Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis
Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF (2007)
Fems Microbiology Letters 273(1): 109-119.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Polen, T.;
Schluesener, D.;
Poetsch, A.;
Bott, M.;
Wendisch, Volker F.UniBi ![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Einrichtung
Abstract / Bemerkung
Corynebacterium glutamicum grows aerobically on a variety of carbohydrates and organic acids as single or combined sources of carbon and energy. To characterize the citrate utilization in C. glutamicum on a genomewide scale, a comparative analysis was carried out by combining transcriptome and proteome analysis. In cells grown on citrate, transcriptome analysis revealed highest expression changes for two different citrate-uptake systems encoded by citM and tctCBA, whereas genes encoding uptake systems for the glucose- (ptsG), sucrose- (ptsS) and fructose- (ptsF) specific PTS components and permeases for gluconate (gntP) and glutamate (gluC) displayed decreased mRNA levels in citrate-grown cells. This pattern was also observed when cells grown in Luria-Bertani (LB) medium plus citrate were compared with cells grown in LB medium, indicating some kind of catabolite repression. Genes encoding enzymes of the tricarboxylic acid cycle (aconitase, succinyl-CoA synthetase, succinate dehydrogenase and fumarase), malic enzyme, PEP carboxykinase, gluconeogenic glyceraldehyde-3-phosphate dehydrogenase and ATP synthase displayed increased expression in cells grown on citrate. Accordingly, proteome analysis revealed elevated protein levels of these enzymes and showed a good correlation with the mRNA levels. In conclusion, this study revealed the citrate stimulon in C. glutamicum and the regulated central metabolic genes when grown on citrate.
Stichworte
gene;
regulator;
growth;
expression;
corynebacterium glutamicum;
citrate;
utilization;
acetate metabolism;
transcriptomics;
proteomics;
citric acid cycle;
biochemical-characterization;
glyoxylate bypass;
carbon-sources;
amino-acids;
identification
Erscheinungsjahr
2007
Zeitschriftentitel
Fems Microbiology Letters
Band
273
Ausgabe
1
Seite(n)
109-119
ISSN
0378-1097
eISSN
1574-6968
Page URI
https://pub.uni-bielefeld.de/record/1895218
Zitieren
Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF. Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters. 2007;273(1):109-119.
Polen, T., Schluesener, D., Poetsch, A., Bott, M., & Wendisch, V. F. (2007). Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters, 273(1), 109-119. https://doi.org/10.1111/j.1574-6968.2007.00793.x
Polen, T., Schluesener, D., Poetsch, A., Bott, M., and Wendisch, Volker F. 2007. “Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis”. Fems Microbiology Letters 273 (1): 109-119.
Polen, T., Schluesener, D., Poetsch, A., Bott, M., and Wendisch, V. F. (2007). Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters 273, 109-119.
Polen, T., et al., 2007. Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters, 273(1), p 109-119.
T. Polen, et al., “Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis”, Fems Microbiology Letters, vol. 273, 2007, pp. 109-119.
Polen, T., Schluesener, D., Poetsch, A., Bott, M., Wendisch, V.F.: Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. Fems Microbiology Letters. 273, 109-119 (2007).
Polen, T., Schluesener, D., Poetsch, A., Bott, M., and Wendisch, Volker F. “Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis”. Fems Microbiology Letters 273.1 (2007): 109-119.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
38 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Efficient Production of the Dicarboxylic Acid Glutarate by Corynebacterium glutamicum via a Novel Synthetic Pathway.
Pérez-García F, Jorge JMP, Dreyszas A, Risse JM, Wendisch VF., Front Microbiol 9(), 2018
PMID: 30425699
Pérez-García F, Jorge JMP, Dreyszas A, Risse JM, Wendisch VF., Front Microbiol 9(), 2018
PMID: 30425699
Improved fermentative production of gamma-aminobutyric acid via the putrescine route: Systems metabolic engineering for production from glucose, amino sugars, and xylose.
Jorge JM, Nguyen AQ, Pérez-García F, Kind S, Wendisch VF., Biotechnol Bioeng 114(4), 2017
PMID: 27800627
Jorge JM, Nguyen AQ, Pérez-García F, Kind S, Wendisch VF., Biotechnol Bioeng 114(4), 2017
PMID: 27800627
Identification of the cAMP phosphodiesterase CpdA as novel key player in cAMP-dependent regulation in Corynebacterium glutamicum.
