Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells

Huser T, Orme CA, Hollars CW, Corzett MH, Balhorn R (2009)
Journal of Biophotonics 2(5): 322-332.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor*in
Huser, ThomasUniBi ; Orme, Christine A.; Hollars, Christopher W.; Corzett, Michele H.; Balhorn, Rod
Abstract / Bemerkung
Healthy human males produce sperm cells of which about 25-40% have abnormal head shapes. Increases in the percentage of sperm exhibiting aberrant sperm head morphologies have been correlated with mate infertility, and biochemical studies of pooled sperm have suggested that sperm with abnormal shape may contain DNA that has not been properly repackaged by protamine during spermatid development. We have used micro-Raman spectroscopy to obtain Raman spectra from individual human sperm cells and examined how differences in the Raman spectra of sperm chromatin correlate with cell shape. We show that Raman spectra of individual sperm cells contain vibrational marker modes that can be used to assess the efficiency of DNA-packaging for each cell. Raman spectra obtained from sperm cells with normal shape provide evidence that DNA in these sperm is very efficiently packaged. We find, however, that the relative protein content per cell and DNA packaging efficiencies are distributed over a relatively wide range for sperm cells with both normal and abnormal shape. These findings indicate that single cell Raman spectroscopy should be a valuable tool in assessing the quality of sperm cells for in-vitro fertilization. (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Stichworte
sperm chromatin; packaging; DNA; cell shape abnormalities; Raman spectroscopy; human sperm cells
Erscheinungsjahr
2009
Zeitschriftentitel
Journal of Biophotonics
Band
2
Ausgabe
5
Seite(n)
322-332
ISSN
1864-063X
eISSN
1864-0648
Page URI
https://pub.uni-bielefeld.de/record/2330474

Zitieren

Huser T, Orme CA, Hollars CW, Corzett MH, Balhorn R. Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. Journal of Biophotonics. 2009;2(5):322-332.
Huser, T., Orme, C. A., Hollars, C. W., Corzett, M. H., & Balhorn, R. (2009). Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. Journal of Biophotonics, 2(5), 322-332. doi:10.1002/jbio.200910012
Huser, T., Orme, C. A., Hollars, C. W., Corzett, M. H., and Balhorn, R. (2009). Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. Journal of Biophotonics 2, 322-332.
Huser, T., et al., 2009. Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. Journal of Biophotonics, 2(5), p 322-332.
T. Huser, et al., “Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells”, Journal of Biophotonics, vol. 2, 2009, pp. 322-332.
Huser, T., Orme, C.A., Hollars, C.W., Corzett, M.H., Balhorn, R.: Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. Journal of Biophotonics. 2, 322-332 (2009).
Huser, Thomas, Orme, Christine A., Hollars, Christopher W., Corzett, Michele H., and Balhorn, Rod. “Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells”. Journal of Biophotonics 2.5 (2009): 322-332.

