Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy

Chan JW, Lieu DK, Huser T, Li RA (2009)
Analytical Chemistry 81(4): 1324-1331.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Chan, James W.; Lieu, Deborah K.; Huser, ThomasUniBi ; Li, Ronald A.
Abstract / Bemerkung
Self-renewable, pluripotent human embryonic stem cells (hESCs) can be differentiated into cardiomyocytes (CMs), providing an unlimited source of cells for transplantation therapies. However, unlike certain cell lineages such as hematopoietic cells, CMs lack specific surface markers for convenient identification, physical separation, and enrichment Identification by immunostaining of cardiac-specific proteins such as troponin requires permeabilization, which renders the cells unviable and nonrecoverable. Ectopic expression of a reporter protein under the transcriptional control of a heart-specific promoter for identifying hESC-derived CMs (hESC-CMs) is useful for research but complicates potential clinical applications. The practical detection and removal of undifferentiated hESCs in a graft, which may lead to tumors, is also critical. Here, we demonstrate a nondestructive, label-free optical method based on Raman scattering to interrogate the intrinsic biochemical signatures of individual hESCs and their cardiac derivatives, allowing cells to be identified and classified. By combination of the Raman spectroscopic data with multivariate statistical analysis, our results indicate that hESCs, human fetal left ventricular CMs, and hESC-CMs can be identified by their intrinsic biochemical characteristics with an accuracy of 96%, 98%, and 66%, respectively. The present study lays the groundwork for developing a systematic and automated method for the noninvasive and label-free sorting of (i) high-quality hESCs for expansion and (ii) ex vivo CMs (derived from embryonic or adult stem cells) for cell-based heart therapies.
Erscheinungsjahr
2009
Zeitschriftentitel
Analytical Chemistry
Band
81
Ausgabe
4
Seite(n)
1324-1331
ISSN
0003-2700
eISSN
1520-6882
Page URI
https://pub.uni-bielefeld.de/record/2330481

Zitieren

Chan JW, Lieu DK, Huser T, Li RA. Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy. Analytical Chemistry. 2009;81(4):1324-1331.
Chan, J. W., Lieu, D. K., Huser, T., & Li, R. A. (2009). Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy. Analytical Chemistry, 81(4), 1324-1331. https://doi.org/10.1021/ac801665m
Chan, J. W., Lieu, D. K., Huser, T., and Li, R. A. (2009). Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy. Analytical Chemistry 81, 1324-1331.
Chan, J.W., et al., 2009. Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy. Analytical Chemistry, 81(4), p 1324-1331.
J.W. Chan, et al., “Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy”, Analytical Chemistry, vol. 81, 2009, pp. 1324-1331.
Chan, J.W., Lieu, D.K., Huser, T., Li, R.A.: Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy. Analytical Chemistry. 81, 1324-1331 (2009).
Chan, James W., Lieu, Deborah K., Huser, Thomas, and Li, Ronald A. “Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy”. Analytical Chemistry 81.4 (2009): 1324-1331.