Schulte J, Baumgart M, Bott M., Mol Microbiol 103(3), 2017
PMID: 27862445
Schulte J, Baumgart M, Bott M., Mol Microbiol 103(3), 2017
PMID: 27862445
Overexpression of the primary sigma factor gene sigA improved carotenoid production by Corynebacterium glutamicum: Application to production of β-carotene and the non-native linear C50 carotenoid bisanhydrobacterioruberin.
Taniguchi H, Henke NA, Heider SAE, Wendisch VF., Metab Eng Commun 4(), 2017
PMID: 29142827
Taniguchi H, Henke NA, Heider SAE, Wendisch VF., Metab Eng Commun 4(), 2017
PMID: 29142827
A new metabolic route for the fermentative production of 5-aminovalerate from glucose and alternative carbon sources.
Jorge JMP, Pérez-García F, Wendisch VF., Bioresour Technol 245(pt b), 2017
PMID: 28522202
Jorge JMP, Pérez-García F, Wendisch VF., Bioresour Technol 245(pt b), 2017
PMID: 28522202
Regulons of global transcription factors in Corynebacterium glutamicum.
Toyoda K, Inui M., Appl Microbiol Biotechnol 100(1), 2016
PMID: 26496920
Toyoda K, Inui M., Appl Microbiol Biotechnol 100(1), 2016
PMID: 26496920
A new metabolic route for the production of gamma-aminobutyric acid by Corynebacterium glutamicum from glucose.
Jorge JM, Leggewie C, Wendisch VF., Amino Acids 48(11), 2016
PMID: 27289384
Jorge JM, Leggewie C, Wendisch VF., Amino Acids 48(11), 2016
PMID: 27289384
The small 6C RNA of Corynebacterium glutamicum is involved in the SOS response.
Pahlke J, Dostálová H, Holátko J, Degner U, Bott M, Pátek M, Polen T., RNA Biol 13(9), 2016
PMID: 27362471
Pahlke J, Dostálová H, Holátko J, Degner U, Bott M, Pátek M, Polen T., RNA Biol 13(9), 2016
PMID: 27362471
Transcription of Sialic Acid Catabolism Genes in Corynebacterium glutamicum Is Subject to Catabolite Repression and Control by the Transcriptional Repressor NanR.
Uhde A, Brühl N, Goldbeck O, Matano C, Gurow O, Rückert C, Marin K, Wendisch VF, Krämer R, Seibold GM., J Bacteriol 198(16), 2016
PMID: 27274030
Uhde A, Brühl N, Goldbeck O, Matano C, Gurow O, Rückert C, Marin K, Wendisch VF, Krämer R, Seibold GM., J Bacteriol 198(16), 2016
PMID: 27274030
Ciprofloxacin triggered glutamate production by Corynebacterium glutamicum.
Lubitz D, Wendisch VF., BMC Microbiol 16(1), 2016
PMID: 27717325
Lubitz D, Wendisch VF., BMC Microbiol 16(1), 2016
PMID: 27717325
Advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products.
Becker J, Wittmann C., Angew Chem Int Ed Engl 54(11), 2015
PMID: 25684732
Becker J, Wittmann C., Angew Chem Int Ed Engl 54(11), 2015
PMID: 25684732
Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB.
Sorger-Herrmann U, Taniguchi H, Wendisch VF., BMC Microbiol 15(), 2015
PMID: 26021728
Sorger-Herrmann U, Taniguchi H, Wendisch VF., BMC Microbiol 15(), 2015
PMID: 26021728
Exploring the role of sigma factor gene expression on production by Corynebacterium glutamicum: sigma factor H and FMN as example.
Taniguchi H, Wendisch VF., Front Microbiol 6(), 2015
PMID: 26257719
Taniguchi H, Wendisch VF., Front Microbiol 6(), 2015
PMID: 26257719
Single-Domain Peptidyl-Prolyl cis/trans Isomerase FkpA from Corynebacterium glutamicum Improves the Biomass Yield at Increased Growth Temperatures.
Kallscheuer N, Bott M, van Ooyen J, Polen T., Appl Environ Microbiol 81(22), 2015
PMID: 26341203
Kallscheuer N, Bott M, van Ooyen J, Polen T., Appl Environ Microbiol 81(22), 2015
PMID: 26341203
Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum.
Leßmeier L, Wendisch VF., BMC Microbiol 15(), 2015
PMID: 26474849
Leßmeier L, Wendisch VF., BMC Microbiol 15(), 2015
PMID: 26474849
Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction.
Vogt M, Haas S, Klaffl S, Polen T, Eggeling L, van Ooyen J, Bott M., Metab Eng 22(), 2014
PMID: 24333966
Vogt M, Haas S, Klaffl S, Polen T, Eggeling L, van Ooyen J, Bott M., Metab Eng 22(), 2014
PMID: 24333966
Subtilase SprP exerts pleiotropic effects in Pseudomonas aeruginosa.
