID: 27828195
Use of Raman spectroscopy to decrease time for identifying the species of Candida growth in cultures.
Chouthai NS, Shah AA, Salimnia H, Palyvoda O, Devpura S, Klein M, Asmar B., Avicenna J Med Biotechnol 7(1), 2015
PMID: 25926952
Raman spectrum reveals the cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis.
Zhang D, Feng Y, Zhang Q, Su X, Lu X, Liu S, Zhong L., Spectrochim Acta A Mol Biomol Spectrosc 141(), 2015
PMID: 25681805
Raman spectroscopy for medical diagnostics--From in-vitro biofluid assays to in-vivo cancer detection.
Kong K, Kendall C, Stone N, Notingher I., Adv Drug Deliv Rev 89(), 2015
PMID: 25809988
Analyses of ionizing radiation effects in vitro in peripheral blood lymphocytes with Raman spectroscopy.
Maguire A, Vegacarrascal I, White L, McClean B, Howe O, Lyng FM, Meade AD., Radiat Res 183(4), 2015
PMID: 25844945
Gold nanoaggregates for probing single-living cell based on surface-enhanced Raman spectroscopy.
Lu P, Wang J, Lin J, Lin J, Liu N, Huang Z, Li B, Zeng H, Chen R., J Biomed Opt 20(5), 2015
PMID: 25388888
3D plasmonic nanobowl platform for the study of exosomes in solution.
Lee C, Carney RP, Hazari S, Smith ZJ, Knudson A, Robertson CS, Lam KS, Wachsmann-Hogiu S., Nanoscale 7(20), 2015
PMID: 25939587
Classification and identification of pigmented cocci bacteria relevant to the soil environment via Raman spectroscopy.
Kumar V, Kampe B, Rösch P, Popp J., Environ Sci Pollut Res Int 22(24), 2015
PMID: 25940486
Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes.
James S, Fox J, Afsari F, Lee J, Clough S, Knight C, Ashmore J, Ashton P, Preham O, Hoogduijn M, Ponzoni Rde A, Hancock Y, Coles M, Genever P., Stem Cell Reports 4(6), 2015
PMID: 26070611
Modulated Raman Spectroscopy for Enhanced Cancer Diagnosis at the Cellular Level.
De Luca AC, Dholakia K, Mazilu M., Sensors (Basel) 15(6), 2015
PMID: 26110401
Diagnosis approach of chronic lymphocytic leukemia on unstained blood smears using Raman microspectroscopy and supervised classification.
Happillon T, Untereiner V, Beljebbar A, Gobinet C, Daliphard S, Cornillet-Lefebvre P, Quinquenel A, Delmer A, Troussard X, Klossa J, Manfait M., Analyst 140(13), 2015
PMID: 26017101
Raman spectroscopy for physiological investigations of tissues and cells.
Huser T, Chan J., Adv Drug Deliv Rev 89(), 2015
PMID: 26144996
Cellular discrimination using in vitro Raman micro spectroscopy: the role of the nucleolus.
Farhane Z, Bonnier F, Casey A, Maguire A, O'Neill L, Byrne HJ., Analyst 140(17), 2015
PMID: 26207998
Fluorescence modeling for optimized-binary compressive detection Raman spectroscopy.
Rehrauer OG, Mankani BR, Buzzard GT, Lucier BJ, Ben-Amotz D., Opt Express 23(18), 2015
PMID: 26368484
Detection of single amino acid mutation in human breast cancer by disordered plasmonic self-similar chain.
Coluccio ML, Gentile F, Das G, Nicastri A, Perri AM, Candeloro P, Perozziello G, Proietti Zaccaria R, Gongora JS, Alrasheed S, Fratalocchi A, Limongi T, Cuda G, Di Fabrizio E., Sci Adv 1(8), 2015
PMID: 26601267
Probing the interactions of CdTe quantum dots with pseudorabies virus.
Du T, Cai K, Han H, Fang L, Liang J, Xiao S., Sci Rep 5(), 2015
PMID: 26552937