40 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

SERS Probing of Proteins in Gold Nanoparticle Agglomerates.
Szekeres GP, Kneipp J., Front Chem 7(), 2019
PMID: 30766868
Raman micro-spectroscopy analysis of different sperm regions: a species comparison.
Amaral S, Da Costa R, Wübbeling F, Redmann K, Schlatt S., Mol Hum Reprod 24(4), 2018
PMID: 29528451
Microfluidic-based sperm sorting & analysis for treatment of male infertility.
Samuel R, Feng H, Jafek A, Despain D, Jenkins T, Gale B., Transl Androl Urol 7(suppl 3), 2018
PMID: 30159240
Array of disordered silicon nanowires coated by a gold film for combined NIR photothermal treatment of cancer cells and Raman monitoring of the process evolution.
Convertino A, Mussi V, Maiolo L, Ledda M, Lolli MG, Bovino FA, Fortunato G, Rocchia M, Lisi A., Nanotechnology 29(41), 2018
PMID: 30059014
1H Magnetic Resonance Spectroscopy of live human sperm.
Reynolds S, Calvert SJ, Paley MN, Pacey AA., Mol Hum Reprod 23(7), 2017
PMID: 28431025
Non-Invasive Approaches to Epigenetic-Based Sperm Selection.
Štiavnická M, Abril-Parreño L, Nevoral J, Králíčková M, García-Álvarez O., Med Sci Monit 23(), 2017
PMID: 28961228
Fourier transform infrared spectroscopic analysis of sperm chromatin structure and DNA stability.
Oldenhof H, Schütze S, Wolkers WF, Sieme H., Andrology 4(3), 2016
PMID: 26916383
Structural Changes Induced in Grapevine (Vitis vinifera L.) DNA by Femtosecond IR Laser Pulses: A Surface-Enhanced Raman Spectroscopic Study.
Dina NE, Muntean CM, Leopold N, Fălămaș A, Halmagyi A, Coste A., Nanomaterials (Basel) 6(6), 2016
PMID: 28335224
Non-label immune cell state prediction using Raman spectroscopy.
Ichimura T, Chiu LD, Fujita K, Machiyama H, Yamaguchi T, Watanabe TM, Fujita H., Sci Rep 6(), 2016
PMID: 27876845
Raman microscopy for cellular investigations--From single cell imaging to drug carrier uptake visualization.
Kann B, Offerhaus HL, Windbergs M, Otto C., Adv Drug Deliv Rev 89(), 2015
PMID: 25728764
Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation.
Muntean CM, Leopold N, Tripon C, Coste A, Halmagyi A., Spectrochim Acta A Mol Biomol Spectrosc 144(), 2015
PMID: 25748988
Advantages and limitations of Raman spectroscopy for molecular diagnostics: an update.
Eberhardt K, Stiebing C, Matthäus C, Schmitt M, Popp J., Expert Rev Mol Diagn 15(6), 2015
PMID: 25872466
Raman spectroscopy for physiological investigations of tissues and cells.
Huser T, Chan J., Adv Drug Deliv Rev 89(), 2015
PMID: 26144996
Sperm selection in natural conception: what can we learn from Mother Nature to improve assisted reproduction outcomes?
Sakkas D, Ramalingam M, Garrido N, Barratt CL., Hum Reprod Update 21(6), 2015
PMID: 26386468
Rapid and label-free identification of normal spermatozoa based on image analysis and micro-Raman spectroscopy.
Huang Z, Chen G, Chen X, Wang J, Chen J, Lu P, Chen R., J Biophotonics 7(9), 2014
PMID: 23666810
Raman microspectroscopy: shining a new light on reproductive medicine.
Mallidis C, Sanchez V, Wistuba J, Wuebbeling F, Burger M, Fallnich C, Schlatt S., Hum Reprod Update 20(3), 2014
PMID: 24144514
Antioxidative protective effect of icariin on the FeSO4/H 2O 2-damaged human sperm based on confocal raman micro-spectroscopy.
Huang ZS, Xiao HJ, Qi T, Hu ZM, Li H, Chen DL, Xu YL, Chen J., J Huazhong Univ Sci Technolog Med Sci 34(5), 2014
PMID: 25318889
Real-time Raman microspectroscopy scanning of the single live sperm bound to human zona pellucida.
Liu F, Zhu Y, Liu Y, Wang X, Ping P, Zhu X, Hu H, Li Z, He L., Fertil Steril 99(3), 2013
PMID: 23148927
A novel sorting technology allows for highly efficient selection of sperm without chromatin damage.
Funaro MG, Kim HH, Mazel S, Bolyakov A, Goldstein M, Schlegel PN, Paduch DA., Syst Biol Reprod Med 59(3), 2013
PMID: 23560816
Raman spectroscopy-based approach to detect aging-related oxidative damage in the mouse oocyte.
Bogliolo L, Murrone O, Di Emidio G, Piccinini M, Ariu F, Ledda S, Tatone C., J Assist Reprod Genet 30(7), 2013
PMID: 23860776
The role of epigenetics in spermatogenesis.
Güneş S, Kulaç T., Turk J Urol 39(3), 2013
PMID: 26328105
Structural and functional integrity of spermatozoa is compromised as a consequence of acute uropathogenic E. coli-associated epididymitis.
Lang T, Dechant M, Sanchez V, Wistuba J, Boiani M, Pilatz A, Stammler A, Middendorff R, Schuler G, Bhushan S, Tchatalbachev S, Wübbeling F, Burger M, Chakraborty T, Mallidis C, Meinhardt A., Biol Reprod 89(3), 2013
PMID: 23843239
Semen analysis: update on clinical value, current needs and future perspectives.
Sánchez V, Wistuba J, Mallidis C., Reproduction 146(6), 2013
PMID: 24062567
DNA content of Ovis musimon spermatozoa.
Ferrari MR, Spirito SE, Giuliano SM, Cisale HO., Andrologia 44 Suppl 1(), 2012
PMID: 21592176
Discrimination of gastric cancer from normal by serum RNA based on surface-enhanced Raman spectroscopy (SERS) and multivariate analysis.
Chen Y, Chen G, Zheng X, He C, Feng S, Chen Y, Lin X, Chen R, Zeng H., Med Phys 39(9), 2012
PMID: 22957632
Diagnostic tools in male infertility-the question of sperm dysfunction.
Barratt CL, Mansell S, Beaton C, Tardif S, Oxenham SK., Asian J Androl 13(1), 2011
PMID: 21102478
In situ visualization of damaged DNA in human sperm by Raman microspectroscopy.
Mallidis C, Wistuba J, Bleisteiner B, Damm OS, Gross P, Wübbeling F, Fallnich C, Burger M, Schlatt S., Hum Reprod 26(7), 2011
PMID: 21531992
Raman microspectroscopy detects epigenetic modifications in living Jurkat leukemic cells.
Poplineau M, Trussardi-Régnier A, Happillon T, Dufer J, Manfait M, Bernard P, Piot O, Antonicelli F., Epigenomics 3(6), 2011
PMID: 22126296
Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa.
Meister K, Schmidt DA, Bründermann E, Havenith M., Analyst 135(6), 2010
PMID: 20386810
Evaluation of the potential of Raman microspectroscopy for prediction of chemotherapeutic response to cisplatin in lung adenocarcinoma.
Nawaz H, Bonnier F, Knief P, Howe O, Lyng FM, Meade AD, Byrne HJ., Analyst 135(12), 2010
PMID: 20931112
Physico-chemical characterization of polylipid nanoparticles for gene delivery to the liver.
Nyunt MT, Dicus CW, Cui YY, Yappert MC, Huser TR, Nantz MH, Wu J., Bioconjug Chem 20(11), 2009
PMID: 19860429

