Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans

Wachsmann-Hogiu S, Weeks T, Huser T (2009)
Current Opinion in Biotechnology 20(1): 63-73.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Wachsmann-Hogiu, Sebastian; Weeks, Tyler; Huser, ThomasUniBi
Abstract / Bemerkung
The gold standard for clinical diagnostics of tissues is immunofluorescence staining. Toxicity of many fluorescent dyes precludes their application in vivo. Raman spectroscopy, a chemically specific, label-free diagnostic technique, is rapidly gaining acceptance as a powerful alternative. It has the ability to probe the chemical composition of biological materials in a non-destructive and mostly non-perturbing manner. We review the most recent developments in Raman spectroscopy in the life sciences, detailing advances in technology that have improved the ability to screen for diseases. Its role in the monitoring of biological function and mapping the cellular chemical microenvironment will be discussed. Applications including endoscopy, surface-enhanced Raman scattering (SERS), and coherent Raman scattering (CRS) will be reviewed.
Current Opinion in Biotechnology
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Wachsmann-Hogiu S, Weeks T, Huser T. Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans. Current Opinion in Biotechnology. 2009;20(1):63-73.
Wachsmann-Hogiu, S., Weeks, T., & Huser, T. (2009). Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans. Current Opinion in Biotechnology, 20(1), 63-73.
Wachsmann-Hogiu, Sebastian, Weeks, Tyler, and Huser, Thomas. 2009. “Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans”. Current Opinion in Biotechnology 20 (1): 63-73.
Wachsmann-Hogiu, S., Weeks, T., and Huser, T. (2009). Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans. Current Opinion in Biotechnology 20, 63-73.
Wachsmann-Hogiu, S., Weeks, T., & Huser, T., 2009. Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans. Current Opinion in Biotechnology, 20(1), p 63-73.
S. Wachsmann-Hogiu, T. Weeks, and T. Huser, “Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans”, Current Opinion in Biotechnology, vol. 20, 2009, pp. 63-73.
Wachsmann-Hogiu, S., Weeks, T., Huser, T.: Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans. Current Opinion in Biotechnology. 20, 63-73 (2009).
Wachsmann-Hogiu, Sebastian, Weeks, Tyler, and Huser, Thomas. “Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans”. Current Opinion in Biotechnology 20.1 (2009): 63-73.

51 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Applications of SNAP-tag technology in skin cancer therapy.
Padayachee ER, Adeola HA, Van Wyk JC, Nsole Biteghe FA, Chetty S, Khumalo NP, Barth S., Health Sci Rep 2(2), 2019
PMID: 30809593
Use of Surface-Enhanced Raman Scattering (SERS) Probes to Detect Fatty Acid Receptor Activity in a Microfluidic Device.
Zhang H, Zhang W, Xiao L, Liu Y, Gilbertson TA, Zhou A., Sensors (Basel) 19(7), 2019
PMID: 30965560
In-vivo Raman spectroscopy: from basics to applications.
Cordero E, Latka I, Matthäus C, Schie I, Popp J., J Biomed Opt 23(7), 2018
PMID: 29956506
Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips.
Pilát Z, Kizovský M, Ježek J, Krátký S, Sobota J, Šiler M, Samek O, Buryška T, Vaňáček P, Damborský J, Prokop Z, Zemánek P., Sensors (Basel) 18(10), 2018
PMID: 30249041
A noninvasive cancer detection strategy based on gold nanoparticle surface-enhanced raman spectroscopy of urinary modified nucleosides isolated by affinity chromatography.
Feng S, Zheng Z, Xu Y, Lin J, Chen G, Weng C, Lin D, Qiu S, Cheng M, Huang Z, Wang L, Chen R, Xie S, Zeng H., Biosens Bioelectron 91(), 2017
PMID: 28103517
Combined Raman and autofluorescence ex vivo diagnostics of skin cancer in near-infrared and visible regions.
Bratchenko IA, Artemyev DN, Myakinin OO, Khristoforova YA, Moryatov AA, Kozlov SV, Zakharov VP., J Biomed Opt 22(2), 2017
PMID: 28205679
Thin silica shell coated Ag assembled nanostructures for expanding generality of SERS analytes.
Cha MG, Kim HM, Kang YL, Lee M, Kang H, Kim J, Pham XH, Kim TH, Hahm E, Lee YS, Jeong DH, Jun BH., PLoS One 12(6), 2017
PMID: 28570633
Leukemia cells detection based on electroporation assisted surface-enhanced Raman scattering.
