Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes

Sindelar G, Wendisch VF (2007)
Applied Microbiology and Biotechnology 76(3): 677-689.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
;
Abstract / Bemerkung
For the biotechnological production of L-lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement of carbon precursor supply and of nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH) regeneration, are known. To identify novel target genes to improve lysine production, the transcriptomes of the classically obtained lysine producing strain MH20-22B and several other C. glutamicum strains were compared. As lysine production by the classically obtained strain, which possesses feedback-resistant aspartokinase and is leucine auxotrophic, exceeds that of a genetically defined leucine auxotrophic wild-type derivative possessing feed-back-resistant aspartokinase, additional traits beneficial for lysine production are present. NCg10855, putatively encoding a methyltransferase, and theamt A-ocd-soxA operon, encoding an ammonium uptake system, a putative ornithine cyclodeaminase and an uncharacterized enzyme, were among the genes showing increased expression in the classically obtained strain irrespective of the presence of feedback-resistant aspartokinase. Lysine production could be improved by about 40% through overexpression of NCg10855 or the amtA-ocd-soxA operon. Thus, novel target genes for the improvement of lysine production could be identified in a discovery-driven approach based on global gene expression analysis.
Stichworte
nucleotide-sequence; glutamate production; biochemical-characterization; pyruvate-carboxylase; phosphoenolpyruvate carboxykinase; escherichia-coli; strain development; transcriptomics; DNA microarrays; corynebacterium glutamicum; l-lysine production; expression analysis; nitrogen regulation; genome sequence; central metabolism
Erscheinungsjahr
2007
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
76
Ausgabe
3
Seite(n)
677-689
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/1895273

Zitieren

Sindelar G, Wendisch VF. Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes. Applied Microbiology and Biotechnology. 2007;76(3):677-689.
Sindelar, G., & Wendisch, V. F. (2007). Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes. Applied Microbiology and Biotechnology, 76(3), 677-689. doi:10.1007/s00253-007-0916-x
Sindelar, G., and Wendisch, V. F. (2007). Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes. Applied Microbiology and Biotechnology 76, 677-689.
Sindelar, G., & Wendisch, V.F., 2007. Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes. Applied Microbiology and Biotechnology, 76(3), p 677-689.
G. Sindelar and V.F. Wendisch, “Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes”, Applied Microbiology and Biotechnology, vol. 76, 2007, pp. 677-689.
Sindelar, G., Wendisch, V.F.: Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes. Applied Microbiology and Biotechnology. 76, 677-689 (2007).
Sindelar, G., and Wendisch, Volker F. “Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes”. Applied Microbiology and Biotechnology 76.3 (2007): 677-689.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17364200
PubMed | Europe PMC

Suchen in

Google Scholar