The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging

van de Linde S, Wolter S, Heilemann M, Sauer M (2010)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Abstract / Bemerkung
Super-resolution fluorescence imaging methods based on reversible photoswitching of fluorophores with subsequent localization currently develop to promising tools for cellular imaging. Since most of these methods rely on the transfer of the majority of fluorophores to a non-fluorescent dark state and precise localization of separated fluorescent fluorophores, the photophysical properties of photoswitchable fluorophores have to be carefully controlled. The achievable resolution and herewith the ability to resolve a structural feature depends not only on the brightness of the fluorophores, but also on the labeling density and on the stability or lifetime of the non-fluorescent dark state. Here, we discuss how the ratio of off- and on-switching of a fluorophore affects resolution. We compare experimental data with theoretical simulations and present a strategy to customize photoswitching characteristics to achieve optimal optical resolution. (C) 2010 Elsevier B.V. All rights reserved.
Diffraction limit; Single-molecule based localization microscopy; Photophysics; Molecular photoswitches; Super-resolution imaging; dSTORM
Page URI


van de Linde S, Wolter S, Heilemann M, Sauer M. The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. JOURNAL OF BIOTECHNOLOGY. 2010;149(4):260-266.
van de Linde, S., Wolter, S., Heilemann, M., & Sauer, M. (2010). The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. JOURNAL OF BIOTECHNOLOGY, 149(4), 260-266.
van de Linde, S., Wolter, S., Heilemann, M., and Sauer, M. (2010). The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. JOURNAL OF BIOTECHNOLOGY 149, 260-266.
van de Linde, S., et al., 2010. The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. JOURNAL OF BIOTECHNOLOGY, 149(4), p 260-266.
S. van de Linde, et al., “The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging”, JOURNAL OF BIOTECHNOLOGY, vol. 149, 2010, pp. 260-266.
van de Linde, S., Wolter, S., Heilemann, M., Sauer, M.: The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. JOURNAL OF BIOTECHNOLOGY. 149, 260-266 (2010).
van de Linde, Sebastian, Wolter, Steve, Heilemann, Mike, and Sauer, Markus. “The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging”. JOURNAL OF BIOTECHNOLOGY 149.4 (2010): 260-266.