Yu Y, Lin J, Lin D, Feng S, Chen W, Huang Z, Huang H, Chen R., Biomed Opt Express 8(9), 2017
PMID: 28966850
Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells.
Gualerzi A, Niada S, Giannasi C, Picciolini S, Morasso C, Vanna R, Rossella V, Masserini M, Bedoni M, Ciceri F, Bernardo ME, Brini AT, Gramatica F., Sci Rep 7(1), 2017
PMID: 28852131
Sorting of Single Biomolecules based on Fourier Polar Representation of Surface Enhanced Raman Spectra.
Leray A, Brulé T, Buret M, Colas des Francs G, Bouhelier A, Dereux A, Finot E., Sci Rep 6(), 2016
PMID: 26833130
Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy.
Oliveira Mendes Td, Pinto LP, Santos LD, Tippavajhala VK, Téllez Soto CA, Martin AA., J Biomed Opt 21(7), 2016
PMID: 27411080
Label-free visualization of cholesteatoma in the mastoid and tympanic membrane using CARS microscopy.
Zou J, Isomäki A, Hirvonen T, Aarnisalo A, Jero J, Pyykkö I., J Otol 11(3), 2016
PMID: 29937821
Label-free monitoring of tissue biochemistry following traumatic brain injury using Raman spectroscopy.
Surmacki JM, Ansel-Bollepalli L, Pischiutta F, Zanier ER, Ercole A, Bohndiek SE., Analyst 142(1), 2016
PMID: 27905576
Early discrimination of nasopharyngeal carcinoma based on tissue deoxyribose nucleic acid surface-enhanced Raman spectroscopy analysis.
Qiu S, Li C, Lin J, Xu Y, Lu J, Huang Q, Zou C, Chen C, Xiao N, Lin D, Chen R, Pan J, Feng S., J Biomed Opt 21(12), 2016
PMID: 27936269
The many facets of Raman spectroscopy for biomedical analysis.
Krafft C, Popp J., Anal Bioanal Chem 407(3), 2015
PMID: 25428454
Label-free imaging and identification of typical cells of acute myeloid leukaemia and myelodysplastic syndrome by Raman microspectroscopy.
Vanna R, Ronchi P, Lenferink AT, Tresoldi C, Morasso C, Mehn D, Bedoni M, Picciolini S, Terstappen LW, Ciceri F, Otto C, Gramatica F., Analyst 140(4), 2015
PMID: 25568900
Detection of vancomycin resistances in enterococci within 3 ½ hours.
Schröder UC, Beleites C, Assmann C, Glaser U, Hübner U, Pfister W, Fritzsche W, Popp J, Neugebauer U., Sci Rep 5(), 2015
PMID: 25645753
Pilot study for detection of early changes in tissue associated with heterotopic ossification: moving toward clinical use of Raman spectroscopy.
Harris M, Cilwa K, Elster EA, Potter BK, Forsberg JA, Crane NJ., Connect Tissue Res 56(2), 2015
PMID: 25738521
Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content.
Smith ZJ, Lee C, Rojalin T, Carney RP, Hazari S, Knudson A, Lam K, Saari H, Ibañez EL, Viitala T, Laaksonen T, Yliperttula M, Wachsmann-Hogiu S., J Extracell Vesicles 4(), 2015
PMID: 26649679
Histology verification demonstrates that biospectroscopy analysis of cervical cytology identifies underlying disease more accurately than conventional screening: removing the confounder of discordance.
Gajjar K, Ahmadzai AA, Valasoulis G, Trevisan J, Founta C, Nasioutziki M, Loufopoulos A, Kyrgiou M, Stasinou SM, Karakitsos P, Paraskevaidis E, Da Gama-Rose B, Martin-Hirsch PL, Martin FL., PLoS One 9(1), 2014
PMID: 24404130
Simple SERS substrates: powerful, portable, and full of potential.
Betz JF, Yu WW, Cheng Y, White IM, Rubloff GW., Phys Chem Chem Phys 16(6), 2014
PMID: 24366393
Raman spectroscopy of microbial pigments.
Jehlička J, Edwards HG, Oren A., Appl Environ Microbiol 80(11), 2014
PMID: 24682303
Under- and over-water halves of Gyrinidae beetle eyes harbor different corneal nanocoatings providing adaptation to the water and air environments.