92 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

High-throughput label-free molecular fingerprinting flow cytometry.
Hiramatsu K, Ideguchi T, Yonamine Y, Lee S, Luo Y, Hashimoto K, Ito T, Hase M, Park JW, Kasai Y, Sakuma S, Hayakawa T, Arai F, Hoshino Y, Goda K., Sci Adv 5(1), 2019
PMID: 30746443
Electroconductive Biohybrid Collagen/Pristine Graphene Composite Biomaterials with Enhanced Biological Activity.
Ryan AJ, Kearney CJ, Shen N, Khan U, Kelly AG, Probst C, Brauchle E, Biccai S, Garciarena CD, Vega-Mayoral V, Loskill P, Kerrigan SW, Kelly DJ, Schenke-Layland K, Coleman JN, O'Brien FJ., Adv Mater 30(15), 2018
PMID: 29504165
Visualizing Intrapopulation Hematopoietic Cell Heterogeneity with Self-Organizing Maps of SIMS Data.
Mirshafiee V, Harley BAC, Kraft ML., Tissue Eng Part C Methods 24(6), 2018
PMID: 29652627
Label-free Molecular Imaging and Analysis by Raman Spectroscopy.
Kumamoto Y, Harada Y, Takamatsu T, Tanaka H., Acta Histochem Cytochem 51(3), 2018
PMID: 30083018
Raman spectrum reveals Mesenchymal stem cells inhibiting HL60 cells growth.
Su X, Fang S, Zhang D, Zhang Q, Lu X, Tian J, Fan J, LiyunZhong., Spectrochim Acta A Mol Biomol Spectrosc 177(), 2017
PMID: 28109964
Demonstration of the Protein Involvement in Cell Electropermeabilization using Confocal Raman Microspectroscopy.
Azan A, Untereiner V, Gobinet C, Sockalingum GD, Breton M, Piot O, Mir LM., Sci Rep 7(), 2017
PMID: 28102326
Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System.
Low K, Wong LY, Maldonado M, Manjunath C, Horner CB, Perez M, Myung NV, Nam J., Stem Cell Reports 8(5), 2017
PMID: 28457888
New advances in probing cell-extracellular matrix interactions.
Liu AP, Chaudhuri O, Parekh SH., Integr Biol (Camb) 9(5), 2017
PMID: 28352896
Raman spectroscopy and regenerative medicine: a review.
Ember KJI, Hoeve MA, McAughtrie SL, Bergholt MS, Dwyer BJ, Stevens MM, Faulds K, Forbes SJ, Campbell CJ., NPJ Regen Med 2(), 2017
PMID: 29302348
Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes.
Heraud P, Marzec KM, Zhang QH, Yuen WS, Carroll J, Wood BR., Sci Rep 7(1), 2017
PMID: 28827720
Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells.
Gualerzi A, Niada S, Giannasi C, Picciolini S, Morasso C, Vanna R, Rossella V, Masserini M, Bedoni M, Ciceri F, Bernardo ME, Brini AT, Gramatica F., Sci Rep 7(1), 2017
PMID: 28852131
Two sides of the same coin? Unraveling subtle differences between human embryonic and induced pluripotent stem cells by Raman spectroscopy.
Parrotta E, De Angelis MT, Scalise S, Candeloro P, Santamaria G, Paonessa M, Coluccio ML, Perozziello G, De Vitis S, Sgura A, Coluzzi E, Mollace V, Di Fabrizio EM, Cuda G., Stem Cell Res Ther 8(1), 2017
PMID: 29183402
Histological coherent Raman imaging: a prognostic review.
Cicerone MT, Camp CH., Analyst 143(1), 2017
PMID: 29098226
Non-invasive Chamber-Specific Identification of Cardiomyocytes in Differentiating Pluripotent Stem Cells.
Brauchle E, Knopf A, Bauer H, Shen N, Linder S, Monaghan MG, Ellwanger K, Layland SL, Brucker SY, Nsair A, Schenke-Layland K., Stem Cell Reports 6(2), 2016
PMID: 26777059
Label-free biomarkers of human embryonic stem cell differentiation to hepatocytes.
Tsikritsis D, Shi H, Wang Y, Velugotla S, Sršeň V, Elfick A, Downes A., Cytometry A 89(6), 2016
PMID: 27214589
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells.
Hofemeier AD, Hachmeister H, Pilger C, Schürmann M, Greiner JF, Nolte L, Sudhoff H, Kaltschmidt C, Huser T, Kaltschmidt B., Sci Rep 6(), 2016
PMID: 27225821
Molecular imaging with CARS micro-spectroscopy.
Cicerone M., Curr Opin Chem Biol 33(), 2016
PMID: 27400394
IR and Raman imaging of murine brains from control and ApoE/LDLR(-/-) mice with advanced atherosclerosis.
Kochan K, Chrabaszcz K, Szczur B, Maslak E, Dybas J, Marzec KM., Analyst 141(18), 2016
PMID: 27332112
Label-free optical sensor based on red blood cells laser tweezers Raman spectroscopy analysis for ABO blood typing.
