Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethylene imine): a dynamic light scattering study

Hellweg T, Henry-Toulme N, Chambon M, Roux D (2000)
In: Colloids and Surfaces A Physicochemical and Engineering Aspects., 163(1). Elsevier: 71-80.

Konferenzbeitrag | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ;
Abstract / Bemerkung
In genetic engineering and gene therapy, it is important to obtain high transfection efficiencies. This can be achieved by condensation or aggregation of the DNA using the attractive interaction of DNA with cationic polyelectrolytes or histones. In recent years, the use of poly(ethylene imine) has gained importance in this held. In our study, we characterize the hydrodynamic properties of the dendrimeric cationic polyelectrolyte poly(ethylene imine) (PEI; M-W = 25 kDa) and of short DNA fragments (approximate to 150 bp) in solution by dynamic light scattering. On this basis we determine structural parameters of the formed aggregates. On mixing the DNA fragments with the cationic polyelectrolyte, an instantaneous formation of large aggregates is found. This is different compared to condensation with small cations, which can not condense short DNA fragments. The experimental conditions (pH, etc.) during the measurements correspond to the situation in transfection experiments. (C) 2000 Elsevier Science B.V. All rights reserved.
Stichworte
DNA condensation; dynamic light scattering; translational; diffusion; PEI; transfection
Erscheinungsjahr
2000
Titel des Konferenzbandes
Colloids and Surfaces A Physicochemical and Engineering Aspects
Band
163
Ausgabe
1
Seite(n)
71-80
ISSN
0927-7757
Page URI
https://pub.uni-bielefeld.de/record/2000271

Zitieren

Hellweg T, Henry-Toulme N, Chambon M, Roux D. Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethylene imine): a dynamic light scattering study. In: Colloids and Surfaces A Physicochemical and Engineering Aspects. Vol 163. Elsevier; 2000: 71-80.
Hellweg, T., Henry-Toulme, N., Chambon, M., & Roux, D. (2000). Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethylene imine): a dynamic light scattering study. Colloids and Surfaces A Physicochemical and Engineering Aspects, 163, 71-80. Elsevier. doi:10.1016/S0927-7757(99)00432-X
Hellweg, T., Henry-Toulme, N., Chambon, M., and Roux, D. (2000). “Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethylene imine): a dynamic light scattering study” in Colloids and Surfaces A Physicochemical and Engineering Aspects, vol. 163, (Elsevier), 71-80.
Hellweg, T., et al., 2000. Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethylene imine): a dynamic light scattering study. In Colloids and Surfaces A Physicochemical and Engineering Aspects. no.163 Elsevier, pp. 71-80.
T. Hellweg, et al., “Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethylene imine): a dynamic light scattering study”, Colloids and Surfaces A Physicochemical and Engineering Aspects, vol. 163, Elsevier, 2000, pp.71-80.
Hellweg, T., Henry-Toulme, N., Chambon, M., Roux, D.: Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethylene imine): a dynamic light scattering study. Colloids and Surfaces A Physicochemical and Engineering Aspects. 163, p. 71-80. Elsevier (2000).
Hellweg, Thomas, Henry-Toulme, N, Chambon, M, and Roux, D. “Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethylene imine): a dynamic light scattering study”. Colloids and Surfaces A Physicochemical and Engineering Aspects. Elsevier, 2000.Vol. 163. 71-80.