48 References

Daten bereitgestellt von Europe PubMed Central.

Chromatin structure and dynamics: Functional implications
Morales, Biochimie 83(11/12), 2001
Nucleosomes and the chromatin fiber.
Hayes JJ, Hansen JC., Curr. Opin. Genet. Dev. 11(2), 2001
PMID: 11250133
Histone synthesis and replacement during spermatogenesis in the mouse.
Goldberg RB, Geremia R, Bruce WR., Differentiation 7(3), 1977
PMID: 323095

AUTHOR UNKNOWN, 0
Packaging paternal chromosomes with protamine.
Braun RE., Nat. Genet. 28(1), 2001
PMID: 11326265
Analysis of Synthetic Dnas and DNA-Protamine Complexes with the Scanning Tunneling Microscope
Allen, Scanning Microscopy V7(N2), 1993

AUTHOR UNKNOWN, 0
Complete selective absence of protamine P2 in humans.
de Yebra L, Ballesca JL, Vanrell JA, Bassas L, Oliva R., J. Biol. Chem. 268(14), 1993
PMID: 8486708
Aberrant protamine 1/protamine 2 ratios in sperm of infertile human males.
Balhorn R, Reed S, Tanphaichitr N., Experientia 44(1), 1988
PMID: 3350120
Anomalous distribution of nuclear basic proteins in round-headed human spermatozoa.
Blanchard Y, Lescoat D, Le Lannou D., Andrologia 22(6), 1990
PMID: 2099675
Human male infertility may be due to a decrease of the protamine P2 content in sperm chromatin.
Belokopytova IA, Kostyleva EI, Tomilin AN, Vorob'ev VI., Mol. Reprod. Dev. 34(1), 1993
PMID: 8418817
Studying Single Living Cells and Chromosomes by Confocal Raman Microspectroscopy
Puppels, Nature V347(N6290), 1990
Raman spectroscopy of DNA and proteins.
Peticolas WL., Meth. Enzymol. 246(), 1995
PMID: 7538626
Conformation of DNA in vitro and in vivo from laser Raman scattering.
Peticolas WL, Evertsz E., Meth. Enzymol. 211(), 1992
PMID: 1406314