49 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

mmSTORM: Multimodal localization based super-resolution microscopy.
Gajdos T, Cserteg Z, Szikora S, Novák T, H Kovács BB, Szabó G, Mihály J, Erdélyi M., Sci Rep 9(1), 2019
PMID: 30692575
Resolution limit of image analysis algorithms.
Cohen EAK, Abraham AV, Ramakrishnan S, Ober RJ., Nat Commun 10(1), 2019
PMID: 30770826
Photocatalysis and self-catalyzed photobleaching with covalently-linked chromophore-quencher conjugates built around BOPHY.
Sirbu D, Woodford OJ, Benniston AC, Harriman A., Photochem Photobiol Sci 17(6), 2018
PMID: 29717745
Template-free 2D particle fusion in localization microscopy.
Heydarian H, Schueder F, Strauss MT, van Werkhoven B, Fazel M, Lidke KA, Jungmann R, Stallinga S, Rieger B., Nat Methods 15(10), 2018
PMID: 30224671
A Platform To Enhance Quantitative Single Molecule Localization Microscopy.
Golfetto O, Wakefield DL, Cacao EE, Avery KN, Kenyon V, Jorand R, Tobin SJ, Biswas S, Gutierrez J, Clinton R, Ma Y, Horne DA, Williams JC, Jovanović-Talisman T., J Am Chem Soc 140(40), 2018
PMID: 30256630
Superresolution microscopy with novel BODIPY-based fluorophores.
Bittel AM, Saldivar IS, Dolman NJ, Nan X, Gibbs SL., PLoS One 13(10), 2018
PMID: 30366346
Navigating challenges in the application of superresolution microscopy.
Lambert TJ, Waters JC., J Cell Biol 216(1), 2017
PMID: 27920217
Local dimensionality determines imaging speed in localization microscopy.
Fox-Roberts P, Marsh R, Pfisterer K, Jayo A, Parsons M, Cox S., Nat Commun 8(), 2017
PMID: 28079054
Unraveling the Thousand Word Picture: An Introduction to Super-Resolution Data Analysis.
Lee A, Tsekouras K, Calderon C, Bustamante C, Pressé S., Chem Rev 117(11), 2017
PMID: 28414216
TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy.
Novák T, Gajdos T, Sinkó J, Szabó G, Erdélyi M., Sci Rep 7(1), 2017
PMID: 28424492
CRISPR-Cas9 Mediated Labelling Allows for Single Molecule Imaging and Resolution.
Khan AO, Simms VA, Pike JA, Thomas SG, Morgan NV., Sci Rep 7(1), 2017
PMID: 28814796
Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy.
Nahidiazar L, Agronskaia AV, Broertjes J, van den Broek B, Jalink K., PLoS One 11(7), 2016
PMID: 27391487
Methodology for Quantitative Characterization of Fluorophore Photoswitching to Predict Superresolution Microscopy Image Quality.
Bittel AM, Nickerson A, Saldivar IS, Dolman NJ, Nan X, Gibbs SL., Sci Rep 6(), 2016
PMID: 27412307
PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins.
Leyton-Puig D, Kedziora KM, Isogai T, van den Broek B, Jalink K, Innocenti M., Biol Open 5(7), 2016
PMID: 27378434
Super-Resolution Imaging of Plasma Membrane Proteins with Click Chemistry.
Mateos-Gil P, Letschert S, Doose S, Sauer M., Front Cell Dev Biol 4(), 2016
PMID: 27668214
Diffraction-unlimited imaging: from pretty pictures to hard numbers.
Vandenberg W, Leutenegger M, Lasser T, Hofkens J, Dedecker P., Cell Tissue Res 360(1), 2015
PMID: 25722085
Resolution improvement by 3D particle averaging in localization microscopy.
Broeken J, Johnson H, Lidke DS, Liu S, Nieuwenhuizen RP, Stallinga S, Lidke KA, Rieger B., Methods Appl Fluoresc 3(1), 2015
PMID: 25866640
Quantifying and optimizing single-molecule switching nanoscopy at high speeds.
Lin Y, Long JJ, Huang F, Duim WC, Kirschbaum S, Zhang Y, Schroeder LK, Rebane AA, Velasco MG, Virrueta A, Moonan DW, Jiao J, Hernandez SY, Zhang Y, Bewersdorf J., PLoS One 10(5), 2015
PMID: 26011109
Artifacts in single-molecule localization microscopy.
Burgert A, Letschert S, Doose S, Sauer M., Histochem Cell Biol 144(2), 2015
PMID: 26138928
Photophysical processes in single molecule organic fluorescent probes.
Stennett EM, Ciuba MA, Levitus M., Chem Soc Rev 43(4), 2014
PMID: 24141280
TestSTORM: Simulator for optimizing sample labeling and image acquisition in localization based super-resolution microscopy.
Sinkó J, Kákonyi R, Rees E, Metcalf D, Knight AE, Kaminski CF, Szabó G, Erdélyi M., Biomed Opt Express 5(3), 2014
PMID: 24688813
Membrane distribution of the glycine receptor α3 studied by optical super-resolution microscopy.
