Liposomes alter thermal phase behavior and composition of red blood cell membranes

Stoll C, Stadnick H, Kollas O, Holovati JL, Glasmacher B, Acker JP, Wolkers WF (2011)
Biochimica et Biophysica Acta (BBA) - Biomembranes 1808(1): 474-481.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Autor/in
Stoll, Christoph; Stadnick, Hart; Kollas, OliverUniBi; Holovati, Jelena L.; Glasmacher, Birgit; Acker, Jason P.; Wolkers, Willem F.
Abstract / Bemerkung
Unilamellar liposomes composed of natural phospholipids provide a new promising class of protective agents for hypothermic storage, cryopreservation, or freeze-drying of red blood cells (RBCs). In this study, FTIR spectroscopy, MALDI-TOF MS, and colorimetric assays were used to investigate the effects of liposomes composed of a homologous series of linear saturated phosphatidylcholine phospholipids (18:0; 16:0; 14:0; 12:0) on RBC membranes. RBCs were incubated with liposomes at 37 degrees C and both the liposomal and the RBC fraction were analyzed after incubation. FTIR studies showed that liposomes composed of short acyl chain length lipids cause an increase in RBC membrane conformational disorder at suprazero temperatures, whereas long acyl chain length lipids were found to have little effects. The increased lipid conformational disorder in the RBC membranes coincided with a decrease in the cholesterol-to-phospholipid ratio. The opposite effects were found in the liposomes after incubation with RBCs. MALDI-TOF MS analysis showed the presence of short acyl chain length lipids (14:0 and 12:0) in RBC membranes after incubation, which was not observed after incubation with liposomes containing long acyl chain length lipids (18:0 and 16:0). Liposomes alter RBC membrane properties by cholesterol depletion and lipid addition. (C) 2010 Elsevier B.V. All rights reserved.
Stichworte
Lipid phase behavior; Fourier transform infrared spectroscopy; Liposome; MALDI-TOF mass spectrometry; Erythrocyte; Cholesterol transfer
Erscheinungsjahr
2011
Zeitschriftentitel
Biochimica et Biophysica Acta (BBA) - Biomembranes
Band
1808
Ausgabe
1
Seite(n)
474-481
ISSN
0005-2736
Page URI
https://pub.uni-bielefeld.de/record/2094563

Zitieren

Stoll C, Stadnick H, Kollas O, et al. Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes. 2011;1808(1):474-481.
Stoll, C., Stadnick, H., Kollas, O., Holovati, J. L., Glasmacher, B., Acker, J. P., & Wolkers, W. F. (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1808(1), 474-481. doi:10.1016/j.bbamem.2010.09.012
Stoll, C., Stadnick, H., Kollas, O., Holovati, J. L., Glasmacher, B., Acker, J. P., and Wolkers, W. F. (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes 1808, 474-481.
Stoll, C., et al., 2011. Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1808(1), p 474-481.
C. Stoll, et al., “Liposomes alter thermal phase behavior and composition of red blood cell membranes”, Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1808, 2011, pp. 474-481.
Stoll, C., Stadnick, H., Kollas, O., Holovati, J.L., Glasmacher, B., Acker, J.P., Wolkers, W.F.: Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1808, 474-481 (2011).
Stoll, Christoph, Stadnick, Hart, Kollas, Oliver, Holovati, Jelena L., Glasmacher, Birgit, Acker, Jason P., and Wolkers, Willem F. “Liposomes alter thermal phase behavior and composition of red blood cell membranes”. Biochimica et Biophysica Acta (BBA) - Biomembranes 1808.1 (2011): 474-481.

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