Label-free blood plasma test based on surface-enhanced Raman scattering for tumor stages detection in nasopharyngeal cancer.
Lin D, Pan J, Huang H, Chen G, Qiu S, Shi H, Chen W, Yu Y, Feng S, Chen R., Sci Rep 4(), 2014
PMID: 24756153
The use of EEM fluorescence data and OPLS/UPLS-DA algorithm to discriminate between normal and cancer cell lines: a feasibility study.
Neves AC, Fernandes de Araújo R, Luiza Cabral de Sá Leitão Oliveira A, Antunes de Araújo A, de Lima KM., Analyst 139(10), 2014
PMID: 24695676
Highly effective hot spots for SERS signatures of live fibroblasts.
Radziuk D, Moehwald H., Nanoscale 6(11), 2014
PMID: 24788867
Investigating drug induced changes in single, living lymphocytes based on Raman micro-spectroscopy.
Schie IW, Alber L, Gryshuk AL, Chan JW., Analyst 139(11), 2014
PMID: 24756205
Raman spectroscopy as an analytical tool for melanoma research.
Brauchle E, Noor S, Holtorf E, Garbe C, Schenke-Layland K, Busch C., Clin Exp Dermatol 39(5), 2014
PMID: 24934918
Emerging technology: applications of Raman spectroscopy for prostate cancer.
Kast RE, Tucker SC, Killian K, Trexler M, Honn KV, Auner GW., Cancer Metastasis Rev 33(2-3), 2014
PMID: 24510129
Vibrational spectroscopic methods for cytology and cellular research.
Clemens G, Hands JR, Dorling KM, Baker MJ., Analyst 139(18), 2014
PMID: 25028699
Raman microspectroscopy for the early detection of pre-malignant changes in cervical tissue.
Rashid N, Nawaz H, Poon KW, Bonnier F, Bakhiet S, Martin C, O'Leary JJ, Byrne HJ, Lyng FM., Exp Mol Pathol 97(3), 2014
PMID: 25445502
Mechanical stress downregulates MHC class I expression on human cancer cell membrane.
La Rocca R, Tallerico R, Talib Hassan A, Das G, Lakshmikanth T, Matteucci M, Liberale C, Mesuraca M, Scumaci D, Gentile F, Cojoc G, Perozziello G, Ammendolia A, Gallo A, Kärre K, Cuda G, Candeloro P, Di Fabrizio E, Carbone E., PLoS One 9(12), 2014
PMID: 25541692
Raman spectral analysis of nasopharyngeal carcinoma cell line CNE2 after microwave radiation.
Ye Y, Chen Y, Su Y, Zou C, Huang Y, Ou L, Chen R., Biochem Cell Biol 91(2), 2013
PMID: 23527634
Raman microspectroscopy as a diagnostic tool to study single living nasopharyngeal carcinoma cell lines.
Li Z, Chen Y, Li Y, Chen W, Pan J, Su Y, Zou C., Biochem Cell Biol 91(3), 2013
PMID: 23668791
Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers.
Fu D, Holtom G, Freudiger C, Zhang X, Xie XS., J Phys Chem B 117(16), 2013
PMID: 23256635
Integrated microfluidic device for single-cell trapping and spectroscopy.
Liberale C, Cojoc G, Bragheri F, Minzioni P, Perozziello G, La Rocca R, Ferrara L, Rajamanickam V, Di Fabrizio E, Cristiani I., Sci Rep 3(), 2013
PMID: 23409249
Blood plasma surface-enhanced Raman spectroscopy for non-invasive optical detection of cervical cancer.
Feng S, Lin D, Lin J, Li B, Huang Z, Chen G, Zhang W, Wang L, Pan J, Chen R, Zeng H., Analyst 138(14), 2013
PMID: 23529624
A Raman-based endoscopic strategy for multiplexed molecular imaging.
Zavaleta CL, Garai E, Liu JT, Sensarn S, Mandella MJ, Van de Sompel D, Friedland S, Van Dam J, Contag CH, Gambhir SS., Proc Natl Acad Sci U S A 110(25), 2013
PMID: 23703909
Structural differences between native Hen egg white lysozyme and its fibrils under different environmental conditions.
