Characterization of the Medicago truncatula cell wall proteome in cell suspension culture upon elicitation and suppression of plant defense

Gokulakannan GG, Niehaus K (2010)
Journal of Plant Physiology 167(18): 1533-1541.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Gokulakannan, Gomathi Gandhi; Niehaus, KarstenUniBi
Abstract / Bemerkung
In addition to establishing methods for proteome analysis of cell wall proteins (CWPs) for the model plant Medicago truncatula, this work highlights the presence of several protein classes in cell culture. Using a combination of two-dimensional gel electrophoresis (2D-PAGE) and/or liquid chromatography-tandem mass spectrometry (LC-MS/MS), we established the proteome reference map of M. truncatula cell wall proteins. CWPs extracted from purified cell wall fragments resulted in the identification of 46 (2D-PAGE) and 65 (LC-MS/MS) proteins, respectively, with a total of 111 proteins. The identified proteins are involved in various processes, including cell wall modifications, signaling, defense mechanisms, membrane transport, protein synthesis and processing. Further, we conducted comparative proteome analysis to identify changes in protein composition during interaction of M. truncatula cell suspension culture with a pathogen-derived yeast elicitor (YE) and suppressor using Sinorhizobium meliloti LPS. 2D-PAGE analysis for the CWPs after YE and LPS treatment resembled the proteome map of YE alone, with a few up-regulated proteins involved in defense, and in the case of the LPS-treated cell wall proteome, there was no significant difference observed. Using this approach, proteins involved in defense, such as L-ascorbate peroxidase, specifically targeted proteins to the cell wall during defense, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and proteins that play an important role during growth and development were identified. Also, some defense-related proteins were absent in the same gel after YE treatment, suggesting that oxidant protection is regulated by these proteins. (C) 2010 Elsevier GmbH. All rights reserved.
Stichworte
LC-MS/MS; Cell wall proteins; Medicago truncatula; Elicitor; Suppressor
Erscheinungsjahr
2010
Zeitschriftentitel
Journal of Plant Physiology
Band
167
Ausgabe
18
Seite(n)
1533-1541
ISSN
0176-1617
Page URI
https://pub.uni-bielefeld.de/record/2003409

Zitieren

Gokulakannan GG, Niehaus K. Characterization of the Medicago truncatula cell wall proteome in cell suspension culture upon elicitation and suppression of plant defense. Journal of Plant Physiology. 2010;167(18):1533-1541.
Gokulakannan, G. G., & Niehaus, K. (2010). Characterization of the Medicago truncatula cell wall proteome in cell suspension culture upon elicitation and suppression of plant defense. Journal of Plant Physiology, 167(18), 1533-1541. doi:10.1016/j.jplph.2010.06.023
Gokulakannan, G. G., and Niehaus, K. (2010). Characterization of the Medicago truncatula cell wall proteome in cell suspension culture upon elicitation and suppression of plant defense. Journal of Plant Physiology 167, 1533-1541.
Gokulakannan, G.G., & Niehaus, K., 2010. Characterization of the Medicago truncatula cell wall proteome in cell suspension culture upon elicitation and suppression of plant defense. Journal of Plant Physiology, 167(18), p 1533-1541.
G.G. Gokulakannan and K. Niehaus, “Characterization of the Medicago truncatula cell wall proteome in cell suspension culture upon elicitation and suppression of plant defense”, Journal of Plant Physiology, vol. 167, 2010, pp. 1533-1541.
Gokulakannan, G.G., Niehaus, K.: Characterization of the Medicago truncatula cell wall proteome in cell suspension culture upon elicitation and suppression of plant defense. Journal of Plant Physiology. 167, 1533-1541 (2010).
Gokulakannan, Gomathi Gandhi, and Niehaus, Karsten. “Characterization of the Medicago truncatula cell wall proteome in cell suspension culture upon elicitation and suppression of plant defense”. Journal of Plant Physiology 167.18 (2010): 1533-1541.

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7 References

Daten bereitgestellt von Europe PubMed Central.

Plant cell wall proteomics: mass spectrometry data, a trove for research on protein structure/function relationships.
Albenne C, Canut H, Boudart G, Zhang Y, San Clemente H, Pont-Lezica R, Jamet E., Mol Plant 2(5), 2009
PMID: 19825673
Plants interact with microbial polysaccharides.
Albersheim P, Ayers AR, Valent BS, Ebel J, Hahn M, Wolpert J, Carlson R., J. Supramol. Struct. 6(4), 1977
PMID: 592826
3-phosphoglycerate kinase: a glycolytic enzyme protein present in the cell wall of Candida albicans.
Alloush HM, Lopez-Ribot JL, Masten BJ, Chaffin WL., Microbiology (Reading, Engl.) 143 ( Pt 2)(), 1997
PMID: 9043109
Arabidopsis cell wall proteome defined using multidimensional protein identification technology.
Bayer EM, Bottrill AR, Walshaw J, Vigouroux M, Naldrett MJ, Thomas CL, Maule AJ., Proteomics 6(1), 2006
PMID: 16287169
Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: a critical analysis.
Borderies G, Jamet E, Lafitte C, Rossignol M, Jauneau A, Boudart G, Monsarrat B, Esquerre-Tugaye MT, Boudet A, Pont-Lezica R., Electrophoresis 24(19-20), 2003
PMID: 14595688

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