Blagodatski A, Kryuchkov M, Sergeev A, Klimov AA, Shcherbakov MR, Enin GA, Katanaev VL., Sci Rep 4(), 2014
PMID: 25103074
Trace cancer biomarker quantification using polystyrene-functionalized gold nanorods.
Wu J, Li W, Hajisalem G, Lukach A, Kumacheva E, Hof F, Gordon R., Biomed Opt Express 5(12), 2014
PMID: 25574423
In situ fabrication of 3D Ag@ZnO nanostructures for microfluidic surface-enhanced Raman scattering systems.
Xie Y, Yang S, Mao Z, Li P, Zhao C, Cohick Z, Huang PH, Huang TJ., ACS Nano 8(12), 2014
PMID: 25402207
Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology.
Jehlička J, Edwards HG, Osterrothová K, Novotná J, Nedbalová L, Kopecký J, Němec I, Oren A., Philos Trans A Math Phys Eng Sci 372(2030), 2014
PMID: 25368348
Stem cell tracking with optically active nanoparticles.
Gao Y, Cui Y, Chan JK, Xu C., Am J Nucl Med Mol Imaging 3(3), 2013
PMID: 23638335
Illuminating disease and enlightening biomedicine: Raman spectroscopy as a diagnostic tool.
Ellis DI, Cowcher DP, Ashton L, O'Hagan S, Goodacre R., Analyst 138(14), 2013
PMID: 23722248
A small animal Raman instrument for rapid, wide-area, spectroscopic imaging.
Bohndiek SE, Wagadarikar A, Zavaleta CL, Van de Sompel D, Garai E, Jokerst JV, Yazdanfar S, Gambhir SS., Proc Natl Acad Sci U S A 110(30), 2013
PMID: 23821752
The role of asbestos morphology on their cellular toxicity: an in vitro 3D Raman/Rayleigh imaging study.
Yao S, Chen HH, Harte E, Ventura GD, Petibois C., Anal Bioanal Chem 405(27), 2013
PMID: 23846589
A molecular imaging primer: modalities, imaging agents, and applications.
James ML, Gambhir SS., Physiol Rev 92(2), 2012
PMID: 22535898
Label-free Raman spectroscopy for accessing intracellular anticancer drug release on gold nanoparticles.
Ock KS, Ganbold EO, Park J, Cho K, Joo SW, Lee SY., Analyst 137(12), 2012
PMID: 22569426
Cell imaging by graphene oxide based on surface enhanced Raman scattering.
Liu Q, Wei L, Wang J, Peng F, Luo D, Cui R, Niu Y, Qin X, Liu Y, Sun H, Yang J, Li Y., Nanoscale 4(22), 2012
PMID: 23070130
Dual-mode probe based on mesoporous silica coated gold nanorods for targeting cancer cells.
Wang Z, Zong S, Yang J, Li J, Cui Y., Biosens Bioelectron 26(6), 2011
PMID: 21177092
Gastric cancer detection based on blood plasma surface-enhanced Raman spectroscopy excited by polarized laser light.
Feng S, Chen R, Lin J, Pan J, Wu Y, Li Y, Chen J, Zeng H., Biosens Bioelectron 26(7), 2011
PMID: 21227679
Intra-operative optical diagnostics with vibrational spectroscopy.
Stelling A, Salzer R, Kirsch M, Sobottka SB, Geiger K, Koch E, Schackert G, Steiner G., Anal Bioanal Chem 400(9), 2011
PMID: 21533800
Colorectal cancer detection by gold nanoparticle based surface-enhanced Raman spectroscopy of blood serum and statistical analysis.
Lin D, Feng S, Pan J, Chen Y, Lin J, Chen G, Xie S, Zeng H, Chen R., Opt Express 19(14), 2011
PMID: 21747512
Molecular imaging with SERS-active nanoparticles.
Zhang Y, Hong H, Myklejord DV, Cai W., Small 7(23), 2011
PMID: 21932216
Signal generation and Raman-resonant imaging by non-degenerate four-wave mixing under tight focusing conditions.
Weeks T, Schie IW, Wachsmann-Hogiu S, Huser T., J Biophotonics 3(3), 2010
PMID: 19953535
Plasmonic properties of Fischer's patterns: polarization effects.
Galarreta BC, Harté E, Marquestaut N, Norton PR, Lagugné-Labarthet F., Phys Chem Chem Phys 12(25), 2010
PMID: 20448875
Imaging with Raman spectroscopy.