Bhattacharya S, Ghosh S, Dasgupta S, Roy A., Spectrochim Acta A Mol Biomol Spectrosc 114(), 2013
PMID: 23786978
Reply: Raman microspectroscopy: a method with great promise but which also needs circumspection.
Ma M, Zhu Y, Yang S, Liu F, Li Z, He Z., Hum Reprod 28(9), 2013
PMID: 23787213
Raman endoscopy for the in situ investigation of advancing colorectal tumors in live model mice.
Taketani A, Hariyani R, Ishigaki M, Andriana BB, Sato H., Analyst 138(14), 2013
PMID: 23762892
Raman micro-spectroscopy can be used to investigate the developmental stage of the mouse oocyte.
Davidson B, Murray AA, Elfick A, Spears N., PLoS One 8(7), 2013
PMID: 23840882
Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation.
Pijanka JK, Stone N, Rutter AV, Forsyth N, Sockalingum GD, Yang Y, Sulé-Suso J., Analyst 138(17), 2013
PMID: 23841108
The role of asbestos morphology on their cellular toxicity: an in vitro 3D Raman/Rayleigh imaging study.
Yao S, Chen HH, Harte E, Ventura GD, Petibois C., Anal Bioanal Chem 405(27), 2013
PMID: 23846589
Raman microspectroscopy analysis in the treatment of acanthamoeba keratitis.
Rusciano G, Capriglione P, Pesce G, Del Prete S, Cennamo G, Di Cave D, Cerulli L, Sasso A., PLoS One 8(8), 2013
PMID: 23977228
Raman spectroscopic analysis of combat-related heterotopic ossification development.
Crane NJ, Polfer E, Elster EA, Potter BK, Forsberg JA., Bone 57(2), 2013
PMID: 24012700
Raman micro-spectroscopy tracing human lymphocyte activation.
Wesełucha-Birczyńska A, Kozicki M, Czepiel J, Birczyńska M., Analyst 138(23), 2013
PMID: 24145215
Quantitative reagent-free detection of fibrinogen levels in human blood plasma using Raman spectroscopy.
Poon KW, Lyng FM, Knief P, Howe O, Meade AD, Curtin JF, Byrne HJ, Vaughan J., Analyst 137(8), 2012
PMID: 22382127
Mechanochemistry of single red blood cells monitored using Raman tweezers.
Raj S, Marro M, Wojdyla M, Petrov D., Biomed Opt Express 3(4), 2012
PMID: 22574263
Raman imaging.
Stewart S, Priore RJ, Nelson MP, Treado PJ., Annu Rev Anal Chem (Palo Alto Calif) 5(), 2012
PMID: 22524218
Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
Bankapur A, Krishnamurthy RS, Zachariah E, Santhosh C, Chougule B, Praveen B, Valiathan M, Mathur D., PLoS One 7(4), 2012
PMID: 22514708
Preparation, characterization, and surface immobilization of native vesicles obtained by mechanical extrusion of mammalian cells.
Wu H, Oliver AE, Ngassam VN, Yee CK, Parikh AN, Yeh Y., Integr Biol (Camb) 4(6), 2012
PMID: 22543681
Differentiation of HaCaT cell and melanocyte from their malignant counterparts using micro-Raman spectroscopy guided by confocal imaging.
Wang H, Tsai TH, Zhao J, Lee AM, Lo BK, Yu M, Lui H, McLean DI, Zeng H., Photodermatol Photoimmunol Photomed 28(3), 2012
PMID: 22548397
Label-free serum ribonucleic acid analysis for colorectal cancer detection by surface-enhanced Raman spectroscopy and multivariate analysis.
Chen Y, Chen G, Feng S, Pan J, Zheng X, Su Y, Chen Y, Huang Z, Lin X, Lan F, Chen R, Zeng H., J Biomed Opt 17(6), 2012
PMID: 22734781
Confocal Raman microspectroscopy for skin characterization: a comparative study between human skin and pig skin.