Lin D, Zheng Z, Wang Q, Huang H, Huang Z, Yu Y, Qiu S, Wen C, Cheng M, Feng S., Opt Express 24(21), 2016
PMID: 27828195
Non-label immune cell state prediction using Raman spectroscopy.
Ichimura T, Chiu LD, Fujita K, Machiyama H, Yamaguchi T, Watanabe TM, Fujita H., Sci Rep 6(), 2016
PMID: 27876845
Label-free and non-invasive monitoring of porcine trophoblast derived cells: differentiation in serum and serum-free media.
Li Q, Suasnavas E, Xiao L, Heywood S, Qi X, Zhou A, Isom SC., J Biophotonics 8(8), 2015
PMID: 25250877
Biochemical investigations of human papillomavirus-infected cervical fluids.
Choi S, Park HK, Min GE, Kim YH., Microsc Res Tech 78(3), 2015
PMID: 25545188
Surface plasmon resonance: a label-free tool for cellular analysis.
Zeidan E, Kepley CL, Sayes C, Sandros MG., Nanomedicine (Lond) 10(11), 2015
PMID: 26080702
Raman spectrum reveals the cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis.
Zhang D, Feng Y, Zhang Q, Su X, Lu X, Liu S, Zhong L., Spectrochim Acta A Mol Biomol Spectrosc 141(), 2015
PMID: 25681805
Raman microscopy for cellular investigations--From single cell imaging to drug carrier uptake visualization.
Kann B, Offerhaus HL, Windbergs M, Otto C., Adv Drug Deliv Rev 89(), 2015
PMID: 25728764
Raman spectroscopy for physiological investigations of tissues and cells.
Huser T, Chan J., Adv Drug Deliv Rev 89(), 2015
PMID: 26144996
Time-lapse Raman imaging of osteoblast differentiation.
Hashimoto A, Yamaguchi Y, Chiu LD, Morimoto C, Fujita K, Takedachi M, Kawata S, Murakami S, Tamiya E., Sci Rep 5(), 2015
PMID: 26211729
Radiation-Induced Glycogen Accumulation Detected by Single Cell Raman Spectroscopy Is Associated with Radioresistance that Can Be Reversed by Metformin.
Matthews Q, Isabelle M, Harder SJ, Smazynski J, Beckham W, Brolo AG, Jirasek A, Lum JJ., PLoS One 10(8), 2015
PMID: 26280348
Towards high-throughput microfluidic Raman-activated cell sorting.
Zhang Q, Zhang P, Gou H, Mou C, Huang WE, Yang M, Xu J, Ma B., Analyst 140(18), 2015
PMID: 26225617
Label-free assessment of replicative senescence in mesenchymal stem cells by Raman microspectroscopy.
Bai H, Li H, Han Z, Zhang C, Zhao J, Miao C, Yan S, Mao A, Zhao H, Han Z., Biomed Opt Express 6(11), 2015
PMID: 26601012
Enhanced activity and stability of organophosphorus hydrolase via interaction with an amphiphilic polymer.
Kim M, Gkikas M, Huang A, Kang JW, Suthiwangcharoen N, Nagarajan R, Olsen BD., Chem Commun (Camb) 50(40), 2014
PMID: 24558645
Cellular distribution and cytotoxicity of graphene quantum dots with different functional groups.
Yuan X, Liu Z, Guo Z, Ji Y, Jin M, Wang X., Nanoscale Res Lett 9(1), 2014
PMID: 24597852
Investigating drug induced changes in single, living lymphocytes based on Raman micro-spectroscopy.
Schie IW, Alber L, Gryshuk AL, Chan JW., Analyst 139(11), 2014
PMID: 24756205
Clinico-biochemical investigations of aging effects on normoglycemic and hyperglycemic murine retinal tissues.
Shin JH, Choi JH, Choi S, Jung GB, Park HK, Moon SW., Microsc Res Tech 77(12), 2014
PMID: 25209727
Silicon nanohybrid-based surface-enhanced Raman scattering sensors.
Wang H, Jiang X, Lee ST, He Y., Small 10(22), 2014
PMID: 25243935
Discrimination and classification of liver cancer cells and proliferation states by Raman spectroscopic imaging.
Tolstik T, Marquardt C, Matthäus C, Bergner N, Bielecki C, Krafft C, Stallmach A, Popp J., Analyst 139(22), 2014
PMID: 25271553
Stem cell metabolic and spectroscopic profiling.
Ramm Sander P, Hau P, Koch S, Schütze K, Bogdahn U, Kalbitzer HR, Aigner L., Trends Biotechnol 31(3), 2013
PMID: 23384506
Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy.
Pascut FC, Kalra S, George V, Welch N, Denning C, Notingher I., Biochim Biophys Acta 1830(6), 2013
PMID: 23403134
Characterizing the intracellular distribution of metabolites in intact Chlamydia-infected cells by Raman and two-photon microscopy.
