Arrangement and expression of integrated adenovirus type 12 DNA in the transformed hamster cell line HA12/7: amplification of Ad12 and c-myc DNAs and evidence for hybrid viral-cellular transcripts

Jessberger R, Weisshaar B, Stabel S, Doerfler W (1989)
Virus Research 13(2): 113-128.

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Autor*in
Jessberger, R; Weisshaar, BerndUniBi ; Stabel, S; Doerfler, W
Abstract / Bemerkung
In the genome of the adenovirus type 12 (Ad12)-transformed hamster cell line HA12/7 about three copies of the viral DNA are fixed by integration. The results of blot-hybridization, molecular cloning, and nucleotide sequencing experiments suggest a model for the arrangement of Ad12 DNA molecules in which the left hand terminus of one of the Ad12 DNA copies is linked to unique hamster DNA. The right hand end of this DNA molecule is fused to an inverted copy of a left terminal approximately 4.3 kb fragment of Ad12 DNA. This ensemble is followed by the second Ad12 DNA copy whose right terminus is again joined to an inverted, supernumerary left terminal approximately 4.3 kb Ad12 DNA fragment. There is a third Ad12 DNA copy whose right terminus is linked to cellular DNA. In this sequence arrangement, the left terminus of Ad12 DNA is overrepresented, as had been shown earlier (S. Stabel, W. Doerfler and R.R. Friis (1980) J. Virol. 36, 22-40). In the presented model, cellular DNA sequences are interspersed in between the three copies of Ad12 DNA. In the left terminus of the integrated Ad12 DNA, transcription of RNA is initiated which extends out into cellular DNA. The interviral DNA junctions are also transcribed. The c-myc gene in cell line HA12/7 is amplified about 10-fold and considerably more c-myc RNA has been identified in the Ad12-transformed cells than in BHK21 or in LSH hamster cells. It has been shown previously that the E1 region of Ad12 DNA is transcribed into mRNA in HA12/7 cells (Ortin et al. (1976) J. Virol. 20, 355-372). It remains to be investigated whether c-myc amplification and expression are related to the transformed phenotype of HA12/7 cells.
Erscheinungsjahr
1989
Zeitschriftentitel
Virus Research
Band
13
Ausgabe
2
Seite(n)
113-128
ISSN
0168-1702
Page URI
https://pub.uni-bielefeld.de/record/1990283

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Jessberger R, Weisshaar B, Stabel S, Doerfler W. Arrangement and expression of integrated adenovirus type 12 DNA in the transformed hamster cell line HA12/7: amplification of Ad12 and c-myc DNAs and evidence for hybrid viral-cellular transcripts. Virus Research. 1989;13(2):113-128.
Jessberger, R., Weisshaar, B., Stabel, S., & Doerfler, W. (1989). Arrangement and expression of integrated adenovirus type 12 DNA in the transformed hamster cell line HA12/7: amplification of Ad12 and c-myc DNAs and evidence for hybrid viral-cellular transcripts. Virus Research, 13(2), 113-128. https://doi.org/10.1016/0168-1702(89)90010-5
Jessberger, R., Weisshaar, B., Stabel, S., and Doerfler, W. (1989). Arrangement and expression of integrated adenovirus type 12 DNA in the transformed hamster cell line HA12/7: amplification of Ad12 and c-myc DNAs and evidence for hybrid viral-cellular transcripts. Virus Research 13, 113-128.
Jessberger, R., et al., 1989. Arrangement and expression of integrated adenovirus type 12 DNA in the transformed hamster cell line HA12/7: amplification of Ad12 and c-myc DNAs and evidence for hybrid viral-cellular transcripts. Virus Research, 13(2), p 113-128.
R. Jessberger, et al., “Arrangement and expression of integrated adenovirus type 12 DNA in the transformed hamster cell line HA12/7: amplification of Ad12 and c-myc DNAs and evidence for hybrid viral-cellular transcripts”, Virus Research, vol. 13, 1989, pp. 113-128.
Jessberger, R., Weisshaar, B., Stabel, S., Doerfler, W.: Arrangement and expression of integrated adenovirus type 12 DNA in the transformed hamster cell line HA12/7: amplification of Ad12 and c-myc DNAs and evidence for hybrid viral-cellular transcripts. Virus Research. 13, 113-128 (1989).
Jessberger, R, Weisshaar, Bernd, Stabel, S, and Doerfler, W. “Arrangement and expression of integrated adenovirus type 12 DNA in the transformed hamster cell line HA12/7: amplification of Ad12 and c-myc DNAs and evidence for hybrid viral-cellular transcripts”. Virus Research 13.2 (1989): 113-128.

7 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

The structure of adenovirus type 12 DNA integration sites in the hamster cell genome.
Knoblauch M, Schröer J, Schmitz B, Doerfler W., J Virol 70(6), 1996
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The initiation of de novo methylation of foreign DNA integrated into a mammalian genome is not exclusively targeted by nucleotide sequence.
Orend G, Knoblauch M, Kämmer C, Tjia ST, Schmitz B, Linkwitz A, Meyer G, Maas J, Doerfler W., J Virol 69(2), 1995
PMID: 7815498
Is persistent adenovirus 12 infection involved in coeliac disease? A search for viral DNA using the polymerase chain reaction.
Mahon J, Blair GE, Wood GM, Scott BB, Losowsky MS, Howdle PD., Gut 32(10), 1991
PMID: 1955164

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