Tfaili S, Gobinet C, Josse G, Angiboust JF, Manfait M, Piot O., Analyst 137(16), 2012
PMID: 22754919
Nanomaterials in complex biological systems: insights from Raman spectroscopy.
Drescher D, Kneipp J., Chem Soc Rev 41(17), 2012
PMID: 22782372
Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands.
Lin CY, Suhalim JL, Nien CL, Miljković MD, Diem M, Jester JV, Potma EO., J Biomed Opt 16(2), 2011
PMID: 21361667
In vivo lipidomics using single-cell Raman spectroscopy.
Wu H, Volponi JV, Oliver AE, Parikh AN, Simmons BA, Singh S., Proc Natl Acad Sci U S A 108(9), 2011
PMID: 21310969
Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples.
Canetta E, Mazilu M, De Luca AC, Carruthers AE, Dholakia K, Neilson S, Sargeant H, Briscoe T, Herrington CS, Riches AC., J Biomed Opt 16(3), 2011
PMID: 21456875
Raman imaging of single living cells: probing effects of non-cytotoxic doses of an anti-cancer drug.
Draux F, Gobinet C, Sulé-Suso J, Manfait M, Jeannesson P, Sockalingum GD., Analyst 136(13), 2011
PMID: 21562654
A biospectroscopic interrogation of fine needle aspirates points towards segregation between graded categories: an initial study towards diagnostic screening.
Kelly JG, Ahmadzai AA, Hermansen P, Pitt MA, Saidan Z, Martin-Hirsch PL, Martin FL., Anal Bioanal Chem 401(3), 2011
PMID: 21660416
Colorectal cancer detection by gold nanoparticle based surface-enhanced Raman spectroscopy of blood serum and statistical analysis.
Lin D, Feng S, Pan J, Chen Y, Lin J, Chen G, Xie S, Zeng H, Chen R., Opt Express 19(14), 2011
PMID: 21747512
Multivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy.
Smith ZJ, Strombom S, Wachsmann-Hogiu S., Opt Express 19(18), 2011
PMID: 21935055
Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies.
Candeloro P, Tirinato L, Malara N, Fregola A, Casals E, Puntes V, Perozziello G, Gentile F, Coluccio ML, Das G, Liberale C, De Angelis F, Di Fabrizio E., Analyst 136(21), 2011
PMID: 21879030
Full range characterization of the Raman spectra of organs in a murine model.
Huang N, Short M, Zhao J, Wang H, Lui H, Korbelik M, Zeng H., Opt Express 19(23), 2011
PMID: 22109167
Raman microspectroscopy detects epigenetic modifications in living Jurkat leukemic cells.
Poplineau M, Trussardi-Régnier A, Happillon T, Dufer J, Manfait M, Bernard P, Piot O, Antonicelli F., Epigenomics 3(6), 2011
PMID: 22126296
Monitoring human leukocyte antigen class I molecules by micro-Raman spectroscopy at single-cell level.
Das G, La Rocca R, Lakshmikanth T, Gentile F, Tallerico R, Zambetti LP, Devitt J, Candeloro P, De Angelis F, Carbone E, Di Fabrizio E., J Biomed Opt 15(2), 2010
PMID: 20459281
Raman tweezers spectroscopy of live, single red and white blood cells.
Bankapur A, Zachariah E, Chidangil S, Valiathan M, Mathur D., PLoS One 5(4), 2010
PMID: 20454686
Optimal algorithm for fluorescence suppression of modulated Raman spectroscopy.
Mazilu M, De Luca AC, Riches A, Herrington CS, Dholakia K., Opt Express 18(11), 2010
PMID: 20588999
Twenty-first century challenges for biomaterials.
Hench LL, Thompson I., J R Soc Interface 7 Suppl 4(), 2010
PMID: 20484227
Imaging with Raman spectroscopy.
Zhang Y, Hong H, Cai W., Curr Pharm Biotechnol 11(6), 2010