Szaszák M, Chang JC, Leng W, Rupp J, Ojcius DM, Kelley AM., Microbes Infect 15(6-7), 2013
PMID: 23541981
The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy.
Clemens G, Flower KR, Henderson AP, Whiting A, Przyborski SA, Jimenez-Hernandez M, Ball F, Bassan P, Cinque G, Gardner P., Mol Biosyst 9(4), 2013
PMID: 23364809
Optical tweezers for medical diagnostics.
LaFratta CN., Anal Bioanal Chem 405(17), 2013
PMID: 23559336
Stem cell tracking with optically active nanoparticles.
Gao Y, Cui Y, Chan JK, Xu C., Am J Nucl Med Mol Imaging 3(3), 2013
PMID: 23638335
Label-free imaging of mammalian cell nucleoli by Raman microspectroscopy.
Schulze HG, Konorov SO, Piret JM, Blades MW, Turner RF., Analyst 138(12), 2013
PMID: 23636076
Intracellular SERS nanoprobes for distinction of different neuronal cell types.
Huefner A, Kuan WL, Barker RA, Mahajan S., Nano Lett 13(6), 2013
PMID: 23638825
Calcium signalling of human pluripotent stem cell-derived cardiomyocytes.
Li S, Chen G, Li RA., J Physiol 591(21), 2013
PMID: 24018947
Label-free detection of insulin and glucagon within human islets of Langerhans using Raman spectroscopy.
Hilderink J, Otto C, Slump C, Lenferink A, Engelse M, van Blitterswijk C, de Koning E, Karperien M, van Apeldoorn A., PLoS One 8(10), 2013
PMID: 24167603
Molecular pathology via IR and Raman spectral imaging.
Diem M, Mazur A, Lenau K, Schubert J, Bird B, Miljković M, Krafft C, Popp J., J Biophotonics 6(11-12), 2013
PMID: 24311233
Discrimination of primitive endoderm in embryoid bodies by Raman microspectroscopy.
El-Hagrasy MA, Shimizu E, Saito M, Yamaguchi Y, Tamiya E., Anal Bioanal Chem 402(3), 2012
PMID: 22101464
Identification of abnormal stem cells using Raman spectroscopy.
Harkness L, Novikov SM, Beermann J, Bozhevolnyi SI, Kassem M., Stem Cells Dev 21(12), 2012
PMID: 22251070
In vitro uses of human pluripotent stem cell-derived cardiomyocytes.
Matsa E, Denning C., J Cardiovasc Transl Res 5(5), 2012
PMID: 22639342
Label-free hybridoma cell culture quality control by a chip-based impedance flow cytometer.
Pierzchalski A, Hebeisen M, Mittag A, Bocsi J, Di Berardino M, Tarnok A., Lab Chip 12(21), 2012
PMID: 22907524
Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells.
Pascut FC, Goh HT, Welch N, Buttery LD, Denning C, Notingher I., Biophys J 100(1), 2011
PMID: 21190678
Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells.
Pascut FC, Goh HT, George V, Denning C, Notingher I., J Biomed Opt 16(4), 2011
PMID: 21529069
Raman spectroscopy and CARS microscopy of stem cells and their derivatives.
Downes A, Mouras R, Bagnaninchi P, Elfick A., J Raman Spectrosc 42(10), 2011
PMID: 22319014
Optical spectroscopy for noninvasive monitoring of stem cell differentiation.
Downes A, Mouras R, Elfick A., J Biomed Biotechnol 2010(), 2010
PMID: 20182537
Microspectroscopy of spectral biomarkers associated with human corneal stem cells.
Nakamura T, Kelly JG, Trevisan J, Cooper LJ, Bentley AJ, Carmichael PL, Scott AD, Cotte M, Susini J, Martin-Hirsch PL, Kinoshita S, Fullwood NJ, Martin FL., Mol Vis 16(), 2010
PMID: 20520745
Label-free cellular imaging by broadband coherent anti-Stokes Raman scattering microscopy.
Parekh SH, Lee YJ, Aamer KA, Cicerone MT., Biophys J 99(8), 2010
PMID: 20959111
Confocal Raman microspectral imaging (CRMI) of murine stem cell colonies.
Zuser E, Chernenko T, Newmark J, Miljković M, Diem M., Analyst 135(12), 2010
PMID: 20944846
In situ monitoring of adipogenesis with human-adipose-derived stem cells using surface-enhanced Raman spectroscopy.
Moody B, Haslauer CM, Kirk E, Kannan A, Loboa EG, McCarty GS., Appl Spectrosc 64(11), 2010
PMID: 21073790
Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells.
Huser T, Orme CA, Hollars CW, Corzett MH, Balhorn R., J Biophotonics 2(5), 2009
PMID: 19373853
The emergence of biospectroscopy in stem cell research.
Heraud P, Tobin MJ., Stem Cell Res 3(1), 2009
PMID: 19464243