Zhang Y, Hong H, Cai W., Curr Pharm Biotechnol 11(6), 2010
PMID: 20497112
Raman microscopy based on doubly-resonant four-wave mixing (DR-FWM).
Weeks T, Wachsmann-Hogiu S, Huser T., Opt Express 17(19), 2009
PMID: 19770922

50 References

Daten bereitgestellt von Europe PubMed Central.

Improving nanoprobes using surface-enhanced Raman scattering from 30-nm hollow gold particles.
Schwartzberg AM, Oshiro TY, Zhang JZ, Huser T, Talley CE., Anal. Chem. 78(13), 2006
PMID: 16808490
Nanoparticle probes with surface enhanced Raman spectroscopic tags for cellular cancer targeting.
Kim JH, Kim JS, Choi H, Lee SM, Jun BH, Yu KN, Kuk E, Kim YK, Jeong DH, Cho MH, Lee YS., Anal. Chem. 78(19), 2006
PMID: 17007522
Multiplex detection and imaging of cancer markers based on surface-enhanced raman spectroscopic nanoparticle probes (SERS Dots). Nanomedicine-Nanotechnology
Kim JH, Lee SM, Jun BH, Choi HJ, Kim JS, Cho MH, Kim YK, Jeong DH, Lee YS., 2007
In vivo tumor targeting and spectroscopic detection with surface-enhanced Raman nanoparticle tags.
Qian X, Peng XH, Ansari DO, Yin-Goen Q, Chen GZ, Shin DM, Yang L, Young AN, Wang MD, Nie S., Nat. Biotechnol. 26(1), 2007
PMID: 18157119
Raman signal enhancement in deep spectroscopy of turbid media.
Matousek P., Appl Spectrosc 61(8), 2007
PMID: 17716403
Heterodyne coherent anti-Stokes Raman scattering (CARS) imaging.
Potma EO, Evans CL, Xie XS., Opt Lett 31(2), 2006
PMID: 16441043
Discriminating basal cell carcinoma from perilesional skin using high wave-number Raman spectroscopy
Nijssen A, Maquelin K, Santos LF, Caspers PJ, Schut TCB, Hollander JCD, Neumann MHA, Puppels GJ., 2007
Development of a handheld Raman microspectrometer for clinical dermatologic applications.
Lieber C, Mahadevan-Jansen A., Opt Express 15(19), 2007
PMID: 19547550
Development and preliminary results of an endoscopic Raman probe for potential in vivo diagnosis of lung cancers.
Short MA, Lam S, McWilliams A, Zhao J, Lui H, Zeng H., Opt Lett 33(7), 2008
PMID: 18382526
Detection of Human Viral RNA via a Combined Fluorescence and SERS Molecular Beacon Assay
Sha M, Penn S, Freeman G, Doering W., 2007
Aptamer-Based SERRS Sensor for Thrombin Detection
Cho H, Baker BR, Wachsmann-Hogiu S, Pagba CV, Laurence TA, Lane SM, Lee LP, Tok JBH., 2008
Surface-enhanced Raman scattering spectroscopy as a sensitive and selective technique for the detection of folic acid in water and human serum.
Stokes RJ, McBride E, Wilson CG, Girkin JM, Smith WE, Graham D., Appl Spectrosc 62(4), 2008
PMID: 18416893
Detection of melamine in gluten, chicken feed, and processed foods using surface enhanced Raman spectroscopy and HPLC.
Lin M, He L, Awika J, Yang L, Ledoux DR, Li H, Mustapha A., J. Food Sci. 73(8), 2008
PMID: 19019134
SERS platforms for high density DNA arrays.
Green M, Liu FM, Cohen L, Kollensperger P, Cass T., Faraday Discuss. 132(), 2006
PMID: 16833122
Composite organic-inorganic nanoparticles as Raman labels for tissue analysis.
Sun L, Sung KB, Dentinger C, Lutz B, Nguyen L, Zhang J, Qin H, Yamakawa M, Cao M, Lu Y, Chmura AJ, Zhu J, Su X, Berlin AA, Chan S, Knudsen B., Nano Lett. 7(2), 2007
PMID: 17298000
Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer).
Pal A, Isola NR, Alarie JP, Stokes DL, Vo-Dinh T., Faraday Discuss. 132(), 2006
PMID: 16833124
Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells.