PMID: 20497112
Label-free cellular imaging by broadband coherent anti-Stokes Raman scattering microscopy.
Parekh SH, Lee YJ, Aamer KA, Cicerone MT., Biophys J 99(8), 2010
PMID: 20959111
Detection of doxorubicin-induced apoptosis of leukemic T-lymphocytes by laser tweezers Raman spectroscopy.
Moritz TJ, Taylor DS, Krol DM, Fritch J, Chan JW., Biomed Opt Express 1(4), 2010
PMID: 21258536
Towards detection and identification of circulating tumour cells using Raman spectroscopy.
Neugebauer U, Bocklitz T, Clement JH, Krafft C, Popp J., Analyst 135(12), 2010
PMID: 20941448
Dual-trap Raman tweezers for probing dynamics and heterogeneity of interacting microbial cells.
Li Y, Wang G, Yao HL, Liu J, Li YQ., J Biomed Opt 15(6), 2010
PMID: 21198212
Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation.
Dyson OF, Ford PW, Chen D, Li YQ, Akula SM., J Cell Mol Med 13(8b), 2009
PMID: 18752634
Raman spectroscopic differentiation of activated versus non-activated T lymphocytes: an in vitro study of an acute allograft rejection model.
Brown KL, Palyvoda OY, Thakur JS, Nehlsen-Cannarella SL, Fagoaga OR, Gruber SA, Auner GW., J Immunol Methods 340(1), 2009
PMID: 18996128
Raman tweezers and their application to the study of singly trapped eukaryotic cells.
Snook RD, Harvey TJ, Correia Faria E, Gardner P., Integr Biol (Camb) 1(1), 2009
PMID: 20023790
Chemical analysis in vivo and in vitro by Raman spectroscopy--from single cells to humans.
Wachsmann-Hogiu S, Weeks T, Huser T., Curr Opin Biotechnol 20(1), 2009
PMID: 19268566
Classification of fixed urological cells using Raman tweezers.
Harvey TJ, Hughes C, Ward AD, Faria EC, Henderson A, Clarke NW, Brown MD, Snook RD, Gardner P., J Biophotonics 2(1-2), 2009
PMID: 19343685
Raman and CARS microspectroscopy of cells and tissues.
Krafft C, Dietzek B, Popp J., Analyst 134(6), 2009
PMID: 19475129
Interaction of gold nanoparticles with mitochondria.
Karataş OF, Sezgin E, Aydin O, Culha M., Colloids Surf B Biointerfaces 71(2), 2009
PMID: 19329288
Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells.
Huser T, Orme CA, Hollars CW, Corzett MH, Balhorn R., J Biophotonics 2(5), 2009
PMID: 19373853
Surface-enhanced Raman scattering of rat tissues.
Aydin O, Kahraman M, Kiliç E, Culha M., Appl Spectrosc 63(6), 2009
PMID: 19531293
Rapid delivery of silver nanoparticles into living cells by electroporation for surface-enhanced Raman spectroscopy.
Lin J, Chen R, Feng S, Li Y, Huang Z, Xie S, Yu Y, Cheng M, Zeng H., Biosens Bioelectron 25(2), 2009
PMID: 19699079
Chemopreventive efficacy of piperine in 7,12-dimethyl benz [a] anthracene (DMBA)-induced hamster buccal pouch carcinogenesis: an FT-IR study.
Krishnakumar N, Manoharan S, Palaniappan PR, Venkatachalam P, Manohar MG., Food Chem Toxicol 47(11), 2009
PMID: 19733613
Differentiation of healthy brain tissue and tumors using surface-enhanced Raman scattering.
Aydin O, Altaş M, Kahraman M, Bayrak OF, Culha M., Appl Spectrosc 63(10), 2009
PMID: 19843358
Characterization of yeast species using surface-enhanced Raman scattering.
Sayin I, Kahraman M, Sahin F, Yurdakul D, Culha M., Appl Spectrosc 63(11), 2009
PMID: 19891836
Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry.
