Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti

Voss B, Holscher M, Baumgarth B, Kalbfleisch A, Kaya C, Hess WR, Becker A, Evguenieva-Hackenberg E (2009)
Biochemical and Biophysical Research Communications 390(2): 331-336.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Voss, Björn; Holscher, Marion; Baumgarth, Birgit; Kalbfleisch, Andreas; Kaya, Ceren; Hess, Wolfgang R.; Becker, Anke; Evguenieva-Hackenberg, Elena
Einrichtung
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Abstract / Bemerkung
Regulatory RNA plays a pivotal role in the regulation of bacterial gene expression. Here, five small RNAs were studied in Sinorhizobium meliloti - SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium species. The amount of SmrC16 decreased in stationary phase, while the other RNAs were up-regulated. The smallest changes, maximally 2-fold, were observed for 6S RNA. In the distantly related Bradyrhizobium japonicum, the amount of 6S RNA was not increased in stationary phase, suggesting some functional divergence in the roles of this molecule in Rhizobiales in comparison to Escherichia coli. Different decay rates were observed for SmrC15 and SmrC16 of S. meliloti upon rifampicin treatment, revealing posttranscriptional regulation during growth. The use of a Deltahfq mutant showed that Hfq protects full-length SmrC16 from degradation and stabilises its specific degradation products.
Erscheinungsjahr
2009
Zeitschriftentitel
Biochemical and Biophysical Research Communications
Band
390
Ausgabe
2
Seite(n)
331-336
ISSN
0006-291X
Page URI
https://pub.uni-bielefeld.de/record/2092111

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Voss B, Holscher M, Baumgarth B, et al. Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti. Biochemical and Biophysical Research Communications. 2009;390(2):331-336.
Voss, B., Holscher, M., Baumgarth, B., Kalbfleisch, A., Kaya, C., Hess, W. R., Becker, A., et al. (2009). Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti. Biochemical and Biophysical Research Communications, 390(2), 331-336. https://doi.org/10.1016/j.bbrc.2009.09.125
Voss, Björn, Holscher, Marion, Baumgarth, Birgit, Kalbfleisch, Andreas, Kaya, Ceren, Hess, Wolfgang R., Becker, Anke, and Evguenieva-Hackenberg, Elena. 2009. “Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti”. Biochemical and Biophysical Research Communications 390 (2): 331-336.
Voss, B., Holscher, M., Baumgarth, B., Kalbfleisch, A., Kaya, C., Hess, W. R., Becker, A., and Evguenieva-Hackenberg, E. (2009). Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti. Biochemical and Biophysical Research Communications 390, 331-336.
Voss, B., et al., 2009. Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti. Biochemical and Biophysical Research Communications, 390(2), p 331-336.
B. Voss, et al., “Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti”, Biochemical and Biophysical Research Communications, vol. 390, 2009, pp. 331-336.
Voss, B., Holscher, M., Baumgarth, B., Kalbfleisch, A., Kaya, C., Hess, W.R., Becker, A., Evguenieva-Hackenberg, E.: Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti. Biochemical and Biophysical Research Communications. 390, 331-336 (2009).
Voss, Björn, Holscher, Marion, Baumgarth, Birgit, Kalbfleisch, Andreas, Kaya, Ceren, Hess, Wolfgang R., Becker, Anke, and Evguenieva-Hackenberg, Elena. “Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti”. Biochemical and Biophysical Research Communications 390.2 (2009): 331-336.

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