Accurate determination of C-13 enrichments in nonprotonated carbon atoms of isotopically enriched amino acids by H-1 nuclear magnetic resonance

Wendisch VF, deGraaf AA, Sahm H (1997)
Analytical Biochemistry 245(2): 196-202.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
A method for the accurate determination of C-13 enrichments in nonprotonated carbon atoms of organic compounds that makes use of unresolved C-13 satellites of proton(s) bonded to the vicinal carbon atom was developed. Using glutamate as a model molecule, this H-1 nuclear magnetic resonance (NMR) inverse spin-echo difference spectroscopy method was calibrated for inversion efficiency and relaxation effects which were then shown to cause only a minor loss of the measured C-13 Satellite amplitude (2% for glutamate C-l and 7% for glutamate C-5). The determination of C-13 enrichments in nonprotonated glutamate carbon atoms by this method was shown to be more precise than C-13 MMR, As a first application, a [5-C-13]glucose labeling experiment with Corynebacterium glutamicum ASK1 was performed. The labeling patterns of glutamate and arginine extracted from cellular protein were determined using the newly developed method and standard H-1 NMR with and without broadband C-13 decoupling. Determination of the C-13 enrichment in C-5 of glutamate and arginine, respectively, by the two methods showed good agreement. From the deduced labeling pattern of a-oxoglutarate, an in vivo carbon flux distribution within the central metabolism of C. glutamicum ASK1 was calculated. Thus, the relative flux toward oxaloacetate via the tricarboxylic acid cycle enzyme malate dehydrogenase was determined as 45%, whereas that via anaplerotic C-3 carboxylation was determined as 55%. (C) 1997 Academic Press.
Erscheinungsjahr
Zeitschriftentitel
Analytical Biochemistry
Band
245
Ausgabe
2
Seite(n)
196-202
ISSN
PUB-ID

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Wendisch VF, deGraaf AA, Sahm H. Accurate determination of C-13 enrichments in nonprotonated carbon atoms of isotopically enriched amino acids by H-1 nuclear magnetic resonance. Analytical Biochemistry. 1997;245(2):196-202.
Wendisch, V. F., deGraaf, A. A., & Sahm, H. (1997). Accurate determination of C-13 enrichments in nonprotonated carbon atoms of isotopically enriched amino acids by H-1 nuclear magnetic resonance. Analytical Biochemistry, 245(2), 196-202.
Wendisch, V. F., deGraaf, A. A., and Sahm, H. (1997). Accurate determination of C-13 enrichments in nonprotonated carbon atoms of isotopically enriched amino acids by H-1 nuclear magnetic resonance. Analytical Biochemistry 245, 196-202.
Wendisch, V.F., deGraaf, A.A., & Sahm, H., 1997. Accurate determination of C-13 enrichments in nonprotonated carbon atoms of isotopically enriched amino acids by H-1 nuclear magnetic resonance. Analytical Biochemistry, 245(2), p 196-202.
V.F. Wendisch, A.A. deGraaf, and H. Sahm, “Accurate determination of C-13 enrichments in nonprotonated carbon atoms of isotopically enriched amino acids by H-1 nuclear magnetic resonance”, Analytical Biochemistry, vol. 245, 1997, pp. 196-202.
Wendisch, V.F., deGraaf, A.A., Sahm, H.: Accurate determination of C-13 enrichments in nonprotonated carbon atoms of isotopically enriched amino acids by H-1 nuclear magnetic resonance. Analytical Biochemistry. 245, 196-202 (1997).
Wendisch, Volker F., deGraaf, A. A., and Sahm, H. “Accurate determination of C-13 enrichments in nonprotonated carbon atoms of isotopically enriched amino acids by H-1 nuclear magnetic resonance”. Analytical Biochemistry 245.2 (1997): 196-202.

9 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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Impact of 'ome' analyses on inverse metabolic engineering.
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Disruption of the SHM2 gene, encoding one of two serine hydroxymethyltransferase isoenzymes, reduces the flux from glycine to serine in Ashbya gossypii.
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Strategies for metabolic flux analysis in plants using isotope labelling.
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