Camp CH, Yegnanarayanan S, Eftekhar AA, Sridhar H, Adibi A., Opt Express 17(25), 2009
PMID: 20052214
Chapter 10: Infrared and Raman microscopy in cell biology.
Matthäus C, Bird B, Miljković M, Chernenko T, Romeo M, Diem M., Methods Cell Biol 89(), 2008
PMID: 19118679
Growth, differentiation, and biochemical signatures of rhesus monkey mesenchymal stem cells.
Kim BS, Lee CC, Christensen JE, Huser TR, Chan JW, Tarantal AF., Stem Cells Dev 17(1), 2008
PMID: 18211228
Spectroscopical and mechanical characterization of normal and thalassemic red blood cells by Raman Tweezers.
De Luca AC, Rusciano G, Ciancia R, Martinelli V, Pesce G, Rotoli B, Selvaggi L, Sasso A., Opt Express 16(11), 2008
PMID: 18545504
An integrated optofluidic platform for Raman-activated cell sorting.
Lau AY, Lee LP, Chan JW., Lab Chip 8(7), 2008
PMID: 18584087
Optical tweezers for single cells.
Zhang H, Liu KK., J R Soc Interface 5(24), 2008
PMID: 18381254
Dietary flavonoids inhibit the anticancer effects of the proteasome inhibitor bortezomib.
Liu FT, Agrawal SG, Movasaghi Z, Wyatt PB, Rehman IU, Gribben JG, Newland AC, Jia L., Blood 112(9), 2008
PMID: 18633129
Raman spectroscopic characterization of necrotic cell death.
Kunapareddy N, Freyer JP, Mourant JR., J Biomed Opt 13(5), 2008
PMID: 19021382
Single-cell research: what determines its feasibility?
Sabelnikov A, Kempf CR., Anal Biochem 383(2), 2008
PMID: 18814839
Spectral monitoring of surfactant clearance during alveolar epithelial type II cell differentiation.
Swain RJ, Kemp SJ, Goldstraw P, Tetley TD, Stevens MM., Biophys J 95(12), 2008
PMID: 18820234
Chemical probing of single cancer cells with gold nanoaggregates by surface-enhanced Raman scattering.
Tang HW, Yang XB, Kirkham J, Smith DA., Appl Spectrosc 62(10), 2008
PMID: 18926013
Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers.
Harvey TJ, Faria EC, Henderson A, Gazi E, Ward AD, Clarke NW, Brown MD, Snook RD, Gardner P., J Biomed Opt 13(6), 2008
PMID: 19123651
Raman Tweezers as a Diagnostic Tool of Hemoglobin-Related Blood Disorders.
Rusciano G, De Luca AC, Pesce G, Sasso A., Sensors (Basel) 8(12), 2008
PMID: 27873960
IR microspectroscopy: potential applications in cervical cancer screening.
Walsh MJ, German MJ, Singh M, Pollock HM, Hammiche A, Kyrgiou M, Stringfellow HF, Paraskevaidis E, Martin-Hirsch PL, Martin FL., Cancer Lett 246(1-2), 2007
PMID: 16713674
Intracellular degradation of microspheres based on cross-linked dextran hydrogels or amphiphilic block copolymers: a comparative raman microscopy study.
van Manen HJ, van Apeldoorn AA, Verrijk R, van Blitterswijk CA, Otto C., Int J Nanomedicine 2(2), 2007
PMID: 17722552
Monitoring dynamic protein expression in living E. coli. Bacterial cells by laser tweezers Raman spectroscopy.
Chan JW, Winhold H, Corzett MH, Ulloa JM, Cosman M, Balhorn R, Huser T., Cytometry A 71(7), 2007
PMID: 17458881
Metabolic fingerprinting as a diagnostic tool.
Ellis DI, Dunn WB, Griffin JL, Allwood JW, Goodacre R., Pharmacogenomics 8(9), 2007
PMID: 17924839
Early detection of cervical neoplasia by Raman spectroscopy.
Jess PR, Smith DD, Mazilu M, Dholakia K, Riches AC, Herrington CS., Int J Cancer 121(12), 2007
PMID: 17724716