Pelzer A, Polen T, Funken H, Rosenau F, Wilhelm S, Bott M, Jaeger KE., Microbiologyopen 3(1), 2014
PMID: 24376018
Pelzer A, Polen T, Funken H, Rosenau F, Wilhelm S, Bott M, Jaeger KE., Microbiologyopen 3(1), 2014
PMID: 24376018
Metabolic engineering of Corynebacterium glutamicum for glycolate production.
Zahoor A, Otten A, Wendisch VF., J Biotechnol 192 Pt B(), 2014
PMID: 24486442
Zahoor A, Otten A, Wendisch VF., J Biotechnol 192 Pt B(), 2014
PMID: 24486442
A novel TctA citrate transporter from an activated sludge metagenome: structural and mechanistic predictions for the TTT family.
Batista-García RA, Sánchez-Reyes A, Millán-Pacheco C, González-Zuñiga VM, Juárez S, Folch-Mallol JL, Pastor N., Proteins 82(9), 2014
PMID: 24493659
Batista-García RA, Sánchez-Reyes A, Millán-Pacheco C, González-Zuñiga VM, Juárez S, Folch-Mallol JL, Pastor N., Proteins 82(9), 2014
PMID: 24493659
Glucosamine as carbon source for amino acid-producing Corynebacterium glutamicum.
Uhde A, Youn JW, Maeda T, Clermont L, Matano C, Krämer R, Wendisch VF, Seibold GM, Marin K., Appl Microbiol Biotechnol 97(4), 2013
PMID: 22854894
Uhde A, Youn JW, Maeda T, Clermont L, Matano C, Krämer R, Wendisch VF, Seibold GM, Marin K., Appl Microbiol Biotechnol 97(4), 2013
PMID: 22854894
Crystal and solution studies reveal that the transcriptional regulator AcnR of Corynebacterium glutamicum is regulated by citrate-Mg2+ binding to a non-canonical pocket.
García-Nafría J, Baumgart M, Turkenburg JP, Wilkinson AJ, Bott M, Wilson KS., J Biol Chem 288(22), 2013
PMID: 23589369
García-Nafría J, Baumgart M, Turkenburg JP, Wilkinson AJ, Bott M, Wilson KS., J Biol Chem 288(22), 2013
PMID: 23589369
Proteome response of Corynebacterium glutamicum to high concentration of industrially relevant C₄ and C₅ dicarboxylic acids.
Vasco-Cárdenas MF, Baños S, Ramos A, Martín JF, Barreiro C., J Proteomics 85(), 2013
PMID: 23624027
Vasco-Cárdenas MF, Baños S, Ramos A, Martín JF, Barreiro C., J Proteomics 85(), 2013
PMID: 23624027
Complex regulation of the phosphoenolpyruvate carboxykinase gene pck and characterization of its GntR-type regulator IolR as a repressor of myo-inositol utilization genes in Corynebacterium glutamicum.
Klaffl S, Brocker M, Kalinowski J, Eikmanns BJ, Bott M., J Bacteriol 195(18), 2013
PMID: 23873914
Klaffl S, Brocker M, Kalinowski J, Eikmanns BJ, Bott M., J Bacteriol 195(18), 2013
PMID: 23873914
Two-component signal transduction in Corynebacterium glutamicum and other corynebacteria: on the way towards stimuli and targets.
Bott M, Brocker M., Appl Microbiol Biotechnol 94(5), 2012
PMID: 22539022
Bott M, Brocker M., Appl Microbiol Biotechnol 94(5), 2012
PMID: 22539022
The yctCBA operon of Yersinia ruckeri, involved in in vivo citrate uptake, is not required for virulence.
Navais R, Méndez J, Reimundo P, Pérez-Pascual D, Gómez E, Guijarro JA., Appl Environ Microbiol 77(3), 2011
PMID: 21131526
Navais R, Méndez J, Reimundo P, Pérez-Pascual D, Gómez E, Guijarro JA., Appl Environ Microbiol 77(3), 2011
PMID: 21131526
Phosphotransferase system-independent glucose utilization in corynebacterium glutamicum by inositol permeases and glucokinases.
Lindner SN, Seibold GM, Henrich A, Krämer R, Wendisch VF., Appl Environ Microbiol 77(11), 2011
PMID: 21478323
Lindner SN, Seibold GM, Henrich A, Krämer R, Wendisch VF., Appl Environ Microbiol 77(11), 2011
PMID: 21478323
Genome-wide identification of in vivo binding sites of GlxR, a cyclic AMP receptor protein-type regulator in Corynebacterium glutamicum.