38 References

Daten bereitgestellt von Europe PubMed Central.


Li RA, Moore JC, Tarasova YS, Boheler KR., 2006
Human embryonic stem cell-derived cardiomyocytes for heart therapies.
Siu CW, Moore JC, Li RA., Cardiovasc Hematol Disord Drug Targets 7(2), 2007
PMID: 17584049
Human embryonic stem cells: genetic manipulation on the way to cardiac cell therapies.
Moore JC, van Laake LW, Braam SR, Xue T, Tsang SY, Ward D, Passier R, Tertoolen LL, Li RA, Mummery CL., Reprod. Toxicol. 20(3), 2005
PMID: 15967632
Embryonic stem cell lines derived from human blastocysts.
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM., Science 282(5391), 1998
PMID: 9804556
Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells.
Mummery C, Ward-van Oostwaard D, Doevendans P, Spijker R, van den Brink S, Hassink R, van der Heyden M, Opthof T, Pera M, de la Riviere AB, Passier R, Tertoolen L., Circulation 107(21), 2003
PMID: 12742992
Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes.
Kehat I, Kenyagin-Karsenti D, Snir M, Segev H, Amit M, Gepstein A, Livne E, Binah O, Itskovitz-Eldor J, Gepstein L., J. Clin. Invest. 108(3), 2001
PMID: 11489934
Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells.
Xu C, Police S, Rao N, Carpenter MK., Circ. Res. 91(6), 2002
PMID: 12242268
Electromechanical integration of cardiomyocytes derived from human embryonic stem cells.
Kehat I, Khimovich L, Caspi O, Gepstein A, Shofti R, Arbel G, Huber I, Satin J, Itskovitz-Eldor J, Gepstein L., Nat. Biotechnol. 22(10), 2004
PMID: 15448703
Identification and selection of cardiomyocytes during human embryonic stem cell differentiation.
Huber I, Itzhaki I, Caspi O, Arbel G, Tzukerman M, Gepstein A, Habib M, Yankelson L, Kehat I, Gepstein L., FASEB J. 21(10), 2007
PMID: 17435178
Studying single living cells and chromosomes by confocal Raman microspectroscopy.
Puppels GJ, de Mul FF, Otto C, Greve J, Robert-Nicoud M, Arndt-Jovin DJ, Jovin TM., Nature 347(6290), 1990
PMID: 2205805
Raman spectroscopy in chemical bioanalysis.
Baena JR, Lendl B., Curr Opin Chem Biol 8(5), 2004
PMID: 15450497

Motz JT, Fitzmaurice M, Miller A, Gandhi SJ, Haka AS, Galindo LH, Dasari RR, Kramer JR, Feld MS., 2006
Imaging of atherosclerosis. Raman spectroscopy of atherosclerosis.
van de Poll SW, Romer TJ, Puppels GJ, van der Laarse A., J Cardiovasc Risk 9(5), 2002
PMID: 12394318
Discrimination between nontumor bladder tissue and tumor by Raman spectroscopy.
de Jong BW, Schut TC, Maquelin K, van der Kwast T, Bangma CH, Kok DJ, Puppels GJ., Anal. Chem. 78(22), 2006
PMID: 17105169
Diagnosing breast cancer by using Raman spectroscopy.
Haka AS, Shafer-Peltier KE, Fitzmaurice M, Crowe J, Dasari RR, Feld MS., Proc. Natl. Acad. Sci. U.S.A. 102(35), 2005
PMID: 16116095
Nondestructive identification of individual leukemia cells by laser trapping Raman spectroscopy.
Chan JW, Taylor DS, Lane SM, Zwerdling T, Tuscano J, Huser T., Anal. Chem. 80(6), 2008
PMID: 18260656
In vivo detection of dysplastic tissue by Raman spectroscopy.
Bakker Schut TC, Witjes MJ, Sterenborg HJ, Speelman OC, Roodenburg JL, Marple ET, Bruining HA, Puppels GJ., Anal. Chem. 72(24), 2000
PMID: 11140770

