Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis

Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF (2007)
Fems Microbiology Letters 273(1): 109-119.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Corynebacterium glutamicum grows aerobically on a variety of carbohydrates and organic acids as single or combined sources of carbon and energy. To characterize the citrate utilization in C. glutamicum on a genomewide scale, a comparative analysis was carried out by combining transcriptome and proteome analysis. In cells grown on citrate, transcriptome analysis revealed highest expression changes for two different citrate-uptake systems encoded by citM and tctCBA, whereas genes encoding uptake systems for the glucose- (ptsG), sucrose- (ptsS) and fructose- (ptsF) specific PTS components and permeases for gluconate (gntP) and glutamate (gluC) displayed decreased mRNA levels in citrate-grown cells. This pattern was also observed when cells grown in Luria-Bertani (LB) medium plus citrate were compared with cells grown in LB medium, indicating some kind of catabolite repression. Genes encoding enzymes of the tricarboxylic acid cycle (aconitase, succinyl-CoA synthetase, succinate dehydrogenase and fumarase), malic enzyme, PEP carboxykinase, gluconeogenic glyceraldehyde-3-phosphate dehydrogenase and ATP synthase displayed increased expression in cells grown on citrate. Accordingly, proteome analysis revealed elevated protein levels of these enzymes and showed a good correlation with the mRNA levels. In conclusion, this study revealed the citrate stimulon in C. glutamicum and the regulated central metabolic genes when grown on citrate.
Stichworte
gene; regulator; growth; expression; corynebacterium glutamicum; citrate; utilization; acetate metabolism; transcriptomics; proteomics; citric acid cycle; biochemical-characterization; glyoxylate bypass; carbon-sources; amino-acids; identification
Erscheinungsjahr
2007
Zeitschriftentitel
Fems Microbiology Letters
Band
273
Ausgabe
1
Seite(n)
109-119
ISSN
0378-1097
eISSN
1574-6968
Page URI
https://pub.uni-bielefeld.de/record/1895218

Zitieren

Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF. Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters. 2007;273(1):109-119.
Polen, T., Schluesener, D., Poetsch, A., Bott, M., & Wendisch, V. F. (2007). Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters, 273(1), 109-119. doi:10.1111/j.1574-6968.2007.00793.x
Polen, T., Schluesener, D., Poetsch, A., Bott, M., and Wendisch, V. F. (2007). Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters 273, 109-119.
Polen, T., et al., 2007. Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters, 273(1), p 109-119.
T. Polen, et al., “Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis”, Fems Microbiology Letters, vol. 273, 2007, pp. 109-119.
Polen, T., Schluesener, D., Poetsch, A., Bott, M., Wendisch, V.F.: Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters. 273, 109-119 (2007).
Polen, T., Schluesener, D., Poetsch, A., Bott, M., and Wendisch, Volker F. “Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis”. Fems Microbiology Letters 273.1 (2007): 109-119.

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