AUTHOR UNKNOWN, 0
Raman spectra of the model B-DNA oligomer d(CGCGAATTCGCG)2 and of the DNA in living salmon sperm show that both have very similar B-type conformations.
Kubasek WL, Wang Y, Thomas GA, Patapoff TW, Schoenwaelder KH, Van der Sande JH, Peticolas WL., Biochemistry 25(23), 1986
PMID: 3801423
Raman spectroscopy and microscopy of individual cells and cellular components
Chan, Laser & Photon Rev. 2(5), 2008

AUTHOR UNKNOWN, 0
Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells.
Chan JW, Taylor DS, Zwerdling T, Lane SM, Ihara K, Huser T., Biophys. J. 90(2), 2005
PMID: 16239327
Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy.
Chan JW, Esposito AP, Talley CE, Hollars CW, Lane SM, Huser T., Anal. Chem. 76(3), 2004
PMID: 14750852
Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering
Zumbusch, Physical Review Letters 82(20), 1999
Femtosecond stimulated Raman microscopy
Ploetz, Appl. Phys. B 87(), 2007
Perspective - Living cells as test tubes
Xie, Science 312(), 5771
Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy.
Freudiger CW, Min W, Saar BG, Lu S, Holtom GR, He C, Tsai JC, Kang JX, Xie XS., Science 322(5909), 2008
PMID: 19095943
Measuring DNA to Protein Ratios in Mammalian Sperm Head by Xanes Imaging
Zhang, Journal of Structural Biology V116(N3), 1996
DNA and Total Protamine Masses in Individual Sperm from Fertile Mammalian Subjects
Bench, Cytometry V23(N4), 1996
Zinc is sufficiently abundant within mammalian sperm nuclei to bind stoichiometrically with protamine 2
Bench, Molecular Reproduction and Development V56(N4), 2000
Atomic force microscope analysis of chromatin volumes in human sperm with head-shape abnormalities
Lee, Biology of Reproduction V56(N1), 1997
Quantification and classification of human sperm morphology by computer-assisted image analysis.
Moruzzi JF, Wyrobek AJ, Mayall BH, Gledhill BL., Fertil. Steril. 50(1), 1988
PMID: 3384107
Laser irradiation and Raman spectroscopy of single living cells and chromosomes: Sample degradation occurs with 514.5 nm but not with 660 nm laser light
Puppels, Exp. Cell Research 195(2), 1991
Atomic Force Microscopy of Mammalian Sperm Chromatin
Allen, Chromosoma V102(N9), 1993
Evidence of Novel Secondary Structure in DNA-Bound Protamine Is Revealed by Raman Spectroscopy
Hud, Biochemistry V33(N24), 1994
Dependence of the raman signature of genomic B-DNA on nucleotide base sequence
Deng, Biopolymers V50(N6), 1999
Melting and premelting phenomenon in DNA by laser Raman scattering.
Erfurth SC, Peticolas WL., Biopolymers 14(2), 1975
PMID: 1174661
Structural basis of polyamine-DNA recognition: spermidine and spermine interactions with genomic B-DNAs of different GC content probed by Raman spectroscopy
Deng, Nucleic Acids Research V28(N17), 2000

AUTHOR UNKNOWN, 0
On round-headed human spermatozoa.
Kullander S, Rausing A., Int. J. Fertil. 20(1), 1975
PMID: 4383
Sperm morphologic features as a prognostic factor in in vitro fertilization.
Kruger TF, Menkveld R, Stander FS, Lombard CJ, Van der Merwe JP, van Zyl JA, Smith K., Fertil. Steril. 46(6), 1986
PMID: 2946611
Zona drilling and sperm insertion with combined laser microbeam and optical tweezers.
Schutze K, Clement-Sengewald A, Ashkin A., Fertil. Steril. 61(4), 1994
PMID: 8150126

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 19373853
PubMed | Europe PMC

Suchen in

Google Scholar