Chan JW, Taylor DS, Zwerdling T, Lane SM, Ihara K, Huser T., Biophys. J. 90(2), 2005
PMID: 16239327
Nondestructive identification of individual leukemia cells by laser trapping Raman spectroscopy.
Chan JW, Taylor DS, Lane SM, Zwerdling T, Tuscano J, Huser T., Anal. Chem. 80(6), 2008
PMID: 18260656
Monitoring neurotransmitter release using surface-enhanced Raman spectroscopy.
Dijkstra RJ, Scheenen WJ, Dam N, Roubos EW, ter Meulen JJ., J. Neurosci. Methods 159(1), 2006
PMID: 16919757
Biological pH sensing based on surface enhanced Raman scattering through a 2-aminothiophenol-silver probe.
Wang Z, Bonoiu A, Samoc M, Cui Y, Prasad PN., Biosens Bioelectron 23(6), 2007
PMID: 17996441
All-optical nanoscale pH meter.
Bishnoi SW, Rozell CJ, Levin CS, Gheith MK, Johnson BR, Johnson DH, Halas NJ., Nano Lett. 6(8), 2006
PMID: 16895357
Raman spectroscopy of parathyroid tissue pathology.
Das K, Stone N, Kendall C, Fowler C, Christie-Brown J., Lasers Med Sci 21(4), 2006
PMID: 17024320
Discrimination between nontumor bladder tissue and tumor by Raman spectroscopy
de BWD, Bakker TCS, Maquelin K, van T, Bangma CH, Kok D, Puppels GJ., 2006
Discrimination of normal, benign, and malignant breast tissues by Raman spectroscopy.
Chowdary MV, Kumar KK, Kurien J, Mathew S, Krishna CM., Biopolymers 83(5), 2006
PMID: 16897764
Chemically-selective imaging of brain structures with CARS microscopy.
Evans CL, Xu X, Kesari S, Xie XS, Wong ST, Young GS., Opt Express 15(19), 2007
PMID: 19547572
Raman nanoparticle probes for antibody-based protein detection in tissues.
Lutz B, Dentinger C, Sun L, Nguyen L, Zhang J, Chmura A, Allen A, Chan S, Knudsen B., J. Histochem. Cytochem. 56(4), 2007
PMID: 18071064
In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy.
Lieber CA, Majumder SK, Ellis DL, Billheimer DD, Mahadevan-Jansen A., Lasers Surg Med 40(7), 2008
PMID: 18727020
Real-time Raman system for in vivo disease diagnosis
Motz JT, Gandhi SJ, Scepanovic OR, Haka AS, Kramer JR, Dasari RR, Feld MS., 2005
In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque.
Motz JT, Fitzmaurice M, Miller A, Gandhi SJ, Haka AS, Galindo LH, Dasari RR, Kramer JR, Feld MS., J Biomed Opt 11(2), 2006
PMID: 16674178
Detection of morphological markers of vulnerable atherosclerotic plaque using multimodal spectroscopy.
Scepanovic OR, Fitzmaurice M, Gardecki JA, Angheloiu GO, Awasthi S, Motz JT, Kramer JR, Dasari RR, Feld MS., J Biomed Opt 11(2), 2006
PMID: 16674182
Diagnosing breast cancer by using Raman spectroscopy.
Haka AS, Shafer-Peltier KE, Fitzmaurice M, Crowe J, Dasari RR, Feld MS., Proc. Natl. Acad. Sci. U.S.A. 102(35), 2005
PMID: 16116095
In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy.
Haka AS, Volynskaya Z, Gardecki JA, Nazemi J, Lyons J, Hicks D, Fitzmaurice M, Dasari RR, Crowe JP, Feld MS., Cancer Res. 66(6), 2006
PMID: 16540686
Noninvasive molecular imaging of small living subjects using Raman spectroscopy.
Keren S, Zavaleta C, Cheng Z, de la Zerda A, Gheysens O, Gambhir SS., Proc. Natl. Acad. Sci. U.S.A. 105(15), 2008
PMID: 18378895
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
Evans CL, Potma EO, Puoris'haag M, Cote D, Lin CP, Xie XS., Proc. Natl. Acad. Sci. U.S.A. 102(46), 2005
PMID: 16263923
In vivo coherent anti-Stokes Raman scattering imaging of sciatic nerve tissue
Huff TB, Cheng JX., 2007
Towards CARS Endoscopy.
Legare F, Evans CL, Ganikhanov F, Xie XS., Opt Express 14(10), 2006
PMID: 19516594

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