Notelaers K, Rocha S, Paesen R, Swinnen N, Vangindertael J, Meier JC, Rigo JM, Ameloot M, Hofkens J., Histochem Cell Biol 142(1), 2014
PMID: 24553792
Single-molecule super-resolution imaging by tryptophan-quenching-induced photoswitching of phalloidin-fluorophore conjugates.
Nanguneri S, Flottmann B, Herrmannsdörfer F, Thomas K, Heilemann M., Microsc Res Tech 77(7), 2014
PMID: 24595992
Collective fluorescence switching of counterion-assembled dyes in polymer nanoparticles.
Reisch A, Didier P, Richert L, Oncul S, Arntz Y, Mély Y, Klymchenko AS., Nat Commun 5(), 2014
PMID: 24909912
Accessing the third dimension in localization-based super-resolution microscopy.
Hajj B, El Beheiry M, Izeddin I, Darzacq X, Dahan M., Phys Chem Chem Phys 16(31), 2014
PMID: 24901106
Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging.
Geissbuehler S, Sharipov A, Godinat A, Bocchio NL, Sandoz PA, Huss A, Jensen NA, Jakobs S, Enderlein J, Gisou van der Goot F, Dubikovskaya EA, Lasser T, Leutenegger M., Nat Commun 5(), 2014
PMID: 25518894
Super-resolution imaging of EC coupling protein distribution in the heart.
Soeller C, Baddeley D., J Mol Cell Cardiol 58(), 2013
PMID: 23159441
Photoactivatable synthetic fluorophores.
Raymo FM., Phys Chem Chem Phys 15(36), 2013
PMID: 23780303
Test samples for optimizing STORM super-resolution microscopy.
Metcalf DJ, Edwards R, Kumarswami N, Knight AE., J Vis Exp (79), 2013
PMID: 24056752
Mechanism of intramolecular photostabilization in self-healing cyanine fluorophores.
van der Velde JH, Ploetz E, Hiermaier M, Oelerich J, de Vries JW, Roelfes G, Cordes T., Chemphyschem 14(18), 2013
PMID: 24302532
Synthesis and characterization of photoswitchable fluorescent SiO2 nanoparticles.
May F, Peter M, Hütten A, Prodi L, Mattay J., Chemistry 18(3), 2012
PMID: 22213584
Super-resolution fluorescence imaging of chromosomal DNA.
Zessin PJ, Finan K, Heilemann M., J Struct Biol 177(2), 2012
PMID: 22226957
Live-cell super-resolution imaging with synthetic fluorophores.
van de Linde S, Heilemann M, Sauer M., Annu Rev Phys Chem 63(), 2012
PMID: 22404589
Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting.
Veatch SL, Machta BB, Shelby SA, Chiang EN, Holowka DA, Baird BA., PLoS One 7(2), 2012
PMID: 22384026
Reversible fluorescence photoswitching in DNA.
Smith DA, Holliger P, Flors C., J Phys Chem B 116(34), 2012
PMID: 22861666
Single-molecule super-resolution imaging in bacteria.
Cattoni DI, Fiche JB, Nöllmann M., Curr Opin Microbiol 15(6), 2012
PMID: 23142583
Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging.
van de Linde S, Krstić I, Prisner T, Doose S, Heilemann M, Sauer M., Photochem Photobiol Sci 10(4), 2011
PMID: 21152594
Comparison between SOFI and STORM.
Geissbuehler S, Dellagiacoma C, Lasser T., Biomed Opt Express 2(3), 2011
PMID: 21412447
Improved temporal resolution and linked hidden Markov modeling for switchable single-molecule FRET.
Uphoff S, Gryte K, Evans G, Kapanidis AN., Chemphyschem 12(3), 2011
PMID: 21280168
Far-field nanoscopy with reversible chemical reactions.
Schwering M, Kiel A, Kurz A, Lymperopoulos K, Sprödefeld A, Krämer R, Herten DP., Angew Chem Int Ed Engl 50(13), 2011
PMID: 21404374
Measuring localization performance of super-resolution algorithms on very active samples.
Wolter S, Endesfelder U, van de Linde S, Heilemann M, Sauer M., Opt Express 19(8), 2011
PMID: 21503016
Kinetic studies on visible-light-switchable photochromic fluorophores based on diarylethenes.
Seefeldt B, Altenhöner K, Tosic O, Geisler T, Sauer M, Mattay J., Photochem Photobiol Sci 10(9), 2011
PMID: 21603721
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde S, Löschberger A, Klein T, Heidbreder M, Wolter S, Heilemann M, Sauer M., Nat Protoc 6(7), 2011
PMID: 21720313
Binding-activated localization microscopy of DNA structures.
Schoen I, Ries J, Klotzsch E, Ewers H, Vogel V., Nano Lett 11(9), 2011
PMID: 21838238
Optical imaging of nanoscale cellular structures.
Hedde PN, Nienhaus GU., Biophys Rev 2(4), 2010
PMID: 28510037


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 20176060
PubMed | Europe PMC

Suchen in

Google Scholar