36 References

Daten bereitgestellt von Europe PubMed Central.

Near-infrared Raman spectroscopy for optical diagnosis of lung cancer.
Huang Z, McWilliams A, Lui H, McLean DI, Lam S, Zeng H., Int. J. Cancer 107(6), 2003
PMID: 14601068
In vivo detection of dysplastic tissue by Raman spectroscopy.
Bakker Schut TC, Witjes MJ, Sterenborg HJ, Speelman OC, Roodenburg JL, Marple ET, Bruining HA, Puppels GJ., Anal. Chem. 72(24), 2000
PMID: 11140770
Micro-Raman spectroscopy for optical pathology of oral squamous cell carcinoma.
Krishna CM, Sockalingum GD, Kurien J, Rao L, Venteo L, Pluot M, Manfait M, Kartha VB., Appl Spectrosc 58(9), 2004
PMID: 15479531
The use of Raman spectroscopy to identify and characterize transitional cell carcinoma in vitro.
Crow P, Uff JS, Farmer JA, Wright MP, Stone N., BJU Int. 93(9), 2004
PMID: 15180613
Raman spectroscopy of normal and diseased human breast tissues.
Frank CJ, McCreery RL, Redd DC., Anal. Chem. 67(5), 1995
PMID: 7762814






Raman spectroscopy for early detection of laryngeal malignancy: preliminary results.
Stone N, Stavroulaki P, Kendall C, Birchall M, Barr H., Laryngoscope 110(10 Pt 1), 2000
PMID: 11037840




Cyclosporin A directly inhibits human B-cell proliferation by more than a single mechanism.
Hannam-Harris AC, Taylor DS, Nowell PC., J. Leukoc. Biol. 38(2), 1985
PMID: 3875675
Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy.
Chan JW, Esposito AP, Talley CE, Hollars CW, Lane SM, Huser T., Anal. Chem. 76(3), 2004
PMID: 14750852

Nonresonant confocal Raman imaging of DNA and protein distribution in apoptotic cells.
Uzunbajakava N, Lenferink A, Kraan Y, Volokhina E, Vrensen G, Greve J, Otto C., Biophys. J. 84(6), 2003
PMID: 12770902

Quantitative optical spectroscopy for tissue diagnosis.
Richards-Kortum R, Sevick-Muraca E., Annu Rev Phys Chem 47(), 1996
PMID: 8930102
Nonresonant Raman imaging of protein distribution in single human cells.
Uzunbajakava N, Lenferink A, Kraan Y, Willekens B, Vrensen G, Greve J, Otto C., Biopolymers 72(1), 2003
PMID: 12400086

In situ non-invasive spectral discrimination between bone cell phenotypes used in tissue engineering.
Notingher I, Jell G, Lohbauer U, Salih V, Hench LL., J. Cell. Biochem. 92(6), 2004
PMID: 15258901

Study of chronic lymphocytic leukemia cells by FT-IR spectroscopy and cluster analysis.
Schultz CP, Liu K, Johnston JB, Mantsch HH., Leuk. Res. 20(8), 1996
PMID: 8913318

Prognostic importance of blast cell DNA content in childhood acute lymphoblastic leukemia.
Look AT, Roberson PK, Williams DL, Rivera G, Bowman WP, Pui CH, Ochs J, Abromowitch M, Kalwinsky D, Dahl GV., Blood 65(5), 1985
PMID: 3158360

Raman spectroscopy of DNA and proteins.
Peticolas WL., Meth. Enzymol. 246(), 1995
PMID: 7538626


Melting and premelting phenomenon in DNA by laser Raman scattering.
Erfurth SC, Peticolas WL., Biopolymers 14(2), 1975
PMID: 1174661

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

PMID: 16239327
PubMed | Europe PMC

Suchen in

Google Scholar