Utzinger U, Heintzelman DL, Mahadevan-Jansen A, Malpica A, Follen M, Richards-Kortum R., 2001

Notingher L, Bisson I, Polak JM, Hench LL., 2004
In situ spectral monitoring of mRNA translation in embryonic stem cells during differentiation in vitro.
Notingher I, Bisson I, Bishop AE, Randle WL, Polak JM, Hench LL., Anal. Chem. 76(11), 2004
PMID: 15167800
Differentiation of individual human mesenchymal stem cells probed by FTIR microscopic imaging.
Krafft C, Salzer R, Seitz S, Ern C, Schieker M., Analyst 132(7), 2007
PMID: 17592583
In situ analysis of living embryonic stem cells by coherent anti-stokes Raman microscopy.
Konorov SO, Glover CH, Piret JM, Bryan J, Schulze HG, Blades MW, Turner RF., Anal. Chem. 79(18), 2007
PMID: 17691751
Growth, differentiation, and biochemical signatures of rhesus monkey mesenchymal stem cells.
Kim BS, Lee CC, Christensen JE, Huser TR, Chan JW, Tarantal AF., Stem Cells Dev. 17(1), 2008
PMID: 18211228
Automated method for subtraction of fluorescence from biological Raman spectra.
Lieber CA, Mahadevan-Jansen A., Appl Spectrosc 57(11), 2003
PMID: 14658149

Wold S, Esbensen K, Geladi P., 1987
Diagnostic potential of near-infrared Raman spectroscopy in the colon: differentiating adenomatous from hyperplastic polyps.
Molckovsky A, Song LM, Shim MG, Marcon NE, Wilson BC., Gastrointest. Endosc. 57(3), 2003
PMID: 12612529
In situ non-invasive spectral discrimination between bone cell phenotypes used in tissue engineering.
Notingher I, Jell G, Lohbauer U, Salih V, Hench LL., J. Cell. Biochem. 92(6), 2004
PMID: 15258901

Notingher L, Jell G, Notingher PL, Bisson I, Tsigkou O, Polak JM, Stevens MM, Hench LL., 2005
Electrophysiological properties of pluripotent human and mouse embryonic stem cells.
Wang K, Xue T, Tsang SY, Van Huizen R, Wong CW, Lai KW, Ye Z, Cheng L, Au KW, Zhang J, Li GR, Lau CP, Tse HF, Li RA., Stem Cells 23(10), 2005
PMID: 16091557
Observation of a single-beam gradient force optical trap for dielectric particles.
Ashkin A, Dziedzic JM, Bjorkholm JE, Chu S., Opt Lett 11(5), 1986
PMID: 19730608
Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light.
Puppels GJ, Olminkhof JH, Segers-Nolten GM, Otto C, de Mul FF, Greve J., Exp. Cell Res. 195(2), 1991
PMID: 2070819
An integrated optofluidic platform for Raman-activated cell sorting.
Lau AY, Lee LP, Chan JW., Lab Chip 8(7), 2008
PMID: 18584087
Microfluidic CARS cytometry.
Wang HW, Bao N, Le TL, Lu C, Cheng JX., Opt Express 16(8), 2008
PMID: 18542688

Cheng JX, Xie XS., 2004

Shi KB, Li P, Liu ZW., 2007
Spectroscopic probing of dynamic changes during stimulation and cell remodeling in the single cardiac myocyte.
Inya-Agha O, Klauke N, Davies T, Smith G, Cooper JM., Anal. Chem. 79(12), 2007
PMID: 17511420

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