Single Cell Analysis in Full Body Quartz Glass Chips with Native UV-Laser Induced Fluorescence Detection

Greif D, Galla L, Ros A, Anselmetti D (2008)
Journal of Chromatography A 1206(1): 83-88.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Abstract / Bemerkung
In order to investigate the individual and inhomogenous Cellular response, e.g. to external Stimuli, single cell analysis is mandatory and may provide new cognitions in proteomics as well as in other fields of systems biology in the future. Here, we report on novel chip architectures for single cell analysis based on full body quartz glass microfluidic chips (QG chips) that extend our previous studies in polydimethylsiloxane (PDMS) chips, and enhance the detection sensitivity of native UV laser-induced fluorescence (UV-LIF) detection. Detection of a 10 nM tryptophan Solution With ail S/N ratio of 11.9. which gives a theoretical limit of detection of 2.5 nM (with S/N=3). was possible. With these optimizations the three proteins alpha-chymotrypsinogen A, ovalbumin and catalase each at a concentration of 100 mu g/mL (equal to 4 mu M, 0.4 mu M and 2.2 mu M) were injected electrokinetically and could be separated with nearly baseline resolution. Furthermore, fluorescence spectra (excitation wavelength, lambda(ex) = 266 nm) clearly demonstrate the favourable properties like the very high UV transparency and the nearly vanishing background fluorescence of the QG chips as compared to PDMS chips and to PDMS quartz window (PQW) chips. Finally we exploit the improved sensitivity for single cell electropherograms of Spodoptera frugiperda (Sf9) insect cells. (C) 2008 Elsevier B.V. All rights reserved.
Stichworte
Dry etching; Quartz; Single cell analysis; Protein; Label-free UV-LIF detection; microfluidic chip
Erscheinungsjahr
2008
Zeitschriftentitel
Journal of Chromatography A
Band
1206
Ausgabe
1
Seite(n)
83-88
ISSN
0021-9673
Page URI
https://pub.uni-bielefeld.de/record/2310968

Zitieren

Greif D, Galla L, Ros A, Anselmetti D. Single Cell Analysis in Full Body Quartz Glass Chips with Native UV-Laser Induced Fluorescence Detection. Journal of Chromatography A. 2008;1206(1):83-88.
Greif, D., Galla, L., Ros, A., & Anselmetti, D. (2008). Single Cell Analysis in Full Body Quartz Glass Chips with Native UV-Laser Induced Fluorescence Detection. Journal of Chromatography A, 1206(1), 83-88. doi:10.1016/j.chroma.2008.07.013
Greif, D., Galla, L., Ros, A., and Anselmetti, D. (2008). Single Cell Analysis in Full Body Quartz Glass Chips with Native UV-Laser Induced Fluorescence Detection. Journal of Chromatography A 1206, 83-88.
Greif, D., et al., 2008. Single Cell Analysis in Full Body Quartz Glass Chips with Native UV-Laser Induced Fluorescence Detection. Journal of Chromatography A, 1206(1), p 83-88.
D. Greif, et al., “Single Cell Analysis in Full Body Quartz Glass Chips with Native UV-Laser Induced Fluorescence Detection”, Journal of Chromatography A, vol. 1206, 2008, pp. 83-88.
Greif, D., Galla, L., Ros, A., Anselmetti, D.: Single Cell Analysis in Full Body Quartz Glass Chips with Native UV-Laser Induced Fluorescence Detection. Journal of Chromatography A. 1206, 83-88 (2008).
Greif, Dominik, Galla, Lukas, Ros, Alexandra, and Anselmetti, Dario. “Single Cell Analysis in Full Body Quartz Glass Chips with Native UV-Laser Induced Fluorescence Detection”. Journal of Chromatography A 1206.1 (2008): 83-88.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 18657818
PubMed | Europe PMC

Suchen in

Google Scholar