Raman spectroscopy and microscopy of individual cells and cellular components (Review)

Chan J, Fore S, Wachsman-Hogiu S, Huser T (2008)
Laser & Photonics Review 2(5): 325-349.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Chan, James; Fore, Samantha; Wachsman-Hogiu, Sebastian; Huser, ThomasUniBi
Abstract / Bemerkung
Raman spectroscopy provides the unique opportunity to nondestructively analyze chemical concentrations in individual cells on the submicrometer length scale without the need for optical labels. This enables the rapid assessment of cellular biochemistry inside living cells, and it allows for their continued analysis. Here, we review recent developments in the analysis of single cells, subcellular compartments, and chemical imaging based on Raman spectroscopy. Spontaneous Raman spectroscopy provides for the full spectral assessment of cellular biochemistry, while coherent Raman techniques, such as coherent anti-Stokes Raman scattering is primarily used as an imaging tool comparable to confocal fluorescence microscopy. These techniques are complemented by surface-enhanced Raman spectroscopy, which provides higher sensitivity and local specificity, and also extends the techniques to chemical indicators, i.e. pH sensing. We review the strengths and weaknesses of each technique, demonstrate some of their applications and discuss their potential for future research in cell biology and biomedicine.
Stichworte
laser tweezers; Raman spectroscopy; coherent anti-Stokes Raman; scattering; CARS microscopy; surface-enhanced Raman spectroscopy; SERS; nanoparticles; time-correlated single-photon counting; microscopy; single-cell
Erscheinungsjahr
2008
Zeitschriftentitel
Laser & Photonics Review
Band
2
Ausgabe
5
Seite(n)
325-349
ISSN
1863-8880
eISSN
1863-8899
Page URI
https://pub.uni-bielefeld.de/record/2330501

Zitieren

Chan J, Fore S, Wachsman-Hogiu S, Huser T. Raman spectroscopy and microscopy of individual cells and cellular components (Review). Laser & Photonics Review. 2008;2(5):325-349.
Chan, J., Fore, S., Wachsman-Hogiu, S., & Huser, T. (2008). Raman spectroscopy and microscopy of individual cells and cellular components (Review). Laser & Photonics Review, 2(5), 325-349. doi:10.1002/lpor.200810012
Chan, J., Fore, S., Wachsman-Hogiu, S., and Huser, T. (2008). Raman spectroscopy and microscopy of individual cells and cellular components (Review). Laser & Photonics Review 2, 325-349.
Chan, J., et al., 2008. Raman spectroscopy and microscopy of individual cells and cellular components (Review). Laser & Photonics Review, 2(5), p 325-349.
J. Chan, et al., “Raman spectroscopy and microscopy of individual cells and cellular components (Review)”, Laser & Photonics Review, vol. 2, 2008, pp. 325-349.
Chan, J., Fore, S., Wachsman-Hogiu, S., Huser, T.: Raman spectroscopy and microscopy of individual cells and cellular components (Review). Laser & Photonics Review. 2, 325-349 (2008).
Chan, James, Fore, Samantha, Wachsman-Hogiu, Sebastian, and Huser, Thomas. “Raman spectroscopy and microscopy of individual cells and cellular components (Review)”. Laser & Photonics Review 2.5 (2008): 325-349.