Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1

McNerney GP, Hübner W, Chen BK, Huser T (2010)
Journal of Biophotonics 3(4): 216-223.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
McNerney, Gregory P.; Hübner, WolfgangUniBi ; Chen, Benjamin K.; Huser, ThomasUniBi
Abstract / Bemerkung
Cell-cell interactions through direct contact are very important for cellular communication and coordination especially for immune cells. The human immunodeficiency virus type I (HIV-1) induces immune cell interactions between CD4(+) cells to shuttle between T cells via a virological synapse. A goal to understand the process of cell-cell transmission through virological synapses is to determine the cellular states that allow a chance encounter between cells to become a stable cell-cell adhesion. We demonstrate the use of optical tweezers to manipulate uninfected primary CD4(+) T cells near HIV Gag-iGFP transfected Jurkat T cells to probe the determinants that induce stable adhesion. When combined with fast 4D confocal fluorescence microscopy, optical tweezers can be utilized not only to facilitate cell-cell contact, but also to simultaneously track the formation of a virological synapse, and ultimately to probe the events that precede virus transfer. [GRAPHICS] HIV-1 infected T cell (green) decorated with uninfected primary T cells (red) by manipulating the primary cells with an optical tweezers system.
Stichworte
fluorescence microscopy; cell-cell transmission; HIV-1; optical tweezers; micromanipulation
Erscheinungsjahr
2010
Zeitschriftentitel
Journal of Biophotonics
Band
3
Ausgabe
4
Seite(n)
216-223
ISSN
1864-063X
eISSN
1864-0648
Page URI
https://pub.uni-bielefeld.de/record/2330468

Zitieren

McNerney GP, Hübner W, Chen BK, Huser T. Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics. 2010;3(4):216-223.
McNerney, G. P., Hübner, W., Chen, B. K., & Huser, T. (2010). Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics, 3(4), 216-223. https://doi.org/10.1002/jbio.200900102
McNerney, Gregory P., Hübner, Wolfgang, Chen, Benjamin K., and Huser, Thomas. 2010. “Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”. Journal of Biophotonics 3 (4): 216-223.
McNerney, G. P., Hübner, W., Chen, B. K., and Huser, T. (2010). Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics 3, 216-223.
McNerney, G.P., et al., 2010. Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics, 3(4), p 216-223.
G.P. McNerney, et al., “Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”, Journal of Biophotonics, vol. 3, 2010, pp. 216-223.
McNerney, G.P., Hübner, W., Chen, B.K., Huser, T.: Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1. Journal of Biophotonics. 3, 216-223 (2010).
McNerney, Gregory P., Hübner, Wolfgang, Chen, Benjamin K., and Huser, Thomas. “Manipulating CD4(+) T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”. Journal of Biophotonics 3.4 (2010): 216-223.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Rapid 3D fluorescence imaging of individual optically trapped living immune cells.
Wolfson D, Steck M, Persson M, McNerney G, Popovich A, Goksör M, Huser T., J Biophotonics 8(3), 2015
PMID: 24420444
Mechanisms of enhanced HIV spread through T-cell virological synapses.
Dale BM, Alvarez RA, Chen BK., Immunol Rev 251(1), 2013
PMID: 23278744
Optically trapped microsensors for microfluidic temperature measurement by fluorescence lifetime imaging microscopy.
Bennet MA, Richardson PR, Arlt J, McCarthy A, Buller GS, Jones AC., Lab Chip 11(22), 2011
PMID: 21956603

38 References

Daten bereitgestellt von Europe PubMed Central.

Notch signals control the fate of immature progenitor cells in the intestine.
Fre S, Huyghe M, Mourikis P, Robine S, Louvard D, Artavanis-Tsakonas S., Nature 435(7044), 2005
PMID: 15959516
The tight junction: a multifunctional complex.
Schneeberger EE, Lynch RD., Am. J. Physiol., Cell Physiol. 286(6), 2004
PMID: 15151915
The gap junction communication channel.
Kumar NM, Gilula NB., Cell 84(3), 1996
PMID: 8608591
Cell biology of the presynaptic terminal.
Murthy VN, De Camilli P., Annu. Rev. Neurosci. 26(), 2003
PMID: 14527272
The synaptic vesicle cycle.
Sudhof TC., Annu. Rev. Neurosci. 27(), 2004
PMID: 15217342
Dynamic signaling between astrocytes and neurons.
Araque A, Carmignoto G, Haydon PG., Annu. Rev. Physiol. 63(), 2001
PMID: 11181976