Toyoda K, Teramoto H, Inui M, Yukawa H., J Bacteriol 193(16), 2011
PMID: 21665967
Toyoda K, Teramoto H, Inui M, Yukawa H., J Bacteriol 193(16), 2011
PMID: 21665967
Proteomics of corynebacteria: From biotechnology workhorses to pathogens.
Poetsch A, Haussmann U, Burkovski A., Proteomics 11(15), 2011
PMID: 21674800
Poetsch A, Haussmann U, Burkovski A., Proteomics 11(15), 2011
PMID: 21674800
Deletion of the aconitase gene in Corynebacterium glutamicum causes strong selection pressure for secondary mutations inactivating citrate synthase.
Baumgart M, Mustafi N, Krug A, Bott M., J Bacteriol 193(24), 2011
PMID: 21984793
Baumgart M, Mustafi N, Krug A, Bott M., J Bacteriol 193(24), 2011
PMID: 21984793
Evolution: like any other science it is predictable.
Morris SC., Philos Trans R Soc Lond B Biol Sci 365(1537), 2010
PMID: 20008391
Morris SC., Philos Trans R Soc Lond B Biol Sci 365(1537), 2010
PMID: 20008391
Transcriptional regulation of gene expression in Corynebacterium glutamicum: the role of global, master and local regulators in the modular and hierarchical gene regulatory network.
Schröder J, Tauch A., FEMS Microbiol Rev 34(5), 2010
PMID: 20491930
Schröder J, Tauch A., FEMS Microbiol Rev 34(5), 2010
PMID: 20491930
Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate.
Kato O, Youn JW, Stansen KC, Matsui D, Oikawa T, Wendisch VF., BMC Microbiol 10(), 2010
PMID: 21159175
Kato O, Youn JW, Stansen KC, Matsui D, Oikawa T, Wendisch VF., BMC Microbiol 10(), 2010
PMID: 21159175
Citrate utilization by Corynebacterium glutamicum is controlled by the CitAB two-component system through positive regulation of the citrate transport genes citH and tctCBA.
Brocker M, Schaffer S, Mack C, Bott M., J Bacteriol 191(12), 2009
PMID: 19376865
Brocker M, Schaffer S, Mack C, Bott M., J Bacteriol 191(12), 2009
PMID: 19376865
Physiological adaptation of Corynebacterium glutamicum to benzoate as alternative carbon source - a membrane proteome-centric view.
Haussmann U, Qi SW, Wolters D, Rögner M, Liu SJ, Poetsch A., Proteomics 9(14), 2009
PMID: 19639586
Haussmann U, Qi SW, Wolters D, Rögner M, Liu SJ, Poetsch A., Proteomics 9(14), 2009
PMID: 19639586
Characterization of the dicarboxylate transporter DctA in Corynebacterium glutamicum.
Youn JW, Jolkver E, Krämer R, Marin K, Wendisch VF., J Bacteriol 191(17), 2009
PMID: 19581365
Youn JW, Jolkver E, Krämer R, Marin K, Wendisch VF., J Bacteriol 191(17), 2009
PMID: 19581365
The GlxR regulon of the amino acid producer Corynebacterium glutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model.
Kohl TA, Tauch A., J Biotechnol 143(4), 2009
PMID: 19665500
Kohl TA, Tauch A., J Biotechnol 143(4), 2009
PMID: 19665500
Identification and characterization of the dicarboxylate uptake system DccT in Corynebacterium glutamicum.
Youn JW, Jolkver E, Krämer R, Marin K, Wendisch VF., J Bacteriol 190(19), 2008
PMID: 18658264
Youn JW, Jolkver E, Krämer R, Marin K, Wendisch VF., J Bacteriol 190(19), 2008
PMID: 18658264
Response of the cytoplasmic and membrane proteome of Corynebacterium glutamicum ATCC 13032 to pH changes.
Barriuso-Iglesias M, Schluesener D, Barreiro C, Poetsch A, Martín JF., BMC Microbiol 8(), 2008
PMID: 19091079
Barriuso-Iglesias M, Schluesener D, Barreiro C, Poetsch A, Martín JF., BMC Microbiol 8(), 2008
PMID: 19091079
39 References
Daten bereitgestellt von Europe PubMed Central.
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
Eikmanns, 2005
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
Sambrook, 1989
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 17559405
PubMed | Europe PMC
Suchen in