Çakur T, Alsan S, Saybaşulu H, Akun A., 2007
Glia-neuron intercommunications and synaptic plasticity.
Vernadakis A., Prog. Neurobiol. 49(3), 1996
PMID: 8878303

Erickson J, Tooker A, Tai Y-C, Pine J., 2008
Laser stimulation of auditory neurons: effect of shorter pulse duration and penetration depth.
Izzo AD, Walsh JT Jr, Ralph H, Webb J, Bendett M, Wells J, Richter CP., Biophys. J. 94(8), 2008
PMID: 18192375
CD1: antigen presentation and T cell function.
Brigl M, Brenner MB., Annu. Rev. Immunol. 22(), 2004
PMID: 15032598
The immunological synapse.
Bromley SK, Burack WR, Johnson KG, Somersalo K, Sims TN, Sumen C, Davis MM, Shaw AS, Allen PM, Dustin ML., Annu. Rev. Immunol. 19(), 2001
PMID: 11244041
The immunological synapse: a molecular machine controlling T cell activation.
Grakoui A, Bromley SK, Sumen C, Davis MM, Shaw AS, Allen PM, Dustin ML., Science 285(5425), 1999
PMID: 10398592
Antigen presentation in extracellular matrix: interactions of T cells with dendritic cells are dynamic, short lived, and sequential.
Gunzer M, Schafer A, Borgmann S, Grabbe S, Zanker KS, Brocker EB, Kampgen E, Friedl P., Immunity 13(3), 2000
PMID: 11021530
Green fluorescent protein as a marker for gene expression.
Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC., Science 263(5148), 1994
PMID: 8303295
Creating new fluorescent probes for cell biology.
Zhang J, Campbell RE, Ting AY, Tsien RY., Nat. Rev. Mol. Cell Biol. 3(12), 2002
PMID: 12461557
Studying protein dynamics in living cells.
Lippincott-Schwartz J, Snapp E, Kenworthy A., Nat. Rev. Mol. Cell Biol. 2(6), 2001
PMID: 11389468
Understanding, improving and using green fluorescent proteins.
Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY., Trends Biochem. Sci. 20(11), 1995
PMID: 8578587
The fluorescent toolbox for assessing protein location and function.
Giepmans BN, Adams SR, Ellisman MH, Tsien RY., Science 312(5771), 2006
PMID: 16614209
Quantitative 3D video microscopy of HIV transfer across T cell virological synapses.
Hubner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FY, Li XD, Asmuth DM, Huser T, Chen BK., Science 323(5922), 2009
PMID: 19325119
HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse.
Jolly C, Kashefi K, Hollinshead M, Sattentau QJ., J. Exp. Med. 199(2), 2004
PMID: 14734528
Retroviral spread by induction of virological synapses.
Jolly C, Sattentau QJ., Traffic 5(9), 2004
PMID: 15296489
Modulation of the immunological synapse: a key to HIV-1 pathogenesis?
Fackler OT, Alcover A, Schwartz O., Nat. Rev. Immunol. 7(4), 2007
PMID: 17380160

Ashkin A., 1970
Observation of a single-beam gradient force optical trap for dielectric particles.
Ashkin A, Dziedzic JM, Bjorkholm JE, Chu S., Opt Lett 11(5), 1986
PMID: 19730608
Nondestructive identification of individual leukemia cells by laser trapping Raman spectroscopy.
Chan JW, Taylor DS, Lane SM, Zwerdling T, Tuscano J, Huser T., Anal. Chem. 80(6), 2008
PMID: 18260656

Ozkan M, Wang M, Ozkan C, Flynn R, Birkbeck A, Esener S., 2003
Review of cell and particle trapping in microfluidic systems.
Nilsson J, Evander M, Hammarstrom B, Laurell T., Anal. Chim. Acta 649(2), 2009
PMID: 19699390
Raman study of mechanically induced oxygenation state transition of red blood cells using optical tweezers.
Rao S, Balint S, Cossins B, Guallar V, Petrov D., Biophys. J. 96(1), 2009
PMID: 18931252

Shi L, Shao B, Chen T, Berns M., 2009
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